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Citations for New England Biolabs :
4901 - 4950 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Complementary DNA (cDNA) was synthesized from 200ng RNA using LunaScript® RT-SuperMix kit (New England Biolabs #E3010). RT-qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs #M3003) ...
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bioRxiv - Developmental Biology 2022Quote: ... Y350A mutations of WW domains in Yki were generated by Q5 Site-Directed Mutagenesis Kit (NEB, Cat# E0554S) using pMT-yki-HA as a template ...
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bioRxiv - Genomics 2022Quote: ... The purified product was then in vitro transcribed using “HiScribe T7 High yield RNA Synthesis Kit (NEB, E2040S) and purified using Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... histolytica RNA was prepared using the Monarch Total RNA Miniprep Kit (NEW ENGLAND BioLabs, Ornat, Nes Ziona, Israel). According to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) as described above ...
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bioRxiv - Molecular Biology 2022Quote: ... and RNAseq libraries were prepared using the NEB Ultra II Directional RNA library Prep kit (New England Biolabs), with 1 ug total RNA input and initial poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 uL of Quick Ligation Buffer and 5 uL of Quick T4 Ligase (NEB Quick Ligation Kit, M2200S). The ligation reaction was incubated for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... viral cDNA was synthesized from the extracted RNA by using a LunarScript RT SuperMix Kit (New England BioLabs). The DNA was then amplified by performing a multiplexed PCR in two pools using the ARTIC-N5 primers and the Q5 Hot Start DNA polymerase (New England BioLabs ...
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bioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instruction and further purified using a Monarch® RNA Cleanup Kit (50 μg) (NEB). RNA integrity was monitored with agarose gels ...
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bioRxiv - Microbiology 2022Quote: ... A sequencing library was generated using the NEB Next® Ultra™ DNA Library Prep Kit (NEB, USA) following the manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Molecular Biology 2024Quote: ... and further cleaning (to get rid of DNA contamination) with Monarch® Total RNA Miniprep Kit (NEB, T2010S) by following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Ribosomal RNA was removed with NEB Ribo-Zero Plus rRNA Depletion Kit (New England Biolabs, Cat No. 20037135) prior to RNA-seq library preparation ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cDNA libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB #E7765).
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bioRxiv - Biochemistry 2023Quote: ... DNA synthesis was visualized by detecting biotin with the Phototope-Star detection kit (New England Biolabs Ref N7020S) and images acquired on a Chemidoc system (Biorad).
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bioRxiv - Developmental Biology 2024Quote: ... Template pDNA was linearized and ARCA-capped IVT-mRNA was produced using the T7 HiScribe kit from NEB, pseudo-uridine was incorporated into the reaction ...
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bioRxiv - Biochemistry 2024Quote: ... Mutants of PARIS were constructed from pFR85 using the Gibson method38 or Q5 site-directed mutagenesis kit (NEB). The PARIS triggers Ptr1 (T5 ORF094 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext ® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: Extracted RNA was treated with DNaseI and purified using Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The cDNA was synthesised from approximately 1000 ng of RNA using ReverTra Ace qPCR RT Master Mix with gDNA remover (TOYOBO ...
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bioRxiv - Physiology 2023Quote: ... The cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted using the Monarch® Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA). The extracted RNA was eluted in 50 μL of nuclease- free water and treated with Qiagen DNAse I (Germantown ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries for RNA-seq were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the NextSeq 500 platform (Illumina ...
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bioRxiv - Genetics 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina (E7760, NEB). The size of the libraries was determined using a Bioanalyzer ...
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bioRxiv - Genomics 2023Quote: ... Commercial and breeding program samples were extracted from frozen tissue using the Monarch Genomic DNA Purification Kit (NEB), as per manufacturer’ s instructions using the enzymatic cleanup protocol with both proteinase K and RNase A ...
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bioRxiv - Biochemistry 2024Quote: ... at 37°C for 30 min and purified by Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The concentration of crRNA was determined by Nanodrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and were sequenced on an Illumina NovaSeq 150bp paired-end platform ...
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bioRxiv - Genetics 2024Quote: ... RNA-Seq libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Capillary electrophoresis (Agilent Bioanalyser and Agilent Tapestation ...
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bioRxiv - Microbiology 2024Quote: ... non incorporated primers were removed using the Monarch PCR and DNA clean up kit (New England Biolabs, T1030), using a binding buffer ratio to DNA of 3:1 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product of the selected deletion mutant was purified using Monarch PCR and DNA cleanup kit (NEB) and sent for sequencing at Eurofins Genomics LLC (Louisville ...
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bioRxiv - Molecular Biology 2024Quote: ... the products were used to generate RNA transcripts using the HiScribe T7 In Vitro Transcription Kit (NEB, E2040S) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: Libraries were prepared using the NEBNext Ultra II FS Library Prep Kit (New England Biolabs, Ipswich, MA, USA), following the manufacturer’s instructions with inserts of approximately 350 bp ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs). Primers listed in supplemental table 10.
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bioRxiv - Bioengineering 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, US-CA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Immunology 2024Quote: ... Ligations into the respective backbone were performed overnight at 16°C using T4 DNA Ligase Kit (NEB, #M0202L). Ligations were transformed into NEB Stable E ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was either extracted with phenol-chloroform followed by Monarch Total RNA Miniprep kit (New England Biolabs) or with the Quick-RNA Miniprep kit (Zymo) ...
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bioRxiv - Immunology 2024Quote: ... Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). FLAG-tagged cDNAs were transferred into pTrip-CMV-Puro-T2A-RFP (72 ...
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bioRxiv - Genomics 2024Quote: ... The linearized vector was assembled with the amplified oligo pool using the NEBuilder HiFi DNA assembly kit (NEB) by mixing 50 ng of the linearized pIS-0 vector and 2 µL of the amplified oligonucleotide pool in a 20 µL reaction volume (Figure S2A) ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and mRNA was isolated and purified using the Magnetic mRNA Isolation Kit (New England BioLabs). Resulting purified mRNA was quantified using Nanodrop.
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bioRxiv - Neuroscience 2024Quote: ... CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB E7645L), and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... The two PCR products were extracted and assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). The assembly product was transformed into E ...
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bioRxiv - Neuroscience 2023Quote: ... column purified and ligated at a 1:1 vector to insert ratio using the QuickLig Kit (NEB #M2200S). The ASCL1 stop codon was subsequently mutated using the QuickChange II-XL site-directed mutagenesis kit (Agilent #200523 ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were completed with the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina” (NEB #E7805) and subsequently analysed on an Illumina instrument by following manufacturer’s protocols ...
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bioRxiv - Physiology 2024Quote: RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to manufacturer’s polyA mRNA workflow at the Advanced Analysis Center at the University of Guelph (Guelph ...
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bioRxiv - Neuroscience 2024Quote: One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England Biolabs) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA (100 ng) was used for cDNA synthesis using the ProtoScript II First Strand cDNA Synthesis Kit (NEB) with random hexamers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed with the NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, #E7645S) and sequenced on an Illumina HiSeq 3000 platform to generate a minimum of 20 million 150-bp reads per sample.
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bioRxiv - Genetics 2024Quote: ... Plasmids were then isolated using the Qiagen Miniprep kit and genotyped by digesting with BsrGI (New England Biolabs) to detect the presence of the TARS1 insert ...
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bioRxiv - Immunology 2024Quote: cDNAs were synthesized from isolated RNA samples using ProtoScript II First Strand cDNA Synthesis Kits (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for Illumina (Dual Index Primers ...