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Citations for New England Biolabs :
501 - 550 of 2049 citations for 6 Benzyloxy 1H indole 2 boronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Amino acids substitutions in ompT were generated using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... Plasmid region corresponds to the 71-1184 amino acids of BimC and the modified vector was amplified and assembled with KLD Enzyme Mix Reaction (NEB) to generate the recombinant BimC(Δ1-70)-GFP construct ...
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bioRxiv - Cell Biology 2024Quote: ... was inserted into murine Shh between amino acids 92N and 93T (corresponding to N91 and T92 in human Shh) by using Gibson assembly (HiFi Assembly Kit, NEB). Where indicated ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of RNA was assessed by comparing the known nucleic acid concentrations of the DNA ladder bands (NEB, N3232L). Microinjection protocol was followed as mentioned previously 19 using Nanoject II injector (Drummond Scientific Company ...
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bioRxiv - Plant Biology 2024Quote: ... Aspartic acid 1209 of MBD9 was mutated to Alanine through GAT→GCT DNA sequence change using the New England Biolabs (NEB) Site Directed Mutagenesis Kit and D1209A Mutagenic primers listed in Supplemental Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... the DNA sequence coding for amino acids 384 to 667 of Fbxo42 was cloned into pETM-30 at XhoI/NcoI (NEB) restriction sites ...
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bioRxiv - Cell Biology 2024Quote: ... a PCR product containing the sequence encoding amino acids 104-330 of MPS1 from Drosophila melanogaster was inserted into the pMal-c2 vector (New England Biolabs) by FastCloning (Li et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... The signal peptide was removed (amino acids 1-35) by mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: ... A truncated version of this wild-type VgrS lacking the first 81 amino acids was created by Q5-mutagenesis (NewEngland Biolabs, NEB) to generate VgrS (residues 81-729 ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg RNA were incubated for 30 minutes at 37°C with 2 μl RNA 5’ Pyrophosphohydrolase (NEB, M0356, 5,000 units/ml). The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO ...
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bioRxiv - Molecular Biology 2024Quote: ... attB plasmid containing genes for tdMCP-protein fusions and a MS2-circRNA barcode were digested overnight at 37°C to remove existing barcode sequence (2 μg plasmid, 5 μL 10x CutSmart buffer, 2 μL BsrGI-HF (NEB cat#R3575S), nuclease-free water to 50uL) ...
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bioRxiv - Molecular Biology 2024Quote: ... was digested by combining the following and incubating overnight at 37°C: 2 μg plasmid + 5 μL CutSmart buffer (10x) + 2 μL AflII (NEB cat# R0520S) + 2 μL BlpI (NEB cat# R0585S ...
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bioRxiv - Bioengineering 2024Quote: ... Gibson assembly was performed by combining a part amplicon and linearized pEmpty in a 2:1 mass ratio in 2× NEB Gibson Assembly Master Mix (NEB, Cat# E2611S) and incubating at 50℃ for 2 hr before transformation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2 uL of T4 RNA ligase I (NEB M0204S) totaling 20 uL and incubated for 1 hour at 30 C.
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10X RtcB reaction buffer (New England Biolabs), 2 μl 1mM GTP ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by adding 2 μl of RNase A (NEB) and incubating at 37 °C for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1x T4 RNA Ligase 2 reaction buffer (NEB, M0239S), and 0.5 units/µl T4 RNA Ligase 2 (NEB ...
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bioRxiv - Genomics 2020Quote: ... the NEBNext High-Fidelity 2× PCR Master Mix (NEB) was used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 5 ml 1x NEB Buffer 2 (NEB) and drop frozen in liquid nitrogen ...
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bioRxiv - Genomics 2020Quote: ... 1 µl T4 RNA Ligase 2 truncated (200U; NEB)] was added ...
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext HiFi 2× PCR Master mix (NEB) was added ...
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bioRxiv - Bioengineering 2021Quote: ... 2 unit of murine RNase inhibitor (New England Biolabs), varying amounts of target RNA ...
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bioRxiv - Bioengineering 2021Quote: ... Its constituents were 1X RNA Ligase 2 buffer (NEB) supplemented with 5% PEG 8000 ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... packed with 2 ml amylose resin (New England Biolabs) equilibrated with buffer AF ...
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bioRxiv - Genomics 2022Quote: ... 0.7 µl T4 ligase (2 M U/ml, NEB), 250 ng DNA (adapters in File S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 units of phi29 DNA polymerase (New England Biolabs), three not to quantified deoxynucleoside triphosphate mix (20 μM) ...
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bioRxiv - Microbiology 2022Quote: ... 20 μl Phusion DNA polymerase (2 U/μl, NEB), 160 μl Taq DNA ligase (40 U/μl ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of undiluted Thermolabile Proteinase K (NEB #P8111S) and 1 μl SUPERaseIN (20 U/μl ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μl of 10 mM dNTPs (New England Biolabs), 2.5 μl of 10 μM oligonucleotide A (unmodified forward primer ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... for 2 hours or ShortCut RNaseIII (New England Biolabs) for 20 minutes at 37°C after permeabilization before blocking.
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl Thermostable 5’App ligase (New England Biolabs), 4 μl 10 μM pre-adenylated R1R Adapter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.5 vol 2×Phusion Master Mix (New England Biolabs), and 0.04 vol P1 and P2 amplification primers (10 nm) ...
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bioRxiv - Genomics 2021Quote: ... 2uL 2 000 000U T4 DNA ligase (NEB, M0202). The reaction was incubated at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... disulfide bonding enhancer 1 and 2 (New England BioLabs), RNase inhibitor (Takara Bio Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... 1X Phusion buffer and 2 U Phusion Polymerase (NEB). The PCR protocol used was an initial denaturation of 30 s at 98 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL of 10% NP-40 (NEB Cat # B0701S) and 6 μL of water were mixed with the 10 μL of denatured spike glycoprotein ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μl NEBuffer 2 (New England Biolabs, Ipswich, USA); 0.3 μl T7E1 enzyme at 10 Units/μl (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... or 2 mU DNase 1 (Cat. No. M0303S; NEB) and 0 ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μL NEBNext HiFi 2 × PCR Master mix (NEB) was added ...
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bioRxiv - Genomics 2022Quote: ... 2 µL 400U/µL T4 DNA ligase (NEB, #M0202V), 10 µL 10mM ATP (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 mM MnCl2 (New England Biolabs, NEB). Control treated cells were incubated with a volume of 50% glycerol equivalent to the volume of RNase III added as well as 2 mM MnCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 mM MnCl2 (New England Biolabs, NEB). Control treated cells were incubated with a volume of 50% glycerol equivalent to the volume of RNase III added as well as 2 mM MnCl2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2 µl of 10X ExoI buffer (NEB, B0293S). The annealing was performed in a thermocycler by gradually decreasing the temperature from 98 °C to 60 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... BamHI (Site 2) or HindIII (Control; New England Biolabs) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 U T4 RNA ligase 2 (truncated KQ, NEB) and 40 U Murine RNase Inhibitor (NEB ...
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bioRxiv - Genomics 2024Quote: ... 10 µl of 2% BSA (NEB, B9000S, 0.01% final) and 930 µl of nuclease-free water and supplemented with 1U/ml Protector RNase inhibitor (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 6,25µl of LongAmp Taq 2✕ Master Mix (NEB), 4,25µl of milliQ water ...
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bioRxiv - Bioengineering 2023Quote: ... 2 µL of PNGase F (New England Biolabs, MA) was added ...