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Citations for New England Biolabs :
701 - 750 of 2049 citations for 6 Benzyloxy 1H indole 2 boronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we performed 2 reactions per sample (20 µl 5× NEB Q5 buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 U of DNase I-XT (New England Biolabs, USA) was added into 10 μL of reaction mixture and incubation was extended for 30 min at 37°C to remove the DNA templates ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... and samples were then individually diluted in NEBuffer 2 (NEB) and fragmented by incubating at 92 °C for 1.5 min ...
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bioRxiv - Microbiology 2024Quote: ... lysates were treated with 2 units of Proteinase K (NEB) for 30min ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µl T7 RNA Polymerase (New England BioLabs, Ipswich, MA), 5 µl ATP (10 μmol) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 µl of RNase H Buffer (New England Biolabs) was added and incubated at 45°C for 30 min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cas12a RNP: (13 µl water; 2 µl r2.1 buffer [NEB, Cat ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 2 units of Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer ...
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bioRxiv - Systems Biology 2024Quote: ... 2 μL of T4 DNA Polymerase (3 U/μL, NEB) 1 μL of Klenow DNA Polymerase (5 U/μL ...
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bioRxiv - Systems Biology 2024Quote: ... and 1 μL of Phusion Polymerase (2 U/μL, NEB) were added and mixed by pipette ...
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bioRxiv - Systems Biology 2024Quote: ... and 2 μL of DNA Polymerase I (10U/μL, NEB) were added and mixed by pipetting ...
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bioRxiv - Systems Biology 2024Quote: ... and 2 μL of T4 PNK (10 U/μL, NEB) were added ...
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High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... treated with 2 µL DNase I (New England Biolabs, M0303), and incubated at 37°C for 30 min to degrade the IVT PCR template DNA ...
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bioRxiv - Microbiology 2024Quote: ... burgdorferi cells using QuickLoad 2× Taq Master Mix (NEB, M0271). Positive clones were flash frozen on dry ice in BSK-II containing 10% DMSO (Sigma ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2 µL of 20 µM SpCas9 (New England Biolabs M0646T) were gently mixed with 2 µL of 100 µM gRNA ...
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bioRxiv - Genomics 2024Quote: ... was added to the gDNA in 1x NEBuffer 2 (NEB) in a final volume of 25 µL and incubated for 30-min at 37°C ...
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bioRxiv - Genetics 2024Quote: ... 2 μL of T4 Ligase buffer (New England Biolabs, USA) and 1 μL of T4 Polynucleotide Kinase (10 U/μL ...
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bioRxiv - Biophysics 2024Quote: ... before annealing and ligated using T4 RNA ligase 2 (NEB). Constructs containing 30 nt gaps ...
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bioRxiv - Genomics 2024Quote: ... and 50 μL of NEBNext 2× Master Mix (NEB M0541S). oGH840.1-6 are six oligonucleotides with variable lengths to stagger and phase the sequencing of a common sequence in the target site amplicon ...
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bioRxiv - Genetics 2024Quote: ... and digested with 2 μl of β-agarase I (NEB) at 42 °C for 90 min ...
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bioRxiv - Biochemistry 2024Quote: ... 2 units of either DNase I or T7 Exonuclease (NEB) were then added ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μL of T4 DNA ligase (NEB, 400000 U/mL), and 4 μL of 10X T4 DNA ligase buffer (NEB) ...
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bioRxiv - Genetics 2024Quote: ... using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). We inserted these three fragments into the pLS-SceI plasmid (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... together with 2 µM of Cas9 protein (NEB, Cat# M0646T). Tol2-plasmids were diluted in 0.05% Phenol red to a final concentration of 100 ng/µL ...
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bioRxiv - Evolutionary Biology 2021Quote: 5’ phosphorylation was performed by mixing 6 uL of rRNA depleted RNA with 1 uL of 10X PNK buffer (NEB B0201S), 1 uL of PNK enzyme (NEB M0236S) ...
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bioRxiv - Systems Biology 2021Quote: ... PE2 indexing barcodes were then added by amplifying 2 microliters of the previous reaction with forward primer P1_outer and reverse primers PE2_outer_SIC69 and PE2_outer_SIC70 (Supplementary file 6) for 5 cycles at an annealing temperature of 66C followed by 5 cycles with no annealing step (NEB Q5) and then purified with the Monarch PCR kit.
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bioRxiv - Systems Biology 2021Quote: ... P1 indexing barcodes were added using forward primers P1_inner_A through P1_inner_D and reverse primer P1_inner_nested_rev (Supplementary file 6) for 5 cycles at an annealing temperature of 55C followed by 5 cycles with no annealing step (NEB Q5). PE2 indexing barcodes were then added by amplifying 2 microliters of the previous reaction with forward primer P1_outer and reverse primers PE2_outer_SIC69 and PE2_outer_SIC70 (Supplementary file 6 ...
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bioRxiv - Systems Biology 2021Quote: ... The final enrichment PCR used primers MO588 and MO589 (Supplementary file 6) for 20 cycles at an annealing temperature of 66C (NEB Phusion), followed by purification with the Monarch PCR kit ...
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bioRxiv - Cancer Biology 2020Quote: ... and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences) using the Luna Universal qPCR kit (New England Biolabs, M3003). Relative expression was normalized to Actb and Gapdh housekeeping genes and was determined using the ΔΔCt method ...
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bioRxiv - Genomics 2020Quote: ... and then used as the template for an additional 6 cycles of PCR amplification with NEBNext i5 and i7 primers (NEB #E7600S).
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bioRxiv - Genomics 2021Quote: NGS libraries were generated by amplifying the CUT&Tag DNA fragments with i5 and i7 barcoded HPLC-grade primers (Buenrostro et al., 2015) (Supplementary Table 6) with NEBNext® HiFi 2x PCR Master Mix (New England BioLabs) on a thermocycler with the following program ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription reactions were performed with 6 μl of purified RNA and random primers using the NEB ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 510 ng (6 µl at 85 ng/µl) of DNA was digested using endonucleases EcoRI and MseI (New England BioLabs, Inc.) after which barcoded (EcoRI cut site ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously (33) ...
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bioRxiv - Cell Biology 2023Quote: ... For second strand cDNA synthesis 6 μL of second strand reaction mix was added [1× NEBNext Second Strand Synthesis buffer (NEB #E6111S), NEBNext Second Strand Synthesis Enzyme Mix ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNA barcodes were amplified by PCR using the gDNA from at least 6 mio cells as template and Phusion (NEB M0530) as polymerase ...
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bioRxiv - Genomics 2022Quote: ... a donor template with the EGFP-stop-codon cassette including a 6 times glycine-alanine spacer sequence was cloned in between the two homology arms by using MluI (NEB: R0198S) and BglII (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously 45.
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bioRxiv - Genomics 2023Quote: The purified cDNA was PCR amplified for 6 cycles to generate dsDNA with NEBNext Ultra II Q5 High-Fidelity 2X Master Mix (NEB, M0544) and 0.5 uM PCR primers with unique dual index using the following PCR cycles:
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High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... cDNA was synthesized from 6 µL of eluted RNA using the ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs, E6300) with oligo-dT primers ...
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis was performed to generate the AeCRKG359E (inactive kinase mutation) and AeRLCK2G110E (rlck2-6 mutant allele mutation) variants using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The primers used were AeCRKkin_Mut_G359E_F and AeCRKkin_Mut_G359E_R and the AeRLCK2mutL42F and AeRLCKmutL42R ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was collected from parental iOvCa147 cells and DYRK1A-/-cells from 24-hour adherent or 6-hour spheroid conditions using the Monarch Total RNA Miniprep Kit (NEB #T2010S) as described above ...
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bioRxiv - Neuroscience 2024Quote: ... was added at 1:6 concentration to each sample and the Quick-Load® Purple 100 bp DNA Ladder (#N0551, NEB) was used as a size indicator ...
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bioRxiv - Molecular Biology 2024Quote: ... Oligo pools were cloned into the vector at a 6:1 molar ratio by Gibson Assembly at 50°C for 30 minutes (NEB E2611). The plasmid pools were ethanol precipitated ...
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bioRxiv - Biochemistry 2024Quote: ... 1 – 30 µL of cDNA was amplified by 6 to 12 PCR cycles using Illumina indexing primers and Q5 polymerase (New England Biolabs, M0491). For a typical miRXplore miRNA library from 500 ng input ...
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bioRxiv - Biophysics 2024Quote: The 2N4R isoform of 6×His-tagged human tau protein was cloned into the pRK172 vector and expressed in LEMO21 cells (NEB #C2528J). Cells were cultured in TB media at 37 °C until the OD600 reached 0.6 ...
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bioRxiv - Developmental Biology 2024Quote: Cells at passage 35-60 were harvested as a single cell suspension using TrypLE Express and 800K cells were nucleofected in 100 μl final volume containing either 2 μg of the Cas9 nickase expression vector 47 with 1 μg of each of the two sgRNAs and 10 μg of the targeting vector (for the INS eGFP allele) or 6 μl Cas9-NLS protein (120 pmol, NEB, M0646M) with 2 μg of each of the sgRNAs (Table S2 ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted from approximately 6 million COLO320-DM cells using the Monarch HMW DNA Extraction Kit for Tissue (NEB #T3060L) following the Oxford Nanopore Ultra-Long DNA Sequencing Kit V14 protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Whole-genome data for these latter strains was obtained by extracting genomic DNA from 6 pooled adult aphids using a Monarch® Genomic DNA Purification Kit (New England BioLabs), one pool per strain ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acid (5 μL) was used as template for reverse transcription using LunaScript® RT SuperMix Kit (New England Biolabs, Hitchin, UK) in 20 μL reaction volume ...