Labshake search
Citations for Illumina :
3151 - 3200 of 3798 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Alignment of RNA-seq reads was performed with the STAR aligner and differential gene expression was calculated with DESeq2 using the RNA Express software suite (Illumina). A p value < 0.05 was used as threshold for significant changes in gene expression ...
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bioRxiv - Microbiology 2022Quote: ... plate-based RNA sample prep was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Illumina Ribo-Zero rRNA Removal Kit (Bacteria) and the TruSeq Stranded Total RNA HT sample prep kit following the protocol outlined by Illumina in their user guide ...
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bioRxiv - Cancer Biology 2022Quote: ... The samples were sequenced at the Genomic and RNA Profiling Core at Baylor College of Medicine on Nextseq 500 or NovaSeq 6000 sequencing machines from Illumina.
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bioRxiv - Biochemistry 2022Quote: ... 50 ng of DNase I treated total RNA and three-stage purified mRNA from the two biological replicates were fragmented using the TruSeq RNA Library Prep Kit v2 fragmentation buffer (Illumina). First-strand cDNA synthesis was performed using the random hexamer primer ...
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bioRxiv - Developmental Biology 2022Quote: ... poly(A)-selected RNA-seq libraries were prepared by Novogene Co. using the NEBNext UltraII RNA library prep kit for Illumina (cat. #E7775) and sequenced on a NovaSeq6000 (Illumina) to produce paired-end 150-bp reads (average 40.4 million reads per sample) ...
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The RNA-Binding Protein SAM68 regulates cardiomyocyte differentiation by enhancing Gata4 translationbioRxiv - Molecular Biology 2022Quote: ... rRNA depletion and library preparation was done with the TruSeq stranded total RNA Library prer Human/Mouse/Rat (20020596, Illumina). The sequencing was performed using 2×125bp paired-ends reads on a HiSeq 2500 sequencer with HiSeq v4 chemistry ...
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bioRxiv - Immunology 2022Quote: ... 1000ng RNA from each sample was used as a template for preparing Illumina compatible libraries using the TruSeq RNA Library Prep Kit v2 (Illumina). Library sizes were checked using D5000 high sensitivity tape on the TapesSation 2200 (Agilent) ...
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bioRxiv - Molecular Biology 2022Quote: ... MEFs and NPCs were used to prepare NGS libraries based on the NEB Next Ultra II directional RNA library preparation kit from Illumina. As default ...
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bioRxiv - Microbiology 2022Quote: ... 1 ug of total RNA from each tissue was enriched for polyadenylated RNA and prepared for next-generation sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... prior to their RNA sequencing (paired-end 150 bp, with 12 Gb output) on an Illumina NovaSeq 6000 (Illumina, USA).
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bioRxiv - Neuroscience 2022Quote: ... prior to their RNA sequencing (paired-end 150 bp, with 12 Gb output) on an Illumina NovaSeq 6000 (Illumina, USA). Program parameter settings for library build ...
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bioRxiv - Microbiology 2022Quote: ... RNAseq libraries were prepared from total RNA using the Nextera DNA Flex library preparation kit (Illumina, Inc., San Diego, CA) according to the manufacturer’s instructions where the first-strand cDNA was synthesized using SuperScript II Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: ... RNA sequencing was next conducted after establishing sequencing libraries using an IlluminaXten sequencing system (Illumina Inc., San Diego, CA, USA) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... RNA library was constructed with 10x-Genomics Single Cell 3’ RNA sequencing kit v3 as previously described(18) sequenced using a NextSeq500 (Illumina). Cell clusters were characterized using an automated annotation tool(19 ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were constructed using NEB polyA RNA ultra II and subsequently subjected to 150 cycles of sequencing on NovaSeq-6000 (Illumina). Adapters were trimmed using Cutadapt (v2.3) ...
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bioRxiv - Neuroscience 2021Quote: ... ~600 pg of purified cDNA was used to produce a RNA-seq library using Nextera XT DNA Library Preparation kit (FC-131-1024, Illumina), following the manufacturer instructions.
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bioRxiv - Cell Biology 2021Quote: ... library construction (250-300 bp insert cDNA library) and RNA sequencing (Illumina HiSeq X Ten, paired-end, 10 M reads). Quality controls were included for the total RNA samples and during the procedures for RNA-sequencing.
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bioRxiv - Cancer Biology 2020Quote: ... then 60 ng was used for library preparation using KAPA Stranded RNA-Seq Library Preparation Kit (Illumina, KR0934 – v 1.13). The samples were barcoded and pooled in sets of eight per run to generate paired 150-bp reads with NextSeq 550 Sequencing System (Illumina) ...
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bioRxiv - Plant Biology 2021Quote: ... Samples with a RIN value of > 7 were used for library preparation (Illumina TruSeq Stranded Total RNA kit, Illumina, USA) and sequenced on an Illumina HiSeq 4000 to obtain 150bp paired-end reads ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA samples with RIN>8.0 were shipped to Beijin Genome Institute for a library preparation and sequencing (Illumina HiSeq 2500). Integrative Genomics Viewer (2.4.14 Broad Institute ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: The RNA-seq libraries were made with NEBNext Ultra II Directional (PolyA) kit and sequenced with NovaSeq SP 2×150bp (Illumina) at Biomedicum Functional Genomics Unit (FuGU) ...
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bioRxiv - Neuroscience 2020Quote: ... we used one batch of 20 ng of total photoreceptor (n = 8) RNA and 30 ng for the other three batches (n = 24) according to the manufacturer’s protocol (Illumina Platforms). For generating libraries from RPE samples we used 20 ng of RNA ...
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bioRxiv - Microbiology 2021Quote: ... cDNA library was prepared by using NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolab, Cat# E7530) and whole genome sequencing was performed by Miseq (Illumina).
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bioRxiv - Microbiology 2021Quote: ... All RNA-seq and RIP-seq experiments were performed thrice with RNA samples obtained from independently prepared mycelia using the NextSeq 500 system (Illumina) and mapped to the A ...
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bioRxiv - Cancer Biology 2021Quote: Barcoded RNA libraries were prepared starting with 1ug for MCF-10A and 500ng for HCC1806 cell lines of total RNA using the TrueSeq stranded mRNA kit with polyA selection (Illumina), and quantified using a Bioanalyzer DNA 1000 chip (Agilent) ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were sequenced by paired-end sequencing with a 75 (patient samples) or 150 (nascent RNA-seq) cycle high-output Nextseq 500 kit (Illumina).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sequencing library was prepared using the Illumina TruSeq RNA Sample Prep Kit (FC-122-1001; Illumina, San Diego, CA) with 1 ug of total RNA ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA-seq libraries were sequenced for 75 cycles in single-end mode on NextSeq 500 platform (Illumina, FC-404-2005).
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bioRxiv - Microbiology 2022Quote: ... RNA was purified using QIAGEN RNeasy kit and ribosomal RNA was depleted using the Ribo-Zero rRNA removal magnetic kit (Illumina). The cDNA library was generated using the NEBNext Ultra II RNA Library Prep Kit and each replicate was barcoded in the DNA library using the NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Microbiology 2022Quote: For RNA-seq analysis an Agilent Bioanalyzer was used to verify RNA quality before depleting rRNA and ligating adapters and indexes using a Stranded Total RNA with RiboZero Plus library preparation kit (Illumina). Samples were sequenced as single-end 75 reads on an Illumina NextSeq 500 sequencer at the Tufts University Genomics Core Facility ...
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bioRxiv - Genetics 2022Quote: ... Extracted RNA with RNA integrity number (RIN) ≥7 was used as input for the TruSeq Stranded mRNA HT Sample Prep Kit (Illumina) according to the manufacturer ‘s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: Listed are the availability of RNA-seq data (indicated by “+”) for the Illumina and various Oxford Nanopore platforms and characteristic gene fusions identified by Illumina-based sequencing.
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bioRxiv - Cell Biology 2022Quote: ... RNA-seq libraries were prepared using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina). We generated biological triplicate data for 1T47 ...
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bioRxiv - Cell Biology 2020Quote: ... data shown are from pseudo-counts-RLE obtained after RNAseq analysis of triplicate samples from RNA banks made with the TruSeq Stranded mRNA sample preparation kit (Illumina) and sequencing with a HiSeq2500 Illumina sequencer (MGX Montpellier GenomiX ...
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bioRxiv - Plant Biology 2020Quote: ... Next-generation sequencing libraries were prepared using an Ovation SoLo RNA-Seq System following the instructions of the manufacturer (Nugene) and sequenced on a NextSeq500 system (Illumina) as 70 bp single-end reads with an average quality score (Q30 ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng alkylated RNA was used and prepared with a commercially available kit (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and PCR Add-on Kit for Illumina ...
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bioRxiv - Molecular Biology 2020Quote: PolyA+ RNA seq libraries were prepared using the SMART-Seq v4 UltraLow Input RNA kit (Clonetch) followed by the Nextera XT DNA library Prep kit (Illumina) according to the manufacturer recommendations from 3 biological replicates for each condition (P7 wild-type (WT) ...
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bioRxiv - Physiology 2020Quote: ... High throughput RNA-Sequencing was performed at the NIDDK Genomic Core Facility (NIH, Bethesda, MD) using the HiSeq 2500 instrument (Illumina). Raw sequences were passed through quality control and were mapped to mouse (mm9 ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were subsequently sequenced using 100bp single-end high-throughput mRNA sequencing (RNA-seq) on an Illumina Hiseq 2500 (Illumina) at Norwegian Sequencing Centre (Oslo ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq for the mouse heart tissues were performed using next generation high throughput sequencing method (Illumina HiSeq 2000, Illumina). Total RNAs were isolated ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq for the mouse heart tissues were performed using next generation high throughput sequencing method (Illumina HiSeq 2000, Illumina). Total RNAs were isolated ...
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bioRxiv - Molecular Biology 2020Quote: ... This rRNA-depleted RNA was used as input to generate paired-end libraries using the TruSeq Stranded Library Kit (Illumina) which were then pooled and sequenced on two High Output NextSeq 550 flow cells with a 75 base paired-end protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was purified from total RNA samples (100-500 ng) with oligo(dT) magnetic beads and sequencing libraries recommended by Illumina. Purified libraries were qualified on an Agilent Technologies 2200 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples with RNA integrity number (RIN) > 9 were used for sequencing library construction with the TruSeq Stranded mRNA sample preparation protocol (Illumina).
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bioRxiv - Microbiology 2020Quote: ... The transcripts were fragmented and used as templates to generate strand-specific cDNA libraries by TruSeq Stranded Total RNA LT Sample Prep kit (Illumina). Each sample was sequenced using 100-bp paired-end reads on an Illumina HiSeq 2500 instrument ...
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bioRxiv - Microbiology 2021Quote: ... and 12 of the highest scoring samples (eight LRS and four controls) were sent to Otago Genomics Facility for sequencing using the Truseq Total RNA Library Preparation Protocol (Illumina). Host ribosomal RNA (rRNA ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-seq libraries were prepared from total RNA after rRNA depletion with the Ribo-Zero rRNA Removal Kit (Plant Leaf) (Illumina) using the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Two hundred ng of total RNA were used to generate libraries using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina). Single-end reads of 100bp for each sample were produced with Illumina’s HiSeq2500v4 instrument ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were subjected to library RT and amplification to tag the RNA molecules with specific and unique sample indexes (Illumina), followed by a final beads cleanup (1:0.8 ...
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bioRxiv - Genomics 2021Quote: ... RNA libraries were prepared for sequencing by the Cancer Genomics Shared Resource (Wake Forest School of Medicine, Winston-Salem, NC) using the TruSeq-stranded total RNA kit (Illumina), which includes a ribosomal depletion step ...