Labshake search
Citations for New England Biolabs :
651 - 700 of 3540 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... resuspended in 400 μl of TM2 buffer containing micrococcal nuclease (New England Biolabs) and 1mM CaCl2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 39 µL of qPCR mix containing 1× Phusion mix (New England Biolabs, M0531S), 0.125 µM P6 Tall v4 primer ...
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... digested in buffer containing 10 mM DTT with Proteinase K (New England Biolabs), and expanded in ultrapure water as for the cell work ...
-
bioRxiv - Biochemistry 2022Quote: ... Amplifications were performed in 100 µl reactions containing 1X Phusion HF buffer (NEB), 1 µM of each primer (ordered from IDT) ...
-
bioRxiv - Biochemistry 2022Quote: ... Cell lysates were applied to a column containing amylose resin (New England Biolabs). The beads were washed with at least ten column volumes of buffer containing 50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.05% Tween-20) containing 10 µMnt of circular ssDNA (PhiX174 virion DNA, NEB) immobilised on magnetic Streptavidin-coated beads (Dynabeads M-280 ...
-
bioRxiv - Biochemistry 2023Quote: ... 4 U/mL creatine kinase) containing 10 µMnt cssDNA (NEB, PhiX virion DNA) was supplemented with Rad51 (5 µM ...
-
bioRxiv - Bioengineering 2023Quote: ... Amplifications were performed in 100 µl reactions containing 1X Phusion HF buffer (NEB), 1 µM of each primer ...
-
bioRxiv - Biophysics 2023Quote: ... coli (MG1655) genome containing the rrnC operon was amplified by PCR (Phusion, NEB) using the following primers:
-
bioRxiv - Genetics 2024Quote: ... mRNA was hydrolysed in a buffer containing 10U of nuclease P1 (NEB, M0660S), NaCl and ZnCl2 for 2 hours with agitation at 800 rpm for 30s every 5 min at 37°C in thermomixer (Thermofisher) ...
-
bioRxiv - Genomics 2024Quote: ... a 750 μl solution containing 1x NEB T4 DNA ligase buffer (NEB B0202), 120 μg of BSA (ThermoFisher AM2616) ...
-
bioRxiv - Microbiology 2024Quote: ... the previous large plasmid containing 1x parS site was digested with NruI (NEB) and dephosphorylated ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatant was added to a column containing 2.5 mL amylose resin (NEB). The resin was washed with 50 mL of column buffer and proteins were eluted with 20 mL cold Column buffer containing 10mM maltose ...
-
bioRxiv - Genetics 2024Quote: ... a 10 µL reaction containing 0.2 µL AseI (New England BioLabs, MA, USA) and 1 µL CutSmartTM r3.1 Buffer (New England BioLabs ...
-
bioRxiv - Developmental Biology 2024Quote: ... a construct containing the following DNA elements was assembled by HiFi cloning (NEB): (1 ...
-
bioRxiv - Biochemistry 2024Quote: ... containing 1x Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs), 0.5 µM of the forward and reverse primer ...
-
bioRxiv - Cell Biology 2022Quote: ... and protein samples and molecular weight markers (PageRuler Plus Pre-stained Protein Ladder, ThermoFisher Scientific 26619, or Colour pre-stained protein standards NEB P7712 or P7719) were separated using 8% ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 % SDS-PAGE gel was prepared and 50 μg of protein lysate was loaded along with the protein standard ladder (Cat.No.: P7719S, New England Biolabs, Ipswich, Massachusetts, United States), and electrophoresis was performed for 1.5 hr in ice-cold 1x Laemmli electrophoresis running buffer ...
-
bioRxiv - Immunology 2024Quote: ... Strand extension was performed at 37°C for 10 minutes using 5uM of ssDNA binding protein (T4 Gene 32 Protein, NEB, Cat No. M0300S) and 0.8U/µL of Klenow Fragment (3’-5’ exo- ...
-
bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
-
bioRxiv - Biophysics 2020Quote: For the insertion of an ATTO647N-labeled oligonucleotide complementary to the position 14711 bp from the biotinylated end of the λ DNA we employed the previously described strategy14 and followed the more recently described procedure.15 2 μg of λ DNA was incubated for 2 hours with the nicking enzyme Nt.BstNBI (20 units, NEB) at 50 °C in the nickase buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... PCR product (2 μg) and pEYFP-C1 vector DNA (2 μg) were digested with BamHI-HF and HindIII-HF (New England Biolabs) according to the manufacturer ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The annealing was carried out in 96-well PCR plates by mixing 2 μL of each oligonucleotide at 100 μM with 2 μL of 10x T4 DNA Ligase Reaction Buffer (NEB) and 14 μL of water ...
-
bioRxiv - Systems Biology 2021Quote: ... PCR reactions with plasmid and genomic DNA templates were performed using the Phusion High-Fidelity 2× Master Mix or Q5 High-Fidelity 2× Master Mix (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2021Quote: ... was amplified and barcoded in parallel using the same PCR program in 50 μL PCR reaction (2 μL gap-repaired DNA, 25 μL 2× NEBNext High-Fidelity PCR Master Mix (NEB), 1.5 μL 10 μM i5 universal PCR primer ...
-
bioRxiv - Synthetic Biology 2023Quote: ... we first mixed 10.5 uL of purified RNA with 2 uL of 2 uM RT primer (AGGGACATCGTAGAGAGTCGTACTTANNNNNNNNNNAGATGAACTTCAGGGTCAGC, where Ns comprise the UMI) and 2uL of 10mM dNTP mixture (NEB), incubated the mixture at 65C for 5 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... and ligated to hairpin adaptor 2 (ENDseq-adaptor-2, 5′-Phos-GATCGGAAGAGCACACGTCUUUUUUUUAGACGTGTGCTCTTCCGATC*T-3′ [*phosphorothioate bond]) (Quick ligation kit, NEB). To prepare libraries for sequencing ...
-
bioRxiv - Microbiology 2022Quote: ... human NINL isoform 2 (NCBI accession NM_001318226.2) and the NINL isoform 2 mutant (Q231R) were mutagenized using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The plasmids encoding 3C proteases (coxsackievirus B3 (CVB3 ...
-
bioRxiv - Cell Biology 2023Quote: ... A repair template for editing endogenous plk-2 gene to express PLK-2::3xFLAG::TIR1CIP was assembled into a pCR-Blunt backbone using Gibson Assembly (NEB) and confirmed using Sanger sequencing and whole-plasmid Nanopore sequencing (Primordium) ...
-
bioRxiv - Microbiology 2024Quote: ... 5’ pir-2 flank::PtrpC::nat::3’ pir-2 flank assembly was constructed using NEBuilder Assembly Kit (New England Biolabs). Each assembly fragment was either synthesized (T4 Oligo ...
-
bioRxiv - Molecular Biology 2021Quote: ... 41.7 µL of Phusion 2× Master Mix (NEB), 2.5 µL of 10 µM primer mix (515F forward and 806R reverse rRNA gene V4 primers with Illumina MiSeq adaptors) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... treated with 2 units of DNase I (NEB) at 37°C for 1 hour followed by DNase I heat inactivation ...
-
bioRxiv - Genomics 2020Quote: ... 2 μl Antarctic Phosphatase [5k U/mL] (NEB) and 1 μl RNase inhibitor and incubating at 37 °C for 30 minutes with shaking ...
-
bioRxiv - Genomics 2021Quote: ... 10 µL of 2% BSA (New England Biolabs), 930 µL of nuclease-free water and supplemented with 0.2 U/ul RNaseIN Plus (Promega ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 µl RNase Inhibitor (80U final, NEB M0307L) and 2 µl SuperScript III (400U final ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 µl RNase Inhibitor (80U final, NEB M0307L) and 1 µl AMV RT (12U final ...
-
bioRxiv - Genetics 2021Quote: ... viewpoint 2: 10X NEBuffer™ DpnII (R0543M, NEB)) and 15 μl of 10% SDS buffer were added to the 450 μl sample ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 2 μl 10x CutSmart buffer (New England Biolabs), 10 μl shrimp alkaline phosphatase (New England Biolabs) ...
-
bioRxiv - Microbiology 2021Quote: ... 2 μl 10× T4 RNA ligase buffer (NEB), 20 U T4 RNA ligase enzyme ...
-
bioRxiv - Microbiology 2021Quote: ... 2 μl of 10× Antarctic Phosphatase buffer (NEB), 1 U Antarctic Phosphatase ...
-
bioRxiv - Neuroscience 2022Quote: ... and 2 µl T4 ligase (NEB cat #M0202M). For Capture-C ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 2 μl T4 Ligase Buffer (NEB, B0202) in a total volume of 30 μl for 18 hours at 16 °C ...
-
bioRxiv - Immunology 2022Quote: ... dNTP mix (2 mM each) (New England Biolabs), 50U Superscript III RTase ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for 30 min ...
-
bioRxiv - Plant Biology 2021Quote: ... M-TM2^2 was digested with BslI (NEB) for 3 hours at 55°C and M-2 was digested with XhoI (NEB ...
-
bioRxiv - Biophysics 2021Quote: ... 2 nM M13mp18 circular ssDNA (New England Biolabs) (Table S3 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... added Cut smart (2 ul, Cat # B7204; NEB), Bsa I (2ul ...
-
bioRxiv - Microbiology 2022Quote: ... 2 μl of RNaseH (10 U, NEB, M0297) was added followed by an incubation of 20 minutes at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 μl 10x NEBuffer 1 (New England Biolabs), 2 μl 50 mM MnCl2 ...
-
bioRxiv - Microbiology 2021Quote: ... and Vaccinia 2’-O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...