Labshake search
Citations for New England Biolabs :
7301 - 7350 of 10000+ citations for Human Oxidized low density lipoprotein receptor 1 OLR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The RNA-Seq library was prepared by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center) ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA was isolated according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit; #T2010S New England Biolabs). As a quality control ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were converted to library using NEBNext® Small RNA Library kit (ref#E7330S, New England Biolabs, UK) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... prior to library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) with poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP samples were analyzed by qPCR or subjected to library preparation according to the manufacturer’s protocol (NEBNext Ultra II DNA Library Prep Kit for Illumina, NEB) using Dual Index Primers (NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... the 5S and Saba PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB, # T1030). The purified DNA sequence was then analyzed through Sanger sequencing (GENEWIZ from Azenta ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro transcription was performed using HiScribe™ T7 or SP6 High Yield RNA Synthesis Kit (New England Biolabs). RNA was subsequently purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Cancer Biology 2022Quote: ... mRNA was isolated from 1.5 μg of total RNA using the kit NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). NGS libraries were prepared from the purified mRNA using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... The product bend was cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB). 32P-GTP labelled U1 RNA was prepared by in vitro transcription as described previously with addition of 0.5 μL of α-32P-GTP (3.3 μM ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were directly subjected to qRT-PCR using Luna Universal One-Step RT-qPCR Kit (NEB, #E3005L). The primer sets used in qRT-PCR to detect CI-MPR and CD-MPR mRNA are 5’-CATTCAGTGGGTGACTCTGTT-3’/5’-TGCTCTGGACTCTGTGATTTG-3’ and 5’-GCTCTAGTGAAGAGGCTGAAAC-3’/ 5’-GCACACCCTGAAGATGTAGATG-3’ ...
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bioRxiv - Biophysics 2022Quote: ... The 8C-8A(IL125) chimera cDNA construct was generated using the Phusion High-Fidelity PCR kit (New England BioLabs). All cDNAs were tagged on their carboxy terminus with Myc-DDK epitopes ...
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bioRxiv - Developmental Biology 2022Quote: ... Library preparation was performed with the NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB, Cat #E7645S) using 25 μL of CUT&RUN DNA as input ...
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bioRxiv - Cell Biology 2022Quote: ... The amplicons (200 fmol) were first end-repaired with the NEBNext Ultra II End repair kit (New England Biolabs), then purified using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... ICL3 and S305A mutations were generated using back-to-back primers on pCDNA3.1-HCAR1-Flag vector by Q5 Site-Directed Mutagenesis Kit (NEB). Fluorogen activating peptide fusion to HCAR1 was synthesized with insertion of HCAR1 using BsmI site into pMFAP-β1 vector (Spectragenetics) ...
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bioRxiv - Cell Biology 2022Quote: ... Purified DNA was cloned into pAV0714 (Vještica et al., 2020) using NEBuilder® HiFi DNA assembly kit (NEB #E2621). After being verified by Sanger sequencing ...
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bioRxiv - Genomics 2022Quote: ... ChIP samples were processed for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instruction and sequenced on a Next-Seq 500 Sequencer (Illumina ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using NEB Next® Ultra™ RNA Library Prep Kit (NEB, San Diego, CA, USA), employing AMPure XP Beads to purify the products of the reactions during the library prep ...
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bioRxiv - Genetics 2022Quote: Libraries were generated with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). mRNA was enriched from total RNA (250 ng ...
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bioRxiv - Genetics 2022Quote: All of the plasmids created by Gibson assembly used the NEBuilder HiFi DNA Assembly kit (New England Biolabs, #E2621). To construct the URA3 RRM3 plasmid ...
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bioRxiv - Genomics 2022Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The purified UVRh1 amplicons were cloned into the minT vector using the PCR cloning kit and following the manufacturer’s protocol (NEB). The cloned amplicons were sequenced by Retrogen Inc ...
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bioRxiv - Plant Biology 2022Quote: ... RNA samples were used to generate sequence libraries using a library prep kit from New England Biolabs (NEB #E7530). Paired-end sequence reads of 75 bp were generated at the TUFTs genomic center at the Tufts University School of Medicine using the Hi-Seq Illumina platform ...
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bioRxiv - Microbiology 2022Quote: ... the start codon of each uORF was mutated using a Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The sequence additions and mutations of all plasmids were confirmed by DNA sequencing (Source Bioscience).
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were constructed using NEB Next® UltraTM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2022Quote: ... RNA Libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced as single end reads using the NextSeq500 platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to the NEBNext Ultra RNA II directional Library Prep kit (New England Biolabs, MA, USA) after NEBNext rRNA depletion (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation was performed with the NEBNext Ultra DNA Library prep kit according to the manufacturer’s instructions (NEB, E7370L). 4C-seq libraries were sequenced on an Illumina Hiseq2500 platform (50 cycles ...
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bioRxiv - Immunology 2022Quote: ... Library quality and quantity were assessed by qPCR method using NEB Next Library Quant Kit for Illumina (NEB, # E7630) and Qubit ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA of the gram-negative bacteria was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs). After isolation of the genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... for removal of genomic DNA before it was purified using the Monarch RNA cleanup kit (New England Biolabs, USA). The RNA and potential DNA contamination concentrations were quantified using Qubit RNA BR and Qubit dsDNA HS assay kits ...
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bioRxiv - Microbiology 2022Quote: ... ShdA III and ShdA IV homologues were synthesised in vitro using the PURExpress cell-free transcription/translation kit (NEB), from PCR products ...
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bioRxiv - Microbiology 2022Quote: ... and libraries were quantitated with qPCR (Bio-rad CFX96 Touch Real-Time PCR, NEB Library Quant Kit for Illumina). Libraries were normalized to 0.5 nM and pooled ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Antisense strand DNA was digested in the samples using the Uracil-Specific Excision Reagent Enzyme Kit (New England Biolabs), followed by a PCR to amplify the cDNA ...
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bioRxiv - Plant Biology 2022Quote: ... The small RNA libraries were prepared using the NEBNext Small RNA Sample Prep kit (New England Biolabs, Ipswich, MA). High throughput sequencing was performed with NovaSeq-6000 (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420). RNA libraries were sequenced on an Illumina NovaSeq 6000 platform (PE150 bp) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting plasmid backbone of the linearized vector was run on the 0.8% agarose gel and purified by Monarch DNA Gel Extraction Kit (NEB). The oligos for the inserted sgRNA targeting sequences were synthesized with two overhangs compatible with the linearized vector and complementary to each other ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® was used (New England Biolabs, MA, USA). For sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... DNA fragments were inserted into plasmid vectors using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Massachusetts, USA). The primers used in this study are listed in the Supplemental Table S9 ...
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bioRxiv - Molecular Biology 2023Quote: The following M35 derivative constructs were derived from pcDNA3.1 TOPO M35-V5/His using the Q5 site-directed mutagenesis kit (#E0554, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The inserts and backbone were assembled using the NEB HiFi DNA assembly kit (New England Biolabs, Ipswich, Massachusetts, USA), resulting in the final plasmids ...
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bioRxiv - Microbiology 2024Quote: ... primers oAA033-034 were used to remove mprF using the Q5 site-directed mutagenesis kit (NEB Cat. No. E0554S) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using dual-end indexing using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645L) using the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs) according to manufacturer’s instructions in combination with a poly(A ...
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bioRxiv - Cancer Biology 2024Quote: ... Mutations and insertions were introduced by site directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs). Oligonucleotides for site-directed mutagenesis were designed using the NEBaseChanger version 1.3.3 (New England Biolabs ...
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bioRxiv - Physiology 2022Quote: ... cDNA was prepared following the NEBNext® Ultra™ II Directional RNA Library Prep Kit protocol (New England Biolabs). The libraries were sequenced using paired-end 150 bp sequencing on a HiSeq 4000 System (Illumina) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with a primer/probeset binding in the orf1ab region (forward ...
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bioRxiv - Molecular Biology 2022Quote: ... and in vitro transcription was performed using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA, USA) to generate IVT mRNA according to the manufacturer’s instructions ...