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Citations for New England Biolabs :
451 - 500 of 10000+ citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... DNA fragments were end-repaired and T-tailed with Klenow fragment lacking 5’ → 3’ and 3’ → 5’ exonuclease activity (NEB). In-house adapters containing 8-nucleotide unique molecular identifier (UMI ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (3 μg) was applied for poly(A) mRNA purification by using oligo-d(T) magnetic beads (S1419S, NEB). RNA fragmentation ...
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bioRxiv - Genomics 2019Quote: ... NEBNext Universal PCR primer for Illumina (5’-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC-s-T-3’) and NEBNext Index primer for Illumina (NEB, index# 9-13 for five libraries in each repeat) ...
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bioRxiv - Microbiology 2019Quote: ... The 2 resulting amplicons were cloned within the T-DNA of pPZP201BK digested with KpnI and BamHI through HiFi (New England Biolabs) assembly according to manufacturer’s instructions and recovered in Escherichia coli DH5α ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was submitted to mRNA Isolation and RNA fragmentation with NEBNext Oligo d(T)25 beads (NEW ENGLAND BioLabs) and cDNA synthesis ...
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bioRxiv - Microbiology 2021Quote: ... The insert possessing the additional A at 3’ end was ligated to the linearized vector with additional deoxythymidine (T) residues using T4 DNAligase (NEB). The plf gene cluster from strain QT598 (plfQT598 ...
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bioRxiv - Developmental Biology 2021Quote: ... a portion of the Hoxa13 gene including a part of the single intron and the remaining downstream part of the CDS was amplified from the axolotl genomic DNA and inserted into the vector pGEM-T along with a DNA fragment encoding T2a-mCherry-3xnls using Gibson assembly cloning (BioLabs). The resulting vector was injected into fertilised eggs along with the CAS9 protein (MPI-CBG ...
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bioRxiv - Microbiology 2022Quote: ... 5 µl of soluble lysate were incubated at 37 °C for 1 hour with 0.7 µM of a fluorescent ssDNA substrate (5′-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3′) and 2.5 U of UDG (NEB #M0280) in a total reaction volume of 10 µl (diluted in reaction buffer) ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 μg of total RNAs from 12-day-old Arabidopsis leaves were extracted using the Trizol method and incubated with 30 μl of pre-equilibrated oligo d(T)25 magnetic beads (NEB) for 15 minutes at room temperature with continuous rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... and mRNA fragments with a poly(A) tail were isolated by Oligo d(T)25 Magnetic Beads (NEB Biolab, S1419S). Illumina sequence adaptors were added during reverse transcription with SMRT methods (Wang et al. ...
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bioRxiv - Microbiology 2022Quote: ... The first and last 500 bp of the gene and the antibiotic cassette were then introduced in the pGEM-T Easy vector using HiFi DNA assembly (New England Biolabs) resulting in plasmids pLytA and pSpxB (Figure 9 ...
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bioRxiv - Microbiology 2023Quote: ... and eukaryotic mRNA was removed by binding and discarding the eukaryotic mRNA polyA region to oligo d(T)25 magnetic beads (England Biolabs). Finally ...
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bioRxiv - Immunology 2023Quote: ... mRNAs were enriched by incubation with Oligo d(T) Magnetic Beads (New England Biolabs, Cat. No. S1419S, Ipswich, MA, USA) and then fragmented/eluted by incubation at 94°C for 9 min ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then reverse crosslinked and lysed by adding 10μL of 10X Lysis-T (250mM EDTA, 2M NaCl, 10% Triton X-100) and 4μL of proteinase K (NEB, P8107S) and incubating at 55°C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: Polyadenylated RNA was purified from the DNase-treated total RNA using the oligo d(T)25 magnetic beads (NEB, S1419) and used for the library preparation ...
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bioRxiv - Plant Biology 2023Quote: ... We used 1 μg total RNA for mRNA purification using the NEBNext Oligo d (T) 25 magnetic isolation module (New England Biolabs), followed by first strand cDNA synthesis using NEBNext Ultra II RNA Library Prep Kit for Illumina according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... coli constitutive ribosomal promoter J23119 with a poly-T region directly downstream of the sgRNA sequence for transcription termination using KLD mutagenesis (New England Biolabs). pBR322 and pET28 expression plasmids were co-transformed into E ...
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Neuroscience 2021Quote: RNA was collected from cultured granule cells using the Monarch® Total RNA Miniprep Kit (NEB). Then cDNA was reverse transcribed using SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... iPS cells and their derivative clones using the Monarch Genomic DNA Purification Kit (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... and NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, E6420S). NextSeq 550 PE 75 HO (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed and RNA was isolated using the Monarch Total RNA Miniprep Kit (NEB T2010S), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was extracted from cell pellets using Monarch Genomic DNA Purification Kits (New England Biolabs) and viral episome copies quantified by qPCR of the viral gene ORF57 as described in the qRT-PCR method.
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bioRxiv - Cancer Biology 2023Quote: ... library preparation was performed using NEBnext single-cell/low input RNA library prep kit (E6420L, NEB). For NKX2.2 CRISPR experiments ...
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bioRxiv - Neuroscience 2023Quote: ... or the NEBNext Single Cell/Low Input RNA Library Prep Kit (New England Biolabs, Ipswich, MA) (corpuscles samples) ...
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bioRxiv - Cancer Biology 2023Quote: ... All single-cell libraries were prepared using the NEBNext Ultra II FS kit (New England Biolabs) following the manufacturer’s instructions but using one-fourth volumes ...
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bioRxiv - Cancer Biology 2023Quote: ... All single-cell libraries were prepared using the NEBNext Ultra II FS kit (New England Biolabs) following the manufacturer’s instructions but using one-fourth volumes ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from cells using the Monarch Total RNA Miniprep Kit (New England Biolabs), employing on-column digestion of residual DNA by DNAseI treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA library preparation using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB), and sequencing was performed at the Yale Stem Cell Center Genomics Core facility ...
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bioRxiv - Neuroscience 2023Quote: ... and HEK-293 cells were extracted using Monarch Total RNA Miniprep Kit (New England BioLabs, T2010S). To prepare RNA-Seq libraries ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the cell lysates using the Monarch Total RNA Miniprep kit (NEB T2010) and its quality was spot-checked for random samples using the Bioanalyzer High Sensitivity RNA Kit (Agilent 5067-1513) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from cells using the Monarch® Total RNA Miniprep Kit (New England Biolabs and 200ng of RNA was converted to complementary DNA (cDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... 3’UTRs were combined to the TagRFP-T CDS using the Gibson assembly Master Mix (Cat#E2611, New England BioLabs Inc). The resulting fragment was then amplified via PCR and digested prior ligation into a vector containing only Hofstenia promoter region ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified PCR products were ligated into the recombinant plasmid pZE0-P-MglE-T using the recommended standard USER cloning protocol (NEB, UK).
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bioRxiv - Cell Biology 2021Quote: ... 500ng-1 μg total RNA was enriched in poly-A tailed RNA transcripts by double incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and fragmented for 9 min at 94°C in 2X Superscript III first-strand buffer containing 10mM DTT (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... we hybridized λ-phage DNA with LAB07 and Lambda Poly-T oligos by thermal melting and subsequent ligation with T4 DNA Ligase (NEB, M0202) as previously described (41) ...
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bioRxiv - Biochemistry 2022Quote: ... and 4.5 μM biotinylated primer oligo IF238 (5/Biosg/TC TCC TCC TTC T) were annealed in T4 ligase reaction buffer (NEB B0202S). The mixture was heated to 75°C for 5 min and cooled to 4°C at a rate of −1°C min−1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 ng total RNA was enriched for poly-A tailed RNA transcripts by double incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and fragmented for 9 min at 94°C in 2X Superscript III first-strand buffer containing 10 mM DTT (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The mRNA was then isolated through two rounds of poly-A selection using the oligo-d(T)25 beads (NEB #S1419S). The RNA was digested with nuclease P1 (Wako USA #145-08221 ...
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bioRxiv - Cell Biology 2019Quote: ... 400 µL of lysate at the protein concentration of 1,5 µg/µL were added to 100 μL of washed Oligo d(T)25 Magnetic Beads (NEB, Ipswich, MA) and incubated at RT for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... depletion was achieved by taking the unbound supernatant after chRNA was incubated with the oligo-d(T) beads from the NEBNext Poly(A) mRNA magnetic isolation module (NEB, E7490L). Then ...
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bioRxiv - Plant Biology 2022Quote: ... PolyA RNA was then enriched from 1 μg of DNase I-treated RNA using Oligo d(T)25 Magnetic Beads (NEB S1419S), followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420) ...
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bioRxiv - Cell Biology 2023Quote: ... 5′-Phos-GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUU[Biotin-dT]U[Biotin-dT]UUACACTCTTTCCCTACACGACGCTCTTCCGATC∗T-3′[∗phosphorothioate bond]) was then ligated at the free DSB ends with the quick ligase enzyme (NEB; M2200). After the primer ligation ...
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bioRxiv - Plant Biology 2024Quote: ... and PE 2.0 (5′-CAA GCA GAA GAC GGC ATA CGA GAT CGG TCT CGG CAT TCC TGC TGA ACC GCT CTT CCG ATC* T-3′) for 15 cycles using Phusion polymerase (NEB M0530S). The library was purified by electrophoresis on a 1.2% agarose gel to get rid of adapter dimers ...
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bioRxiv - Genomics 2024Quote: ... Samples were then washed twice with PBS-T and incubated for 15 minutes with 20 µg/mL Streptavidin (New England Biolabs N7021S) and 100 µg/mL rAlbumin (New England Biolabs B9200S ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from 1 million cells using the Monarch Total RNA Miniprep Kit (NEB, #T2010S). We prepared cDNA from 1 μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB ...
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bioRxiv - Bioengineering 2020Quote: Genomic DNA was prepared from 1 million cells using the Monarch Genomic DNA Purification Kit (NEB, #T3010G). Diagnostic PCR was then carried out followed by gel extraction (NucleoSpin ...
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bioRxiv - Neuroscience 2020Quote: Libraries were prepared using the Next Single Cell / Low Input RNA Library Prep Kit (New England Biolabs). The quality and quantity of the final libraries were assessed with the TapeStation D5000 Assay (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: Libraries were prepared using the Next Single Cell / Low Input RNA Library Prep Kit (New England Biolabs). The quality and quantity of the final libraries was assessed with the TapeStation D1000 Assay (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from 1 million cells using the Monarch Total RNA Miniprep Kit (NEB, #T2010S). We prepared cDNA from 1μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB ...