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Citations for New England Biolabs :
601 - 650 of 3540 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The gel-extracted nascent RNA in 5 µl nuclease-free water was ligated to 10.7 pmol barcode DNA linker (Supplementary Table 2) using 200 U truncated T4 RNA ligase 2 (NEB) overnight at 16°C ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were expressed in BL21 DE3 (New England Biolabs) at 37 °C and 180 rpm after Isipropyl-thiogalactoside (IPTG ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates were additionally dephosphorylated using Lambda protein phosphatase (NEB) following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Then 1.5 ul of 50 μM LbaCas12a protein (NEB) or 1 ul of 63 μM AsCas12a Ultra protein (IDT ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then further purified over amylose resin (NEB) and eluted with elution buffer (20 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... The donor DNA (0.6μM) with Cas9 protein (NEB #M0646T), crRNA and tracrRNA were injected in a 1:1:1:1 molar ratio into mouse zygotes by the UCSD Transgenic and Knockout Mouse Core (See Supplementary Table 4 for crRNA sequence) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were generated using cell lysis buffer (NEB) and brief sonication on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... We digested the proteins with Proteinase K (NEB #P8107S) for 5 min at 50 °C ...
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bioRxiv - Pathology 2021Quote: ... and 0.4 μg of T4 gene protein (NEB, M0300S)] ...
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bioRxiv - Genomics 2021Quote: ... The purified recombinant protein or commercial M.EcoGII (NEB, M0603S) was diluted with coating buffer (0.05 M NaHCO3 buffer ...
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bioRxiv - Microbiology 2021Quote: ... 9 Neuraminidase A (P0722, NEB, 4 units/µg protein). 10 µg glycoprotein ...
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bioRxiv - Microbiology 2020Quote: ... anti-maltose binding protein (MPB) (New England BioLabs #E8032L) and anti-outer membrane protein (OmpA ...
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bioRxiv - Cell Biology 2020Quote: ... against a Color Protein Standard broad range ladder (NEB) in NuPAGE MES SDS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μL of Lambda Protein Phosphatase (New England Biolabs) or 1 μL of phosphatase inhibitor mix (20 mM β-glycerophosphate ...
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bioRxiv - Developmental Biology 2021Quote: ... A pre-assembled complex of purified Cas9 protein (NEB) and gRNA was injected and the efficiency of Crispr/Cas9-induced mutagenesis in the F0 generation monitored at 24 hpf using a T7 endonuclease assay (Jao et al. ...
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bioRxiv - Microbiology 2020Quote: ... RNA-protein complexes were dephosphorylated with T4 PNK (NEB) for 45 minutes in an Eppendorf Thermomixer at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μM of purified proteins mixed with PEG8000 (NEB) at 10% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... MBP-MYB6 protein was purified on amylose resin (NEB) and HIS-PUB26 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix ...
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bioRxiv - Synthetic Biology 2020Quote: The protein samples of purified ribosome (New England BioLabs), E ...
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bioRxiv - Genetics 2020Quote: ... 90 nM of SpCas9 protein (#M0386, New England Biolabs), NEB buffer 3.1 ...
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bioRxiv - Neuroscience 2020Quote: ... H1 was transfected with pre-assembled Cas9 protein (NEB) + crRNA and tracrRNA (Synthego) ...
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bioRxiv - Immunology 2021Quote: ... 90 nM of EnGen® Sau Cas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Genetics 2020Quote: ... MEFs were protein transfected with recombinant RNase H (NEB) using Project Reagent Transfection Kit (Thermo ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were used with lambda protein phosphatase (NEB) treatment according to its protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... using a commercially available Cas9 protein (New England Biolabs). The efficiency of creating deletion after co-injection of the sgRNA pair was determined by PCR on genomic DNA extracted from injected embryos using the following primers:
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bioRxiv - Immunology 2023Quote: ... color prestained protein standard (New England Biolabs GmbH, Germany) was included on each gel ...
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bioRxiv - Bioengineering 2023Quote: ... Tagless protein was expressed in Lemo21(DE3) cells (NEB) in LB (10 g Tryptone ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions (Lambda protein phosphatase, Biolabs). Western blot analysis was followed with an antibody against GFP (1:1000 ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions (Lambda protein phosphatase, Biolabs). In parallel ...
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bioRxiv - Cell Biology 2024Quote: ... RRID:Addgene_119754).CAPS-GST protein was expressed in BL21Star bacteria (NEB); the protein was isolated by binding to glutathione-Sepharose (Pierce ...
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bioRxiv - Neuroscience 2024Quote: ... 25-50 ul Protein G Magnetic Beads (NEB #S1430S) were incubated with 3ug GFP (D5.1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Synthesized sgRNA was mixed with Cas9 protein (NEB# M0646T) just before microinjection into zebrafish embryo.
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bioRxiv - Biophysics 2023Quote: ... Proteins were cleaved with TEV protease (New England Biolabs) and further purified via reverse IMAC ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subsequently purified on amylose resin (E8021L, NEB): 3 ml resin per column and 45 ml of extract ...
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bioRxiv - Neuroscience 2023Quote: ... Protein A beads (10 µl, New England Biolabs, #S1425) were washed twice in coIP-solution and consecutively incubated for 1 hour at 4°C with an anti-synaptopodin antibody (rabbit anti-Synaptopodin ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted protein was applied to Amylose resin (NEB) equilibrated with buffer D (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... 30 μg of Protein G magnetic beads (NEB #S1430) was washed with 250 μl of Immunoprecipitation Reaction Buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was first purified on amylose resin (NEB) in a buffer containing 20 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were separated on magnetic streptavidin resin (NEB) and eluted with 2× SDS loading buffer supplemented with 4% SDS and 10 mM Β-mercaptoethanol ...
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bioRxiv - Physiology 2024Quote: ... Nuclear proteins were extracted using nuclear extraction buffer (NEB) (20mM HEPES pH7.9 ...
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bioRxiv - Biophysics 2024Quote: ... The sample was phosphorylated using protein kinase A (NEB) and then further purified on size exclusion chromatography.
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bioRxiv - Molecular Biology 2024Quote: ... 300 pmol of Cas9 protein (NEB, Cat. No. M0386M) and 600 pmol of sgRNA were incubated in Cas9 Buffer (150 mM KCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix ...
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bioRxiv - Neuroscience 2024Quote: ... Protein deglycosylation mix II (New England Biolabs, Cat# P6044S) was added to the treated tubes and buffer only was added to the control tubes ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1% Triton X-100 and 30 Units of T4 DNA ligase (NEB), followed by ligation reaction at 16°C for 4.5 h and then at room temperature for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... containing DpnII buffer to 1x final concentration and 150 units DpnII (NEB, R0543). The digestion was incubated overnight at 37C with gentle rocking ...
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bioRxiv - Plant Biology 2020Quote: ... pGEMHE-DEST containing TaHKT1;5-D was linearized using sbfI-HF (New England Biolabs) before synthesising cRNA using the mMESSAGE mMACHINE T7 Kit (Ambion ...
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bioRxiv - Genomics 2020Quote: ... followed by addition of 20 μl Ligation master mix (containing: 15 μl NEB Quick ligation buffer ...
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bioRxiv - Microbiology 2023Quote: ... gB-Kan containing ORF31was digested by BgIII and NdeI enzymes (New England Biolabs). pCAGGS-gB plasmids containing the desired mutation were also digested with BgIII and NdeI enzymes ...