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Citations for New England Biolabs :
601 - 650 of 3976 citations for Red colored Polystyrene Beads 1 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 20 μg of recombinant PbChiB2 and PbChiB4 resuspended in 20 mM Tris (pH 8.0) were independently mixed with 2 mg of chitin magnetic beads (NEB, CAD), chitin from shrimp shell (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Enrichment of mRNA from eukaryotic organisms was performed using oligo(dT) beads from NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat.No. E7490L, NEB, USA). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... using 10 μl of the bead suspension in a 50 μl reaction with NEBNext Ultra II Q5 2x master mix (NEB) and 0.5 μM each Solexa 1GA/1GB primers (Solexa 1GA ...
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bioRxiv - Microbiology 2020Quote: ... A-tails were added to the 3′ ends of the DNA by incubating the beads with Klenow fragment (exo-) (NEB) in a 100 μL reaction ...
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bioRxiv - Immunology 2021Quote: ... a flow-focusing device was used to encapsulate individual T cells into emulsification droplets containing lysis buffer and oligo(dT) magnetic beads (New England Biolabs)30 ...
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bioRxiv - Microbiology 2022Quote: ... Short read libraries were prepared based on the NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads (NEB) and sequenced using Illumina Miseq v3 (150 cycles) ...
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bioRxiv - Microbiology 2020Quote: ... Circularised product is subjected to two rounds of rRNA depletion using biotinylated oligos [17] and streptavidin-coated magnetic beads (NEB) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was submitted to mRNA Isolation and RNA fragmentation with NEBNext Oligo d(T)25 beads (NEW ENGLAND BioLabs) and cDNA synthesis ...
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bioRxiv - Cancer Biology 2020Quote: ... eluted in 8 μl and in vitro transcribed (with the beads) at 37 °C overnight for linear amplification using the T7 High Yield RNA polymerase IVT kit (NEB). Following IVT ...
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bioRxiv - Genomics 2020Quote: ... Double-stranded cDNAs were washed with XP Beads then were handled with NEBNext Ultra II End-prep enzyme mix (New England Biolabs). After an XP Bead-washing ...
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bioRxiv - Plant Biology 2021Quote: ... The solution was split into two halves and mRNAs were isolated from each using 150 μL magnetic Oligo(dT)25 beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sheared fragments were evenly distributed between 100 bp and 1500 bp and fragments of ~600 bp were selected using Agencourt AMPure XP Beads (New England Biolabs) following a protocol of double-size selection ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB, E7765S). Sequencing was carried out at the Max Planck Genome Centre (Cologne ...
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bioRxiv - Microbiology 2020Quote: ... before resuspending the beads in 25 μl RNA ligation mix (9 μl H2O, 3 μl 10x T4 RNA ligase buffer (NEB), 0.3 μl 0.1M ATP ...
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bioRxiv - Immunology 2021Quote: For RNA-seq library preparation 2ug of total RNA was used to isolate mRNA using magnetic beads with mRNA isolation kit (PolyA mRNA isolation module, NEB). Later mRNA library preparation kit ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RAD fragments were enriched with a streptavidin bead pull-down and prepared as a sequencing library using a NEBNext Ultra DNA Library Prep Kit (New England Biolabs). Final libraries were sequenced using paired-end 150 bp sequencing on an Illumina HiSeq 4000 at Duke University's Center for Genomic sand Computational Biology sequencing facility.
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bioRxiv - Microbiology 2023Quote: ... 200 μl reactions were incubated with 100 μl Ni-NTA beads equilibrated in 800 μl Ni-NTA buffer A with 10 mM imidazole and 40 U murine RNase inhibitor (NEB) at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... and library prep performed on the beads using the NEBNext Ultra II DNA library prep kit for Illumina (NEB, E7645L), according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... On-bead RNase H treatment control was performed for each reaction: washed beads were incubated with 300 µL 1X RNase H Buffer (NEB) plus 30 units of RNase H (NEB ...
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bioRxiv - Microbiology 2023Quote: ... and eukaryotic mRNA was removed by binding and discarding the eukaryotic mRNA polyA region to oligo d(T)25 magnetic beads (England Biolabs). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... and prey (60 pmol for Retriever or 500 pmol for DENND10) were mixed with 20 µL of Amylose beads (New England Biolabs) in 1 mL of binding buffer [10 mM HEPES pH 7 ...
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bioRxiv - Immunology 2023Quote: ... mRNAs were enriched by incubation with Oligo d(T) Magnetic Beads (New England Biolabs, Cat. No. S1419S, Ipswich, MA, USA) and then fragmented/eluted by incubation at 94°C for 9 min ...
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bioRxiv - Molecular Biology 2023Quote: Equilibration buffer (GF150 buffer supplemented with 0.1 mM Mg-ATP and 10% glycerol) was used to equilibrate 20 μL of SNAP-Capture Magnetic Beads (New England Biolabs) slurry in 1.5 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was enriched from crude cell lysate in two rounds using biotinylated oligo-dT (IDT Europe) and streptavidin beads (New England Biolabs). RNA isolations were performed in batches in a randomized order ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 μg of total RNAs from 12-day-old Arabidopsis leaves were extracted using the Trizol method and incubated with 30 μl of pre-equilibrated oligo d(T)25 magnetic beads (NEB) for 15 minutes at room temperature with continuous rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... and mRNA fragments with a poly(A) tail were isolated by Oligo d(T)25 Magnetic Beads (NEB Biolab, S1419S). Illumina sequence adaptors were added during reverse transcription with SMRT methods (Wang et al. ...
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bioRxiv - Biochemistry 2024Quote: ... 4SU-labeled RNA (100 µg) was biotinylated with HPDP-biotin (cat. 21341, ThermoScientific) and captured on 150 µL of streptavidin magnetic beads (NEB) per RNA sample ...
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bioRxiv - Genomics 2024Quote: ... All subsequent steps were performed on that bead-bound DNA fraction including DNA ends repair with repair mix (10Х ligase NEB buffer ...
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bioRxiv - Microbiology 2024Quote: ... Polyadenylated RNA was then isolated from the whole-cell RNA using NEB Oligo d(T)25 Magnetic beads (NEB, S1419S) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2023Quote: RNA was released from the beads through protein K lysis (Monarch® Total RNA Miniprep Kit; New England Biolabs, #T2010S). After lysis ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB E7765S). RNA-seq libraries for each sample type were prepared ...
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bioRxiv - Microbiology 2023Quote: ... Double stranded on-bead DNA was digested with a mix of 3 blunt cutting enzymes (SspI-HF, StuI, and HincII) (NEB) to generate blunt-end DNA ...
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bioRxiv - Biochemistry 2023Quote: ... The duplex hRNase 4 cleavage products (5′ fragments of the RNA) were enriched using Hydrophilic Streptavidin Magnetic Beads (New England Biolabs). The beads were washed twice by a high salt buffer (5 mM Tris HCl ...
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bioRxiv - Biophysics 2023Quote: Magnetic tweezers force calibration was performed as described previously70 using transverse fluctuations of a magnetic bead tethered to a glass coverslip via lambda phage DNA (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... myc-tagged AP3B1 immunoprecipitated and immobilised on Protein G-sepharose beads was incubated with CK2 (250 units; New England Biolabs) for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... To enrich for fully assembled TC-TL complexes the expressome was purified via immobilizing the 50S subunit (ZS22) onto streptavidin magnetic beads (NEB). For this ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified DNA was then size enriched using 0.9X volume SPRI beads and subject to another round of PCR using 10x cycles with NEBNext Ultra II (NEB M0544S) using primers pairs cTF383+cTF399 for the promoter and oSA021+oSA023 for the gene body to add Illumina Read1 and Read2 overhangs ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then transferred to a fresh low-bind tube containing pre-washed 5 µl amylose magnetic beads (NEB) and rotated for 45 min at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction was isolated from 4.5 μg of DNAse-treated total RNA using NEBNext Oligo d(T)25 Magnetic beads kit (NEB, USA), according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... on-bead decapping and phosphorylation were performed in a 30 μl reaction with 5 units T4 PNK 3’ phosphatase minus (NEB), 2.25 μg GST-Dcp1-Edc1-Dcp2 ...
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bioRxiv - Cell Biology 2022Quote: ... the beads were resuspended in TEV buffer (final volume 5 mL) with 100 μL TEV protease (New England BioLabs, #P8112S) and incubated at 4°C on a roller overnight ...
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bioRxiv - Genomics 2022Quote: ... The DNA was then purified with AMPure XP beads (Beckmann Coulter) and ligated with sequencing adapters (ONT) using NEBNext Quick T4 DNA Ligase (NEB). After purification with AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins from the supernatant at 2 mg/mL were used for the Co-IP assay using Protein G magnetic beads (New England Biolabs) and a mouse monoclonal anti-HA antibody (Sigma) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Beads were then dried before adding 50 µL DNaseI mix (3 U of DNaseI in 1X DNAse buffer (NEB, #M0303L) and incubated at 37 °C for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequent steps of RNA-seq were outsourced to Azenta who generated sequencing libraries from polyA-enriched RNA (captured with oligo-dT beads) using the NEBNext Ultra II RNA library prep kit for Illumina (NEB), multiplexed them ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... with prior enrichment of mRNA by using Oligo dT beads from the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). After library preparation ...
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bioRxiv - Molecular Biology 2022Quote: Polyadenylated RNA was purified from the DNase-treated total RNA using the oligo d(T)25 magnetic beads (NEB, S1419) and used for the library preparation ...
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bioRxiv - Biochemistry 2023Quote: ... Peptide bound beads were washed with 100 µL 1x lambda phosphatase buffer prior to incubating with lambda protein phosphatase (P0753; NEB).
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bioRxiv - Immunology 2023Quote: ... was biotinylated with sulfosuccinimidyl-6-[biotinamido]-6-hexanamido hexanoate (sulfo-NHS-LC-LC biotin; ThermoScientific) and coupled to streptavidin beads (New England Biolabs). Patient samples were incubated with RBD-coupled beads and excess sera washed off with PBS (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... A-tailing and Illumina adaptors ligation were performed on the beads using NEBNext library preparation end-repair and A-tailing modules (NEB). Libraries were amplified and sequenced in biological replicates ...