Labshake search
Citations for New England Biolabs :
7351 - 7400 of 10000+ citations for Human Oxidized low density lipoprotein receptor 1 OLR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using the manufacturer’s instructions (New England Biolabs). Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by T7 in vitro transcription using the HiScribe T7 In Vitro Transcription Kit (New England Biolabs, Ipswich, MA) and purification with Trizol (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... and ChIP libraries were amplified and barcoded using the NEBNext Ultra II DNA library preparation kit (New England Biolabs) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2022Quote: ... FLAG-tagged ORF3c mutant Q5Y was prepared by site-directed mutagenesis using a commercial kit (New England Biolabs, E0554S).
-
Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, #E7103) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: Deglycosylation of total brain homogenates was performed according to the PNGase F kit instruction manual (#P0704S, New England Biolabs). 40µg of protein were digested with 1,000 U of PNGase F enzyme.
-
bioRxiv - Biophysics 2024Quote: ... The cDNAs were transcribed in vitro to cRNAs using the HiScribe® T7 Arca mRNA Kit (New England BioLabs). Single mutations in Cx26 (W3A ...
-
bioRxiv - Systems Biology 2024Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... In vitro transcription (IVT) eactions were performed with HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs). Template DNA was degraded with TURBO DNase (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2024Quote: ... and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB, Ornat, Rehovot, Israel, #E7770L). Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes ...
-
bioRxiv - Developmental Biology 2023Quote: ... The gRNA was made by in vitro transcription using a Hiscribe T7 high yield kit (New England Biolabs #E2040s) using the following primers (Integrated DNA Technologies) ...
-
bioRxiv - Plant Biology 2024Quote: ... In-frame deletions and substitutions were generated using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Hitchen UK) using the primers listed in Table S1 ...
-
bioRxiv - Plant Biology 2024Quote: ... The Illumina cDNA library was prepared using a NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA, USA) according to the standard experimental protocol ...
-
bioRxiv - Plant Biology 2024Quote: ... Adapter ligation and library preparation were carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), and adapter ligation was checked using qPCR ...
-
Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... Library preparation was carried using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103). Sequencing was performed on an Illumina HiSeq4A to produce paired-end reds of 125 bp ...
-
bioRxiv - Microbiology 2024Quote: ... Metagenomic sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs, MA, USA) with a targeted insert size of 350-400bp and Dual Index multiplex oligos ...
-
bioRxiv - Neuroscience 2024Quote: ... Illumina library was made from polyA RNA with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s manual74 ...
-
bioRxiv - Zoology 2024Quote: ... Total RNA was then extracted using a Monarch® Total RNA Miniprep Kit (New England Biolabs, Whitby, ON, Canada) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... Purified RNA was depleted of ribosomal RNA (rRNA) using a NEBNext® rRNA Depletion Kit (Bacteria) (NEB, #E7850L/X) and library preparation was done using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
-
bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis transgenic plants were generated by using the Q5 ® Site-Directed Mutagenesis Kit (New England Biolabs) in the pDONR221 vector and recombined with the above-mentioned promotor constructs into the pFR7m24GW vector using the Gateway Technology ...
-
bioRxiv - Synthetic Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA library Prep Kit for Illumina per manufacturer’s instructions (New England Biolabs). mRNAs were enriched with Oligo(dT ...
-
bioRxiv - Molecular Biology 2023Quote: ... pLenti-PGK-ATP6V1H(Δ176-191)-FLAG-Puro was produced using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). M4P-EGFP-LC3B and pOGP were kind gifts from F ...
-
bioRxiv - Physiology 2024Quote: ... Libraries were constructed from polyA-selected RNA using a NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) and sequenced on Illumina HiSeq2500 instrument ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs), validated on TapeStation (Agilent Technologies) ...
-
bioRxiv - Microbiology 2024Quote: ... PCR products were then either purified directly using Monarch PCR and DNA Cleanup Kit (New England Biolabs, Hitchin, UK), or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... an equimolar pool of all amplicons was prepared and purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). After Qubit quantification using Qubit® dsDNA HS Assay Kit and Qubit 2.0 fluorometer (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by the GFP sequence were generated by using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, E2060). 1 µg mRNA was first denatured at 65°C for 3 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared as in [34] with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).
-
bioRxiv - Neuroscience 2024Quote: ... Mutations of PICK1 (KD-AA and 5K-E) were made with Q5® Site-Directed Mutagenesis Kit (NEB #E0554S). The constructs were purified by NucleoBond® Xtra Midi EF (MACHEREY-NAGEL) ...
-
bioRxiv - Immunology 2024Quote: ... mRNA was purified from 4 µg of total RNA with a magnetic mRNA isolation kit (New England Biolabs, S1550S). cDNA was synthesized from mRNA samples with M-MLV (New England Biolabs ...
-
bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were then constructed using the NEBNext Ultra II RNA Library prep for Illumina kit (NEB#E7760L) NEBNext Poly(A ...
-
bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were constructed using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) using ChIP products from “OSC ChIP” and “Ovary ChIP” ...
-
bioRxiv - Neuroscience 2024Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) and subsequently sequenced by Illumina NovaSeq 6000 or Illumina NextSeq 500/550 (LabGenomics ...
-
bioRxiv - Microbiology 2024Quote: ... The resulting RNA (300 ng) was depleted of ribosomal RNA using the NEBNext rRNA Depletion Kit (NEB Cat#E6310). rRNA-depleted RNA was purified using RNAClean XP beads (Beckman-Coulter Cat#66514) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Library preparation was prepared after polyA mRNA selection using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). Sequencing was performed on Illumina NovSeq600 using a 2×150 paired end configuration ...
-
bioRxiv - Microbiology 2024Quote: ... 25 °C) using a custom methionine(-) Flexible In vitro Translation system composed by PURExpressTM (ΔRF123) Kit (New England Biolabs) solution B ...
-
bioRxiv - Immunology 2024Quote: ... RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes ...
-
bioRxiv - Biochemistry 2024Quote: ... The activating Q163C/Q309C cysteine mutations were introduced into αMI-domain using the Q5 Site-Directed Mutagenesis Kit (NEB). The plasmids were transformed into either BL21(DE3 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... in conjunction with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760) and the NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs ...
-
bioRxiv - Neuroscience 2024Quote: ... The libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA), and the preparation involved a polyA selection method ...
-
bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs) according to manufacturer’s instructions in combination with a poly(A ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mutations and insertions were introduced by site directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs). Oligonucleotides for site-directed mutagenesis were designed using the NEBaseChanger version 1.3.3 (New England Biolabs ...
-
bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using dual-end indexing using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645L) using the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs, USA). 4 μL of cDNA was added to 1 μL of pre-purchased primers (QuantiTect primers (Qiagen ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA libraries were prepared using an established protocol with NEBNExt Ultra II Directional Library Prep Kit (NEB, Lynn, MA). Each library was made with one of the TruSeq barcode index sequences and the Illumina sequencing done by Medgenome (Foster City ...
-
bioRxiv - Molecular Biology 2023Quote: Isotope labeled firefly luciferase (Fluc) mRNA was synthesized by HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB, Ipswich) using stable heavy isotopes of ATP and CTP (NLM-3987-CA and CNLM-4267-CA-20 ...
-
bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
-
bioRxiv - Biophysics 2024Quote: ... and HCN4 Δ1-185 deletion clones were made using a site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) and a codon-optimized HCN4 plasmid in the pTwist-CMV-WPRE-Neo vector synthesized by Twist Biosciences.
-
bioRxiv - Immunology 2024Quote: Libraries for sequencing were prepared using extracted RNA and the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (New England Biolabs). Quality of all RNA and library preparations were evaluated with BioAnalyzer 2100 (Agilent).
-
bioRxiv - Plant Biology 2023Quote: ... In vitro transcription was performed using the HiScribe T7 ARCA mRNA (with tailing) kit (New England Biolab, http://www.NEB.com) and Xenopus oocytes were injected with 30 ng of RiSKC3 cRNA using a pneumatic injector ...