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Citations for Illumina :
351 - 400 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 0.75 M sucrose) and R1804M Ready-Lyse lysozyme solution (Illumina, USA). After 15 minutes of incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... was performed by incubating beads with tagmentation buffer (12.5 µl 2 x TD buffer, 11.5 µl nuclease free water, 1µl Tn5 enzyme (Illumina #FC-131-1024)) at 37°C for 10min ...
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bioRxiv - Genomics 2021Quote: ... The 20 ul preamp was then mixed with 25 ul TD buffer and 5 ul TDE1 buffer (Nextera kit, FC-131-1096, Illumina) and incubated for 5 min at 55 C and held at 10 C ...
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bioRxiv - Genomics 2023Quote: ... Lysate was centrifuged for 10 min at 500g at 4°C and then resuspended in transposition buffer (25 ul TD buffer and 2.5 ul TD enzyme (Illumina, 20034197), 16.5 ul PBS ...
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bioRxiv - Neuroscience 2024Quote: ... were pelleted at 500 g for 10 minutes at 4°C in a fixed angle centrifuge and resuspended in 50 μL transposition buffer (prepared from Illumina kit components TD buffer and 100 nM transposase (Illumina), as well as 0.01% digitonin ...
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bioRxiv - Neuroscience 2024Quote: ... were pelleted at 500 g for 10 minutes at 4°C in a fixed angle centrifuge and resuspended in 50 μL transposition buffer (prepared from Illumina kit components TD buffer and 100 nM transposase [Illumina] ...
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bioRxiv - Immunology 2021Quote: ... High-quality RNA was processed using an Illumina TruSeq RNA sample prep kit v2 or TruSeq Small RNA Sample Prep kit following the manufacturer’s instructions (Illumina). Clusters were generated using TruSeq Single-Read Cluster Gen ...
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bioRxiv - Microbiology 2020Quote: ... Each sample was then made compatible for deep sequencing using the Nextera XT DNA sample preparation kit (Illumina, USA). Specifically ...
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bioRxiv - Plant Biology 2021Quote: ... Pre-Release Protocol Rev.A with TruSeq RNA Sample Prep Kit and TruSeq Small RNA Sample Prep Kit (Illumina Inc.). The library was reverse transcribed ...
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bioRxiv - Pathology 2022Quote: ... Finally, the libraries of multiplexes (control, 5 samples; schizophrenia, 10 samples) were pooled and analyzed using Illumina HiSeq1500 (Illumina).
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bioRxiv - Genomics 2020Quote: Bacterial mRNA-enriched samples were subjected to library construction by TruSeq Stranded mRNA sample preparation kit (Illumina, CA, USA). The obtained cDNA libraries were subjected to RNA-seq on a high-throughput MiSeq Illumina apparatus ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA library of each sample was prepared using Tru-Seq RNA Sample Prep Kits (Illumina, San Diego, CA) for 100 bp paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... gDNA samples were converted to Illumina sequencing libraries using Illumina’s DNA Prep Sample Preparation Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... RNA-seq sequencing libraries were prepared from the purified RNA samples using IlluminaTrueSeq mRNA sample Prep kit (Illumina, U.S.) and MICROBExpress kit (Invitrogen™ ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of RNA with integrity number (RIN) > 9.5 from each sample was used for library construction with TruSeq RNA Sample Prep Kit v2 (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... a custom second-strand synthesis buffer (Illumina, USA) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... 1x TD buffer (both Illumina FC-121-1030), 1x PBS ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was homogenised in Mammalian Polysome Buffer (Illumina) supplemented with DNAse I (10 U/mL) ...
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bioRxiv - Genomics 2023Quote: ... 4 washes with PR2 buffer (PR2, Illumina MiSeq), and 6 minute incubation at 60°C with shaking followed by 4 washes was performed a total of 3 times ...
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A new vulnerability to BET inhibition due to enhanced autophagy in BRCA2 deficient pancreatic cancerbioRxiv - Cancer Biology 2023Quote: ... after which a second-strand synthesis buffer (Illumina), dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... 1x TD buffer and 100 nM transposase (Illumina Tagment DNA Enzyme and Buffer Small Kit) ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 1X Tagment DNA buffer (Illumina) with 2.5 μl Tagment DNA Enzyme (Illumina ...
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bioRxiv - Genomics 2024Quote: ... Cells were washed with MiSeq buffer PR2 (Illumina), and perturbation barcodes were analyzed by sequencing-by-synthesis using reagents from used Illumina NextSeq 2000 P2 cassettes ...
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bioRxiv - Systems Biology 2022Quote: ... in Tagment DNA Buffer (FC-121–1030; Illumina). The transposition reaction was incubated at 37 °C for 30 mins in a thermomixer with shaking at 1,000 rpm ...
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bioRxiv - Genomics 2024Quote: ... Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina) was used for this step ...
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bioRxiv - Genetics 2021Quote: ... The permeabilized myonuclei were tagmented with tagmentation mixture optimized for use on a myofiber (20 µL Tagment DNA Buffer (TD Buffer) (Illumina, 20034197), 13.3 µL PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Spheroplasts were washed twice with 500 μL spheroplasting buffer then resuspended in 50 μL 1x TD buffer with TDE (Illumina 20034197). Tagmentation was performed for 30 minutes at 37°Con a rotator and DNA was purified using the DNA Clean and Concentrator 5 kit (Zymo Research D4004) ...
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bioRxiv - Developmental Biology 2024Quote: ... The Tagment DNA Buffer and TDE1 enzyme are both contained in the Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, 20034197). The transposed chromatin was isolated using the Minelute PCR Cleanup kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The qualified RNA samples were then used for library construction following the specifications of the TruSeq RNA Sample Preparation v2 Guide (Illumina), and RNA sequencing was conducted on an Illumina Hiseq 2500 at Shanghai Personal Biotechnology Co. ...
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bioRxiv - Developmental Biology 2021Quote: ... and sequenced in 26-91bp paired-end reads run for V2 samples (Dnmt3LWT and Dnmt3LKO) and 28-91bp paired-end reads run for V3 samples (all other samples) on NovaSeq 6000 (Illumina).
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bioRxiv - Plant Biology 2022Quote: ... mRNA enriched samples were converted to libraries using True-Seq stranded RNAseq Sample Prep kit (Illumina, San Diego, CA, USA), multiplexed ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we conducted RNA-seq library preparation with 1000 ng of total RNA per tissue sample as input and following the protocol of the Standard TruSeq Stranded mRNA Sample Prep Kit (Illumina) with indexing adapters ...
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bioRxiv - Genomics 2020Quote: ... DNA samples were processed using the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA) on the Hamilton Microlab Star (Hamilton Robotics ...
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bioRxiv - Evolutionary Biology 2020Quote: The strand-specific RNA-seq libraries for all the RNA samples were prepared using the TruSeq Stranded mRNA LT Sample Prep kit (RS-122-2101, Illumina) with 1 µg total RNA as input ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was subjected to mRNA poly-A enrichment before libraries were generated with TruSeq Stranded mRNA sample preparation kit (Illumina). The libraries were sequenced paired ended (2×75bp ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 10ng of total RNA per sample was used for library construction with the Ultra-Low-RNA-seq RNA Sample Prep Kit (Illumina) and sequenced using the Illumina HiSeq 4000 instrument according to the manufacturer’s instructions for 100bp paired end read runs ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 85ng of total RNA per sample was used for library construction with the Ultra-Low-RNA-seq RNA Sample Prep Kit (Illumina) and sequenced using the Illumina HiSeq 2500 instrument according to the manufacturer’s instructions for 100bp paired end read runs ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were either paired-end sequenced using 2×75 bp read length (Replica R2 samples) or single-end sequenced using 50 bp read length (Replica 1 samples) on an NextSeq 500 system (Illumina). RNA-seq analysis was performed according to (76) ...
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bioRxiv - Neuroscience 2022Quote: ... approximately 10 ng of total RNA per sample was used for library construction with the Ultra-Low-RNA-seq RNA Sample Prep Kit (Illumina) and sequenced using the Illumina HiSeq 4000 instrument according to the manufacturer’s instructions for 100bp paired end read runs ...
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bioRxiv - Plant Biology 2021Quote: ... This modified version of the SMART-seq2 and SCRB-seq protocols allowed multiplexed pooling of 96 samples before library preparation with the Nextera XT DNA sample prep kit (Illumina). The protocol returned 3’-biased cDNA fragments similar to the Drop-Seq protocol (Macosko et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining batch was prepared with the TruSeq RNA Exome sample prep kit (formerly the TruSeq RNA Access sample prep kit; Illumina). All libraries were randomly pooled to multiplex 24 samples per lane using Illumina TruSeq barcodes ...
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bioRxiv - Cell Biology 2020Quote: ... data shown are from pseudo-counts-RLE obtained after RNAseq analysis of triplicate samples from RNA banks made with the TruSeq Stranded mRNA sample preparation kit (Illumina) and sequencing with a HiSeq2500 Illumina sequencer (MGX Montpellier GenomiX ...
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bioRxiv - Pathology 2020Quote: ... sequencing libraries were generated using 2 µg of total RNA per sample with the TruSeq RNA sample preparation protocol from Illumina according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The RNA-seq library of this sample was prepared using the TruSeq Stranded mRNA LT Sample Prep kit (RS-122-2101, Illumina) with 500 ng total RNA as input ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were subjected to library RT and amplification to tag the RNA molecules with specific and unique sample indexes (Illumina), followed by a final beads cleanup (1:0.8 ...
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bioRxiv - Neuroscience 2021Quote: ... We fragmented the rRNA-depleted RNA samples and processed them into strand-specific cDNA libraries using TruSeq Stranded Total LT Sample Prep Kit (Illumina) and then sequenced them on NextSeq 500 ...
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A tale of two transcriptomic responses in agricultural pests via host defenses and viral replicationbioRxiv - Genomics 2020Quote: ... The rRNA-depleted samples were used for TruSeq Stranded RNA Sample Prep kit to produce 5′ to 3′ strand-specific cDNA libraries (Illumina). A TruSeq SBS sequencing kit version 3 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... The libraries were prepared from 300 ng of RNA from each sample using TruSeq Stranded mRNA HT Kit and TruSeq Stranded mRNA Sample Preparation protocol 15031047 (Illumina). Sequencing was performed with NovaSeq 6000 SP Sequencing System (Illumina ...
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bioRxiv - Genomics 2020Quote: RNA from a subset of human fetal (n = 3) and mouse (n = 8) cortex tissue samples was prepared with TruSeq Stranded mRNA Sample Prep Kit (Illumina) and subjected to 125bp paired-end sequencing using a HiSeq2500 (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... We fragmented the rRNA-depleted RNA samples and processed them into strand-specific cDNA libraries using TruSeq Stranded Total LT Sample Prep Kit (Illumina) and then sequenced them on NextSeq 500 ...