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Citations for New England Biolabs :
5651 - 5700 of 6282 citations for 6 Quinolinamine 1 ethyl 1 2 3 4 tetrahydro 2 2 4 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The resulting fragment was then checked by 1% agarose gel and purified with the Monarch® DNA Gel Extraction Kit (New England Biolabs). The purified fragment was precipitated by ethanol precipitation and resuspended in buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR was performed with primers specific for target genes (see Supplementary Table 1 for the list of primers) using the Luna universal One-Step RT-qPCR kit (NEB; E3005). Experiment was performed using the QuantStudio3 (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... a colony of each isolate was resuspended in 50 µL of sterile water then 1 µL of resuspended bacteria was used in a PCR reaction using Phusion® High-Fidelity DNA Polymerase (New England Biolabs) with forward (16s-8F ...
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bioRxiv - Systems Biology 2020Quote: ... the samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Systems Biology 2020Quote: ... 10 mM DTT, 12% PEG 8000, 1 mM each dNTPs, 10 µM dN-SMRT oligo, 5 µl Klenow enzyme NEB #M0212) for 1 h at 37 °C ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with an additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Microbiology 2021Quote: ... and then subcloned into the directly downstream of PreScission protease site pGEX-6P-1 (Cytiva) by Gibson Assembly system (New England Biolabs Inc.) to create pRN108 ...
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bioRxiv - Microbiology 2020Quote: ... A band at approximately 1 kb was cut out and DNA extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the sgRNA cassette from lentiCRISPRv2 was removed by digestion with KpnI and EcoRI and the plasmid re-ligated using annealed repair oligo pair 1 (see Table S3) and Quick Ligation™ Kit (M2200L, NEB). Next ...
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bioRxiv - Plant Biology 2021Quote: ... deltoides xylem cDNA library (primers listed in Supplemental file 1) using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and cloned in a pENTR vector (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified by extraction from a 1% agarose gel using the Monarch DNA Gel Extraction Kit following manufacturer instructions (NEB # T1020S). The partially degenerate DNA oligonucleotide ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg of GST-beads were incubated for 15 min on ice in GRB buffer in a 25 μl reaction volume with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070) ...
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bioRxiv - Microbiology 2019Quote: ... nearly 1 kb upstream and downstream sequences flanking the bycA gene were PCR amplified (Phusion® High-Fidelity DNA Polymerase, NEB) using primers SL3 and SL4 ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified inserts and expression vectors pGEX-4T 1 and pMALTMc5X were digested with BamHI/SalI (for pGEX-4T 1 constructs) and NotI/PstI (for pMALTMc5X constructs) restriction enzymes (New England Biolabs, UK) and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... Biotin-labeled DNA fragments captured on beads were ligated to multiplexing adaptors by adding 5 μL each adapter (50 μM) and 1 μL T7 DNA ligase (NEB, M0318L) in 100 μL ligation mixture and incubating at room temperature for 1 hour with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... A reaction was carried out of the enzyme and its corresponding trisaccharide dissolved in 1X GlycoBuffer 1 (New England Biolabs, n.d.), deionized water ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μl of cells were used in a PCR reaction to obtain the gene for Sanger sequencing using Q5 (NEB, M0492). The PCR reaction was sent to Macrogen Europe B.V ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into pGex6P-1 in-frame with an N-terminal 3C-cleavable GST tag using HiFi assembly (New England Biolabs, USA).
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bioRxiv - Cancer Biology 2020Quote: ... 25uM RibOxi RT primer was then annealed to the reaction before ligation of 5’ RNA linker using T4 RNA ligase I at room temperature for 1 hour and subsequent reverse-transcription using Protoscript II (NE Biolabs, M0368S). cDNA was then purified with AmpureXP beads with 1:1.8 ratio ...
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bioRxiv - Microbiology 2020Quote: We tested four high-fidelity and/or long-range DNA polymerases in duplicate to evaluate improvements of read length during the amplification step: 1) NEBNext (NEB M0541), 2 ...
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bioRxiv - Immunology 2022Quote: ... then the single-stranded oligos (1 ng) were PCR amplified for 10 cycles with Q5 High-Fidelity DNA Polymerase (NEB, M0491L) using Oligo_Amp_F and Oligo_Amp_R primers to generate double-stranded DNAs ...
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bioRxiv - Genetics 2022Quote: ... run at 50 V for 18 hours at 4°C in Tris-acetate buffer at pH 8.0 with comparison to Quick-Load 1 kb Extend DNA ladder (NEB, Cat# N3239S). Unless otherwise stated ...
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bioRxiv - Microbiology 2019Quote: ... We added 1 mL of RAD reagent from the RAD002 kit and 0.2mL of Blunt TA ligase (New England Biolabs, MA, USA) and the reaction was incubated for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: Bacterial 16S rRNA genes were amplified from 50 ng DNA per sample diluted to 10 µL nuclease free water in 50 µL with 1 × LongAmp Taq master mix (New England Biolabs, Inc), 1 µL 16S rRNA gene barcoded primer (ONT-SQK-RAB-201 ...
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bioRxiv - Biochemistry 2019Quote: ... Immunoblotting was performed using HRP-conjugated anti-maltose binding protein (MBP) monoclonal antibody at 1:10000 dilution (New England Biolabs, #E8038) to verify equal expression levels of each construct.
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bioRxiv - Plant Biology 2019Quote: ... Expression of recombinant proteins was performed at 25°C for 4 h with 0,3 mM Isopropil-β-D-1-tiogalattopiranoside (IPTG) and affinity purification was obtained by using the amylose resin affinity matrix (New England Biolabs) in a buffer containing 60 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... A second adaptor was added to the cDNA by adding 50 pmol 5iLL-22 DNA adaptor (Table S6) and ligating with T4 RNA Ligase 1 (NEB, M0437M) at 23°C for 3 hrs ...
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bioRxiv - Molecular Biology 2020Quote: ... 46 separate 60 μl PCR reactions were performed with 1 μg gDNA in each reaction using Q5® High-Fidelity DNA Polymerase (New England Biolabs). For the zeocin treated sample the total amount of genomic DNA harvested was used ...
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bioRxiv - Microbiology 2020Quote: ... We prepared a library with a fragment size of 300 nucleotides and used the 10 μM NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335) lot 0091412 and the NEBNext multiplex Oligos for Illumina (Set 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μL of 10x SSS buffer and 1 μL of SSS Enzyme (NEBNext mRNA Second Strand Synthesis Module, New England Biolabs, USA) were added to the 17 μL of the purified sample ...
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bioRxiv - Microbiology 2020Quote: ... and run on an agarose gel in 0.5X Tris-borate-EDTA buffer at 70V alongside 1 Kb ladder (New England BioLabs, Ipswich, MA) or GeneRule Express DNA Ladder (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Genetics 2020Quote: ... was annealed with 1ul of the reverse sgRNA (100 uM) using 1 ul 10X T4 ligation buffer (New England Biolabs, NEB), 1 ul T4 PNK (NEB) ...
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bioRxiv - Genomics 2020Quote: ... 1 μM of NEBNext Unique Dual Index Primers and 25 μl NEBNext Q5U Master Mix (M0597, New England Biolabs, Ipswich, MA) were added to the DNA and amplified as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 pg of lhx1a:DsRed/pI-SceI plasmid DNA was injected into 1-cell stage embryos along with the I-SceI restriction enzyme (NEB, R0694S). These injected embryos were raised to adulthood and screened for DsRed expression in known lhx1a expression domains and isolated a stable Tg(lhx1a:DsRed ...
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bioRxiv - Biochemistry 2021Quote: ... at 37 °C for 1 hour or first with 5 units T4 Polynucleotide Kinase with 25 mM ATP in 1 X T4 Kinase buffer (T4PNK, NEB, M0236S) at 37 °C for 30 min and then with XRN-1 ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome remodeling reactions (25 μL) were carried out in REA Buffer with 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP ...
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bioRxiv - Synthetic Biology 2021Quote: Expression units were assembled from cloned level 0 parts into level 1 destination vectors provided by the MoClo kit using 40fmol of each vector and 20 U of BsaI-HFv2 (NEB, R3733L) with remaining conditions as described above ...
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bioRxiv - Synthetic Biology 2021Quote: ... Vector and miRNA target inserts were ligated at 1:10 molar ratio using 400 U of T4 DNA Ligase (NEB, M0202) using provided Ligase buffer in a reaction volume of 20 µL over night at 16 °C ...
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bioRxiv - Cell Biology 2020Quote: Crushed lungs were homogenized using a mechanical homogenizer (Omni International, Waterbuy CT) or cultured cells were lysed in 1× lysis buffer (NEB, #9803S) supplemented with complete protease inhibitors (Roche ...
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bioRxiv - Genomics 2020Quote: ... The PCR was carried out with 1 μl of Lambda DNA (20 ng/μl; Catalog number: N3011S. Vendor: New England Biolabs Inc), 12.5 μl of KAPA 2X Master Mix ...
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bioRxiv - Genomics 2019Quote: ... We tested nuclear DNA removal by starting with 1 µg of total DNA using either 10 or 20 Units of Exonuclease V (New England Biolabs; UK) and an incubation of 30 minutes or 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA of 1×108 blood stage was extracted for conventional PCR amplification with a Q5® High-Fidelity DNA polymerase (New England Biolabs) from the interference inserts corresponding to the sense and anti-sense fragments of a portion of 429 bp AKT-like genomic sequence of T ...
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bioRxiv - Immunology 2021Quote: ... in YPD supplemented with 100 µg/ml ampicillin and 1% glucose (YPD-Amp-Glu) were infected with M13KO7 helper phage (New England Biolabs, USA) at 7×109 plaque forming unit (PFU ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5 kb and 500 bp DNA fragments were placed in two independent lines along with another line for 1 kb Ladder (NEB, N232). Once bands were clearly identifiable ...
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bioRxiv - Genetics 2019Quote: ... Poly(A)+ RNA was selected from 1 μg of total RNA using the NEBNext Ultra Kit (New England Biolabs, Ipswitch, MA). Libraries were prepared and sequenced by Genewiz (South Plainfield ...
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bioRxiv - Cell Biology 2019Quote: ... The cDNA coding region corresponding to RDGB(USR1-LNS2)Δ (amino acids 1-472) was subcloned into pUAST-attB by using the restriction enzymes NotI and XbaI (NEB). Similarly ...
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bioRxiv - Genomics 2019Quote: To prepare the RNA sample for use in a smallRNA library preparation kit the sample was phosphorylated using 5 ul of 10X T4 PNK buffer and 1 ul of T4 PNK (NEB #M0201), 1 ul of SUPERASE-In ...
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bioRxiv - Immunology 2022Quote: ... The library for sequencing was prepared using 1 µg of RNA and the NEBNext Ultra kit (New England BioLabs, Ipswich, MA), according to the protocol for low-input samples ...