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Citations for New England Biolabs :
6001 - 6050 of 6282 citations for 6 Quinolinamine 1 ethyl 1 2 3 4 tetrahydro 2 2 4 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... purified Trx-FER-KD and FER-KDK565R proteins were treated with 1 μl of λ-phosphatase (λ-PP) (400,000 units/ml, New England Biolabs. P0753S) and 1 mM MnCl2 for 1-2 h at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the truncated versions of cwr-1 and the histidine mutant plasmids were prepared using the Q5 Site-Directed Mutagenesis Kit (E0552S NEB, Ipswich, MA). Gibson assembly (E2611S NEB ...
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bioRxiv - Microbiology 2020Quote: ... samples were washed in phosphate buffered saline (PBS) and labelled with 1:500 of 10mg/mL Hoechst 33342 (New England Biolabs, Cat# 4082S) for 10 min with a subsequent wash in PBS ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of total RNA was used as template for complementary DNA synthesis using MuLV reverse transcriptase (New England Biolabs, Cat. M0253L) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 6ul eluted RNA was used for Small RNA libraries using NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) (NEB, Cat# E7300S) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Small RNA libraries were prepared from the extracted 1ug total RNA or 100ng PIWIL1-antibody-pulled down RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) (NEB, Cat# E7300S) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibodies were diluted in the same Triton X 100 and BSA solution and suppliers and source were: primary 1:200 anti-CK20 rabbit D9Z1Z (New England Biolabs, Herts, UK), anti-CD31 mouse JC70/A (Abcam ...
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bioRxiv - Genomics 2020Quote: ... 3 μl from 100 μl of enzymatically converted and 1 μl from 15 μl of bisulfite-converted DNA were PCR amplified with Q5U polymerase (NEB, Ipswich, MA) and primers (Supplemental Table 3 ...
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bioRxiv - Neuroscience 2021Quote: ... with 1% (w/v) octylglucoside containing 5 U endoglycosidase H (Endo H) or peptide: N-glycosidase F (PNGase F; both NEB, Frankfurt, Germany) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR reactions were pooled and treated for 1 hr at 37°C with 25 U of Exonuclease I (NEB, Cat# M0293S) per 100 μL of pooled PCR reactions ...
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bioRxiv - Genetics 2022Quote: The full-length ccf-1 cDNA clone was generated through PCR using the Q5® High-Fidelity DNA polymerase (New England BioLabs, M0491L) and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD ...
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bioRxiv - Genomics 2022Quote: Poly-A libraries (figure 1 and related data) were constructed with the NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs, Ipswich, USA), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with TBS and incubated with a horseradish peroxidase (HRP)-conjugated anti-MBP monoclonal antibody (New England Biolabs; 1:5000).
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bioRxiv - Cell Biology 2022Quote: ... SNAP-GLP-1R and SNAP-GIPR were detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/1,000) followed by goat anti-rabbit HRP secondary (ab6271 ...
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bioRxiv - Microbiology 2019Quote: ... Before loading the samples and molecular weight ladder (Quick-load Purple 50 bp DNA Ladder and Quick-load Purple 1 kb DNA Ladder, New England Biolabs Inc, UK) to the gel ...
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bioRxiv - Microbiology 2019Quote: ... 20 µl of template, 20 U of exonuclease I, 5 U of Antarctic phosphatase, 1× Antarctic phosphatase buffer; New England Biolabs, Frankfurt, Germany); incubation conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... the NEBNextUltra™ II Directional RNA Library Prep Kit for Illumina and the NEBNext® MultiplexOligos for Illumina® (Dual Index Primers Set 1) according to the manufacturer’s protocols (New England Biolabs, USA). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2021Quote: ... PCR products with homologous DNA sequences (1 to 1.5 kb) flanking the deleted region were PCR-amplified with Phusion High-Fidelity DNA Polymerase (NEB, Ipswich MA, USA). These flanking PCR products were fused to two sides of either a NEO or NAT dominant selectable marker via overlap PCR ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes were incubated overnight at 4 °C with the appropriate antibody: rabbit monoclonal anti-GSK3β (1:1,000; product # 9315, Cell Signalling Technology, New England BioLabs, Whitby, Ontario, Canada), rabbit polyclonal anti-p[Ser9]GSK3β (1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... and BchB were PCR amplified from Rhodobacter sphaeroides genomic DNA and cloned into pRSF-Duet 1 or pET-Duet 1 plasmids as described.12 Mutations in BchL were generated using Q5 site-directed mutagenesis (New England Biolabs, Ipswich, MA). Plasmids used to express the linked-BchL-proteins carrying glycine linkers of various lengths were synthesized as codon-optimized genes (Genscript Inc. ...
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bioRxiv - Genomics 2021Quote: ... the adapter ligation was performed using 2.5 µl of NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1 - NEB, cat# E7416S); the post-adapter ligation purification was performed using 50 µl of purification beads and 50 µl of purification beads’ buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The heat-denatured samples were incubated at 37 °C for 1 h with 500 units of Endo H (endoglycosidase H; New England Biolabs, Ipswich, MA) in a final volume of 20 µl containing 1 × GlycoBuffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using 5 units of MseI enzyme per 1 μg of DNA and 5 μl of 1X SmartCut™ Buffer (New England Biolabs®). This was followed by the incubation for 45 min at 37°C and inactivation for 20 min at 65° C ...
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bioRxiv - Biochemistry 2020Quote: ... Excess iodoacetamide was quenched with DTT and the samples were digested overnight with endoproteinase GluC (1:100) at room temperature (New England Biolabs, Product #P8100S). Digested peptides were dried in a vacuum concentrator ...
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bioRxiv - Molecular Biology 2021Quote: ... XIST genomic regions were PCR amplified from the genomic DNA extracted from NT2/D1cells using specific primer pairs (Supp Table 1) followed by restriction enzyme digestion of the vector and insert and ligation using T4 DNA ligase (New England Biolabs, MA, USA). The positive clones were confirmed by sequencing.
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bioRxiv - Genetics 2020Quote: ... Zebrafish embryos were injected at the 1-cell stage with a 1 nL solution of bbs2 gRNA (200 ng/μL) and Cas9 protein (10 μM; New England BioLabs, Beverly, MA). Mutagenesis was confirmed by High Resolution Melt Analysis (HRMA ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR product were circularized by intra-molecular self-ligation in 1 ml of ligation buffer using T4 DNA ligase (New England Biolabs, Ipswich, MA). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... Additional reconstitutions with linker histone were performed by mixing reconstituted 183×12 core arrays with 1 mg/ml recombinant linker histone variant H10 (New England Biolabs, cat.# M2501S) at a molar ratio of 0.7 molecule histone H1 per nucleosome in solution containing 500 mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... PCR products (∼1.1 kb-long flanking unique sequences on either side of each rDNA locus) were labelled using Klenow Fragment exo- (NEB CAT. M0212L) with either Biotin-16-dUTP (Roche CAT ...
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bioRxiv - Cell Biology 2022Quote: ... INS-1 832/3 SNAP- GLP- 1R or SNAP-GIPR cells were labelled in suspension with 40 nM SNAP- Lumi4- Tb and 1 mM SNAP- Surface 649 (New England Biolabs, Hitchin, UK) for 1 hr at room temperature in complete medium ...
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bioRxiv - Genomics 2022Quote: ... samples were gently removed from the chamber and digested overnight at 37°C in 8 U mL-1 proteinase K (NEB, cat# P8107) in digestion buffer (1× TAE buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Molecular Biology 2023Quote: ... The digested T/F LTR U3R region was cloned into the linearized pGL3 Basic vector/plasmid and ligated using 1 U of T4 DNA ligase (New England Biolabs, MA, USA) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: RE digestion reactions are prepared by adding 1/10th of the final reaction volume of 10x reaction buffer supplied by the RE manufacturer (typically NEB CutSmart buffer) and 0.1% Tween-20 to the DNA solution (diluted to the desired concentration in nanopure DI water) ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was purified from 1 μg of total RNA using an NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... the upstream and downstream probes of DLP pairs was ligated by applying a ligation mix containing 1× SplintR buffer (Cat No. B0375S; NEB, Beijing, China), 2.5 U/μL SplintR Ligase (Cat No ...
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bioRxiv - Cell Biology 2022Quote: ... PRR14 1-212 PCR products were sub-cloned into the expression vector pCMV6-AN-mGFP using T7 ligase (New England Biolabs, cat# M0318S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The kinesin and GBP constructs were exchanged into 1x PBS with 1 mM DTT and labeled with DNA and SNAP-Surface Alexa Fluor 647 (NEB, Ipswich, MA) dye directly after elution ...
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bioRxiv - Systems Biology 2024Quote: ... Eluted glycopeptides were lyophilized and then deglycosylated by incubation with 50 µL of 1 unit per microliter of PGNase F (New England Biolabs, Ipswich, MA) in 50 mM Tris-HCl (pH=7.5 ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared with 1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalog #E6560L) as instructed by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... GST-IpaH4-3XFlag was cloned into pGEX6P-1 with a 3XFlag peptide followed by the coding sequence for ubiquitin using Gibson Cloning (NEB; Table S4). Sequence-verified constructs were transformed into E ...
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bioRxiv - Immunology 2024Quote: ... The region after the TRR to the middle of exon 1 was amplified from 50 ng of gDNA using Phusion® High-Fidelity polymerase (New England Biolabs, M0530) according to the manufacturer’s instructions with the following primers ...
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bioRxiv - Microbiology 2024Quote: ... was reverse transcribed from ∼1 μg high quality RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEW ENGLAND BioLabs, Germany). Newly synthesized cDNA was purified using a the NucleoSpin® Gel and PCR Clean-up kit (Germany ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genomics 2024Quote: ... RNA from each sample was enriched from 1 µg total RNA by the NEBNext® Poly(A) mRNA Magnetic Isolation Module (Catalog #: E7490, New England Biolabs Inc.). RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog # ...
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bioRxiv - Neuroscience 2023Quote: ... appropriate number of amplification cycles x (98C - 10sec, 63C – 30sec, 72C – 1 min) with NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) and barcoded Nextera primers (1.25 μM each ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, catalog #E6560S) with oligo-dT priming according to the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were propagated in Escherichia coli NEB Turbo ((F’ proA+B+ lacIq ΔlacZM15/fhuA2 Δ(lac-proAB) glnV galK16 galE15 R(zgb-210::Tn10) TetS endA1 thi-1 Δ(hsdS-mcrB)5)) (New England Biolabs) at 37°C in lysogeny broth (LB ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...