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Citations for New England Biolabs :
651 - 700 of 2065 citations for 7 7 Dimethyl 6 8 dioxaspiro 3.5 nonan 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and 2 μl of Phi29 DNA Polymerase (NEB). The reaction is incubated for 8 hours at 30°C.
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bioRxiv - Bioengineering 2023Quote: ... and mRNA Cap 2’-O-methyltransferase (NEB, M0366S). Then ...
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bioRxiv - Systems Biology 2023Quote: ... The mix contains 1x NEBuffer-2 (NEB, #B7002S), 10 µM of Randomer (custom DNA oligo ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... 2 mM vanadyl-ribonucleoside complex (New England Biolabs), and 10.6% dextran sulfate (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 µL 10x Nuclease P1 buffer (NEB) for 30 min at 37°C ...
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bioRxiv - Genomics 2024Quote: NEBuffer 2 (New England Biolabs, cat. no. B7002)
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bioRxiv - Biochemistry 2024Quote: ... 2 µM RNCs or empty 70S ribosomes (NEB) were incubated (30 min ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl T4-PNK (10 U/µl, NEB) and 9.5 µl H2OMQ were added ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μL of T4 DNA ligase (NEB M0202S) was added to the mixture and incubated overnight at room temperature to seal nicks on DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μl of 10X RNA ligase buffer (NEB), 2 μl 10 mM ATP ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of 10X CutSmart® buffer (1X final) and 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) (0.1U/μl final ...
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bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed four times with 2×SSC and stored at 4 ⁰C in 2×SSC supplemented with 1:1000 murine RNase inhibitor (NEB M0314L) prior to imaging.
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bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
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bioRxiv - Genetics 2023Quote: ... 5 µl of 100 ng/µLcDNA diluted from the previous step was combined with 2 µL of block mix and 2 µL of nuclease free water (NEB, AM9937), and then the cDNA block oligo mix was incubated on a thermocycler under the following conditions to allow block oligo mix to bind to the 5′ end and the 3′ end of the cDNA molecule ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... The final library products were further purified using an 8% polyacrylamide gel to excise 130-160nt products relative to the pBR322 DNA-MspI Digest ladder (New England Biolabs, Cat# E7323AA). The purified libraries were pooled and sequenced (single end 50 bp ...
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bioRxiv - Microbiology 2019Quote: ... 10 (GSF1697) or 12 (GSF2248) PCR cycles were run using 8-nt barcoded oligos from NEBNext Multiplex Oligos barcode kit (NEB cat# 6609S). The libraries were purified with Agencourt AMPure XP beads ...
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bioRxiv - Genomics 2022Quote: ... samples were gently removed from the chamber and digested overnight at 37°C in 8 U mL-1 proteinase K (NEB, cat# P8107) in digestion buffer (1× TAE buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... was immersed in a 10-fold volume of digestion solution with 8 U/mL Proteinase K (P8107S, New England Biolabs, Ipswich, USA) in digestion buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were amplified for 9 cycles for the hepatocyte libraries and 8 cycles for the liver organoid libraries using Phusion® High-Fidelity PCR Master Mix (NEB, M0531S) with the Nextflex primers ...
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bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
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bioRxiv - Genetics 2021Quote: ... EagI/KpnI-digested ORFs were ligated into the EagI/KpnI-digested pUASTattB vector backbone using a 6:1 insert:vector molar ratio and T4 DNA ligase (M0202S, NEB) in a thermocycler overnight (∼16 hr) ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of 1xMNase buffer containing 60 gel units/ml (6 Kunz units/ml) of Micrococcal nuclease (NEB M0247S) were added and incubated for exactly for 5 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... and subsequently mixed at a 6:1 ratio with the digested backbone in reactions containing T4 DNA ligase (NEB).
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bioRxiv - Developmental Biology 2020Quote: ... and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles ...
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bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was passed twice at a flow rate of 0.5 mL/min through a column with 6 mL chitin beads (NEB) equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: 5’-Phosphorylated linker (Oligonucleotide K, Supplementary Table 6) was adenylated using a 5’ DNA Adenylation Kit (New England Biolabs) at 20x scale and purified by TRIzol extraction as previously described56 ...
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bioRxiv - Biochemistry 2021Quote: To generate circular DNA with a single nick phagemid DNA (~2,6 μg) with or without non-denaturing bisulfite treatment was treated with 20 Units of Nt.BstNBI (New England Biolabs) for 1 hour at 37°C in 50 μl of 1X NEBuffer 3.1 followed by deactivation at 70°C for twenty minutes.
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bioRxiv - Microbiology 2021Quote: ... 4°C and the supernatant applied on a 6 ml gravity flow column with Chitin resin (New England Biolabs), equilibrated with 20 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... Cx36-SNAP transfectants were treated with 6 µM SNAP-Cell Oregon Green (New England Biolabs Inc., Boston, MA, USA) in 10% FBS DMEM for 30 min at 37°C to quench all Cx36-SNAP molecules ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... We incubated TXTL reactions at 29 °C for 6 h and digested at 37 °C with PacI (NEB R0547S) as instructed by the provider and added water instead of PacI for the undigested control ...
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bioRxiv - Genetics 2024Quote: ... in reactions containing 1X ThermoPol buffer (12.5mM tricine, 42.5mM KCl, 1.5mM MgCl2, 6% dextran; New England BioLabs, Ipswich, MA), 0.32 mM dNTP mix ...
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bioRxiv - Microbiology 2024Quote: ... protein aliquots were subjected to PNGase F or a broad range mannosidase (α1,2/3/6) (New England Biolabs, France) digestion according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg RNA were incubated for 30 minutes at 37°C with 2 μl RNA 5’ Pyrophosphohydrolase (NEB, M0356, 5,000 units/ml). The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2 uL of T4 RNA ligase I (NEB M0204S) totaling 20 uL and incubated for 1 hour at 30 C.
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10X RtcB reaction buffer (New England Biolabs), 2 μl 1mM GTP ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by adding 2 μl of RNase A (NEB) and incubating at 37 °C for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1x T4 RNA Ligase 2 reaction buffer (NEB, M0239S), and 0.5 units/µl T4 RNA Ligase 2 (NEB ...
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bioRxiv - Genomics 2019Quote: ... 5 μl of NEB Buffer 2 (New England Biolabs), 2 μl dNTP mix (2.5 mM) ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 ng of 2-log DNA ladder (NEB N3200) was included for reference ...