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Citations for New England Biolabs :
5701 - 5750 of 6282 citations for 6 Quinolinamine 1 ethyl 1 2 3 4 tetrahydro 2 2 4 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Twelve restriction enzyme digests were prepared by combining 15 µL of each purified DNA sample with 1 µL DpnI (NEB R0176S) and 4 µL 10X CutSmart Buffer (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... encompassing the targeted loci were generated using 1 μl of the zebrafish lysate with Phusion® High-Fidelity Polymerase (New England Biolabs) and primers listed in Supplemental Table S3 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was diluted 1:25 in water and used as template for RT-qPCR using the Luna Universal qPCR master mix (NEB M3003S). Primers used are listed in Supplementary Table 3 ...
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bioRxiv - Genomics 2022Quote: The 2.2 kb DNA was ligated with 1 kb biotin- and dig-labeled DNA through SbfI- and XbaI-overhangs by T4 DNA ligase (NEB Biolabs). The ligated products were attached to the anti-dig treated surface of reaction chamber and streptavidin-coated magnetic beads ...
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bioRxiv - Synthetic Biology 2022Quote: ... which was also double checked visually in an agarose gel where the density of target bands was compared with the DNA ladder (1 Kb Plus, NEB N0469S).
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bioRxiv - Microbiology 2022Quote: ... Purified RNA was quantified by ultraviolet spectrophotometry and 1 μg used for reverse transcription by LunaScript® RT SuperMix Kit (New England Biolabs), as described by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... A polyadenylated fraction was purified from the total RNA (1 µg) using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). This fraction was used to construct cDNA library using a previously described method [91] ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR reactions were performed with a final primer concentration of 1 mM using Q5® High-Fidelity 2X Master Mix (NEB). Equal volumes of X and Y PCR reactions were then combined ...
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bioRxiv - Plant Biology 2022Quote: ... Double promoter constructs were constructed by reamplifying the promoter region of interest with unique overhangs and cloning into the Level −1 universal acceptor backbones using the BsaI-HFv2 restriction enzyme (#R3733L New England Biolabs, USA). These parts were then assembled into the same Promoter + 5’ UTR acceptor backbone using the BpiI restriction enzyme (#ER1012 Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... PolyA RNA was then enriched from 1 μg of DNase I-treated RNA using Oligo d(T)25 Magnetic Beads (NEB S1419S), followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420) ...
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bioRxiv - Molecular Biology 2023Quote: The starting library strand (50 pmol) and regeneration hairpin (75 pmol) (Supp. Table 1) were combined in a 1X ligation buffer (New England Biolabs (NEB)) ...
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bioRxiv - Neuroscience 2023Quote: ... PCRs to confirm targeted and untargeted SMN2 loci were performed for each of the cell lines with primer pairs shown in table 1 using High-Fidelity 2X PCR Master Mix (NEB, M0541L) and Thermal Cycler C1000 Touch (Bio-RAD) ...
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bioRxiv - Genomics 2022Quote: ... 1 μg of gDNA was amplified in a 200 μl reaction using Q5® High-Fidelity 2X Master Mix (NEB #M0491). PCR master mix (100 μl Q5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Seven µL of DNA product was circularized in a reaction with 1 µL of 10X T4 DNA Ligase Reaction Buffer (NEB, #B0202A), 1 µL T4 Ligase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The 5’ ends of RNA fragments were phosphorylated by T4 PNK and ligated to 5’ adaptor (CGATCTCCAATTCCCACTCCTTTCAAGACCTrC) using T4 RNA Ligase 1 (NEB, M0437M). Ribosomal RNA (rRNA ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was incubated for 1 hour in the presence of an anti-SNAP-tag primary antibody (New England Biolabs #P9310S) diluted at 1:2000 in PBS + tween-20 0.1% ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were barcoded using Illumina i5 and i7 adaptors from NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) kit (New England Biolabs) and sequenced using a 150-cycle NextSeq 500/550 High Output Kit v2.5 with 75 forward and 75 reverse reads ...
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bioRxiv - Immunology 2024Quote: ... To subclone this pool we digested our protospacer-empty Libr.1-null transfer vector with BsmBI-v2 (New England Biolabs, NEB) overnight at 55 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was quantified by Nanodrop and 1 µg of RNA per sample was treated for ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (NEB, E6350) and then immediately followed by Library prep using NEBNext Ultra II kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 13000 x g for 1 minute and the supernatant was once more purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs). We expect the “crush and soak” method to improve signal strength if low labeling densities are used during extension.
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bioRxiv - Cell Biology 2024Quote: ... PCR amplicons were loaded on to 1% (w/v) agarose gels stained with Midori Green Advance DNA stain (Geneflow #S6-0022) alongside 100 bp ladder (NEB #N3231S), before submitting for DNA Sanger sequencing (Source Genomics ...
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bioRxiv - Microbiology 2023Quote: ... The expression plasmid was assembled via Gibson Assembly by mixing pETDuet-1 vector digested with NdeI and PacI with the gBlock in addition to Gibson Assembly Mix (NEB #E2611L) and incubating at 50°C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were amplified for N-1 cycles (being N the optimum Cq determined by qPCR reaction) using NEBNext High-Fidelity Polymerase (New England Biolabs, M0541). Libraries were purified with Sera-Mag Select Beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared with 0.1-1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalogue #E6560L) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The genomic region around rbpms2aae30 was amplified for 35 cycles with an annealing temperature of 60°C and the wild-type allele was digested with HaeIII for 1 hour (New England Biolabs, R0108S)2 ...
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bioRxiv - Developmental Biology 2024Quote: ... The surrounding tsc2vu242 genomic region was amplified for 35 cycles with an annealing temperature of 57°C and the wild-type allele was digested with HpyCH4IV for 1 hour (New England Biolabs, R0169S). Undigested and digested products were resolved in a 3% Metaphor 1:1 (Lonza)/agarose gel (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... The region around the miossa22946 allele was amplified for 35 cycles using 60°C for annealing and the mutant allele was identified by restriction enzyme digestion with DdeI for 1 hour (New England BioLabs, R0175S). The genomic region around the miossa20 allele was amplified for 35 cycles using 57°C for annealing and identified by restriction enzyme digestion of the wild-type allele with BsaWI for 1 hour (New England BioLabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... was synthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). For RNA-sequencing ...
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bioRxiv - Genomics 2024Quote: ... The digest product was run on a 1% agarose gel and the band was excised and purified using a Monarch DNA Gel Extraction Kit (NEB #T1020) following the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Microbiology 2024Quote: ... A fragment containing the CRISPR array plus the extra BsrG1 restriction site was cut from the synthesized pUC19 by digestion with DraI and BstZ17I enzymes and cloned into the BsaI-linearized pSTU-1 plasmid using the NEBuilder HiFi cloning kit (New England Biolabs, UK). The plasmid sequence was confirmed by sequencing (Eurofins Genomics ...
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bioRxiv - Genomics 2023Quote: A final Q5 PCR reaction was carried out for 8 cycles with the same formulation as PCR2 but using NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB, E7600S) according to manufacturer’s recommendations (65 °C annealing) ...
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bioRxiv - Microbiology 2023Quote: ... For the restriction digest 1 µg of genomic DNA from UACa20 and als4112Δ was incubated for 1 hour at 37 °C with the restriction endonuclease BamHI-HF (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2023Quote: ... TTP and IFN-λ1 were cloned using primers found in supplementary table 1 and inserted between pCW57-MCS1-2A-MCS2 EcoRI (NEB # R3101S) and BamHI (NEB # R3136S ...
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bioRxiv - Molecular Biology 2022Quote: ... Then the oligos went through PCR with KAPA HiFi HotStart Uracil+ Ready Mix and NEBNext Multiplex Oligos for Illumina Set 1 (NEB #E7600) as primers ...
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bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
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bioRxiv - Microbiology 2022Quote: ... the CPER product was subject to post-PCR nick sealing for 30 min at 50°C and 30 min at 60°C in a 25 μL reaction containing 1 mM β-nicotinamide adenine dinucleotide (NAD+) (NEB) and 0.5 μL HiFi Taq DNA ligase (NEB) ...
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bioRxiv - Genomics 2023Quote: ... the samples were incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were cleared by centrifugation at 214,743 rcf at 4°C in S55A rotor using a Sorvall Discovery M120 SE Micro-Ultracentrifuge and added to 1 mL of Chitin Resin (NEB # S6651S) in a Peirce centrifugation column (Thermo Fisher # 89897 ...
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bioRxiv - Biochemistry 2023Quote: ... Template DNA was digested with the addition of 1 μL RNase-free DNaseI (NEB # M0303S; enzyme stock at 2,000 U/mL) for 15 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the annealed substrates was 5′-end-labelled with [γ-32P]-ATP and T4 polynucleotide kinase (New England Biolabs). For fluorescently labeled HJ40 substrates ...
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bioRxiv - Microbiology 2023Quote: ... Annealed oligos were diluted 1:20and 2ul of annealed oligonucleotides was ligated into the digested pJMP8602 with T4 DNA ligase (NEB 0491) for either two hours at room temperature or 16 hours at 16°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB 5-alpha (fhuA2∆(argF-lacZ)U169 phoA glnV44 ϕ 80∆(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17) (New England Biolabs) was used for the initial two-plasmid assay ...
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bioRxiv - Neuroscience 2023Quote: ... (see the sequence map in Figure 1) was subjected to restriction digestion using two restriction enzymes: XbaI and AccI (New England Biolabs (NEB)) in the cutsmart buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... RNase-free DNase Buffer was added to samples until 1× final concentration together with 20 units of DNase I (NEB, M0303L) and incubated at 37°C for 20 minutes ...
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bioRxiv - Biophysics 2023Quote: ... we mixed the digested DNA constructs and handles in a 1:10 molar ratio and ligated them together using T4 DNA ligase (M0202, New England Biolabs, UK) at 16°C overnight ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using the NEBNext Ultra II RNA Library Prep kit with Sample Purification Beads (E7775S) and indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs E7600S). Library quality was assessed with the Agilent High Sensitivity DNA Kit (Agilent 5067-4626 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-5 μM SNAPf fused activator was incubated in a 1× PBS solution containing up to threefold excess of SNAP-surface 649 (New England BioLabs, #S9159S), 1 mM DTT ...
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bioRxiv - Immunology 2023Quote: ... The mRuby-OAS3 CDS and mRuby2 were amplified via PCR and primers (Supplemental table 1) and inserted into pLenti-PGK-PuromycinR via Gibson assembly (New England Biolabs: E2611S). The pLenti-mRuby2-PABPC1 is described in Burke et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and ScFv16 at room temperature for two hours in the presence of 25 mU ml-1 apyrase (NEB, cat. no: M0398S) and either C3a or EP54 for complex formation ...