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Citations for Illumina :
201 - 250 of 1063 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The PCR-free TruSeq DNA (Illumina, San Diego, CA) was used to prepare a library ...
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bioRxiv - Cell Biology 2023Quote: ... purified transposed DNA was PCR amplified using Nextera (Illumina) i5 common adapter and unique i7 index adapters and NEBNext High Fidelity 2x PCR Master Mix for 5 cycles with the following program ...
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bioRxiv - Neuroscience 2024Quote: Libraries were prepared using TruSeq DNA PCR-Free (Illumina) and were sequenced with 150-base-pair paired end reads on an Illumina HiSeq 4000 ...
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bioRxiv - Microbiology 2023Quote: ... Reverse and forward amplicon PCR primers recommended by Illumina were used (16s Amplicon PCR Forward Primer= TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG ...
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bioRxiv - Microbiology 2024Quote: ... with the TruSeq PCR-free library prep kit (Illumina) and the Sci clone next-generation sequencing (NGS ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were sequenced with a MiSeq (Illumina) using the reagent kit v3 (PE 2x300 ...
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bioRxiv - Microbiology 2024Quote: ... which was determined by PCR and confirmed by Illumina whole-genome sequencing for this study.
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bioRxiv - Genetics 2021Quote: ... and then resuspended in tagmentation reaction mix for 30 min (2.5uL TD1, 1X TD Buffer, Illumina Nextera DNA ...
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bioRxiv - Genetics 2019Quote: ... Cells were incubated in Tagmentation mix (Tagmentation DNA buffer Illumina 15027866; Tagmentation DNA enzyme Illumina 15027865) for 30 min at 37°C ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were then resuspended in 50 μl transposase reaction mix (1x Tagment DNA buffer (Illumina 15027866), 2.5 μl Tagment DNA enzyme I (Illumina 15027865) ...
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bioRxiv - Genomics 2021Quote: ... Pelleted nuclei were resuspended in 50 µl transposase reaction mix (1x Tagment DNA buffer (Illumina 15027866), 2.5 µl Tagment DNA enzyme I (Illumina 15027865) ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were then resuspended in 50 μl transposase reaction mix (1x Tagment DNA buffer (Illumina, 15027866), 2.5 μl Tagment DNA enzyme I (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were resuspended in 50 μL of tagmentation reaction mix as per manufacturer instructions (TDE1, Illumina) and incubated at 37°C for 30 mins with gentle agitation every 5 min ...
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bioRxiv - Immunology 2023Quote: ... 3mM MgCl2) on ice and resuspended in 10μl of transposition mix (1X TD Buffer from Illumina, Transposase from Illumina ...
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bioRxiv - Genetics 2023Quote: ... nucleus tagmentation was performed in Tn5 transposase reaction mix (Illumina Tagment DNA Enzyme and Buffer kits) under incubation at 37 °C for 30 min in a thermomixer with shaking at 1,000 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... and nuclei were resuspended in 200μl transposition mix (1x TD buffer containing X µl of Illumina Tagment DNA TDE1 Enzyme per every X nuclei) ...
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bioRxiv - Genomics 2024Quote: ... samples were incubated in cleavage mix (MiSeq Nano kit v2 reagent 4) (Illumina MS-103-1003) for 6 min at 60 °C ...
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bioRxiv - Genomics 2024Quote: ... samples were incubated in incorporation mix (MiSeq Nano kit v2 reagent 1) (Illumina MS-103-1003) for 5 minutes at 60 °C on a flat-top thermal cycler ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR products were subjected to sequencing library construction using the TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA). The libraries were loaded on a NovaSeq 6000 system (Illumina ...
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bioRxiv - Genetics 2021Quote: ... DNA was PCR-amplified with TruSeq dual indexing primers (Illumina) to generate Illumina compatible libraries ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by tailed-PCR using Nextera XT Index Primers (Illumina) (S1B Fig) ...
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bioRxiv - Genomics 2019Quote: ... After a post-capture PCR (four amplification cycles using Illumina PE PCR 1.0 and PE PCR 2.0 primers ...
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bioRxiv - Microbiology 2019Quote: ... qPCRs were performed in an Eco RT-PCR system (Illumina). Relative quantification of gene expression was determined by the 2−ΔΔCt method [25] applied with software conforming to minimum information for publication of RT-qPCR experiments (MIQE ...
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bioRxiv - Microbiology 2021Quote: ... by 7 cycles PCR (16S Metagenomic Sequencing Library Preparation, Illumina). The amplicon libraries were purified using Agencourtusing the Agencourt AMPure XP system (Beckman) ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were then quantified and sequenced using MiSeq (Illumina).
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Genomic bases of insularity and ecological divergence in barn owls (Tyto alba) of the Canary IslandsbioRxiv - Evolutionary Biology 2022Quote: ... and individually tagged 100bp TruSeq DNA PCR-free libraries (Illumina) were prepared according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... PCR amplification was performed in a 48 well plate (Illumina) on an Eco machine (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplicons were then sequenced on a NextSeq 500 (Illumina). Guide scores were calculated as median log2 fold change in the abundance between the initial and final population of that sgRNA similar to standard CRISPR screens ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary PCR was performed using Nextra XT index primers (Illumina). The secondary PCR condition was 95 °C (20 sec) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We used a Truseq DNA PCR-free LT kit (Illumina) for library preparation ...
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bioRxiv - Cell Biology 2019Quote: ... The PCR-amplified samples were sequenced on a HiSeq2500 (Illumina) at the Biomedical Sequencing Facility (CeMM/MUW).
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bioRxiv - Cell Biology 2022Quote: ... PCR amplicons were purified and sequenced on a MiSeq (Illumina). Sequencing reads were mapped and the abundance of each sgRNA was measured ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR adapters from the Illumina Nextera DNA prep kit (Illumina) were selected and added to each sample along with PCR master mix ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were then sequenced on a HiSeq400 (Illumina) with single end 100 bp reads at a minimum read depth of 5 million reads/sample.
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bioRxiv - Cell Biology 2023Quote: ... Amplified and gel purified PCR products were sequenced by Illumina HiSeq (Azenta) ...
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bioRxiv - Genomics 2023Quote: ... TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) was used as per the manufacturer’s protocol for sequencing library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR adapters from the Illumina Nextera DNA prep kit (Illumina) were selected and added to each sample along with PCR master mix ...
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bioRxiv - Microbiology 2023Quote: ... followed by PCR amplification using TruSeq-designed primers from Illumina guidelines ...
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bioRxiv - Microbiology 2023Quote: ... PCR-enriched and barcoded with unique dual indexes (Illumina, 20027213) using six PCR cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were sequenced on a HiSeq 4000 instrument (Illumina) at 50bp reads to a depth of 30M reads per sample.
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bioRxiv - Microbiology 2024Quote: ... After qRT-PCR (KAPA Library Quantification Kit Illumina® Platforms) to determine TraDIS library concentration ...
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bioRxiv - Bioengineering 2020Quote: ... Pelleted nuclei were resuspended in 25 μL Tn5 transposition mix (12.5 μL 2X Tagment DNA buffer, 1.25 μL Tn5 transposase, and 11.25 μL sterile water; Illumina) and stored in a shaking incubator at 37°C and 500 RPM for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Transposition reactions were performed in 25 µl tagmentation mix (1.25 µl Illumina Tagment DNA Enzyme (Cat# 20034197), 12.5 µl 2X Illumina Tagment DNA buffer ...
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bioRxiv - Genetics 2021Quote: ... The pellet was then resuspended in the tagmentation reaction mix (25 μL 2X TD Buffer (Illumina, 15027866), 2.5 μL TD Enzyme (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA (rRNA) was depleted from 1μg total RNA by addition of rRNA Removal Mix – Gold (Illumina) followed by incubation at 68C for 5min ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was aspirated, and pellets were resuspended in transposase reaction mix (25μL 2xTD Buffer, 2.5μL transposase (Illumina), and 22.5μL nuclease free water) ...
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bioRxiv - Genomics 2021Quote: ... 0.1% IGEPAL CA-630) and 2.5 μL DNA Tagmentation Enzyme mix (Nextera DNA Library Preparation Kit, Illumina) was added ...
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bioRxiv - Neuroscience 2023Quote: ... Pellets were resuspended in transposase reaction mix (25 μL 2x TD Buffer (Illumina Cat #FC-121-1030) 2.5 μL Tn5 Transposase (Illumina Cat #FC-121-1030 ...
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bioRxiv - Immunology 2023Quote: ... 50.000 nuclei per sample were centrifuged at 1000rcf for 5 minutes at 4°C and resuspended in transposition mix (12.5µl TD buffer, 5µl TD enzyme and 7.5µl H2O; Illumina, Catalog # 20034197). Nuclei were incubated at 37°C for 45 minutes and 800rpm ...
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bioRxiv - Neuroscience 2023Quote: ... the nuclei were extracted and resuspended with the transposase reaction mix (25 μl 2x TD buffer (Illumina); 2,5 μl Transposase (Illumina) ...