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Citations for New England Biolabs :
451 - 500 of 10000+ citations for Mouse T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... mRNAs were incubated with Oligo d(T) Magnetic Beads (New England BioLabs S1419), then fragmented in 2x Superscript III first-strand buffer (Thermo Fisher Scientific with 10mM DTT (Thermo Fisher Scientific 18080044 ...
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bioRxiv - Genomics 2022Quote: ... mRNA was isolated using Oligo d(T)25 Magnetic Beads (New England Biolabs) and fragmented ...
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bioRxiv - Genomics 2022Quote: ... mRNAs were enriched by incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and then fragmented/eluted by incubation at 94°C for 9 min ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... mRNA molecules are captured using magnetic Poly(T) beads (NEB, catalog no. E7490L) following purification ...
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Plant Biology 2023Quote: ... and terminator (269 bp downstream) was polymerase chain reaction (PCR)–amplified from K60 gDNA and cloned via Gibson Assembly (New England Biolabs, Ipswich, MA, USA) at the HindIII site of pUC18miniTn7Gm to create pUC18miniTn7Gm::ripUK60 ...
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bioRxiv - Microbiology 2023Quote: ... followed by the second DNA chain synthesis using NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Ipswich, MA, USA). The obtained cDNA was used to prepare a NEBNext DNA Library Prep Set for Ion Torrent (New England Biolabs ...
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bioRxiv - Synthetic Biology 2019Quote: ... A FLAG surface tag was C-terminally appended to MxEnc and QtEnc using Q5® Site-Directed Mutagenesis (New England Biolabs). QtEncFarn was generated by appending a minimal C-terminal farnesylation signal (GCMSCKCVLS ...
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bioRxiv - Cell Biology 2019Quote: ... were performed with 5-10 μM final protein concentration and 10 μM dye (SNAP-Surface Alexa Fluor488 (New England Biolabs S9129S) or SNAP-Surface Alexa Fluor647 (New England Biolabs S9136S) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-5 μM SNAPf fused activator was incubated in a 1× PBS solution containing up to threefold excess of SNAP-surface 649 (New England BioLabs, #S9159S), 1 mM DTT ...
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in spheroblast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 μM beta-mercaptoethanol) and digested with 0.002% 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were resuspended in 1 mL spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 µM beta-mercaptoethanol), and 1.2–5 µL 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Molecular Biology 2019Quote: ... Polyadenylated transcripts were purified using oligo d(T)25 magnetic beads (New England Biolabs). RNA was dephosphorylated using FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: PBMCs were co-emulsified with oligo d(T)25 magnetic beads (New England Biolabs) in lysis buffer (100mM Tris pH 7.5 ...
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bioRxiv - Genetics 2022Quote: ... which cuts if the alternate T allele is present or with Msc1 (NEB R0534), which cuts if the C allele is present ...
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bioRxiv - Genomics 2022Quote: ... Illumina sequencing adapters containing uracil hairpin loop structure and 3′ T overhangs (NEB, #E7337A) were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... samples were incubated with 20 μl Oligo d(T)25 Magnetic Beads (NEB, S1419S) in PCR strips for selection of polyadenylated (poly(A) ...
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bioRxiv - Genomics 2024Quote: ... Poly(A) mRNA was selected using Oligo d(T)25 Magnetic Beads (NEB, S1419S) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: PBMCs were co-emulsified with oligo d(T)25 magnetic beads (New England Biolabs) in lysis buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was depleted of ribosomal RNAs using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... sequence of iRFP670 amplified from piRFP670-N1 vector by polymerase chain reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) and primers encoding NcoI and XmaI digestion sites (Supplementary Table S1) ...
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bioRxiv - Biophysics 2021Quote: ... The surface is then passivated by incubating bovine serum albumin (BSA, New England Biolabs, 10 mg/ml in PBS and 50% glycerol) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The kinesin and GBP constructs were exchanged into 1x PBS with 1 mM DTT and labeled with DNA and SNAP-Surface Alexa Fluor 647 (NEB, Ipswich, MA) dye directly after elution ...
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bioRxiv - Molecular Biology 2022Quote: For chitin-based sandwich ELISA 50 μl of chitin magnetic beads (New England Biolabs, Ipswich, MA, USA) were transferred into a 1.5 ml reaction tube ...
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bioRxiv - Synthetic Biology 2023Quote: ... The Python script by default selects codons with the highest usage frequency for the input organism (E. coli NEB 10-Beta in this study), unless specified problematic sequences are introduced (Supplementary Figure S3B) ...
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bioRxiv - Developmental Biology 2024Quote: ... NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB #E6420) was used for cDNA synthesis ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit (NEB #E6420) by following manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... in conjunction with the NEBNext® rRNA Depletion Kit for Human/Mouse/Rat (New England BioLabs, Ipswich, MA, USA) and the MICROBExpress kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... elegans and mouse cDNA (PolyATtract® mRNA Isolation Systems, Promega and ProtoScript® First Strand cDNA Synthesis Kit, NEB). Detailed sub-cloning information is available upon request.
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bioRxiv - Cell Biology 2022Quote: mRNA was enriched from 100ng DNase treated total RNA using the NEBNext rRNA depletion Kit (human, mouse, rat, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A 140-500 ng aliquot of total RNA was rRNA depleted using NEB’s Human/Mouse/Rat RNAse-H based Depletion kit (New England BioLabs). Following rRNA removal ...
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bioRxiv - Microbiology 2021Quote: ... poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (NEB) that were subsequently fragmented into shorter mRNA fragments using divalent cations under an elevated temperature (RNA fragmentation reagents kit ...
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bioRxiv - Biochemistry 2023Quote: The exonuclease T degradation assays were performed as described by the manufacturer (New England BioLabs). Reactions (20 μl ...
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bioRxiv - Cell Biology 2023Quote: ... the PB-T-PAF vector was linearized with NheI and NotI-HF restriction enzymes (NEB). PH-FFAT/N-PH-FFAT sequences (5’-NheI/3’-AscI ...
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD-SNAP was biotinylated or fluorescently labelled by overnight incubation at 4°C with SNAP-Biotin® or SNAP-Surface® Alex Fluor® 647 (New England BioLabs) with at least a 3:1 molar excess of these labels to PfK5ΔL6-MD-SNAP ...
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bioRxiv - Microbiology 2023Quote: ... Hep3B cells and HBV control HepG2 2.2.15 cells and subjected to PCR amplification using the LongAmp Taq kit (NEB) and HBV primers spanning the CTCF binding motifs (HBV2 forward and Hep23B reverse) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: N-linked glycans were enzymatically released from purified mouse THP using PNGase-F kit (catalog no P0709S, New England Biolabs). N-glycans were then purified from the reaction mixture containing denaturing buffer and de-N-glycosylated proteins by solid phase extraction method using Sep-Pak C18 (1 cc Vac-cartridges ...
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bioRxiv - Microbiology 2024Quote: ... AnTat1.1 sequence was obtained from AnTat1.1 specific cDNA from mouse infection D6 cloned into a pMiniT vector with the PCR Cloning Kit (NEB, E1202S). VSG-228 was partially amplified from VSG PCR (see below ...
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bioRxiv - Biochemistry 2022Quote: ... competent cells and were purified using NEB Monarch Miniprep Kits (NEB, Canada) as per manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... coli Top10 cells and purified by using a Monarch miniprep kit (NEB). A Ty tag DNA sequence was inserted at the 3’-end of the gat1 coding sequence using a Q5 site directed mutagenesis kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... Further processing for mRNA selection was performed with Oligo-d(T)25 Magnetic Beads (NEB, E7490) and the integrity of the RNA was validated once more with Agilent 4200 TapeStation (Agilent ...
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bioRxiv - Genomics 2021Quote: ... the rest was subjected to pull-down with oligo-d(T)x25 magnetics beads (NEB-S1419S), 1ml of slurry per 1L of cell culture during 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The poly(T) adapter was ligated to the mRNA using T4 DNA ligase (New England Biolabs) in the Quick Ligase reaction buffer (New England Biolabs ...