Labshake search
Citations for New England Biolabs :
5301 - 5350 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... pEGFP-C1 or pcDNA5/FRT/TO-N-TAP with gateway cloning using the SP6-CHIKV-replicon-SG-GLuc as a template. Mutants (except for replicon mutants, see above) were generated using standard mutagenesis procedures (e.g. Q5 mutagenesis kit (NEB)) and confirmed by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... SET8 domains as well as point mutations were performed using Q5 site-directed mutagenesis kit (New England Biolabs # E0554S). Primers used for cloning and mutagenesis are available upon request.
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were quantified using the NEBNext Library Quant Kit for Illumina (cat # E7630L, New England Biolabs, Ipswich, USA) and were pooled at equimolar ratios after size-adjustment ...
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bioRxiv - Cancer Biology 2020Quote: ... Briefly the key stages of this protocol included the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1μg total RNA (via the Magnetic mRNA Isolation Kit from NEB), a fragmentation step using divalent cations under elevated temperature to obtain approximately 300bp pieces ...
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bioRxiv - Cell Biology 2021Quote: 5ng of DRIP DNA was library prepped using the Ultra II DNA library prep kit for Illumina (NEB, E7645L) according to the manufacturer protocol using 6 PCR cycles ...
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bioRxiv - Cancer Biology 2020Quote: ... were developed using Q5 Site-Directed mutagenesis kit as per manufacturers protocol (New England Biolabs # E0554; Ipswich, MA, USA) and confirmed by sequencing(Z ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA-seq Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA) for Illumina as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Metagenomic libraries were prepared using the NEBNext Ultra II for DNA Library Prep kit (New England Biolabs, Ipswich, MA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... DNA ends were FFPE repaired and end-prepped/dA-tailed using the NEBNext FFPE DNA Repair kit (M6630, NEB) and the NEBNext Ultra II End-Repair/dA-tailing Module (E7546 ...
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bioRxiv - Cell Biology 2021Quote: The pIRESneo-EGFP-alpha Tubulin plasmid was obtained from Addgene (USA) and mutation (Y224G) of α-Tubulin were performed using a Q5 Site-Directed Mutagenesis kit (NEB #E0554S, USA). Hela cells were plated on six-well plates and incubated for 24 hours before transfected with these plasmids by Lipofectamine 2000 ...
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bioRxiv - Genomics 2021Quote: Sequencing libraries of total DNA were generated using the NEBNext Ultra DNA library prep kit for Illumina (NEB, USA) by Novogene (Beijing ...
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bioRxiv - Cell Biology 2021Quote: ... were purified with QIAprep spin miniprep kit (QIAGEN, Hilden, Germany, digested with restriction enzyme SacI-HF (NEB, Ipswich, MA), and served as templates for the KANMX and URA3 deletion cassettes ...
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bioRxiv - Genomics 2022Quote: ... and cloned into the STARR-seq plasmid with NEBuilder® HiFi DNA Assembly Cloning Kit (E5520S, New England Biolabs) per manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... Sequencing libraries were subsequently generated by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... mRNA was reverse transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (BioLabs, Ipswich, MA, USA). Ribosomal Protein L32 (RPL32 ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified DNA was indexed and amplified using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA).
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bioRxiv - Plant Biology 2022Quote: ... mRNA was reverse transcribed using the Protoscript II First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA, USA). qRT-PCR reactions were performed in LightCycler 480 96-well plates (Roche Diagnostics ...
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bioRxiv - Genomics 2022Quote: Four RNAseq libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The pLVX-StrepII-ORF-IRES-Puro plasmids were then cloned using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) from the pTriEX-6 plasmids ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNA generated using Protoscript II first-strand cDNA synthesis kit as per manufacturer’s protocol (New England Biolabs, USA). SARS-CoV-2 cDNA was subsequently amplified using ARTIC network v2 primers using two-step PCR amplification with Q5® High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... P301L/V337M)-EYFP plasmids after the Tau sequence using the Q5 site directed mutagenesis (SDM) kit (New England Biolabs). Tau fragments were subcloned into pcDNA3.1 by restriction digestion and further into pCW57.1- MAT2A all-in-one tet-off lentiviral backbone (a gift from David Sabatini (Addgene plasmid # 100521))71 by Gibson assembly ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) with oligo-dT priming according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The small RNA library was prepared using NEBNext Multiplex Small RNA Library Prep Illumina kit (New England Biolabs, USA) with some modifications (Plotnikova et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... pET32a-MaFRS2 and pET32a-MaFRSA were constructed from pET32a-MaFRS1 using a Q5® Site-Directed Mutagenesis Kit (NEB). Primers pRF31 & pRF32 ...
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bioRxiv - Cancer Biology 2022Quote: ... University Medical Center of the Johannes Gutenberg-University Mainz using the NEBNext Ultra Library Prep kit (New England Biolabs). For deep sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... Three sgRNAs designed against Rbms3 were cloned into the pLL3.3 sgRNA-CRE vector by modifying the original sgLKB1 plasmid with the Q5 Site-Directed Mutagenesis kit (NEB). The following sgRNAs against Rbms3 were used and pooled to make 2 lentiviruses.
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bioRxiv - Cell Biology 2022Quote: ... Purified RNA was used for RNA-seq library generation with NEBNext® Ultra™ RNA Library Prep Kit (NEB). Both the input sample without immunoprecipitation and the m6A IP samples were subjected to 150 bp paired-end sequencing on Illumina HiSeq sequencer ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR product was then gel purified with NEB Monarch® DNA Gel Extraction Kit (NEB, Cat No. T1020). Alongside pLentiCRISPRv2 (Addgene ...
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bioRxiv - Cancer Biology 2022Quote: ... The paired-end libraries were prepared using a NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... according to the instructions of an NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA). The purified library products were evaluated using the Agilent 2200 TapeStation system and Qubit 2.0 software (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Following amplification using Cell3™ Target: DNA Target Enrichment kit and NEBNext® Q5U® Master Mix (NEB, # M0597), DNA quality and quantity were measured using TapeStation 4200 system ...
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bioRxiv - Biochemistry 2022Quote: ... the indicated deletion and addition mutations were introduced by QuickChange site-directed mutagenesis (NEB, Q5 Site-Directed Mutagenesis Kit). For TatA variants in pBAD33 (a vector with arabinose-inducible araBAD operon ...
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bioRxiv - Molecular Biology 2022Quote: The strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries for next-generation sequencing were prepared using the NEBNext Ultra DNA Library Kit for Illumina according to the manufacturer’s protocol (New England Biolabs), and subjected to sequencing on Illumina Novaseq 6000 using PE150 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by library preparation using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB E7760). Quantification of the libraries was done by Qubit and TapeStation ...
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bioRxiv - Microbiology 2022Quote: ... while Gluc and Cluc expression levels were determined using Biolux Gaussia or Cypridina luciferase assay kits (New England BioLabs) and a microplate reader ...
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bioRxiv - Plant Biology 2022Quote: ... DNA libraries were prepared using the the NEBNext® Ultra™ DNA Prep Kit (New England BioLabs® Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2022Quote: ... 10 ng DNA was used to prepare sequencing libraries with the Ultra II DNA Library Prep kit (NEB #E7645S).
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bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared according to the manufacturer’s protocol in NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) and individually indexed with NEBNext Multiplex Oligos for Illumina (New England BioLabs) ...
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bioRxiv - Physiology 2022Quote: cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). 150bp reads of the cDNA libraries were generated on a NovaSeq SP (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Strand-specific reverse transcription was performed using 1 μg of RNA and strand-specific oligonucleotides (0.1 μM each) with the ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs) supplemented with actinomycin D (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... we quantified the libraries using the NEBNext® Library Quant Kit for Illumina (New England Biolabs, Ipswich, MA, USA), size-correcting for the average length reported in the Bioanalyzer report considering a 400-700 bp quantification window ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA was prepared in vitro by using the HiScribeTM T7 high–yield RNA synthesis kit (New England Biolabs) and purified using an RNeasy MinElute Cleanup kit (QIAGEN) ...
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bioRxiv - Microbiology 2022Quote: RNA sequencing libraries were prepared using the NEBNext Ultra II RNA library prep kit for Illumina according to the manufacturer’s instructions (NEB). Prior to library prep ...
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bioRxiv - Biochemistry 2022Quote: ... Mutants were created via side directed mutagenesis using primers designed on NEBaseChanger and Q5 site-directed mutagenesis kit following manufacturer’s directions (New England BioLabs). BL21(DE3 ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645S) with manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Libraries were prepared according to the manufacturer’s protocol in NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) and individually indexed with NEBNext Multiplex Oligos for Illumina (New England BioLabs) ...
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bioRxiv - Developmental Biology 2022Quote: ... truSeq-barcoded RNAseq libraries were generated with the Ultra II RNA Library Prep Kit (non-directional) (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Cell Biology 2022Quote: ... The fragments were assembled together with the SmaI-digested pBIG4MRH vector using the NEBuilder HiFi DNA Cloning kit according to the manufacturer’s instructions (New England Biolabs). The primers used are summarized in Table S7 ...