Labshake search
Citations for New England Biolabs :
5801 - 5850 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... DNA was extracted similarly to WGBS protocol above and processed using the NEBNext Enzymatic Methyl-seq Kit (NEB E7120S) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: RNA-Sequencing libraries were generated by using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Immunology 2023Quote: ... and assembled into SalI linearized LentiGuide-GBP-Chr3 sgRNA3 plasmid using HiFi DNA Assembly Kit (New England Biolabs, E5520). Primers used were listed in Supplementary Table S3.
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bioRxiv - Microbiology 2023Quote: ... Mutant constructs were built with the complementation vector as a template using the Q5 Site-Directed Mutagenesis Kit (NEB) to mutate specific amino acids (primers P97-P100 ...
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bioRxiv - Cell Biology 2023Quote: ... and libraries for massive parallel sequencing were constructed with NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB). Library quality was assessed with Agilent Bioanalyzer 2100 system ...
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bioRxiv - Neuroscience 2023Quote: NGS Libraries were made from eluted DNA using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... was generated from full-length CSN-5 using a Q5 site-directed mutagenesis kit per manufacturer’s instructions (New England BioLabs). Truncated CSN-5 constructs were made by PCR from full-length CSN-5 and inserted into pGEX-KG plasmids ...
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bioRxiv - Developmental Biology 2023Quote: ... The library was prepared as per the user’s manual using the NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S, NEB). Library indexing was done with NEBNex multiplex oligos for Illumina Set 1 (E7335S ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs Inc.) and index codes were added to attribute sequences to each sample ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR amplified product was in-vitro transcribed using the HiScribe T7 High Yield RNA synthesis kit (NEB #E2040S) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... a DNA adapter (TableS6) was ligated to 3′ ends of nascent RNA using the T4 RNA ligase kit (NEB) by mixing 50 pmol adapter with 300-600 ng nascent RNA ...
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bioRxiv - Genetics 2022Quote: ... Variants were generated in the pDONR vector via site-directed mutagenesis utilizing NEBaseChanger with the Q5 mutagenesis kit (NEB) and verified by sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA samples (500ng) were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit (NEB #E6420) by following manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs #E7645L) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina according to the manufacturer’s protocol (NEB) and paired-end sequencing was performed with NovaSeq 6000 platform (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed to amplify libraries using the NEBUltra Ultra II DNA library prep kit for Illumina (NEB, E7645L). The final libraries were purified using AMP Pure XP beads (Beckmann Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina® (NEB England BioLabs). RNA-seq paired-end reads were sequenced on the Illumina NovaSeq 6000 platform.
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bioRxiv - Cell Biology 2022Quote: ... RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina® (NEB England BioLabs). RNA-seq paired-end reads were sequenced on the Illumina NovaSeq 6000 platform.
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bioRxiv - Cell Biology 2023Quote: ... T690A mutation of RAD50 and S1336A mutation of BRCA1 were introduced using the Q5 Site-directed mutagenesis kit (NEB). The MAP7 truncation mutations were created by PCR cloning to Gateway p221 pDONR221 (Invitrogen) ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645S) with the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... were further assembled and sequenced at GIGA-Liège University (Belgium) using the NEBNext Ultra II DNA library prep kit (New England BioLabs) and then sequenced on the Illumina NextSeq 500 platform with 2 × 150 nt reads with a target of 10 million sequences per pool of ten libraries (i.e ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA was submitted to library preparation using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E765). The samples were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genetics 2023Quote: ... The sgRNA was synthesized using the NEB HiScribe T7 Fast and Efficient RNA Synthesis Kit (NEB E2050S, MA, USA). After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646 ...
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bioRxiv - Genetics 2022Quote: ... exon duplication and exon deletion mutants were generated by site-directed mutagenesis PCR following the manufacturer’s instructions of the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). To generate the plasmids for in vitro transcription/translation of the fusion proteins SmMADS3-GFP and CRM3-GFP ...
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bioRxiv - Immunology 2023Quote: pals-17::gfp and pals-20::wrmScarlet translational reporter constructs were assembled using NEBuilder HiFi kit (New England Biolabs) by fusing PCR products and gene fragments ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: DNA for WGS was prepared by PCR-free library preparation according to the NEBNext DNA Ultra II kit (NEB). Sequencing was performed by Illumina NextSeq 2000 P3 150PE.
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bioRxiv - Immunology 2023Quote: 1 μg of total RNA was subjected to rRNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... of ATG13 and ATG13.11-198 referred to as ATG13.1HORMA were introduced into linearised pBMN-HA-C1 using Gibson Cloning kit (New England Biolabs) to create pBMN-HA-ATG13 and ATG13.1HORMA (Addgene #186223 and #18622 respectively) ...
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bioRxiv - Molecular Biology 2023Quote: DNA templates were transcribed and translated in vitro using a custom PURExpress ΔRF-123 ΔRibosome Kit (New England Biolabs) and a final concentration of 2.2 μM ribosomes ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using NEBNext Ultra II RNA library prep kit using the manufacturer’s instructions (NEB, Ipswich, MA). Sequencing libraries were validated on the Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as via quantitative PCR (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 pmol of TP_MAAAPQKCAAA* mRNA (see “Toeprinting assays” section) and components of the PURExpress ΔRibosomes Kit (New England Biolabs). The reaction was incubated at 37ºC for 20 min and then diluted in 50 mM Hepes KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Plant Biology 2023Quote: ... Poly-A enriched libraries were prepared using the NEBNext UltraTM RNA Library Prep Kit (New England Biolabs, Ipswich, USA) for Illumina ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Systems Biology 2023Quote: ... metatranscriptomic libraries were constructed according to the NEBNext® UltraTM RNA Library Prep Kit (Illumina, New England Biolabs, MA) and sequenced on the Illumina Hiseq 2500 platform (Illumina ...
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bioRxiv - Systems Biology 2023Quote: ... Dual-indexed sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) with 10 ng or 10 uL of input DNA (whichever was lower volume) ...
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bioRxiv - Genomics 2023Quote: ... Preparing the MLI1 DNA library was performed using NEBNext Ultra II DNA Library Prep kit (New England Biolabs, USA) and sequencing on an Illumina MiSeq with paired-end 300 bp reads at the “Molecular and Cellular Biology” core facility of the IMCB SB RAS (Novosibirsk ...
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bioRxiv - Cell Biology 2023Quote: ... APOE3-C-mEm ΔSS were created from APOE3-mEm using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). The N-terminal domain constructs consist of residues 19-209 with or without residues 1-18 of the N-terminal signal peptide ...
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bioRxiv - Developmental Biology 2023Quote: ... Eluted DNA fragments were amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ChIP sequencing libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit reagents (NEB, Ipswitch, MA) according to the procedure recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... which was subsequently cloned into linearized pUHE-21 (106) using NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Construct was verified by Sanger DNA sequencing with primers 146 and 156.
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bioRxiv - Microbiology 2023Quote: The pGEX-6P-PrkA1-338 plasmid (Table 2) was constructed by a two-part ligation (NEB Quick Ligation kit) of BamHI- and NotI-linearized pGEX-6P and PCR-generated prkA1-338 amplified with primers AS21 and AS81 (Table 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Immunology 2023Quote: ... RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequencing library was generated using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs, USA). The concentration and quality of the library was analysed with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... low range ssRNA ladder (N0364S) and the Monarch RNA Cleanup Kit (T2030L) were purchased from NEB (Ipswich, MA, USA). T4 DNA ligase (EL0013) ...
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bioRxiv - Plant Biology 2023Quote: ... the cDNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). The resulting libraries were measured using the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... mRNA was reverse transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (BioLabs, Ipswich, MA, USA). Ribosomal Protein L32 (RPL32 ...
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bioRxiv - Cell Biology 2023Quote: ... Envelope gene mutations were introduced into WT cDNA sequence using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). All cloned were sequenced (Eurofins).
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bioRxiv - Genomics 2023Quote: ... libraries were constructed with the NEBNext Small RNA Library Preparation Kit according to manufacturer’s recommendations (NEB, cat. no. E7330). See Supplemental File 6 for detailed protocol.