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Citations for New England Biolabs :
451 - 500 of 1083 citations for Nucleoside diphosphate kinase A NDPKA Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... After end repair (T4 DNA polymerase, T4 DNA polynucleotide kinase, Klenow (all NEB) in the presence of dNTPs in ligation buffer (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Microbiology 2022Quote: ... the RNA 5′ ends were phosphorylated with T4 polynucleotide kinase (New England Biolabs). Adapters were ligated to the 5′ and 3′ ends of the RNA and first-strand cDNA synthesis was performed using M-MLV reverse transcriptase ...
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bioRxiv - Microbiology 2022Quote: ... Linear products were phosphorylated with T4 Polynucleotide Kinase (New England Biolabs, MA, USA) for 1 hr at 37°C and circularized with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... the linear PCR product was phosphorylated using T4 polynucleotide kinase (New England Biolabs) and ligated with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The annealed oligos were phosphorylated using T4 polynucleotide kinase (PNK, New England Biolabs). The phosphorylated and annealed oligos were then diluted in Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2022Quote: ... and 10 units T4 Polynucleotide Kinase (PNK) enzyme (New England Biolabs, cat#M0201S) for 2 hours at 37°C in 1X T4 PNK buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL was used in a 10 µL Kinase/Ligase/DpnI (KLD, NEB) reaction and incubated at room temperature for 10-20 min ...
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bioRxiv - Genetics 2023Quote: ... and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB). DNA was circularized at a concentration of 5 ng/μL with T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.75 µl 0.1 M DTT and 0.3 U/µl T4 Polynucleotide kinase (NEB), and then incubated at 37°C 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was phosphorylated for 1h at 37°C with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Genetics 2023Quote: ... The ICU11_sgRNA1_F/R oligonucleotides were phosphorylated using T4 polynucleotide kinase (New England Biolabs) and hybridized in a thermal cycler (Bio-Rad Laboratories T100) ...
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bioRxiv - Immunology 2023Quote: ... RNA fragments were then 3lll-dephosphorylated using T4 polynucleotide kinase (New England Biolabs) for 6 hours at 37°C in 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Hairpin oligonucleotides were phosphorylated by T4 polynucleotide kinase (New England Biolabs, Beverly, MA) followed by annealing at 100°C for 5min and cooling in the heat block for 3h ...
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bioRxiv - Biochemistry 2022Quote: ... were run and then phosphorylated and re-ligated using T4 polynucleotide kinase (NEB) and T4 ligase (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... 4 U/mL creatine kinase) containing 10 µMnt cssDNA (NEB, PhiX virion DNA) was supplemented with Rad51 (5 µM ...
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bioRxiv - Microbiology 2023Quote: ... 6 ng of sample was treated with 2 units T4 Polynucleotide Kinase (NEB) and incubated at 37°C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Oligo 1 and oligo 2 were annealed by T4 polynucleotide kinase (NEB, #M0201S) at 37 ℃ for 30 min and 95 ℃ for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was phosphorylated at the 5’ end by T4 polynucleotide kinase (NEB) and ligated to the 5’ adapter (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ ends were hydroxy-repaired by adding T4 polynucleotide kinase reaction mix (NEB) which was incubated for 45 minutes at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB). DNA was circularized at a concentration of 5 ng/µl with T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by end repair with T4 Polynucleotide Kinase (T4 PNK; NEB, Cat# M0201) at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Single strand DNA oligonucleotides were ligated using T4 Polynucleuotide Kinase (New England Biolabs) using manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: Ribosome-protected fragments were dephosphorylated using T4 Polynucleotide Kinase (M0201, New England Biolabs). Dephosphorylated RNA was extracted using ROTI Acqua P/C/I for RNA extraction (X985.1 ...
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Cancer Biology 2023Quote: ... cut with hi-fidelity EcoRI and BamHI (New England Biolabs), and gel purified ...
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bioRxiv - Biophysics 2019Quote: ... the 5’ ends of the PCR products were phosphorylated by T4 polynucleotide kinase (NEB), the products were purified and the free ends of the plasmid re-ligated by T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2021Quote: The RNA was then fragmented in 0.25X T4 Polynucleotide kinase (PNK) buffer (NEB M0201) (1 µl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The purified RNA was then treated with 20 units of T4 polynucleotide kinase (NEB) for 3h at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Oligonucleotides were radio-labeled with [γ-32P]ATP with the T4 polynucleotide kinase (NEB).
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bioRxiv - Microbiology 2021Quote: ... Fragments were then end-labeled with γ-32P-ATP using T4 polynucleotide kinase (NEB), separated on 15% TBE-Urea gels and visualized by autoradiography ...
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bioRxiv - Microbiology 2021Quote: ... The samples were dephosphorylated by incubating with 10 U of T4 Polynucleotide Kinase (NEB) for 1 hour at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Complimentary sgRNA oligos were phosphorylated with T4 polynucleotide kinase (New England Biolabs cat # M0201S) according to manufactures protocol and annealed to each other using a Bio-Rad C1000 thermocycler with the following reaction conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... act57B sequence: 5’-UCUUCCCCUC-3’ RNA oligonucleotides were end-labeled using T4 Kinase (NEB) with ATP [γ-32P]32 ...
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bioRxiv - Molecular Biology 2020Quote: ... The nucleic acids were subsequently radiolabeled with γ32P-ATP using T4 polynucleotide kinase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The 4mt-GCamP6f PCR product was phosphorylated using T4 Polynucleotide Kinase (New England Biolabs) and digested using AscI ...
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bioRxiv - Plant Biology 2022Quote: ... Probes were end-labelled using [γ-32P]ATP and polynucleotide kinase (New England Biolabs), according to (Sambrook et al ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by labeling with γ-P32-ATP and T4 polynucleotide kinase (Cat#: M0201L, NEB). The reactions were further processed and digested with P1 Nuclease (Cat# ...
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bioRxiv - Molecular Biology 2023Quote: ... Size selected RNA was dephosphorylated by T4 polynucleotide kinase (PNK, New England Biolabs, B0201S) treatment for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... T4- PNK (polynucleotide kinase) and NheI enzymes were purchased from New England Biolab (NEB).
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bioRxiv - Biochemistry 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
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bioRxiv - Cancer Biology 2022Quote: ... 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK) (NEB, M0201). The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the fragmented DNA was blunt-end repaired with T4 Polynucleotide Kinase (New England Biolabs) and then purified with the MinElute Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... cDNA was end repair and A tailed with T4 polynucleotide kinase (New England Biolabs). The sequencing library was prepared using SMRTbell Express Template Prep Kit 2.0 following the standard protocol ...