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Citations for Illumina :
101 - 150 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... A one-step gentle membrane permeabilization and DNA transposition was performed by adding 50ul transposition mixture (25 uL TD buffer 2X, 2,5uL of transposase TDE1 (Illumina, San Diego, California), 0,5 uL digitonin 0,1% (Promega ...
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bioRxiv - Immunology 2020Quote: ... The cell pellets were resuspended in 50 μl transposition mix (25 μl 2X TD buffer, 2.5 μl transposase (Illumina, FC-121-1030), 16.5 μl PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell pellet was resuspended in 50 μl transposition mix (25 μl 2X TD buffer, 2.5 μl transposase (Illumina, FC-121-1030), 16.5 μl PBS ...
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bioRxiv - Plant Biology 2023Quote: ... The nuclei pellet was then immediately resuspended in 25 μ L of 2x tagmentation buffer containing 2 μ L of TDE1 (Illumina). The reaction was placed at 37C for 30 minutes with gentle mixing three times ...
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bioRxiv - Evolutionary Biology 2024Quote: ... resuspended in 25 μL of 2x TD buffer and 2.5 μl of Tn5 transposase from the Nextera DNA Library Prep kit (Illumina, San Diego, CA), and incubated at 37°C for 30 minutes ...
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Genetic underpinnings of predicted changes in cardiovascular function using self supervised learningbioRxiv - Systems Biology 2024Quote: ... 96 amplified cDNA samples were pooled and 12ng from each pool was tagmented by adding 1uL of TDE1 and 2x Tagment DNA buffer (Illumina, Ref# 20034197) in 60uL followed by PCR amplification with Kapa HiFi HotStart ReadyMix (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... TN5 transposase was added to the buffer solution (Nextera DNA-library preparation kit, Illumina, Cat# FC-121-1030 ...
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bioRxiv - Genomics 2021Quote: ... Libraries were then pooled to a final concentration of 1.75 nM and sample was sequenced in paired-end 2x 100 bp mode on a NovaSeq 6000 (Illumina, San Diego, sUS) using a S1 chip ...
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bioRxiv - Synthetic Biology 2022Quote: ... and sequenced 2x×150bp by MiSeq (Illumina). A quarter of the MiSeq run (1.2Gb ...
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bioRxiv - Genomics 2020Quote: ... eluted with Suspension Buffer (Nextera XT DNA Sample Preparation Index Kit, Illumina), and transferred to a new tube ...
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bioRxiv - Microbiology 2023Quote: ... After pooling the samples together in equimolar concentrations in resuspension buffer (Illumina), the MiniSeq denaturation protocol A (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... and all samples were eluted in 22 µL of Resuspension Buffer (Illumina). As with the Amplification sets ...
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bioRxiv - Neuroscience 2023Quote: ... and all samples were eluted in 22 μl of Resuspension Buffer (Illumina). All samples were quantitated using PicoGreen using Molecular Bynamics M2 SpectraMax instrument ...
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bioRxiv - Genomics 2020Quote: ... The resulting nuclei were resuspended in 50µl of transposition reaction buffer (25µl of 2x TD buffer from the Nextera kit, Illumina; 2.5µl of Tn5 transposase enzyme from the Nextera kit, Illumina; 22.5µl of nuclease free water), and incubated at 37°C for 90 min for chromatin tagmentation ...
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bioRxiv - Genetics 2020Quote: ... one unit of input was diluted to 30 μl and mixed with 50 μl of 2x TD buffer and 20 μl of TDE (Illumina 20034197, Lot 20436911), and incubated at 55 °C for 10 min (Note ...
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bioRxiv - Genetics 2021Quote: ... The nuclei were resuspended on ice in the transposition reaction mix (2x TD Buffer, 2.5μL Tn5 Transposes and Nuclease Free H2O) (Illumina Cat #FC-121-1030, Nextera) on ice and the transposition reaction was incubated at 37°C for 45 min ...
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bioRxiv - Genomics 2023Quote: ... The pelleted nuclei were gently resuspended with 50 µl of transposition reaction containing 25 µL 2X Tagmentation buffer (Illumina Cat FC-121-1030), 5 µL Tn5 transposase (Illumina Cat FC-121-1030 ...
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bioRxiv - Immunology 2024Quote: ... Nuclei were resuspended in the Nextera transposition reaction mix (25 μL 2x TD Buffer, 2.5 μL Nextera Tn5 transposase (Illumina Cat #FC-121-1030), and 22.5 μL nuclease free H2O ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were sent to Admera Health for library construction (SMARTseq V4 + Nextera XT) and sequencing (Illumina 2x 150bp reads, 40M read depth).
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... of amplified cDNA was added to 10 μL Tagmentation Buffer (Buffer TD from the Nextera XT DNA Sample Prep Kit, Illumina), 1 μL nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted by centrifugation at 2500g for 10 min at 4°C and resuspended in 25 µL of 2x TD Buffer (Illumina Cat #FC-121-1030), containing 8 µL of Tn5 Transposes (Illumina Cat #FC-121-1030 ...
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bioRxiv - Microbiology 2021Quote: ... Ethanol precipitated samples were resuspended in FPF sample buffer from the TruSeq stranded mRNA-Seq kit reagents (Illumina, San Diego, CA). mRNA abundance was quantified using a Quant-iT RNA Assay kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... to generate 2x 150bp paired-end reads or 2x 300 bp reads on an Illumina MiSeq (Illumina, San Diego, CA, USA). De novo assembly was also performed identical to the method as described in Coolen et al ...
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bioRxiv - Genetics 2023Quote: ... all RNA samples were diluted to 93.3 ng/uL in ELB buffer (Illumina #20020599). Next ...
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bioRxiv - Genetics 2024Quote: ... Samples were preamplified using High-Fidelity 2X PCR Master Mix (New England Bio-labs, #M0541L) and adapters (Illumina Nextera XT Index kit #FC-131-1001). The number of additional cycles was determined by quantitative PCR ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing were performed on the Illumina HiSeq 4000 paired end at 2x 100 bp (2x at least 40 million reads) using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Biochemistry 2020Quote: ... 1X TD buffer and 0.5μL TDE1 enzyme from the Nextera DNA Sample Prep Kit (Illumina). Tagmentation reactions were cleaned up using a Nucleospin column and eluted in 15μL elution buffer ...
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bioRxiv - Genomics 2021Quote: ... Transposition reaction mix containing transposition buffer and 2.5 ul of Tn5 enzyme per sample (Illumina) was added to the pellet and incubated for 30 min at 37 C ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing of 2x 100 bp was done on NovaSeq6000 (Illumina) with a sequencing depth of 50 Mio reads per sample.
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bioRxiv - Cancer Biology 2022Quote: ... followed by paired-end (2x 150bp) RNA-seq by Illumina HiSeq 2500 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA libraries were sequenced using a HiSeq 2X platform (Illumina) to generate 150bp pair-end reads ...
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bioRxiv - Microbiology 2020Quote: ... Library normalization was conducted using Illumina TruSeq DNA PCR-free sample preparation kit buffer (Illumina, USA). To prepare for cluster generation and sequencing ...
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bioRxiv - Microbiology 2024Quote: ... About 28ng of gDNA of each sample was tagmented using TD buffer and TDE1 enzyme (Illumina) and incubated in a thermocycler at 55°C for 5min prior to melting at 95°C for 0.5min ...
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bioRxiv - Genomics 2024Quote: ... The sample was then washed for 10 minutes with PR2 buffer (supplied with Illumina MiSeq kit) three times at room temperature (RT ...
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bioRxiv - Cancer Biology 2022Quote: ... The nuclei were centrifuged at 500g for 5 minutes at 4°C and subsequently resuspended on ice in 50μl transposase reaction buffer containing 2.5μl of Tn5 transposase and 25μl of 2xTD buffer (Nextera DNA Sample preparation kit from Illumina). After incubation at 37°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... resuspended in 50 μl 1xTD buffer containing 2.5 μl TDE1 transposase (Illumina Nextera DNA Sample Preparation Kit), and incubated for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Samples were processed for bulk RNA-sequencing by Q2 solutions using the TruSeq Stranded mRNA kit (Illumina: RS-122-2103) for library preparation ...
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bioRxiv - Microbiology 2021Quote: ... 5 µL of the sample was then further diluted and denatured with 5 µL 0.1N NaOH and 490 µL HT1 buffer (Illumina). Samples were sequenced on a HiSeq2500 HighOutput (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells were re-suspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl Tn5 transposase [in the Nextera DNA Sample Preparation Kit, Illumina, Cat ...
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bioRxiv - Genomics 2023Quote: ... about 40 ng purified pre-amplified Hi-C sample was fragmented in two 50 μl tagmentation mix (1x TD buffer and 0.5 μl TDE1 (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting crude nuclear pellet was then resuspended in 50 ul of tagmentation solution (1x TD buffer (Illumina, San Diego, CA 20034197), 33% PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... obtained from TBXT shRNA sOPTiKD hESC in the presence and absence of Tetracycline (three biological replicates) were harvested and samples were prepared using the Illumina Tagment DNA Enzyme and Buffer Small Kit (Illumina), 1% Digitonin (Promega ...
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bioRxiv - Genomics 2021Quote: ... Following immunoprecipitation with Dynabeads Protein G beads (invitrogen) in PCR tubes, samples were subject to Tagmentation (5 µl Tagmentation Buffer, 1 µl Tagmentation DNA Enzyme (Illumina), 19 µl Nuclease free water ...
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bioRxiv - Neuroscience 2020Quote: Tagmentation was performed per pool by adding 3 μL double-stranded cDNA sample to 5.5 μL Nextera TD buffer (Illumina, 15027866), 2.5 μL NF H2O and 1.0 μL TDE1 Enzyme (Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The nuclei pellet was resuspended in 50 mL transposition reaction mixture which includes 25 μL Tagment DNA buffer (Nextera DNA sample preparation kit; Illumina), 2.5 μL Tagment DNA enzyme (Nextera DNA sample preparation kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Each library was sequenced to 2x human genome coverage on Novaseq sequencer (Illumina, CA).
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bioRxiv - Immunology 2021Quote: ... Cells were then lysed and fragmented in a single reaction (12.5 µl 2X Illumina TDE buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina sequencing libraries were generated from the amplified cDNA samples using the Illumina Tagment DNA Enzyme and Buffer kit (Illumina, CA). These libraries were examined by Qubit and Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: Paired-end (2x 150bp) sequencing was performed using a Novaseq 6000 sequencer (Illumina Inc., USA). The FASTQ files were evaluated for quality using FASTQC (v0.11 ...
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bioRxiv - Immunology 2024Quote: The results of TCR repertoire sequencing (TRB or TRA chains, 2x 150bp paired Illumina reads) were processed by in-house developed software using both reads to build a consensus sequence covering the complete CDR3 region and removing inconsistent non-overlapping read-pairs ...