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Citations for Illumina :
251 - 300 of 1063 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... The nuclei were resuspended in transposition reaction mix (2x TD Buffer (Illumina Cat #FC-121–1030, Nextera), 2.5 µl Tn5 Transposase (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were resuspended in 50 µl transposition reaction mix with Nextera Tn5 Transposase (Illumina, FC-121-1030) and incubated for 30 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Nuclei were immediately resuspended in 25 μL of the transposition reaction mix (12.5μL 2x TD Buffer (Illumina), 1.25μL Tn5 Transposases ...
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bioRxiv - Microbiology 2020Quote: ... and TruSeq Index PCR Primer barcodes (Illumina, San Diego, CA, USA) were used to prepare and index each individual library ...
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bioRxiv - Neuroscience 2022Quote: ... on the Eco Real-Time PCR System (Illumina, San Diego, CA). Cycling parameters were as follows ...
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bioRxiv - Immunology 2021Quote: ... and indexed TruSeq Small RNA PCR primers (Illumina, San Diego, CA) as specified(Stoeckius et al. ...
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bioRxiv - Evolutionary Biology 2019Quote: ... following manufacturers instructions and TruSeq DNA PCR-Free Library (Illumina, USA) sequencing libraries were prepared after quality control ...
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bioRxiv - Immunology 2021Quote: ... Libraries were PCR-purified and sequenced on a HiSeq2500 machine (Illumina) generating 50 base pair (bp ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... and indexed TruSeq Small RNA PCR primers (Illumina, San Diego, CA) as specified(13) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina libraries were prepared with IlluminaTruSeq DNA PCR-free kits (Illumina) and sequenced as 150 bp paired-end reads on Illumina HiSeq X Ten by Macrogen Inc ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and prepared individually tagged 100bp “TruSeq DNA PCR-free” libraries (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplicons were gel purified and sequenced on a HiSeq4000 (Illumina) to obtain about 30 million reads for each samples ...
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bioRxiv - Cancer Biology 2021Quote: TruSeq DNA PCR-free Library Preparation Kit (Illumina, San Diego, CA) was performed following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... and libraries were amplified by PCR with barcoded Nextera primers (Illumina) using 2X NEBNext High-Fidelity PCR Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA libraries were tagmented and PCR-amplified (Nextera tagmentation kit, Illumina). Finally ...
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bioRxiv - Genomics 2019Quote: ... Libraries were prepared using a TruSeq DNA PCR free kit (Illumina) and sequenced in multiple runs to generate 71M read pairs ...
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bioRxiv - Cancer Biology 2022Quote: ... purified PCR products were amplified using indexed adapter primers from Illumina to generate barcoded amplicons and NEBNext Ultra II Q5 Master Mix (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... The variable sequence was PCR-amplified and sequenced (Illumina HiSeq 4000) to verify clone representation of all library component ...
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bioRxiv - Molecular Biology 2023Quote: ... PCRs were performed using index primers (NEBNext multiplex oligos for Illumina, set 1 ...
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bioRxiv - Molecular Biology 2022Quote: Cell lysate was PCR amplified with genomic primers flanked by Illumina adaptor overhang to generate approximately 300 base products ...
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bioRxiv - Molecular Biology 2023Quote: ... Library fragments were amplified using standard PCR and Nextera primers (Illumina) by adding 25 μl of 2x NEBnext master mix ...
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bioRxiv - Neuroscience 2023Quote: ... Index was added using PCR with Nextera XT Index Kit (Illumina). We used MiSeq 300 cycle ver ...
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bioRxiv - Microbiology 2023Quote: ... library tagmentation and 16-cycle PCR amplification using Nextera XT (Illumina), and 150 bp paired-end sequencing using an Illumina MiSeq or NextSeq.
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were sequenced on a HiSeq 2000 sequencing platform (Illumina). A fraction of the sgRNA plasmid library was also sequenced to determine the sgRNA distribution in the input material ...
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bioRxiv - Biophysics 2022Quote: ... and amplicons were generated by PCR for amplicon sequencing by Illumina 2×300 paired-end sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR amplicons surrounding Usp18 sgRNA cut sites were sequenced (MiSeq, Illumina) and frame-shift mutation frequency for triploid CT26 cells was estimated to be ∼65% by the following equation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 6-digit index primers (Illumina RNA PCR Index Primers RPI1-RPI28) were used instead of 10-digit index primers suggested by the LM-Seq protocol ...
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bioRxiv - Synthetic Biology 2023Quote: Sequencing of the mutagenic PCR fragments was done by NGS (Illumina). DNA samples were converted to Illumina sequencing libraries using Illumina’s Truseq NanoDNA Sample Preparation Kit (Cat ...
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bioRxiv - Genetics 2024Quote: ... PCR amplicons were gel-purified and sequenced on a NextSeq500 (Illumina). Screens were analyzed using Python (v.2.7.13) ...
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bioRxiv - Genetics 2021Quote: ... 50,000 nuclei were incubated in the transposition reaction mix (20μl nuclease-free water; 25μl 2X Tagment DNA Buffer, Illumina Cat#FC-121-1030 ...
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bioRxiv - Neuroscience 2021Quote: ... Beads were then resuspended in 25μL of tagmentation reaction mix containing 12.5μL of 2x Tagmentation DNA Buffer (Illumina #15027866) and 1μL of Tagment DNA Enzyme (Illumina #15027865) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was transposed in 50 μl of the following reaction mix: 1x tagmentation buffer (Illumina, 20034197), 1x PBS ...
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bioRxiv - Immunology 2019Quote: ... The pellet was resuspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl transposase (Illumina) and 22.5 μl nuclease-free water ...
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bioRxiv - Genetics 2021Quote: ... The nuclei were resuspended in the transposition reaction mix (2x TD Buffer (Illumina Cat #FC-121-1030, Nextera), 2.5⍰μl Tn5 Transposase (Illumina Cat #FC-121-1030 ...
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bioRxiv - Microbiology 2020Quote: Five million fungal spheroblasts or 0.5 ng naked gDNA were resuspended in the tagmentation reaction mix (12.50 μL Nextera 2xTD buffer, 2.00 μL Nextera TDE1 [all Illumina] ...
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bioRxiv - Immunology 2020Quote: ... the pellet was resuspended in 50 μL ATAC reaction mix (25uL 2X TD buffer, 2.5 μL Nextera Enzyme, 22.5 μL water, Illumina). The transposase reaction was carried out at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were then resuspended in transposase reaction mix for 30 min at 37 °C (Illumina, Fc-121-1030). The samples were purified using Qiagen MiniElute kit (QIAGEN ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting pellet was resuspended in transposase reaction mix (25μl cold lysisl 2x TD buffer, 10μl cold lysisl transposase (Illumina), 15μl cold lysisl nuclease-free H2O ...
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bioRxiv - Cancer Biology 2022Quote: ... The transposition reaction mix (25μl of 2X TD buffer, 2.5μl of Tn5 transposase (Illumina, San Diego, CA, USA), 15ul of PBS and 7.5μl of nuclease free water ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell pellet was then re-suspended in 50ml of transposition reaction mix (25ml 2x TD buffer (Illumina), 2.5ml transposase (Illumina) ...
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bioRxiv - Systems Biology 2022Quote: ... were lysed and transposed simultaneously in 25 µl of transposition mix (0.1% NP40, 0.1% Tween-20, 0.01% digitonin and 5% Tn5 enzyme (Illumina # 15027865) in Tagment DNA Buffer (FC-121–1030 ...
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bioRxiv - Molecular Biology 2022Quote: ... We used Stringtie version 2.1.7 in mix mode (Kovaka et al., 2019) to generate de novo transcriptome assemblies from Illumina RNAseq replicates and pooled Nanopore DRS data ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated nuclei were mixed with 50 µl of transposition reaction mix containing Tagment DNA Enzyme 1 (Illumina, 20034197). Samples were incubated using a thermo shaker at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina] ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was then resuspended in the transposition reaction mix (25 μL 2× TD buffer, 2.5μLTn5 Transposes (Illumina) and 22.5μL nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets gently resuspended in 50 μL of transposition reaction mix (25 μL tagment DNA buffer (Nextera, Illumina), 2.5 μL tagment DNA enzyme (Nextera ...
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bioRxiv - Genomics 2023Quote: ... DNA was tagmented by the addition of 10 µL of tagmentation mix (0.01 µL of a custom TDE1 enzyme in 9.99 µL of 2x Nexterda TD buffer, Illumina) and plates incubated at 55°C for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants were carefully removed and the pellets were re-suspended in 25 μL of transposase reaction mix (12.5 μL 2×TD buffer, 1.25 μL TN5 transposase (Illumina) and 11.25 μL nuclease-free water) ...
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bioRxiv - Synthetic Biology 2023Quote: Tagmentation Mix was prepared by mixing 1.25 μL Tagment DNA Buffer and 0.25 μL Tagment DNA Enzyme (Illumina), then added to 1 μL of plasmid DNA at a concentration of 1 ng/μL for a total reaction volume of 2.5 μL ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were resuspended in 25uL of transposition mix (12.5ul 2x TD buffer, 1.25ul transposase (Illumina, catalog nr 20034197), 8.25ul PBS ...