Labshake search
Citations for New England Biolabs :
5951 - 6000 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: All plasmids were linearized with NotI and transcribed in vitro using the HiScribe(tm) T7 ARCA mRNA kit (NEB). mRNA was purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... and library preparation was conducted using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) according to manufacturer’s protocols ...
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bioRxiv - Biochemistry 2019Quote: peYFP-C1-PALB2 was modified to be resistant to PALB2 siRNA by Q5 Site-Directed Mutagenesis Kit (NEB, E0554) using primers JYM3892/3893 (Table S4) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10x diluted T7 RNA Polymerase mix (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs, USA). After linear amplification ...
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bioRxiv - Microbiology 2021Quote: ... and RNA-seq libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) as per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10x diluted T7 RNA Polymerase mix (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs, USA). After linear amplification ...
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bioRxiv - Plant Biology 2020Quote: ... 34 µL DNase-free water was added to 16 µL precipitated DNA and adaptors were ligated to DNA fragments according to the manufacturer’s instructions (NEBNext Multiplex Oligos for Illumina kit NEB). After ligation ...
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bioRxiv - Pathology 2021Quote: ... Fragmented PCR products were repaired and dA-tailed using the NEBNext End Repair/dA Tailing kit (New England Biolabs) according to the manufacturer’s instructions and subsequently barcoded with Illumina-compatible ...
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bioRxiv - Molecular Biology 2021Quote: ... Adaptor ligation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, New England Biolabs), and library pre-amplification was performed by 9 cycles of PCR ...
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bioRxiv - Genomics 2019Quote: ... We then cloned all three libraries into the pGL4.23c plasmid using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2017) using primers designed by NEBaseChanger and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs. Cat. No.: E0554S). The mutations were confirmed by DNA sequencing.
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR was conducted in technical triplicate using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s instructions in 10 µl reaction volumes and reactions were run on a CFX Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... THUNDERBIRD Probe qPCR Master Mix (Toyobo) for MERS-CoV and Luna Universal Probe One-Step RT-qPCR Kit (NEB) for SARS-CoV-2.
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bioRxiv - Microbiology 2020Quote: ... The Cas9/gRNA expression construct pTREX-n-Cas9 was first modified using the Q5 mutagenesis kit (NEB, Ipswich, Massachusetts), primers 5’-CCCAAAAAGAAAAGGAAGGTTGATTAGAAGCTTATCGATACCGTCGAC-3’ and 5’-GTCCTCGACTTTTCGCTTCTTTTTCGGGTCGCCTCCCAGCTGAGA-3’ ...
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bioRxiv - Genetics 2020Quote: ... DNA that migrated between the 400 and 600 bp was extracted using Monarch DNA Gel Extraction Kit (NEB T1020L). The pooled library DNA concentration was determined using Qubit dsDNA BR Assay Kit (ThermoFisher Scientist Q32853) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Each library was barcoded and sequenced in one lane of an Illumina HiSeq 2500 ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA for RNA and PARE sequencing was obtained by purification with the NEB mRNA isolation kit (New England Biolabs). RNA for sRNA library preparation was enriched with the mirVana miRNA Isolation Kit (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Strand-specific RNA-seq library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, Ipswich, MA) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: Illumina libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit (New England Biolabs, Frankfurt, Germany) following the instructions of the manufacturer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-seq libraries were constructed by NEBNext Ultra II RNA Library Prep kit (NEB, Cat: E7775, E7335, E7500) and sequenced by Illumina NextSeq 500 system (Duan et al. ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs, Frankfurt am Main, Germany) following the manufacturer’s description ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... ERK7-mAID-HA (TGGT1_233010) was obtained by transfecting a gRNA obtained by Q5 site-directed mutagenesis kit (New England Biolabs) on the pSAG1::Cas9-U6::sgUPRT vector (Shen et al ...
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bioRxiv - Immunology 2020Quote: ... Sequencing libraries were generated from sonicated DNA with the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). Ligation products were purified with SPRIselect beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... R972A mutations were introduced into the aforementioned IRS2 clones using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) with the primers 5’ - AGA TTA TAT GAA TAA GTC CAC TGT CAG ATT ATA TG - 3’ and 5’ - GAC AGT GGA CTT GCC TGG CGA GAG TCT GAA CT - 3’ according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: 5’-Phosphorylated linker (Oligonucleotide K, Supplementary Table 6) was adenylated using a 5’ DNA Adenylation Kit (New England Biolabs) at 20x scale and purified by TRIzol extraction as previously described56 ...
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bioRxiv - Microbiology 2019Quote: ... The pJ107106 promoter was converted to pJ107111 (Zucca et al., 2015) using the Q5 site-directed mutagenesis kit (NEB), with suggested protocols ...
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bioRxiv - Genomics 2021Quote: ... High-molecular weight DNA was extracted from blood with a Bionano Plant Tissue DNA isolation kit (Bionano Genomics) and digested with Nt.BspQI nickase (New England Biolabs). After labeling and staining ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760, New England Biolabs, Inc.). Paired-end sequencing (75 bp ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760, New England Biolabs, Inc.). Paired-end sequencing (75 bp ...
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bioRxiv - Genetics 2020Quote: ... TruSeq-barcoded RNA-seq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) and each library was quantified via Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Cell Biology 2020Quote: ... The libraries were constructed by using the NEBNext ultra II RNA library prep kit (New England BioLabs, Ltd., USA) and the qualities were analyzed with Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2021Quote: ... and enrichment using the NEBNext® Ultra™II DNA Library Preparation kit (New England Biolabs Inc, Ipswich, MA). Titration of the number of PCR cycles was performed as described in Belton et al ...
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bioRxiv - Genomics 2021Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Genomics 2021Quote: ... Illumina sequencing libraries were prepared using NEBNext rRNA Depletion and Ultra Directional RNA library prep kits (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: A library was prepared using New England Biolabs Ultra II DNA Kit for Illumina (New England Biolabs, United States) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We constructed the cDNA library using NEBNext®Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Bulk RNA sequencing libraries were constructed using the Nebnext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on a Nextseq 500 or Novaseq 6000 device ...
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bioRxiv - Biochemistry 2021Quote: ... 70 and 80 amino acids deletion mutants were carried out with the Q5 mutagenesis kit (New England Biolabs, MA). All AtAtm3 constructs were overexpressed in Escherichia coli BL21-gold (DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... Both fragments were run on a 1% agarose gel and purified using a Monarch DNA Gel Extraction kit (NEB). The fragments were then joined using the NEBuilder HiFi DNA Assembly protocol (NEB).
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bioRxiv - Plant Biology 2021Quote: ... The sequences were used to create libraries with NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).
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bioRxiv - Developmental Biology 2021Quote: ... The RNA libraries were constructed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina following manufacturer's recommendations and Oligo (dT) beads (NEB) were used to isolate poly (A ...
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bioRxiv - Immunology 2021Quote: ... The sequencing library was prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®(NEB) and xGen™ UDI-UMI Adapters (IDT ...
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bioRxiv - Biochemistry 2021Quote: ... c-Jun point mutations were generated by PCR-based mutagenesis using the site-directed mutagenesis kit (New England Biolabs). cDock ...
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bioRxiv - Microbiology 2022Quote: ... and selection marker of pSIN was replaced to match that of pMCB306 using the Gibson Assembly Cloning Kit (NEB) to generate the transfer plasmid (BAS2186 ...
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bioRxiv - Microbiology 2021Quote: Metagenomic shotgun libraries were prepared using New England BioLabs Ultra FS II library kit (New England BioLabs, Ipswich, MA) and cleaned with a 1:1 ratio of Sera-Mag speedbeads after ligation (Rohland and Reich ...
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bioRxiv - Biochemistry 2020Quote: ... Y85F and Y144F mutants of His6-MmfR were created using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Libraries were prepared for sequencing using the NEBNext Ultra II and Multiplex Oligios for Illumina kits (New England Biolabs) according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2019Quote: The CMV-EPO-eGFP-p2A-A1AT-mCherry construct was assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequences derived from PCSK9-eGFP-p2A-A1AT-mCherry (32 ...
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bioRxiv - Genetics 2021Quote: ... The latter was prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). Briefly ...