Labshake search
Citations for New England Biolabs :
5051 - 5100 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit by New England Biolabs (NEB) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
-
bioRxiv - Genetics 2022Quote: ... The sequencing library was prepared according to the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Specifically ...
-
bioRxiv - Genetics 2023Quote: ... RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix (ThermoFisher Cat # A25741) ...
-
bioRxiv - Immunology 2023Quote: ... The pEGIP-derived packaging vector pEGIP-hACE2 was generated by homologous recombination using the NEBuilder kit (NEB, E5520). The vector backbone was amplified using oligonucleotides with appropriate hACE2 sequence overhangs ...
-
bioRxiv - Molecular Biology 2023Quote: ... CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB #E7645L), and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
-
bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ul-tra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 µL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Concentrations of libraries were measured with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2023Quote: ... Library preparation was completed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using MiSeq v3 Paired End 150 bp (Illumina) ...
-
bioRxiv - Immunology 2023Quote: Site-directed mutagenesis of Unc93b1 was performed with the Q5 Site Directed Mutagenesis Kit (E0552S, New England Biolabs) according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2023Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Preparation Kit for Illumina (New England BioLabs), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... cahirinus animal using a Monarch HMW DNA Extraction Kit for Cells & Blood (T3050, New England Biolabs, Ipswich MA) following the manufacturer’s recommended protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... IP protocol described in (11) was followed thereafter and Epimark® N6-Methyladenosine Enrichment Kit (New England Biolabs) was used ...
-
bioRxiv - Bioengineering 2023Quote: ... GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs, Ipswich, MA). AAV-hSyn-hM3Dq-RAM-d2tTA was constructed as previously described19 ...
-
bioRxiv - Cell Biology 2023Quote: ... 1ng pcDNA3.3-OCT4 was added for the amplification using the Q5 High-Fidelity DNA Polymerase kit (NEB, M0491). pcDNA3-EGFP (addgene ...
-
bioRxiv - Genomics 2023Quote: Purified cDNA was used as an input for the Nextera XT DNA library preparation kit (New England Biolabs). Library quality and size was determined using a TapeStation (Agilent Technologies ...
-
bioRxiv - Developmental Biology 2023Quote: ... A library was prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... 50 µg of lysostaphin and 100 µl of the tissue lysis buffer (Monarch Genomic DNA purification kit, NEB). Cells were lysed at 37°C for 30 min and then processed following the manufactures instructions.
-
bioRxiv - Plant Biology 2023Quote: ... Amplified PCR products with appropriate expected sizes were purified with the Monarch® DNA Gel Extraction Kit (NEB). Purified PCR products were cloned into the pMiniT 2.0 vector (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... Hep3B cells and HBV control HepG2 2.2.15 cells and subjected to PCR amplification using the LongAmp Taq kit (NEB) and HBV primers spanning the CTCF binding motifs (HBV2 forward and Hep23B reverse) ...
-
bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from roughly 500,000 HEK293T cells using the Monarch Genomic DNA Purification Kit (NEB T3010L) and fragmented by digestion with XbaI and AccI to excise and release the genomic DNA region corresponding to the AAVS1 reporter ...
-
bioRxiv - Genomics 2023Quote: ... Sequencing libraries were prepared with NEBNext® Ultra II DNA Library Prep Kit for Illumina® (NEB #E7645). Note ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA extraction for other experiments was performed using the Monarch Total RNA Miniprep Kit (T2010S, New England Biolabs) according to the provided protocol.
-
bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from flash frozen samples with the Monarch® Genomic DNA Purification Kit (New England Biolabs), including the on-column RNase treatment ...
-
bioRxiv - Plant Biology 2023Quote: ... Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB) based on the domain boundaries reported in (28) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs, Ipswich, Massachusetts, USA).
-
bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra II Library Prep Kit for Illumina (New England BioLabs, #E7645S) per manufacturer’s instructions with minor modifications ...
-
bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
-
bioRxiv - Developmental Biology 2024Quote: ... The sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB Catalog# E7645S) following manufacturer instructions.
-
bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB). Pooled libraries were sequenced with paired end sequencing on a Novoseq 6000 by Novogene.
-
bioRxiv - Systems Biology 2024Quote: ... 400 ng RNA were used as input for NEBNext Ultra RNA Library Prep Kit (New England Biolabs, USA). One sample (nonlesional skin of Pat1 ...
-
bioRxiv - Microbiology 2023Quote: ... ZAP positive selection mutants were generated by the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) or synthesized as a gene block (Twist Bioscience ...
-
bioRxiv - Molecular Biology 2023Quote: ... three 25 μl PCR reactions were pooled and purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB), according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Micro-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) following77 manufacturer instructions and sequenced on the Illumina NovaSeq 6000 platform.
-
bioRxiv - Molecular Biology 2023Quote: ... The circularization of the plasmids was then achieved using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520). The primers utilized for site-specific mutagenesis are presented in Table S2 ...
-
bioRxiv - Genomics 2023Quote: ... RNA expression levels were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: Sequencing library preparation was made using the NEBNext® UltraTM II DNA Library Prep Kit for Illumina (NEB) following the procedure described in “Library Prep for CUT&RUN with NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (E7645 ...
-
bioRxiv - Microbiology 2023Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs E7760) and sequenced using a 150bp paired-end protocol on an Illumina Novaseq 6000 instrument ...
-
bioRxiv - Microbiology 2023Quote: PCR was carried out using NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, E3006) or Taqman Fast Universal PCR master mix (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2023Quote: ... and used to construct Illumina sequencing libraries with NEBNext Ultra Library DNA Prep Kit for Illumina (NEB, E7370). BrdU-labeled nascent DNA library fragments were then enriched by immunoprecipitation with anti-BrdU antibody (BD ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were constructed by NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB, Cat# E7530L), and were sequenced by Illumina NovaSeq 6000 sequencing machine with 150-bp paired-end reads by Beijing Novel Bioinformatics Co. ...
-
bioRxiv - Microbiology 2023Quote: ... Reverse transcription was carried out using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs #E6560S) with random hexamer mix ...
-
bioRxiv - Cell Biology 2023Quote: ... Ribosomal RNA was depleted from 0.8 μg total RNA using NEBNext rRNA Depletion Kit (New England Biolabs, E6350L) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA libraries were prepared using the ‘NEBNext Ultra Directional RNA Library Prep Kit for Illumina’ (New England Biolabs). These samples were sequenced on the high-throughput sequencing platform (NovaSeq6000 ...
-
bioRxiv - Genetics 2023Quote: ... The ints-4 sgRNA was inserted into plasmid pDD162 via the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). The sgRNA plasmid and repair template were Sanger sequenced to confirm correct construct assembly followed by microinjection into EG9882 strain with integrated Cas9 activity (10 ng µl-1 repair template ...
-
bioRxiv - Neuroscience 2023Quote: RNA-seq libraries were prepared with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), using 1 μg total RNA per experiment.
-
bioRxiv - Neuroscience 2023Quote: ... and then transcribed using pooled in vitro transcription (HiScribe T7 High Yield RNA Synthesis kit, New England BioLabs) and cleaned up with the NEB Monarch RNA Cleanup kit ...
-
bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England BioLabs Inc. MA, USA) was used following manufacturers protocols for cDNA synthesis and amplification from 300 ng of RNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... the positive control and each viral mutant plasmid was constructed using the Q5 site-directed mutagenesis kit (NEB) according to manufacturer protocol and verified via whole plasmid sequencing ...
-
bioRxiv - Biochemistry 2024Quote: The ATP binding mutants (APLFD11A, APLFR37A, APLFK95A) were generated using Q5® Site- Directed Mutagenesis Kit (NEB #E0554) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following the manufacturer’s protocols ...