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Citations for New England Biolabs :
501 - 550 of 3540 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Each protein was digested with PNGaseF (NEB, P0705) or EndoH (NEB ...
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bioRxiv - Genetics 2024Quote: ... Ribonucleoprotein complexes of SpCas9 protein (New England Biolabs) and gRNAs were prepared with a final concentration of 200 ng/μl and 25 ng/μl for each gRNA (100 ng/μl in total) ...
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bioRxiv - Molecular Biology 2024Quote: ... together with Broad Protein Standard (New England Biolabs), and transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer system (Mixed MW transfer program ...
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bioRxiv - Neuroscience 2021Quote: ... Beads were then incubated with 100 μl reaction buffer (containing 1X NEB heparinase mix diluted in dH2O ...
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bioRxiv - Cell Biology 2021Quote: ... from pSJ1256 using primers containing the PacI and AscI (New England BioLabs) restriction sites ...
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bioRxiv - Cancer Biology 2022Quote: ... Amplicons containing the sgRNA sequences were amplified using NEBNext High-Fidelity (NEB) and their representation was analyzed by next-generation sequencing (HiSeq2500 ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA-containing chips were then subjected to Exonuclease I (NEB, M0293L) treatment for 1 hour at 37°C and were washed once with 0.1x SSC buffer ...
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bioRxiv - Plant Biology 2020Quote: pGEMHE-DEST containing GmSALT3 was linearized using SphI-HF (New England BioLabs); cRNA was synthesized using mMESSAGE mMACHINE T7 Kit (Ambion) ...
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bioRxiv - Molecular Biology 2021Quote: ... Phusion HF reaction buffer containing 1.5 mM MgCl2 (New England Biolabs Inc), 200 mM of each dNTP ...
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bioRxiv - Microbiology 2023Quote: ... in 20 μL of reaction mixture containing 1x NEB3 buffer (NEB, USA) and 1 U/μL RNasin Plus RNase Inhibitor (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA-containing chips were then subjected to Exonuclease I (NEB, M0293L) treatment for 3 hours at 55°C ...
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bioRxiv - Bioengineering 2024Quote: ... containing uracil and overhangs compatible with Thermolabile USER II digestion/ligation (NEB). Following outPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... Eluates containing MBP-fusions were applied to 5 mL amylose resin (NEB) columns and extensively washed with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The amplicon containing the pET-21+ backbone was pretreated with DpnI (NEB).
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bioRxiv - Microbiology 2024Quote: ... The amplicon containing the pET-21+ backbone was pretreated with DpnI (NEB). The assembled plasmid was introduced into DH5a(lpir) ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a hygromycin resistance cassette using BamHI-HF and NotI-HF (NEB) restriction enzymes ...
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bioRxiv - Neuroscience 2024Quote: ... containing 0.5 U Phusion HF Polymerase (New England Biolabs, Ipswich, MA, USA), 0.2 mM dNTPs (Solis BioDyne ...
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bioRxiv - Developmental Biology 2024Quote: The chip containing cDNA was then treated with exonuclease I (NEB, M0293L) at 37°C for 1 hour to cleave the cDNA from the chip ...
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bioRxiv - Genomics 2024Quote: ... 0.5% Triton X-100) containing 8 U/mL Proteinase K (NEB #P8107S) for 12-16 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: A 1 % agarose gel containing 1X Sybr Safe staining (New England Biolabs) was cast ...
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bioRxiv - Cell Biology 2024Quote: PCR mixes containing 1 U of Q5 polymerase (M0491S, New England Biolabs), 1x reaction buffer ...
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bioRxiv - Genomics 2023Quote: ... The purified products were subjected to dA tailing with 2 μL of 10X NEBuffer 2 (New England BioLabs), 0.1 μL of 100 mM dATP ...
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bioRxiv - Genomics 2024Quote: ... 2 μL of 10% Tween-20 and 5 μL NEBNext High-fidelity 2× PCR Master Mix (NEB, M0541) was added to each well sequentially ...
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bioRxiv - Biochemistry 2024Quote: ... the reaction described above included another 2 µL of mRNA cap 2′-O-methyltransferase (50 U/µL, NEB). The capping reaction was incubated at 37°C for 4 h ...
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bioRxiv - Microbiology 2020Quote: ... Cloning and purification of full length IE86 protein: Full length IE86 protein was codon optimized and cloned in pMALcx5 vector (New England Biolabs Inc.). Cells were transformed in BL-21 cells and IE86-FL-pMALcx5 transformed cells were grown in 1L luria broth (Thermofisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Phosphorylation of purified LPR1 protein was tested according to (Maldonado-Bonilla et al., 2014) using λ-protein phosphatase (New England Biolabs). In brief ...
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bioRxiv - Synthetic Biology 2023Quote: The cell-free protein synthesis (CFPS) reactions were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc) in 384-well plates (Thermofisher NUNC ...
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bioRxiv - Cell Biology 2023Quote: ... a PCR fragment containing the complete Nv-osk coding sequence was synthesized for protein expression with PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) using manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... To remove any remaining phosphorylation the GFP-Trap bound proteins were treated with 40 units of Lambda Protein Phosphatase (New England Biolabs, P0753S) at 30°C for 30min ...
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bioRxiv - Genetics 2022Quote: ... along with 6uL of EZ-Run Pre-Stained Rec Protein Ladder (Fisher BP3603-500) and 3uL of Blue Prestained Protein Standard (NEB P7718S). Gels were electrophoresed in XT Tricine Running Buffer (Bio-Rad 1610790 ...
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bioRxiv - Microbiology 2024Quote: RdnE proteins were produced using the New England Biolabs PURExpress In Vitro Protein Synthesis Kit (New England BioLabs Inc., Ipswich MA). Template DNA contained the rdnE gene and required elements specified by the PURExpress kit ...
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bioRxiv - Plant Biology 2024Quote: Purification of recombinant proteins was performed according to the protocol of the pMAL Protein Fusion and Purification System (New England Biolabs, Japan). The expression clone Maltose Binding Protein (MBP)-RLF was obtained by an LR reaction of the pMal-c5X Vector ...
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bioRxiv - Biochemistry 2024Quote: ... the DNA digestion or cleavage activity of the proteins was performed by incubating 50 nM protein with 10 ng ml -1 pUC19 plasmid (NEB N3041S), in a 20 µl reaction at 37 °C for varying time conditions in 20 mM Hepes pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein blots were incubated with primary antibodies overnight at 4°C using the above-mentioned antibodies and the Gaussia Rabbit Polyclonal Antibody (E8023S, New England BioLabs), the Influenza A NS1 Mouse Monoclonal Antibody (sc-130568 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µl 10 mM ATP (NEB, 0756), 1 µl 100 mM DTT (Cell Signaling Technology Europe ...
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bioRxiv - Cell Biology 2020Quote: ... in 1x glycobuffer 2 (New England Biolabs), and 10% NP-40 (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... and 2 μL T4 PNK (NEB, M0201L) were added to the sample in respective order ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 µl of 20 µM SpCas9 (NEB, Cat ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3.13 μL BSA 2 mg/mL (NEB), 12.5 μL FastDigest NotI (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3.13 μL BSA 2 mg/mL (NEB), 12.5 μL FastDigest NotI (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10ng of 2-log ladder (NEB N3200L) was loaded instead of 100ng ...
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bioRxiv - Genomics 2020Quote: ... in 1X Buffer 2 (New England Biolabs) at 37°C for 1 hr ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μl T4 ligase (New England Biolabs), 2 μl SrfI (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... 25 µL 2× Q5 master mix (NEB); 1 µL 10 µM TruSeq PCR handle primer (IDT) ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 μl fragmentase (New England Biolabs) and brought up to 20 μl in nuclease free water and incubated at 37°C for 20 minutes before stopping with 5 μl of 0.5M EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ribonucleoside vanadyl complex (NEB S1402S), 100 units/mL SUPERase In (Thermo Fisher AM2694) ...
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bioRxiv - Bioengineering 2020Quote: ... 2 U/µlL murine RNase inhibitor (NEB), 1.5 U/µl NextGen T7 RNA polymerase (Lucigen) ...
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bioRxiv - Neuroscience 2020Quote: ... and 2% BSA (B9000S, New England Biolabs) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 2 U of EcoRI (New England Biolabs) and one unit of T4 DNA Ligase in a total volume of 20 μL of 1X reaction buffer for one hour at 200 C ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 µl PNGase F (NEB P0704S), 3µl 10% NP40 ...