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Citations for New England Biolabs :
5601 - 5650 of 6282 citations for 6 Quinolinamine 1 ethyl 1 2 3 4 tetrahydro 2 2 4 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and the second linker SI183 5’-/Phos/NNAGATCGGAAGAGCGTCGTGTAGGGAAAGAG/ddC/-3’ was preadenylated by Mth RNA Ligase (New England Biolabs) as described previously8 ...
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bioRxiv - Microbiology 2023Quote: ... RNA and 3′ adapters were incubated at 22°C for 2.5 hr with 51 U of T4 RNA Ligase I (NEB) and 12 U of recombinant RNase inhibitor (Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified supernatant was then batch-bound for 3 hours on to 20 mL chitin resin (New England Biolabs) equilibrated with 200 mL of cell lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNAs were then dephosphorylated at their 3’ end using T4 Polynucleotide kinase (PNK, New England Biolabs, Cat: M0201) in MES buffer (100 mM MES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... The extracted chromatin was digested for 3 hours at 37 °C in a volume of 250.0 μL with 100 U of NlaIII (NEB) for 3C library preparation ...
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bioRxiv - Cancer Biology 2023Quote: ... adenosines were added to the 3′ ends of dsDNA and adapters were ligated (adapters from NEB, Ipswich, MA, USA). Following the adapter ligation ...
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bioRxiv - Cancer Biology 2023Quote: ... a DNA adapter (TableS6) was ligated to 3′ ends of nascent RNA using the T4 RNA ligase kit (NEB) by mixing 50 pmol adapter with 300-600 ng nascent RNA ...
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bioRxiv - Genetics 2023Quote: ... 100 ng of genomic DNA was digested with 5U of BtsCI in a 3 μl reaction (New England Biolabs), 1 hour at 50°C incubation and no enzyme denaturation ...
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bioRxiv - Microbiology 2023Quote: ... hysA (SAA6008_RS12255) and vigR 3’ UTR from JKD6008 were in vitro transcribed (IVT) using HiScribe T7 RNA polymerase (NEB). IVT products were RQ1 DNase treated (Promega ...
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bioRxiv - Microbiology 2023Quote: ... the 5’-PPP RNA in the total RNA was specifically capped with 3’-Desthiobiotin-GTP (New England BioLabs, N0761) by the Vaccinia Capping System (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: ... 5 µg of genomic DNA was dephosphorylated by 3 µL of Quick Calf Intestinal Phosphatase (CIP, New England Biolabs) in a total volume of 30 µL for 10 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: The 3’ ends of the ribosome footprint RNA fragments were treated with T4 polynucleotide kinase (New England Biolabs, M0201) to allow ligation of a pre-adenylated DNA linker with T4 Rnl2(tr ...
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bioRxiv - Cell Biology 2023Quote: ... and the newly formed CENP-A was pulse labelled with 3 μM SNAP-Cell® 647-SiR (S90102S, NEB), 2 h after release from the STLC block (early G1) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2020) and 5’Ncol1::mScarlet::3’Ncol119 plasmids using Gibson assembly according to the manufacturer recommendations (NEB, CAT#E2611). Sequences of Gibson primers used were:
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bioRxiv - Genomics 2024Quote: M13seq primer (5’-GACGTTGTAAAACGACGGC-3’) was labeled with 32P at 5’-end by T4 polynucleotide kinase (New England Biolabs). Primer/template (p/t ...
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bioRxiv - Neuroscience 2024Quote: ... incubated at 37°C for 12 minutes and then washed with 0.1 3 SSC buffer (Thermo, AM9770) supplemented with 0.05 U/ml RNase inhibitor (NEB, M0314L). RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42C using SuperScript II (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... 3 µL of the reverse phasing primer pool (100 nM) and 15 µL of Q5 Mastermix (New England Biolabs). Cycle conditions were 4 minutes at 98°C followed by 20x (30 seconds at 98°C ...
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bioRxiv - Molecular Biology 2024Quote: ... A customized linker (rand10_3Tr3) was added to the 3’ end of on-bead LAM products using T4 RNA ligase I (M0204S, NEB). Nested PCR was performed using indicated primer set (3Tr3-Adaptor_R and JM_F ...
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bioRxiv - Genomics 2024Quote: ... Two cycles of whole genome amplification were performed using 50 U of Klenow Fragment (3’→5’ exo-) (NEB, M0212M), dNTP solution mix (Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... A 7 mL fraction of the eluate was mixed with 3 mL of 6x purple gel loading dye (NEB) and loaded in 0.7-1% agarose gel with ethidium bromide ...
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bioRxiv - Immunology 2022Quote: ... Equal amount of total RNA in a maximal volume of 6 µ L is used for cDNA synthesis using NEB PhotoScript® First Strand cDNA Synthesis Kit (Catalog # E6300L, NEB Inc, Ipswitch, MA) as instructed in the manufacturer standard protocol ...
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bioRxiv - Biochemistry 2024Quote: ... A His-Tag encoding sequence was added to the C-terminus of RnfG by using oligonucleotides 5 and 6 via site-directed mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB, Frankfurt am Main, Germany). The resulting plasmid was checked by sequencing analysis (Microsynth Seqlab ...
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bioRxiv - Microbiology 2020Quote: ... was in-vitro transcribed from 1 μg of these templates using the HiScribe T7 High Yield RNA synthesis kit (NEB, E2040S). Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were resuspended in 200 µL DigWash with 1:100 pA-Dam (between 20-60 New England BioLabs units, Fig. S1B) and rotated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... libraries were prepared using the NEB Ultra II Library Preparation Kit and NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs). The generation of the libraries followed manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... 1μl XhoI digestion mix consisting of 5U XhoI restriction enzyme and 1 x NEB buffer 2.1 (New England Biolabs, Ipswich, Massachusetts), and 10 ng genomic DNA for iciHHV-6B samples or 200 ng DNA for non-iciHHV-6B samples.
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bioRxiv - Evolutionary Biology 2021Quote: ... We isolated poly-adenylated RNA from 1 ug of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and constructed sequencing libraries with the NEBNext Ultra II RNA Library Prep kit (NEB #E7770 ...
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bioRxiv - Developmental Biology 2020Quote: ... The mutant her9 allele carrying a 1 bp insertion was identified by RFLP due to the introduction of a BfaI restriction site (NEB: R0568S). Genomic DNA from whole embryos or from tail clips was extracted and amplified by PCR as described above ...
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bioRxiv - Developmental Biology 2020Quote: The mutant her9 allele carrying a 1 bp deletion was identified by RFLP analysis due to the introduction of a BsaJI restriction site (NEB: R0563S). The mutant her9 allele carrying a 1 bp insertion was identified by RFLP due to the introduction of a BfaI restriction site (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... Adaptor ligated RNAs 48-58nt long (corresponding to 19-29nt long input RNAs) were extracted and ligated to the 5’ adaptor using T4 RNA Ligase 1 (NEB, M0204). A total of ten variable nucleotides (unique molecular identifiers ...
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bioRxiv - Genomics 2020Quote: ... and 0.4 μl of 20 mg/ml BSA with 1 μl (10 U/μl) of T4 endonuclease V (NEB, T4-PDG) and 1 μl (10 U/μl ...
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bioRxiv - Neuroscience 2022Quote: ... donor plasmids were constructed by generating fragments using PCR from the indicated template with indicated primers in Data File 1 and assembled using NEBuilder HiFi DNA Assembly (NEB E5520S):
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bioRxiv - Neuroscience 2021Quote: ... total RNA (1 µg) was transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (Cat. No. E6560; New England BioLabs). Quantitative real-time PCR was performed with 20 ng of cDNA in a total reaction volume of 10 μL using Luna® Universal qPCR Master Mix (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis was performed from 1 µg total RNA using the ProtoScript® First Strand cDNA Synthesis Kit (New England Biolabs, NEB) with the provided random primer mix according to the suppliers’ instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was resuspended in 10x NEB CutSmart Buffer (8:1) and dephosphorylated by incubation with Quick calf intestinal phosphatase (CIP) (NEB, # M0525S) at 37 °C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: TCF20(1-1268)-mEGFP construct was generated from the TCF20-mEGFP plasmid using the Q5 Side-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Typhimurium NCTC 12023 and the vector including the Tet-on system aph tetR PtetA present on p4392 occurred using oligonucleotides as listed in Table S 1 and purified by PCR purification (NEB Monarch). The PCR product encoding for sadBA and the PCR product from vector p4392 were assembled by Gibson assembly according to manufactureŕs protocol (NEB Monarch) ...
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bioRxiv - Biochemistry 2020Quote: ... The CIP-treated RNA was then PNK-treated with 1 μL of 10× T4 polynucleotide kinase buffer (New England Biolabs, B0201S), 2 μL of [γ-P32]ATP (PerkinElmer ...
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bioRxiv - Microbiology 2019Quote: ... All fragments contained ~20bp overhangs and were assembled into EcoRV cut pLenti CMV Puro DEST (w118-1) using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) or Gibson-like master mix (Berkeley MacroLabs ...
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bioRxiv - Genomics 2019Quote: ... The amount of DNA was estimated by running an aliquot on a 1% Agarose gel along with a 1kb ladder (NEB#N3232s).
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bioRxiv - Microbiology 2019Quote: ... These fragments were cloned into the XhoI site of pACYCDuet-1 by a NEBuilder HiFi DNA Assembly kit (New England Biolabs, USA), resulting in the plasmid pEXT06 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were diluted to 0.1 OD595 in 1 mL of EMM5S containing 1 µM of silicon-rhodamine benzylguanine derivative SNAP-SiR647 (SNAP-Cell® 647-SiR, New England Biolabs). Culture tubes were wrapped in aluminum foil to protect them from light and incubated on a rotator overnight (about 15 hours ...
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bioRxiv - Microbiology 2019Quote: ... Eluted DNA was transferred to a new tube and PCR enrichment of the adaptor ligated DNA was performed using NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335). The PCR reaction mix was assembled according to manufacturers’ recommendations and placed in a thermal cycler set to the recommended conditions ...
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bioRxiv - Synthetic Biology 2019Quote: Fly genomic DNA was isolated in a pool by grinding in 25 µl of “Squish Buffer” (10 mM Tris, 1 mM EDTA, 25 mM NaCl, 8 U/ml ProK (NEB P8107S)) per adult ...
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bioRxiv - Genetics 2021Quote: ... barcodes and barcoding adapters (PCR Barcoding Expansion 1-96, EXP-PBC096, Oxford Nanopore Technologies, UK) by PCR using Q5 polymerase mastermix (NEB, USA) for individual fish identification according to manufacturer’s protocol in a 25μl reaction (98°C for 3 min ...
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bioRxiv - Genomics 2021Quote: ... 0.5 µg of the treated RNA was used for ligation to an RNA oligonucleotide with T4 RNA ligase 1 (NEB M0204) for 1 hr at 25 °C then converted to first strand cDNA with random priming and the ProtoScript II reverse transcriptase mix (NEB E6560) ...
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bioRxiv - Genomics 2021Quote: ... and a portion corresponding to 1 µg input RNA was converted to cDNA using the LunaScript RT SuperMix cDNA synthesis kit (NEB E3010). 1% of the cDNA was used for each qPCR reaction performed with the Luna Universal qPCR Mastermix (NEB M3003 ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were analyzed by SDS-PAGE and western blotting by using antibodies against MBP (1:1000, New England Biolabs, E8038S) or GST (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... fragments were generated by PCR from the indicated template with the indicated primers (Data File 1) and assembled using NEBuilder HiFi DNA Assembly (NEB E5520S). Plasmids were transformed into NEB competent cells (NEB C2987I) ...
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bioRxiv - Genomics 2021Quote: ... 2 μg genomic DNA from mESCs (both, with DSB induction and control without the induction) were digested using 1 μl 100 U/μL XbaI (NEB, #R0145T) and 4 μl 5 U/μL I-SceI (NEB ...