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Citations for New England Biolabs :
251 - 300 of 1083 citations for Nucleoside diphosphate kinase A NDPKA Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 4 μL T4 polynucleotide kinase (New England BioLabs) in a 100 μL reaction for 2 hours and purified using P-30 spin columns (BioRad) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5’ end-labeled using T4 polynucleotide kinase (NEB) and [γ-32P] ATP ...
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bioRxiv - Biochemistry 2022Quote: ... followed by KLD (Kinase, Ligase, and DpnI) digestion (NEB). Detailed primers used for TatA variant construction can be found in Supplemental Table 2 ...
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bioRxiv - Cell Biology 2022Quote: ... T4 Polynucleotide Kinase (1:10, #MO201S, New England Biolabs), T4 Ligase reaction buffer (#B0202S ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and phosphorylated using T4 polynucleotide Kinase (New England Biolabs). The phosphorylated DNA was cleaned and concentrated again as above and then ligated using T4 DNA Ligase (New England Biolabs) ...
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bioRxiv - Biophysics 2019Quote: ... and T4 polynucleotide kinase were also purchased from NEB. All other chemicals were purchased from Sigma-Aldrich (St ...
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bioRxiv - Molecular Biology 2019Quote: ... [γ32P]ATP and T4 polynucleotide kinase (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... The reverse primers are phosphorylated using Polynucleotide kinase (NEB).
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bioRxiv - Microbiology 2021Quote: ... 1 µl 10× T4 polynucleotide kinase (PNK) buffer (NEB), 6 µl of water ...
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bioRxiv - Cancer Biology 2021Quote: ... Insert oligonucleotides were phosphorylated using T4 polynucleotide kinase (NEB) at 37 °C for 1 hr and were annealed by heating to 95°C for 5 min and slowly cooled to room temperature ...
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bioRxiv - Pathology 2021Quote: ... then treated with Kinase-Ligase-DpnI (New England Biolabs) prior to heat shock transformation into chemically competent DH5α Escherichia coli cells (Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... T4 polynucleotide kinase (New England Biolabs, with 1mM ATP) and Terminal exonuclease (buffer A ...
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bioRxiv - Genetics 2019Quote: ... Annealed oligos were phosphorylated by T4 polynucleotide kinase (NEB) for two hours at 37°C followed by 65°C for 20 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... T4 Polynucleotide kinase PNK 200 U/ml (NEB M0201L); DNA Polymerase I Large (Klenow ...
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bioRxiv - Cell Biology 2022Quote: ... Oligos were phosphorylated with T4 Polynucleotide Kinase (NEB M0201) at 37oC for 30 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... Following phosphorylation with T4 polynucleotide kinase (New England BioLabs), the PCR products were ligated into expression vector pQE-1 (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’ phosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to a blunt-end linear vector (typically also PCR products) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were circularized with T4 kinase (NEB, M0201L) and T4 ligase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... DpnI restriction enzyme and polynucleotide kinase were used (NEB). Some constructs were made using the Gateway cloning system (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 7 μl Polynucleotide Kinase (10 U/µl; NEB) in a total of 100 μl and incubation for 20 minutes at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by KLD (Kinase, Ligase, and DpnI) digestion (NEB) by using primers deltaTatA_F (5’-GTGTTTGATATCGGTTTTAGC-3’ ...
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bioRxiv - Biophysics 2023Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL T4 polynucleotide kinase (PNK; New England Biolabs), 1 μL DpnI (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... These primers were phosphorylated using T4 polynucleotide kinase (NEB) prior to PCR ...
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bioRxiv - Neuroscience 2023Quote: ... and were then phosphorylated using T4 polynucleotide kinase (NEB) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 5’ phosphorylation with T4 Polynucleotide Kinase (NEB) and self-ligation with T4 DNA Ligase (NEB).
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bioRxiv - Biophysics 2023Quote: ... The sample was phosphorylated using protein kinase A (NEB) and then further purified on size exclusion chromatography.
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bioRxiv - Cancer Biology 2023Quote: ... RNA was 3’ dephosphorylated using T4 Polynucleotide Kinase (NEB) and preadenylated linkers were ligated to the RNA fragments using T4 RNA Ligase 2 K277Q (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... and end-labeled using T4 polynucleotide kinase (M0201, NEB) and [γ-32P] ATP (NEG002A250UC ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 poly nucleotide kinase (New England Biolabs, M0201L) and then ligated with 3ʹ RNA linker (with sample barcodes ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
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bioRxiv - Cancer Biology 2020Quote: ... with Hi-Fi DNA builder (NEB). Primers used to amplify specific regions are described in (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified small RNA samples were then digested using Nucleoside Digestion Mix (catalog no. M0649S, New England BioLabs, Beverly, MA). LC-MS/MS measurement of various post-transcriptional modifications were performed on a Waters XEVO TQ-STM (Waters ...
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bioRxiv - Synthetic Biology 2021Quote: ... the IVT template RNA (0.01– 0.05 A260 units) was digested into nucleosides using 10 µg/ml nuclease P1 (New England Biolabs, M0660S), and 0.5 U/ml Bacterial Alkaline phosphatase (Takara 2120A ...
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bioRxiv - Molecular Biology 2022Quote: ... 2019 [36] with the modification that 3 µg of gDNA per technical replicate were digested using Nucleoside Digestion Mix (NEB M0649S ...
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bioRxiv - Biochemistry 2020Quote: ... NC-siRNA sense: 5’-AGGUAGUGUAAUCGCCUUGdTdT-3’.35,36 NEBuffer™ 2 and nucleoside digestion mix were obtained from NEB (Ipswich, MA). Adenosine and N6-methyl adenosine (m6A ...
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bioRxiv - Systems Biology 2019Quote: ... using the same method as the LC-MS/MS method described below for Dimroth rearrangement analysis following hydrolysis into nucleosides by RNA 5’ pyrophosphohydrolase (RppH, NEB) and shrimp alkaline phosphatase (SAP ...
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bioRxiv - Molecular Biology 2022Quote: Purified modified RNA and RNA without any chemical modification were digested with the Nucleoside digestion mix (M0649, New England Biolabs) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Total amount of 100 ng of small RNA or mRNA was digested with a Nucleoside Digestion Mix (New England BioLabs) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... Exchange of V5/His to myc/His was performed using the Q5 site-directed mutagenesis kit (NEB #E0554) according to the manufacturer’s protocol resulting in pEF1-ZAP-S-myc/His and pEF1-ZAP-L-myc/His ...
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bioRxiv - Biophysics 2021Quote: ... The E1371Q samples were phosphorylated using protein kinase A (NEB). The wild-type sample was de-phosphorylated using λ-phosphatase ...
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bioRxiv - Genetics 2021Quote: ... and 10 U polynucleotide kinase (New England Biolabs, Beverly, MA) as instructed by the manufacturer ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... and then modified using T4 Polynucleotide Kinase (New England Biolabs), which removes the 3’-phosphate and adds 5’-phosphates ...
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bioRxiv - Genetics 2019Quote: ... followed by treatment with T4 polynucleotide kinase (New England BioLabs) as described previously53 ...
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bioRxiv - Cancer Biology 2020Quote: ... annealed oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, M0201S) and cloned into BsmBI-linearized ...
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bioRxiv - Genomics 2019Quote: ... the RNA products were treated with Polynucleotide Kinase (PNK, NEB) at 37°C for 30 min.
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bioRxiv - Molecular Biology 2020Quote: ... Telomere probe was labeled using T4 polynucleotide kinase (NEB M0201) and <ι>γ-P32-ATP (Perkin Elmer NEG035C ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with 8 μl of T4 Polynucleotide Kinase (NEB) and 2 μl of 10 mM ATP for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the oligos were phosphorylated by T4 Polynucleotide Kinase (M0201S, NEB) at 37°C for 30 min ...