Labshake search
Citations for Illumina :
451 - 500 of 9336 citations for Mouse Direct PCR Kit For Genotyping since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... First strand cDNA was subjected to limited PCR amplification followed by Tn5 transposon-based fragmentation using the Nextera XT DNA Library Preparation Kit (Illumina). Samples were then PCR amplified for 12 cycles using barcoded primers such that each sample carries a specific combination of eight base Illumina P5 and P7 barcodes for subsequent pooling and sequencing ...
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bioRxiv - Immunology 2020Quote: ... the 2nd SGA env PCR products were cleaved into approximately 300 bp products by tagmentation using the Nextera DNA Library Prep Kit (Illumina). Indices from the Nextera Index Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared following the RNA-seq sample work flow using the Scriptseq Complete kit for bacteria and indexed with unique Scriptseq Index PCR primers (Illumina).
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bioRxiv - Genomics 2021Quote: ... Genomic DNA library was prepared and sequenced using a TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA).
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bioRxiv - Genomics 2021Quote: ... DNA was PCR amplified for a total of 11-13 cycles using barcoded primers (Illumina Nextera XT Index Kit v2) and purified using Ampure beads (1.4:1 beads:sample ratio) ...
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bioRxiv - Genomics 2021Quote: ... Direct tagmentation of the mRNA/cDNA hybrids and PCR amplification were performed on beads using Nextera XT DNA Library Prep Kit (Illumina); the Reference Guide was followed exactly for 20K and 10K samples ...
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bioRxiv - Genomics 2020Quote: ... The products were then amplified by 12 cycles of PCR using specific index adapters for Illumina sequencing (Nextera XT Index Kit v2, Illumina) (Supplementary Figure 1A) ...
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bioRxiv - Genetics 2020Quote: ... Two PCR-free libraries with insertion sizes of 350 bp and 550 bp were constructed for each cat using the TruSeq DNA PCR Free library preparation kit (Illumina). The Illumina HiSeq 2000 (Illumina ...
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bioRxiv - Genomics 2022Quote: Whole-genome shotgun libraries of 13 Capsicum lines were prepared with the TruSeq DNA PCR-Free Sample Prep Kit (Illumina), in accordance with the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Purified DNA was further amplified by PCR using Kapa Hifi Hotstart Ready Mix with Nextra XT Index Primers from Nextra XT Index Kit (Illumina). The PCR condition was ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were indexed using the Nextera XT Index Kit following the manufacturer’s instructions (Illumina, San Diego, CA, USA) and the concentration of the differently indexed samples determined using a KAPA Library Quantification Complete kit (Kapa Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Sequencing libraries were prepared on 1µg of DNA using the TruSeq DNA PCR-Free Library Preparation Kit (Illumina, cat#20015963), with one additional bead cleanup performed after end-repair and after adapter ligation ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 ng of PCR1 product was subjected to another round of PCR to add unique Illumina indexes (Nextera XT Index Kit, Illumina) with KAPA Hifi Hotstart Ready Mix (Roche ...
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bioRxiv - Immunology 2023Quote: ... Samples were then indexed by ligating index barcode to Illumina adapters onto the PCR amplicon using the Nextera XT Index Kit v2 Set A (Illumina). Indexed amplicon DNA concentrations in each sample were quantified ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared and indexed by adapter ligation and PCR (8 cycles) using the TruSeq Nano DNA Library Prep Kit (Illumina) and TruSeq DNA Single Indexes Set A or B (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... an individually tagged library was prepared from 125 ng of input material using the TruSeq DNA PCR-Free kit (Illumina). Since pools of amplicons do not need to be fragmented ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... we utilized an Illumina DNA Prep kit with Nextera DNA CD Indexes to create an NGS library from the purified PCR products (Illumina). The libraries were then pooled and transferred onto a MiniSeq sequencer (Illumina).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... We then performed a second PCR step in which unique dual-index combinations were appended to the amplicons using the Nextera XT index kit (Illumina). Second-step PCRs were performed in a 25 μL ...
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bioRxiv - Microbiology 2023Quote: ... Indices were added in a second PCR over 8 cycles with unique primer combinations using the Nextera XT Index Kit V2 (Illumina). The samples were pooled and cleaned using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Zoology 2023Quote: ... DNA fragments were used to generate genomic libraries using the TruSeq DNA PCR-free library kit (Illumina, San Diego, CA), which were quality checked on an Agilent 4200 Tapestation and quantitated using a Lightcycler qPCR assay (Roche Life Science ...
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bioRxiv - Plant Biology 2023Quote: ... A paired-end library with insert sizes ranging from 450 to 550 bp was constructed using the TruSeq DNA PCR-Free Library Prep kit (Illumina) and was size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were then performed by PCR with a library preparation kit (Vazyme, TD501) and sequencing was done using the Novaseq system (Illumina) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... 200 pg of purified WTA products from each pool were subjected for library construction with custom designed New-P5-SMART-PCR primer using the Nextera XT DNA library preparation kit (Illumina) following the detailed protocol provided by the manufacture ...
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bioRxiv - Microbiology 2024Quote: ... The following index PCR for both gene libraries was conducted with the Nextera XT index kit v2 (Illumina Inc., USA). The PCR product was purified using the LabopassTM DNA purification kit (Cosmogenetech Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed for 40 cycles (15 s at 95°C and 45 s at 60°C) using a Thunderbird SYBR Green Polymerase Kit (TOYOBO) and Eco Real-Time PCR System (Illumina).
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bioRxiv - Molecular Biology 2024Quote: ... Dual indices and Illumina sequencing adapters were attached to the amplicons with index PCR using Nextera XT Index Kit v2 (Illumina). The libraries were combined with the PhiX Control v3 (Illumina) ...
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bioRxiv - Cell Biology 2024Quote: ... At least 200□pg of amplified cDNA were used for tagmentation reaction and subsequent PCR amplification using the Nextera XT kit (Illumina) to construct sequencing libraries ...
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bioRxiv - Evolutionary Biology 2024Quote: ... which were used to construct a genomic library using the TruSeq DNA PCR-free library kit (Illumina, San Diego, CA). Library quality was assessed with an Agilent 4200 Tapestation and Lightcycler quantitative PCR (Roche Life Science ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... The generated cDNA was 3′ end- adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Evolutionary Biology 2024Quote: Illumina sequencing libraries for three amplified DNA samples were prepared using Nextera TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina). Subsequently ...
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bioRxiv - Genomics 2024Quote: ... The sequencing library was prepared with 1 μg of DNA using the TruSeq PCR-free DNA sample preparation kit (cat# 20015962, Illumina), according to the TruSeq DNA PCR-free Reference Guide ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and paired-end libraries were constructed according to the Illumina TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina). The four paired-end libraries were quantified by qPCR using the KAPA Library Quantification Kit (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Between 350 and 500ng of tumour DNA were used to prepare DNA sequencing libraries using the TruSeq DNA PCR Free 350bp kit (Illumina). The samples were then sequenced on the Illumina NovaSeq6000 sequencing machine using S4 flow cells to generate 2 x 151 paired-end reads ...
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bioRxiv - Genomics 2019Quote: ... followed by the removal of ribosomal RNA using a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Illumina). Strand-specific sequencing libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Genomics 2019Quote: ... RNA sequencing libraries were made using the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat kit (Illumina) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2019Quote: ... For whole genome sequencing of mouse RNA a library was generated with Truseq Small RNA Library Preparation kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA- Seq library was prepared by the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Illumina, RZG1224) and TruSeq Stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Ribosomal reduction libraries were prepared with the Ribo-Zero Gold rRNA removal kit (human/mouse/rat) MRZG12324 (Illumina, USA) and sequenced at the Yale University Center of Genome Analysis (YCGA ...
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bioRxiv - Molecular Biology 2021Quote: ... Depletion of ribosomal RNA was carried out solely by use of the RiboZero Gold Human/Mouse/Rat kit (Illumina). Adapter sequences were based on the TruSeq small RNA sequences (Illumina) ...
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bioRxiv - Genomics 2022Quote: ... RNA-seq libraries were prepared from 600 ng of total RNA (RNA integrity number (RIN) >8.0) with TruSeq Stranded Total RNA Human/Mouse/Rat kit (Illumina). For polysome immunoprecipitation experiments ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... RNA-seq libraries were prepared using the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat kit (Illumina) according to manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... with the exception that a two-step PCR was used for all reactions (first PCR using untailed primers, second PCR using Illumina adapter-tailed primers), even if a one-step PCR was used in the original protocol ...
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bioRxiv - Genomics 2020Quote: ... The purified PCR reactions were pooled together and then sent for paired-end Illumina MiSeq sequencing (Illumina 2 × 250 v2 kit) at the Edinburgh Genomics Facility (University of Edinburgh ...
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bioRxiv - Cancer Biology 2021Quote: ... indexed libraries for human whole genome sequencing were constructed from 1.0 µg gDNA using the TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Illumina barcodes and adapters were attached to pooled and purified products in a second PCR (8 cycles) with the Nextera XT Index Kit A and D (Illumina Inc.). Libraries were purified with Agencourt AMPure XP kit (Beckman coulter ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Doyle and Doyle 1987) and subjected to PCR (for Sanger sequencing) or rolling-circle amplification with the GE TempliPhi kit (for Illumina sequencing) per the manufacturer's instructions ...
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bioRxiv - Bioengineering 2019Quote: ... sequencing adaptors and unique dual barcodes combinations were attached to each library PCR amplicons using the Nextera XT Index Kit primers (index 1 N7XX and index 2 S5XX, Illumina, CA), and the amplicon size and concentration were confirmed using a Bioanalyzer (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... Sheared DNA was used to construct the library using the Illumina TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina, 20015962). After adapter ligation ...
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bioRxiv - Plant Biology 2020Quote: ... The sequencing step of PCR fragments was done on an Illumina Miseq personal sequencer using the MiSeq Reagent Kit v3 (Illumina®) followed by quality control processes for libraries using the PippinHT system from SAGE Sciences for libraries purification ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of gDNA per sample was analyzed using the TruSeq® DNA PCR-Free Low-Throughput Library Prep Kit (Illumina) according to the manufacturer’s directions ...