Labshake search
Citations for New England Biolabs :
5601 - 5650 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis and RT-qPCR was carried out using Luna® Universal One-Step RT-qPCR Kit (NEB) according to the manufacturer’s instructions with 100 ng of mRNA ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified flexizyme was desalted using a Monarch RNA purification kit using 2X volume ethanol (New England Biolabs), concentration determined using a Nanodrop ND-1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... three 25 μl PCR reactions were pooled and purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Micro-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) following77 manufacturer instructions and sequenced on the Illumina NovaSeq 6000 platform.
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bioRxiv - Molecular Biology 2023Quote: ... and used to construct Illumina sequencing libraries with NEBNext Ultra Library DNA Prep Kit for Illumina (NEB, E7370). BrdU-labeled nascent DNA library fragments were then enriched by immunoprecipitation with anti-BrdU antibody (BD ...
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bioRxiv - Genomics 2023Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB #E7645). End repair was carried out by resuspending beads in 50 μL 1 × EB buffer ...
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bioRxiv - Genomics 2023Quote: ... CBS 112042+ and CBS 124.78+ were sequenced on R9.4.1 flowcells using the LSK108 kit with 3 μg DNA as input for the end prep reaction (NEB ULTRA-II EP ...
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bioRxiv - Genetics 2023Quote: ... Both point mutations were generated by site directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) using the wild type part-containing plasmids as the template and the following primers ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from dissected E14.5 DRG was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was synthesized with iScript cDNA synthesis kit (Biorad ...
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bioRxiv - Genomics 2023Quote: ... The product was converted to double stranded DNA using the NEBNext second strand synthesis kit (New England Biolabs). Library preparation was carried out using a KAPA LTP kit (KAPA Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... Indexed Illumina sequencing libraries were constructed using the NEB Ultra Library Prep kit (New England Biolabs; Ipswich, MA).
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bioRxiv - Genomics 2023Quote: ... and further purified samples using a NEB Monarch® RNA Cleanup kit (New England Biolabs, Ipswich, MA, USA). We checked total RNA quantity and quality using a NanoDrop One spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... ZAP positive selection mutants were generated by the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) or synthesized as a gene block (Twist Bioscience ...
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bioRxiv - Genomics 2023Quote: ... RNA expression levels were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Sequencing library preparation was made using the NEBNext® UltraTM II DNA Library Prep Kit for Illumina (NEB) following the procedure described in “Library Prep for CUT&RUN with NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (E7645 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were constructed by NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB, Cat# E7530L), and were sequenced by Illumina NovaSeq 6000 sequencing machine with 150-bp paired-end reads by Beijing Novel Bioinformatics Co. ...
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bioRxiv - Cell Biology 2023Quote: ... pREP81-FLAG plasmid carrying wild-type srs2+ was constructed using the Gibson Assembly® Cloning Kit (NEB E5510S). After Gibson cloning ...
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bioRxiv - Cell Biology 2023Quote: ... Ribosomal RNA was depleted from 0.8 μg total RNA using NEBNext rRNA Depletion Kit (New England Biolabs, E6350L) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... nosP-5’Eno-GFP-3’Eno and nosP-5’Eno-GFP-3’SV40 plasmids were generated by assembly of PCR fragments into the pNOSPE_MCP_eGFP vector 63 digested by NotI and BamHI using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). pNOSPE_MCP_eGFP contains the nanos promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA libraries were prepared using the ‘NEBNext Ultra Directional RNA Library Prep Kit for Illumina’ (New England Biolabs). These samples were sequenced on the high-throughput sequencing platform (NovaSeq6000 ...
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bioRxiv - Bioengineering 2023Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... We prepared cDNA libraries from GFP+ and GFP-samples (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB) from RNA enriched with mRNA through bead-based polyA selection ...
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bioRxiv - Cell Biology 2023Quote: ... This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs) to create a new HindIII site at the position encoding amino acid 569 ...
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bioRxiv - Molecular Biology 2023Quote: ... The circularization of the plasmids was then achieved using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520). The primers utilized for site-specific mutagenesis are presented in Table S2 ...
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bioRxiv - Microbiology 2023Quote: PCR was carried out using NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, E3006) or Taqman Fast Universal PCR master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs E7760) and sequenced using a 150bp paired-end protocol on an Illumina Novaseq 6000 instrument ...
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bioRxiv - Molecular Biology 2023Quote: ... the positive control and each viral mutant plasmid was constructed using the Q5 site-directed mutagenesis kit (NEB) according to manufacturer protocol and verified via whole plasmid sequencing ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England BioLabs Inc. MA, USA) was used following manufacturers protocols for cDNA synthesis and amplification from 300 ng of RNA ...
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bioRxiv - Immunology 2024Quote: ... and poly-A enriched before library preparation using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, USA). For each library ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Duplexed oligonucleotides were ligated into dephosphorylated and BsmBI-digested pLB-Cas9 using the Quick Ligase kit (NEB M2200S) to generate transfer plasmid with RIPK1 guide sequence ...
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bioRxiv - Genetics 2024Quote: ... RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix (ThermoFisher Cat # A25741) ...
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bioRxiv - Developmental Biology 2024Quote: ... a pair of single guide RNAs (sgRNAs) were synthesized using the EnGen sgRNA Synthesis Kit (E3322V) from NEB; a total of 1μg of the sgRNA pair along with 20 pmol recombinant Cas9 (EnGen S ...
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bioRxiv - Microbiology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) and sequenced using a NextSeq 550 75 Cycle High Output kit for single-end sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). These libraries were subsequently sequenced for paired-end reads of 2×100 bp on an MGI system at a sequencing depth of 50X.
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bioRxiv - Molecular Biology 2024Quote: ... followed by cDNA library generation using NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to the manufacturer’s protocol and deep sequencing on Illumina HiSeq 2500 platform.
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bioRxiv - Genomics 2024Quote: ... Psoralen pull-down DNA was further purified using a Monarch PCR & DNA clean-up kit (New England Biolabs), eluted in 15 μl of EB buffer (10 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... Loss-of-function mutations were generated using site directed mutagenesis using Q5 Site-directed mutagenesis kit (E0552S, NEB) with the HA::SQSTM1/p62 plasmid as template (Addgene#280227) ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted from each pool using the Monarch® Total RNA Miniprep Kit (New England Biolabs) including on-column DNase treatment ...
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bioRxiv - Microbiology 2024Quote: ... Barcoded libraries were made with NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs). Libraries were pooled and sequenced (single-end 75-bp reads ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted using the New England Biolabs Monarch Total RNA Miniprep Kit (NEB #T2010, Ipswich, MA, USA). Acetone was removed from the tubes under the dissecting scope ...
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bioRxiv - Plant Biology 2024Quote: ... we used NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, catalog no. E7760L) protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Frozen samples were ground and RNA was extracted using the Monarch® Total RNA Miniprep Kit (NEB, UK). Equal amounts of RNA was used to synthesise cDNA with Revertaid cDNA synthesis kit (ThermoScientific ...
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bioRxiv - Plant Biology 2024Quote: ... sequencing libraries were fabricated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). The resulting libraries underwent sequencing on an Illumina Hiseq 4000 platform ...
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bioRxiv - Cancer Biology 2024Quote: ... The library was constructed using the NEB Next®Ultra™RNA Library Preparation Kit (New England Biolabs). The library quality was assessed using the Agilent Bioanalyzer 2100 system and then sequenced using the Illumina Novaseq platform ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Neuroscience 2024Quote: HA-tagged BirA-Tau fusion proteins were generated by using the NEBuilder™ HiFi DNA Assembly Kit (NEB). To this end ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were then prepared using the NEBNext Ultra II DNA library Prep Kit for Illumina (NEB, cat# E7645S). Two replicates were performed for each condition ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, E7420L). Library quality was assessed using Agilent 2100 bioanalyzer (High Sensitivity DNA Kit) ...
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bioRxiv - Microbiology 2024Quote: ... the PCR amplicons were purified used the Monarch PCR and DNA Cleanup Kit (New England Biolabs, Whitby, Canada) and sent for Sanger sequencing at the McMaster Genomics facility ...