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Citations for New England Biolabs :
5701 - 5750 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... was used to generate libraries with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Cat# 7645). The resulting libraries were sequenced (single end 50 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... The library preparation was done using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760S). The libraries were multiplexed (six samples per lane ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA-seq libraries were prepared using Total RNA-seq ScriptSeq Library Prep Kit for Illumina (New England Biolabs) and sequenced using the HiSeq 50 cycle single-end reads protocol on the HiSeq 2500 system ...
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bioRxiv - Evolutionary Biology 2020Quote: ... library preparation was carried out using NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (New England Biolabs) with modified protocol23 for improving low-input samples and sequenced with NextSeq500 (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... And sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). mRNA was selected by purifying polyA+ transcripts (NEBNext Poly(A ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and sequencing libraries were prepared using the NEBNext® Ultra™ II DNA Kit (New England Biolabs, Massachusetts, USA) with a modified protocol to account for fragmented DNA ...
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bioRxiv - Microbiology 2021Quote: ... and adapter ligation reactions were performed on the fragmented DNA using the NEBNext Ultra II kit (New England Biolabs). Illumina paired-end sequencing was performed on a NextSeq500 device at the Georgia Genomics Facility (University of Georgia).
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bioRxiv - Evolutionary Biology 2021Quote: ... Libraries were constructed with the NEBNext® Ultra™II DNA Library Preparation kit (New England Biolabs Inc, USA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Sequencing libraries were generated using NEBNext®Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Polymerase chain reaction (PCR) products and plasmids were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Genomics 2020Quote: ... Then paired-end libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and 3Gb paired-end reads of 150bp were produced for each library.
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bioRxiv - Microbiology 2022Quote: ... we first generated two plasmids encoding guide RNAs targeting either MMCAP2_0582 or MMCAP2_0575 by modification of the base plasmid p426-SNR52p-gRNA.Y-SUP4t (25) using the Q5 Site-Directed Mutagenesis kit (NEB). In addition ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs #E7805) and Multiplex Oligos for Illumina (NEB #E7500) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for mRNA purification followed by a NEBNext® Ultra(tm) II Directional RNA Library Prep Kit (New England BioLabs) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, Massachusetts, U.S.) and sequencing was carried on an Illumina® NovaSeq™ 6000 platform ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was reverse-transcribed according to the manufacturer’s instructions using the ProtoScript Strand cDNA Synthesis kit (New England Biolabs) as previously described (Nantie et al. ...
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bioRxiv - Neuroscience 2021Quote: RNAs were in vitro transcribed from PspOMI-linearized pcDNA3.1(+) reporter plasmids using HiScribe T7 High Yield RNA Synthesis Kit (NEB) with 3’-O-Me-m7GpppG anti-reverse cap analog (ARCA ...
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bioRxiv - Molecular Biology 2021Quote: Libraries for next-generation sequencing were prepared using the NEBNext Ultra II DNA library prep kit (New England Biolabs) following the manufacturer’s instructions and sequenced on an Illumina NextSeq 500 platform (75 bp reads ...
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bioRxiv - Molecular Biology 2020Quote: EM-seq library construction was performed using the NEBNext Enzymatic Methyl-seq Kit (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions with modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Second end processing and library amplification were performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g ...
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bioRxiv - Molecular Biology 2021Quote: sgRNA products synthesized by T7 RNAP WT and S43Y mutant were purified with RNA purification kits (New England Biolabs) before analyzed by high performance liquid chromatography (HPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... SSS reaction products (16 μl) were combined with 24 μl T7 High Yield RNA Synthesis Kit (NEB, Cat #E2040) reagent mix containing 4 μl T7 Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... fragmented and processed into an Illumina sequencing library using the NEB Ultra II FS kit (NEB cat. no. E7805S). Libraries were paired-end sequenced on an Illumina HiSeq 4000 instrument.
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product was purified using Monarch PCR and DNA Cleanup kit (New England Biolabs, Inc., Ipswich, MA, USA) and DNA concentration was quantified using NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The S358A mutation was introduced into the STING ORF in this construct using Q5 site-directed mutagenesis kit (NEB). Virus generation from these constructs and cell transduction were as described (Sidorova et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 1600 v/10 ms /3 pulses for 200,000 cells in Buffer R (Neon Transfection kit) premixed with 50 pmol Cas9 protein (CAT#M0646T, New England Biolabs), 50 pmol single guide RNA (sgRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... m6A pulldown was performed with a rabbit monoclonal anti-m6A antibody in the EpiMark N6-Methyladenosine Enrichment Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Recombinant C-terminally FLAG-Tagged TEX264 was produced by PURExpress in vitro protein synthesis kit (E6800; New England Biolabs). Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B ...
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bioRxiv - Cell Biology 2022Quote: IP samples were prepared for high-throughput sequencing by using the Ultra II DNA Library Prep kit (NEB E7645L). Samples were multiplexed and paired-end sequencing was performed using a MiniSeq (Illumina ...
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bioRxiv - Genomics 2022Quote: ... sequencing libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, MA). Library preparation and sequencing were performed at the University of Missouri Genomics Technology Core ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Intact total RNA was reverse transcribed into cDNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) according to manufacturer’s instructions with random primers ...
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bioRxiv - Genomics 2020Quote: ... libraries were quantified by qPCR using either the NEBNext® Library Quant kit (New England Biolabs, Cat. N: E7630S) or the KAPA Library Quantification Kit (scRNA-seq libraries only ...
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bioRxiv - Genomics 2019Quote: ... Strand-specific sequencing libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries from two biological replicates were sequenced on the Illumina NextSeq 500 platform resulting in 22-25×106 single-end reads ...
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bioRxiv - Genomics 2020Quote: ... RT adapters (supplied in the kit) were ligated to the RNA strand using T4 DNA ligase (New England Biolabs). Purification steps were performed using Agencourt AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: Micro-C sequencing library was generated by using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) with some modifications ...
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bioRxiv - Genomics 2020Quote: ... Sequencing library preparation was done using the NEB Next® Ultra™ RNA Library Prep Kit (NEB, Ipswich, USA). Libraries (350 bp insert size ...
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bioRxiv - Immunology 2021Quote: ... Dephosphorylation was performed on the respective µRNA with addition of 5µg to a Quick dephosphorylation kit (New England BioLabs). Dephosphorylated µRNA was again purified using µRNA isolation columns ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were produced with NEBNext Multiplex SmallRNA Library Prep Kit for Illumina (E7560S, E7580, New England Biolabs) with the customisation of a dilution of primers 1:2 ...
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bioRxiv - Biochemistry 2019Quote: ... The point mutation of loa22 variants were obtained by using Q5® Site-Directed Mutagenesis Kit (NEB, Ipswich, MA) with their relevant primers ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted from the cocktail culture by Monarch Total RNA Miniprep Kit including DNase I (NEB, Ipswich, MA) which was confirmed by multiplex RT-PCR and gel electrophoresis ...
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bioRxiv - Microbiology 2019Quote: Additional mutant variants were generated with the oligonucleotides listed in Table S7 and using a protocol that adhered to the manufacturer’s instructions of the Q5 SDM kit (NEB). The protocol contained several modifications ...
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bioRxiv - Microbiology 2020Quote: ... were carried out using specific primers (Supplemental Table 1) and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). After sequence verification ...
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bioRxiv - Genomics 2020Quote: ... 500ng of total RNA was subjected to ribosomal-RNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Qualified DNA were interrupted into fragments and constructed to library using Next Ultra DNA Library Prep Kit (NEB, USA), then sequenced on the Illumina plantform ...
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bioRxiv - Molecular Biology 2019Quote: ... we followed the manufacturer’s protocol for the NEBNext® Ultra™ II Directional RNA Library Prep kit (NEB, E7765S) and poly(A ...
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bioRxiv - Genomics 2019Quote: ... 16 libraries for each histone mark) using NEBNext Ultra II DNA Library Prep kit for Illumina (NEB, cat. # E7645). NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Developmental Biology 2019Quote: Pceh-23_L::acy-2 fragment (sense/anti-sense) Pceh-23_L::acy-2 fragment(sense) was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
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bioRxiv - Developmental Biology 2019Quote: ... libraries were generated using the NEBNext Ultra II library preparation kit with 15 cycles of PCR amplification (NEB E7645). The libraries for the ‘MicroChIP’ samples were generated using the MicroPlex library prep kit (Diagenode C05010012 ...
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bioRxiv - Cell Biology 2019Quote: ... were modified based on the pcDNA3.1(+)-C-I30-FLG-TEV plasmid via the Q5 Site-Directed Mutagenesis Kit (cat#: E0554S, NEB). pCMV-FLAG-ABCE1 was a gift from Dr ...
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bioRxiv - Developmental Biology 2019Quote: ... This was used as input for the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (NEB #E7300) according to the manufacturer’s recommendations with the following modifications ...