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Citations for New England Biolabs :
6701 - 6750 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: An aliquot of the DNA extract was placed into the NEBNext Ultra II Library preparation kit (New England Biolabs®) following manufacturer’s instructions with the following modifications ...
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bioRxiv - Molecular Biology 2021Quote: Samples were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Cat # E7645) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Microbiology 2020Quote: ... and were fused to fragment TRL2(Δgfp)-NH using the NEBuilder HiFi DNA Assembly Cloning kit (NEBuilder, NEB, Cat. #M0491) to create plasmids pTRL2-NH-EUO and pTRL2-NH-HrcA ...
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bioRxiv - Microbiology 2021Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... The RNA-Seq libraries were prepared from total RNA using the NEBNext Ultra II RNA Sample preparation kit (NEB, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Transcription of pgRNA constructs were carried out using a Hiscribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), after linearization of the DNA plasmid using HindIII ...
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bioRxiv - Microbiology 2021Quote: ... The Y-adaptor was ligated to the ends of the fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were gel purified and transcribed in vitro using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). The transcribed sgRNAs were purified using the RNeasyTM mini kit (Qiagen).
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Cell Biology 2020Quote: InsertTAG-pcDNA FRT/TO was created by mutagenesis of YFP-pcDNA5 FRT/TO39 to replace YFP with NheI and XhoI restriction sites using the primers ccctcgagGATATCACAAGTTTGTACAAAAAAGC (forward) and tggctagcAAACGCTAGAGTCCGGAG (reverse) and the Q5 Site-Directed Mutagenesis Kit (E0554S, New England Biolabs). BioID2-pcDNA5 FRT/TO was created by restriction digestion of BioID2 from myc-BioID2-MCS (74223 ...
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bioRxiv - Genomics 2019Quote: ... Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB). All end preparation ...
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bioRxiv - Genomics 2019Quote: ... We then cloned a 2:1 molar excess of our gel-extracted insert into 100 ng of the human STARR-seq vector (linearized with AgeI and SalI) with the NEBuilder HiFi DNA Assembly Cloning Kit (NEB), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Region B or Region C were used as templates for in vitro transcription by HiScribe T7 quick high yield RNA synthesis kit (NEB). The obtained RNA products were converted back to DNA oligo probes via reverse transcription (RT ...
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bioRxiv - Developmental Biology 2019Quote: ... The precipitated DNA fragments were ligated with adaptors and amplified by ten cycles of PCR using NEBNext Ultra II DNAlibrary Prep Kit for Illumina (NEB) to prepare libraries for DNA sequence determination using Illumina HiSeq2500 and single-end reads of 50bp ...
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bioRxiv - Neuroscience 2020Quote: ... Intron-containing dCas9-VPR was constructed by the insertion of a gBlock containing the SV40 intron into the original dCas9-VPR plasmid via Gibson assembly (Gibson Assembly kit, New England BioLabs). The SVI-FLEX construct was built via Gibson assembly of the original dCas9-VPR backbone and two gBlocks encod-ing the SV40 intron sequence ...
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bioRxiv - Molecular Biology 2021Quote: Double-stranded cDNA was prepared for Illumina sequencing with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs) and iTru primers ...
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bioRxiv - Genomics 2021Quote: ... The library was prepared using the ONT 1D kit (SQK-LSK108) and the NEBNext End repair / dA-tailing Module NEB Blunt/TA Ligase Master Mix (New England Biolabs) according to the kit’s manual ...
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bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were prepared from 300 ng of ribo-depleted RNA (NEBNext Ultra II Directional RNA Library preparation kit) and indexed with NEB Next Illumina indexing primers (NEB). An Agilent Bioanalyzer with high sensitivity DNA chip was used to confirm the quality of the libraries before they were sent for sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified fragmented mRNA was then used to generate cDNA libraries for sequencing using NEBNext Ultra Directional RNA library Kit (E7420S, NEB) following the protocol provided by the manufacturer with these modifications ...
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bioRxiv - Immunology 2020Quote: ... In vitro transcription of gRNAs from assembled oligonucleotides was conducted using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). Injected clutches of embryos were validated to contain CRISPR mediated mutagenesis by a T7 endonuclease assay ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragment libraries were prepared by shearing genomic DNA using a Covaris sonicator and the NEBNext Ultra II DNA library preparation kit (New England BioLabs). DNA was sequenced on a ∼40 Gb (i.e ...
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bioRxiv - Cell Biology 2019Quote: ... pSS363 and pSS364 were generated following essentially the procedure described in the Q5 site-directed mutagenesis kit (New England Biolabs) using the pSS75 plasmid as template ...
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bioRxiv - Genomics 2019Quote: ... we introduced the alternative alleles via site-directed mutagenesis (SDM) using the NEB Q5 Site-Directed Mutagenesis kit (New England Biolabs). We designed primers using NEBaseChanger (v.1.2.8) ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were carried out in 10 μl of the purified components from the PURExpress In Vitro Protein Synthesis Kit (E6800S/L, NEB) with the corresponding templates ...
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bioRxiv - Cell Biology 2019Quote: ... The two fragments were then ligated together (via the AatII digest site) using a T4 ligase (kit from New England Biolabs) and subsequently PCR amplified using primers P5 and P11 ...
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bioRxiv - Developmental Biology 2019Quote: ... Extracted DNA was prepared into ChIP-seq libraries using the NEBNext Ultra DNA library preparation kit for Illumina (New England Biolabs) and was sequenced on the HiSeq 2500 platform.
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bioRxiv - Biochemistry 2020Quote: ... at 95°C for 5 min and incubated with 80000 units of O-glycosidase and 100 units of neuraminidases for 4 hours at 37°C following the NEB kit protocol (E0540S; NEB). Samples were resolved on 15% Tris-tricine SDS-PAGE gel and blotted using anti-Cterm OCN goat antibody.
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bioRxiv - Molecular Biology 2019Quote: ... Enriched RNAs were then chemically fragmented and libraries were constructed using the NEBNext small RNA library preparation kit (NEB E7330) as previously described (13) ...
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bioRxiv - Molecular Biology 2019Quote: ... All the pCMV and pcDNA3.1-myc-eIF6 phospho-site mutants were generated using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) using the forward and reverse primers listed in Appendix Table S1 ...
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bioRxiv - Physiology 2019Quote: ... The human equivalent of the c655 mutation (G865V) was introduced into this plasmid using the Q5 Site-directed mutagenesis kit (New England Biolabs), with the following primer pair ...
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bioRxiv - Molecular Biology 2020Quote: ... Guide RNAs were cloned into a T7 promoter vector followed by in vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit, New England BioLabs) and spin column purification (RNeasy ...
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bioRxiv - Genetics 2019Quote: Library preparation for Illumina paired-end sequencing was performed with the NEBNext Ultra II library preparation kit (New England Biolabs) according to manufacturer’s protocol and barcoded using NEXTflex barcodes (BIOO Scientific ...
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bioRxiv - Genetics 2019Quote: ... 57-112) were made on the NDC80-3V5 parent vector using the Q5 Site-Directed Mutagenesis Kit (NEB, Ipswich, MA). All the single integration plasmids described above were digested with PmeI ...
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bioRxiv - Plant Biology 2019Quote: ... and the library was prepared using the NEBNext Ultra Directional II RNA Library Prep Kit for Illumina (New England Biolabs). RNA quality and quantity were checked by capillary electrophoresis (TapeStation ...
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bioRxiv - Genomics 2021Quote: The 5’- and 3’-adapter sequences are based on the adapters from the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (New England Biolabs) with the addition of 5 random nucleotides (5N) ...
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bioRxiv - Genomics 2021Quote: ... All fragments used to generate the backbone vector and subsequent backsplicing cassettes were cloned using the NEB Hifi assembly kit (NEB) following the manufacturers protocols.
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bioRxiv - Genomics 2021Quote: ... and NEBNext Multiplex reagents and sequencing adapters were used to prepare sequencing libraries following the Illumina NEBNext DNA library prep kit instructions (New England Biolabs). Two rounds of capture using a pool of biotinylated oligos (IDT ...
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bioRxiv - Genomics 2021Quote: ... Site-directed mutagenesis for amino acid substitution was performed using the Q5® Site-directed mutagenesis kit (New England Biolabs) according to the manufacture’s instruction ...
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bioRxiv - Genomics 2021Quote: ... or by ribodepletion with NEBNext Human/Mouse/Rat rRNA Depletion kit followed by library construction using the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Illumina 2×151 paired end reads were generated either on the HiSeq 4000 or NovaSeq 6000 sequencing platforms (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... 500ng input RNA was treated using the NEBNext Human/Mouse/Rat rRNA Depletion kit and libraries were prepared following the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Paired end 2×151 bp reads were produced using the HiSeq 4000 platform (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from whole bodies with a SDS protocol and libraries were constructed using NEBNext DNA Library Prep Kits (New England Biolabs). Whole-genome sequencing was performed on Illumina Novaseq 6000 (paired-end mode ...
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bioRxiv - Genetics 2021Quote: ... and purchased from IDT (Integrated DNA Technologies) to be used in the EnGen sgRNA Synthesis Kit (New England Biolabs, E3322S). Lyophilized oligos were resuspended in molecular biology grade water to a concentration of 100 uM ...
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bioRxiv - Genetics 2021Quote: Plasmid pNR127.9 was constructed by changing the first rfk-1 stop codon in pNR9.1 from TAG to TGG with primer set 1727/1728 and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Similarly ...
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bioRxiv - Genetics 2020Quote: ... The 5’ and 3’ sequences were amplified from WT adult gDNA extracted using the NucleoSpin Tissue Kit (Machery-Nagel) by PCR using Phusion High Fidelity PCR Master Mix (NEB) and primers 5’-ttttgcggccgcTCTGTTTGAATATGTTTCCGAGAA −3’ and 5’-ttttctcgagTTTCCGCGACAAAAACACAGA-3’ which add restriction enzyme recognition sites NotI and XhoI ...
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bioRxiv - Evolutionary Biology 2021Quote: Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA; Fig. 2). Size selection was not employed for samples with highly degraded DNA ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs). Selected genes were amplified by quantitative real time PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2021Quote: Plate-based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (catalog no. E6420L; New England Biolabs). Total cell fractions from dissociated gut sections of donors BRC2033-2034 ...
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bioRxiv - Cell Biology 2021Quote: RNA used for polyA selected library generation for mRNA sequencing was isolated with the Monarch Total RNA isolation miniprep kit (New England Biolabs). Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508 ...
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bioRxiv - Genomics 2021Quote: ... and the PCR products were inserted into the SmaI site of the pPK6 vector (78) using a Gibson assembly cloning kit (New England Biolabs). This allows the expression of cloned genes under the control of the GAL1 promoter ...