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Citations for New England Biolabs :
701 - 750 of 1083 citations for Nucleoside diphosphate kinase A NDPKA Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PM (5’-GACCCCGTTGACAGCG-3’) and PMrev (5’-TGGAACACCTGGCGGAAA-3’) with T4 polynucleotide kinase (0.075 U/µL) (New England Biolabs) and [g32P]-ATP (3000 Ci/mmol ...
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bioRxiv - Molecular Biology 2023Quote: ... pairs of complementary DNA oligos (table S1) were annealed and phosphorylated with T4 polynucleotide kinase [New England Biolabs (NEB)] as previously described (3) ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNAs were then dephosphorylated at their 3’ end using T4 Polynucleotide kinase (PNK, New England Biolabs, Cat: M0201) in MES buffer (100 mM MES-NaOH ...
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bioRxiv - Genomics 2023Quote: ... A mono-phosphate group was then added back to the 5’-end of RNAs by polynucleotide kinase (PNK, NEB) in the presence of 10mM ATP ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of immunopurified GST-tagged WRN fragment was phosphorylated in vitro by Casein Kinase II (New England Biolabs) in the presence of 1X NEBuffer (50mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... sense and antisense oligos containing the 20 bp guide target sequence wereannealed and phosphorylated with T4 Polynucleotide Kinase (NEB), then inserted between BbsI sites in the plasmid ...
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bioRxiv - Cancer Biology 2023Quote: Kinase-dead mutant (K37R) of Nek2A was derived from Nek2A in pJP1563 using Q5 Site-Directed Mutagenesis Kit (NEB) following the instructions of manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were subsequently resuspended in 20 μL dephosphorylation reaction mix (1X PNK buffer, pH 6.5, 5 units of T4 polynucleotide kinase (New England Biolabs), 0.5 μL RNaseOut Ribonuclease inhibitor (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was then extracted from washed beads using Trizol LS and treated with T4 Polynucleotide Kinase (New England Biolabs) to obtain 5’ phosphate ends for subsequent ligations and passed through NucAway columns (Ambion ...
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bioRxiv - Cell Biology 2023Quote: The 3’ ends of the ribosome footprint RNA fragments were treated with T4 polynucleotide kinase (New England Biolabs, M0201) to allow ligation of a pre-adenylated DNA linker with T4 Rnl2(tr ...
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bioRxiv - Molecular Biology 2024Quote: ... the total RNA of each sample (50 μg) was treated with 1 μL of polynucleotide kinase (New England Biolabs) in 50 μL of total solution at 37℃ for 6 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The crosslink bearing oligo and an uncrosslinked control was then phosphorylated by T4 polynucleotide kinase (New England Biolabs M0201S).
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bioRxiv - Cancer Biology 2024Quote: ... Forward and reverse primers (100 µM) were phosphorylated and annealed using T4 Polynucleotide Kinase (PNK; New England Biolabs M0201S) and 10x T4 Ligation Buffer (Thermo B69) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was dephosphorylated by incubation with 5 U T4 Polynucleotide Kinase in 1X T4 PNK Buffer (New England BioLabs) supplemented with SUPERaseIn RNase Inhibitor for one hour at 37°C and ligated to bar-coded linkers (NI-810 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). The enzymes mentioned were purchased from New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: C-terminally (His)6-tagged LukE was expressed in BL21 (DE3) Escherichia coli cells (NEB). Transformed cells were grown at 37 °C in Terrific broth supplemented with 100 μg/mL ampicillin to a density of OD600 = 0.6 ...
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bioRxiv - Immunology 2023Quote: In situ Hi-C experiments were performed as previously described2 using restriction enzyme MboI (NEB) with minor modifications ...
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bioRxiv - Genomics 2023Quote: ... pooled oligonucleotides were amplified via PCR using NEBNext 2X Hi-Fi PCR Master Mix (NEB) and primers:
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bioRxiv - Microbiology 2023Quote: ... His/Strep-Tag was cleaved using enterokinase according to the protocol of the manufacturer (NEB) and GlnA3Mtwas immediately purified from the digestion mix by size-exclusion chromatography as directed by the resin manufacturer (GE-Healthcare).
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bioRxiv - Cancer Biology 2024Quote: ... Hi-C libraries were treated with T4 DNA Polymerase (New England Biolabs, catalog No. M0203) to remove biotin from unligated ends ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Sheared and purified DNA were then treated with end repair enzyme mix (T4 DNA polymerase, Klenow DNA polymerase, and T4 polynucleotide kinase, NEB, Cat ...
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bioRxiv - Biophysics 2022Quote: ClpA pore-2 mutations were introduced using round-the-horn mutagenesis with T4 polynucleotide kinase and Q5 high-fidelity polymerase (New England Biolabs) into pET9a-M169TClpA ...
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bioRxiv - Genomics 2019Quote: ... RNA was resuspended and γ-ATP was added to the 5’ end of the RNA with T4 polynucleotide kinase (NEB) for 30min at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 pmoles of ssDNA were phosphorylated with 0.7 µl of [γ-32P]-ATP (7 µCi, Hartman Analytic) using T4 polynucleotide kinase (10 U, NEB). The reaction was supplemented with 10 mM DTT and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2021Quote: ... end repair was carried out with a mixture of bead-immobilized T4 DNA polymerase and T4 polynucleotide kinase (both kind gifts of Dr. M. Xu, New England Biolabs) in a 20 µl volume of End Repair buffer ...
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bioRxiv - Genetics 2020Quote: ... Oligo pairs were mixed at an equimolar ratio in PCR tubes containing T4 Ligation buffer and T4 polynucleotide kinase (NEB) for 5’ phosphorylation of oligos ...
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bioRxiv - Bioengineering 2021Quote: ... The Blunt End Ligation Method was used to construct the plasmid (Georgescu et al., 2003) with T4 DNA Ligase and T4 Polynucleotide Kinase (New England Biolabs). The sequence of the RBS of selected colonies was determined by submission of colony PCR products to Eurofins Genomics for DNA sequencing (Supplementary Table V) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was fragmented via ultrasound (4 pulses a 30 sec, 4 °C) and subsequently treated with T4 Polynucleotide Kinase (NEB). Half of the samples were then treated with terminator exonuclease (TEX ...
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bioRxiv - Microbiology 2021Quote: ... or beads alone were suspended in 1x NEBuffer™ for Protein Kinases with 200μM ATP (Thermo) and 100 units of CK2 (NEB) at 30°C for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: Protein bound glutathione Sepharose beads were resuspended in 1x NEBuffer™ for Protein Kinases supplemented with 1mM ATPγS and 10 units of CK2 (NEB) and incubated at 30°C for 45 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... Supplementary Table 1) were hybridized by slow cooling from 95-25°C and then phosphorylated using T4 Polynucleotide Kinase (NEB). The digested plasmid and the hybridized oligonucleotides were ligated using T4 Ligase (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... RJ-167-mer and RJ-5’Tail-167mer were radiolabeled with 32P at the 5′-end using T4 Polynucleotide Kinase (NEB). To generate the 3′ Tail and dsDNA DNA substrates ...
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bioRxiv - Molecular Biology 2022Quote: ... the sRNA fraction (∼15- 35nt) was isolated by gel extraction and RNA species bearing 5’-OH were monophosphorylated by T4 polynucletide kinase (NEB). This was followed by treatment by a terminator exonuclease (Epicentre) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-protein complexes were radioactively labeled with 32P-γ-ATP (20 μCi) using 40U T4 Polynucleotide Kinase (New England Biolabs) in supplied PNK buffer for 30 min at 37ºC ...
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bioRxiv - Biochemistry 2022Quote: ... 5’-phosphorylated DNA guides were either purchased from Integrated DNA technologies or generated by incubating an unmodified oligonucleotide with T4 Polynucleotide Kinase (NEB) at 37 °C for 30 minutes ...
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bioRxiv - Biophysics 2022Quote: Open complexes were pre-formed by incubating an excess of RNAP holoenzyme (>1.7 nM active) with <400 pM γ-32P-labeled promoter DNA (labeled with T4 polynucleotide kinase from NEB) in BB at 37 °C for at least 30 min ...
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bioRxiv - Molecular Biology 2020Quote: Gene-specific primers (Supplementary Table 4) were labeled with [γ-32P]ATP by phage T4 polynucleotide kinase (New England Biolabs), as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2019Quote: ... 10 pmol of oligonucleotide was labeled at the 5’ terminus with 0.05 mCi [γ-32P]-ATP using T4 Polynucleotide Kinase (New England Biolabs). For annealing ...
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bioRxiv - Biochemistry 2019Quote: ... GST fusion proteins of the CTD and its mutants CTD-AA and CTD-YA were phosphorylated by Cdc2 kinase (NEB) using 32P-γ-ATP for 30 minutes at 30°C before incubated with immunoprecipitated CoAA or hnRNP M ...
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bioRxiv - Molecular Biology 2019Quote: ... About 20picomoles of CIP treated RNA was 5’ end labeled with 50μCi of [γ-32P] ATP (10mCi/ mL, BRIT, India) by incubating with T4 polynucleotide kinase (New England Biolabs) at 37°C for 35 min ...
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bioRxiv - Cell Biology 2020Quote: ... The kinase-inactive mutant was generated by a two-step PCR method using the Phusion High-fidelity kit (New England Biolabs). In the first step ...
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bioRxiv - Molecular Biology 2020Quote: ... tRF-5s, itRFs were end-labeled with 32P-ATP (Board of Radiation and Isotope Technology, India) using polynucleotide kinase (NEB), purified through MicroSpin G-25 Columns (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’ ends of ds-probes were radioactively labelled with [ψ-32P]-ATP using T4 polynucleotide kinase (New England Biolabs), purified on Illustra Microspin G-25 columns (GE) ...
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bioRxiv - Synthetic Biology 2020Quote: Phosphorylate oligos by mixing 1-2 ul of each top-strand oligo along with 1x T4 ligase buffer and 1 ul T4 polynucleotide kinase (NEB). Polynucleotide kinase buffer will not work without supplementary ATP ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 pmol of the oligonucleotides 5’-ATTGTCATACCGATCCCAATTCGA-3’ and 5’-AAACTCGAATTGGGATCGGTATGAC-3’ were phosphorylated for 30 min at 37 °C using 1 mM ATP and 1 unit polynucleotide kinase (NEB) in the buffer supplied by the manufacturer and then hybridized in a thermocycler (5 min 95 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was then supplemented with 5 mM DTT and the same concentrations of T4 polynucleotide kinase (New England BioLabs) and [γ-32P]-ATP (PerkinElmer ...
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bioRxiv - Biochemistry 2021Quote: ... RNA (50 pmol) was radiolabelled at 5′ end with by using 10 units of T4 polynucleotide kinase (New England Biolabs) mixed to 3 μl of γ32P-ATP (3000 Ci/mmol 10 mCi/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Oligos including 20 bp sgRNA sequences plus appropriate overhangs were resuspended in ddH2O at 100 μM and annealed in T4 ligase buffer with T4 polynucleotide kinase (both NEW ENGLAND BIOLABS) starting from 95 °C for 5 minutes and then temperature was ramped down to 25 °C at 5 °C/min ...
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bioRxiv - Microbiology 2022Quote: ... Radioactive probe was prepared using 40 pmol of primer 59 and 5’-labelled with 10 U of T4 Polynucleotide Kinase (New England Biolabs) and [γ32P]ATP (150 μCi) ...
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bioRxiv - Cancer Biology 2022Quote: ... pDONR223-SGK used as a template to generate the myr SGK1 construct as well as SGK1 kinase dead (K127M) constructs using Site-Directed Mutagenesis (New England Biolabs). SGK1 was mutated with the following primers ...