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Citations for New England Biolabs :
5651 - 5700 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs) according to manufacturer’s instructions in combination with a poly(A ...
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bioRxiv - Cancer Biology 2024Quote: ... Mutations and insertions were introduced by site directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs). Oligonucleotides for site-directed mutagenesis were designed using the NEBaseChanger version 1.3.3 (New England Biolabs ...
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bioRxiv - Physiology 2022Quote: ... cDNA was prepared following the NEBNext® Ultra™ II Directional RNA Library Prep Kit protocol (New England Biolabs). The libraries were sequenced using paired-end 150 bp sequencing on a HiSeq 4000 System (Illumina) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with a primer/probeset binding in the orf1ab region (forward ...
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bioRxiv - Molecular Biology 2022Quote: ... and in vitro transcription was performed using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA, USA) to generate IVT mRNA according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP-Seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on a NovaSeq 6000 ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-seq libraries were prepared with NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolabs #E7420S) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... in high output runs using a NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) including rRNA depletion ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant plasmids encoding a catalytically inactive 3Dpol (3Dneg) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). Nucleotides at position 6891 and 6892 of the CVB3/28 genome were mutated from AT to GC ...
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bioRxiv - Microbiology 2022Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using the manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by T7 in vitro transcription using the HiScribe T7 In Vitro Transcription Kit (New England Biolabs, Ipswich, MA) and purification with Trizol (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and ChIP libraries were amplified and barcoded using the NEBNext Ultra II DNA library preparation kit (New England Biolabs) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged ORF3c mutant Q5Y was prepared by site-directed mutagenesis using a commercial kit (New England Biolabs, E0554S).
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared as in [34] with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).
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bioRxiv - Molecular Biology 2024Quote: ... followed by the GFP sequence were generated by using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, E2060). 1 µg mRNA was first denatured at 65°C for 3 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... In vitro transcription was performed using the HiScribe T7 ARCA mRNA (with tailing) kit (New England Biolab, http://www.NEB.com) and Xenopus oocytes were injected with 30 ng of RiSKC3 cRNA using a pneumatic injector ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet was air dried and then resuspended in DNA Elution Buffer (Monarch DNA Gel Extraction Kit, NEB, USA). Since the RPS27AP5 sequence ...
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bioRxiv - Bioengineering 2024Quote: ... and A-tailed using the NEB Next Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Linkers were then ligated to the DNA using the NEB Next Ultra II Ligation Master Mix and Ligation Enhancer (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... C-terminal Cx36 mutants were generated using the Q5 site directed mutagenesis kit (E0554S; New England Biolabs, Ipswitch, MA) and corresponding primer pairs introducing gaps into the Cx36 coding sequence ...
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bioRxiv - Biochemistry 2024Quote: ... All point mutations in PLD3 and PLD4 were generated using Q5-site directed mutagenesis kit (New England Biolabs #E0554S).
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bioRxiv - Bioengineering 2024Quote: ... Nluc mRNA was synthesized using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs Inc., MA, USA) with CleanCap Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Genomics 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep kit with poly(A) mRNA isolation was used to construct RNA-seq libraries according to the manufacturer’s instructions (NEB). Sequencing reads were aligned to hg19 build of the human genome using HISAT2 (Kim et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#). Libraries were pooled and sequenced on an Illumina NextSeq 550 using paired-end sequencing of 42 bases per read.
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bioRxiv - Cell Biology 2024Quote: MHC-ftz-Δi mRNA was synthesized in vitro using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) with MHC-ftz-Δi in pcDNA3.0 (30 ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared by on-bead reactions using the NEB Next Ultra II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand ...
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were constructed using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) using ChIP products from “OSC ChIP” and “Ovary ChIP” ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were then constructed using the NEBNext Ultra II RNA Library prep for Illumina kit (NEB#E7760L) NEBNext Poly(A ...
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bioRxiv - Microbiology 2024Quote: ... an equimolar pool of all amplicons was prepared and purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). After Qubit quantification using Qubit® dsDNA HS Assay Kit and Qubit 2.0 fluorometer (Thermo Fisher ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... Library preparation was carried using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103). Sequencing was performed on an Illumina HiSeq4A to produce paired-end reds of 125 bp ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, #E7103) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Deglycosylation of total brain homogenates was performed according to the PNGase F kit instruction manual (#P0704S, New England Biolabs). 40µg of protein were digested with 1,000 U of PNGase F enzyme.
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bioRxiv - Immunology 2024Quote: ... mRNA was purified from 4 µg of total RNA with a magnetic mRNA isolation kit (New England Biolabs, S1550S). cDNA was synthesized from mRNA samples with M-MLV (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... The libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA), and the preparation involved a polyA selection method ...
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bioRxiv - Neuroscience 2024Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) and subsequently sequenced by Illumina NovaSeq 6000 or Illumina NextSeq 500/550 (LabGenomics ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs), validated on TapeStation (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA (300 ng) was depleted of ribosomal RNA using the NEBNext rRNA Depletion Kit (NEB Cat#E6310). rRNA-depleted RNA was purified using RNAClean XP beads (Beckman-Coulter Cat#66514) ...
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bioRxiv - Neuroscience 2024Quote: ... Mutations of PICK1 (KD-AA and 5K-E) were made with Q5® Site-Directed Mutagenesis Kit (NEB #E0554S). The constructs were purified by NucleoBond® Xtra Midi EF (MACHEREY-NAGEL) ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs, USA). 4 μL of cDNA was added to 1 μL of pre-purchased primers (QuantiTect primers (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... After DNA isolation libraries were prepared with the NEBNext ULTRA II DNA Library Prep Kit for Illumina (NEB, US) according to manufacturer’s specification with the NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: We prepared the DNA extracts into sequencing libraries following the NEBNext Ultra II FS DNA Library Prep Kit (NEB) according to manufacturers’ instructions but replaced the NEBNext Adapters with Y-Adapters ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and gel excision of DNA between 150 bp and 1,500 bp was performed using the Monarch DNA Gel Excision Kit (NEB). Concentrations of the purified libraries were measured using PicoGreen (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: Small RNA libraries from immunoprecipitated RNAs or total RNA were prepared using Small RNA Library Prep Kit (E7300, NEB) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified DNA was inserted into the Hsp68-lacZ vector using the Gibson Assembly kit (E2611S, New England Biolabs). The resulting constructs were verified ...
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bioRxiv - Developmental Biology 2024Quote: ... and both reverse transcription and PCR were performed using the Luna One-Step Universal RT-qPCR kit (NEB, E3005S) and carried out on the Mic qPCR Machine (BioMolecular Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... and mRNA was transcribed in vitro from the templates using HiScribe T7 ARCA mRNA Kit with Tailing (NEB, E2060) or mMESSAGE mMACHINE SP6 Transcription Kit (Thermo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, #E7760S) and sequenced on an Illumina NextSeq2000 platform to generate a minimum of 25-30 million 150-bp paired-end reads per sample.
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bioRxiv - Genomics 2024Quote: ... Poly-A selected libraries were prepared with a NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced using the NovaSeq 6000 (Ilumina ...
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bioRxiv - Genomics 2024Quote: Methylation enrichment of cfDNA was performed using the EpiMark Methylated DNA Enrichment Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and p.V126V were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, MA; catalog no. E0552). Primers used for site-directed mutagenesis are given in Appendix 1-table 7 ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...