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Citations for Illumina :
51 - 100 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 50 µL transposition reaction mix as follows: 2X Tagment DNA buffer (Illumina 15027866), 20X Tagment DNA enzyme (Illumina 15027865 ...
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bioRxiv - Genomics 2024Quote: ... nuclei were recovered with 2.5 μL of 2X TD buffer and 2.5 μL transposase (TDE1) mixture (Illumina). Following mixing by pipetting ...
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bioRxiv - Genomics 2021Quote: ... The nuclei were the resuspended in the transposition buffer (2x reaction buffer, Illumina Cat #FC-121-1030, Nextera Tn5 Transposase, Illumina Cat #FC-121-1030) and PCR amplified (KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Microbiology 2023Quote: ... v3 2x 300bp (Illumina). Demultiplexing was performed with the python package demultiplex (Laros JFJ ...
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bioRxiv - Genetics 2021Quote: ... 50,000 nuclei were incubated in the transposition reaction mix (20μl nuclease-free water; 25μl 2X Tagment DNA Buffer, Illumina Cat#FC-121-1030 ...
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bioRxiv - Neuroscience 2021Quote: ... Beads were then resuspended in 25μL of tagmentation reaction mix containing 12.5μL of 2x Tagmentation DNA Buffer (Illumina #15027866) and 1μL of Tagment DNA Enzyme (Illumina #15027865) ...
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bioRxiv - Genetics 2021Quote: ... The nuclei were resuspended in the transposition reaction mix (2x TD Buffer (Illumina Cat #FC-121-1030, Nextera), 2.5⍰μl Tn5 Transposase (Illumina Cat #FC-121-1030 ...
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bioRxiv - Immunology 2020Quote: ... the pellet was resuspended in 50 μL ATAC reaction mix (25uL 2X TD buffer, 2.5 μL Nextera Enzyme, 22.5 μL water, Illumina). The transposase reaction was carried out at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting pellet was resuspended in transposase reaction mix (25μl cold lysisl 2x TD buffer, 10μl cold lysisl transposase (Illumina), 15μl cold lysisl nuclease-free H2O ...
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bioRxiv - Genomics 2023Quote: ... DNA was tagmented by the addition of 10 µL of tagmentation mix (0.01 µL of a custom TDE1 enzyme in 9.99 µL of 2x Nexterda TD buffer, Illumina) and plates incubated at 55°C for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were then resuspended in 50 μL transposition reaction mix containing 25 μL 2X Tagment DNA buffer (Illumina), 2.5 μL Tn5 transposase (Illumina) ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were resuspended in 25uL of transposition mix (12.5ul 2x TD buffer, 1.25ul transposase (Illumina, catalog nr 20034197), 8.25ul PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The transposition reaction mix (25μl of 2X TD buffer, 2.5μl of Tn5 transposase (Illumina, San Diego, CA, USA), 15ul of PBS and 7.5μl of nuclease free water ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell pellet was then re-suspended in 50ml of transposition reaction mix (25ml 2x TD buffer (Illumina), 2.5ml transposase (Illumina) ...
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bioRxiv - Genomics 2023Quote: ... the nuclei flash-frozen in the tube strips were thawed on ice and 13.7 μl of transposition buffer (which contains 12.5 μl of 2X Illumina Tagment DNA Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclei pellets were resuspended gently in transposition reaction mix (25 µL 2X TD Buffer [Illumina Cat #FC-121-1030], 2.5 µL transposase [Illumina Cat #FC-121-1030] and 22.5 µL nuclease-free water ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei pellets were then resuspended in 50 µl of transposition mix (2.5 µl Tn5 transposase, 25 µl 2x TD buffer (both Illumina), 0.5 µl 1% Digitonin (Promega) ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was removed and transposition reaction was set up with 25 μl TD (2x reaction buffer, Nextera Kit, Illumina), 2.5 μl TDE1 (Tn5 transposase ...
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bioRxiv - Genomics 2021Quote: ... Pelleted nuclei were resuspended in 50 μL transposition mix (25 μL 2x TD Buffer (Illumina (San Diego, CA, USA) 20034197) ...
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bioRxiv - Genomics 2021Quote: ... and 5K samples were resuspended in 50 μl, 10 μl, and 5 μl of transposition mix (25 μl 2x TD buffer, 2.5 μl Tn5 (Illumina), 16.5 μl PBS (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Pellets were resuspended in 50 µL of transposition reaction (2X TD buffer, 1X PBS, 0.1% Tween-20, 0.1% Digitonin, 5µL of Illumina Tn5 transposase) and incubated for 30 min at 37°C with 1,000 rpm agitation ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... 50’000 nuclei were then pelleted at 500 RCF for 10 min at 4°C and resuspended in 50 pL transposition reaction mix containing 25 pL Nextera 2x TD buffer and 2.5 pL TDE1 (Nextera Tn5 Transposase; Illumina) (cat ...
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bioRxiv - Genetics 2023Quote: ... Nuclei were collected by centrifugation and resuspended in 50 µL transposase mix (25 µL 2x TD Buffer, 2.5 µL TDE1 transpose enzyme (Illumina Tagment DNA Enzyme and Buffer Small Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in cold PBS and tagmentation master mix (25 ul of 2x tagmentation buffer, 2.5 ul of TDE1 [Illumina] ...
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bioRxiv - Genomics 2022Quote: ... nuclei were spun at 4°C at 500g for 10 minutes and re-suspended in the transposase reaction mix (25ul 2X TD buffer, 2.5ul transposase (Illumina) and 22.5ul nuclease-free water) ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was removed and nuclei were resuspended in 50 µL of transposition mix (25 µL 2x TD buffer, 2.5 µL transposase (Nextera Tn5 transposase, Illumina), 16.5 µL PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The transposition reaction was carried out immediately afterwards using 25 µL 2x TD buffer (Illumina, Nextera DNA Library Preparation), 2.5 µL transposase (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... The transposition reaction was performed with 25 μl 2x TD buffer from and 2.5 μl transposase (100nM final) from Illumina Tagment DNA Enzyme and Buffer Small Kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... The transposition reaction was performed with 25 μl 2x TD buffer from and 2.5 μl transposase (100nM final) from Illumina Tagment DNA Enzyme and Buffer Small Kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Supernatant was very carefully removed (pellet could be almost invisible at this step) and nuclei resuspended in 45μl of transposition mix (25μl 2X TD buffer (Illumina #20034198), 16.5μl 1X PBS ...
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bioRxiv - Immunology 2024Quote: ... and 0.1% IGEPAL CA-630), washed, and right after resuspended in transposase reaction mix (25 μL 2x TD buffer, 2.5 μL transposase (Illumina) and 22.5 μL nuclease-free water ...
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bioRxiv - Genomics 2021Quote: ... Libraries were pooled equimolar to a final concentration of 4nM and 1.25 pM sample was sequenced in paired-end 2x 75 bp mode on a NextSeq 500 (Illumina) using a High-Output chip.
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bioRxiv - Genetics 2020Quote: ... The cell pellet was resuspended with 50 μl transposition mix containing 25 μl 2X TD buffer (Illumina FC-121-1030), 3.5 μl Tn5 transposase (Illumina FC-121-1030) ...
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bioRxiv - Cancer Biology 2022Quote: ... The nucleic were further resuspended in transposition mix (2X TD buffer, 1X PBS, Digitonin 0.01%, tween 20 0.1%, NFW 5ul, and Illumina transposase 2.5ul). Mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant was removed and the pellet was resuspended in 50 μl of transposition reaction mix (25 μl 2x TD buffer (Nextera DNA Library Prep Kit, Illumina), 2.5 μl TDE1 enzyme (Nextera DNA Library Prep Kit ...
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bioRxiv - Immunology 2020Quote: Permeabilized cells were pelleted for 10 minutes at 500xg and resuspended in 50 μL of transposition mix (25 μL 2x TD buffer (Illumina), 16.5 μL 1X PBS ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were then collected by centrifuging at 500 x g for 10 min at 4°C and nuclei were resuspended in 50 μL of transposition reaction mix [25 μL 2X TD buffer (Illumina), 16.5 μL PBS ...
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bioRxiv - Genetics 2023Quote: ... The nuclei was resuspended in the transposition reaction mix containing 25 μL 2x TD Buffer (Illumina, cat #FC-121-1030), 2.5 μL Tn5 Transposase (Illumina ...
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bioRxiv - Genomics 2024Quote: ... was used for a tagmentation reaction that included 3 µL of 2X TMP buffer and 0.5 µL of Transposome (BLT; CAT # 20015880, Illumina Inc.), incubated in a thermocycler at 53 ℃ for 30 minutes with the lid set at 80 ℃ ...
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bioRxiv - Genomics 2022Quote: ... We centrifuged at 500 x g for 10 minutes at 4°C and resuspended cells in the transposition reaction mix (25 µL 2X TD buffer (Illumina), 2.5 µL TDE1 Tn5 transposase (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis and DNA tagmentation was performed in a total volume of 25 µl of 2x TD (Tagment DNA Buffer) and TDE1 (Tagment DNA enzyme) (Illumina), 1% Digitonin (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... This raw nuclei suspension was then washed with RSB containing only 0.1% Tween-20 and pelleted nuclei were subject to tagmention in 50ul of transposition mix (25 ul 2X TD buffer, 100 nM TDE enzyme (Illumina), 16.5 ul PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... whole-genome and targeted libraries were added to flow cell in a 67%-33% ratio and sequenced to approximately 16M fragments per sample corresponding to ~1-2x whole genome coverage using NovaSeq S4 sequencing (Illumina) with 150 bp paired-end reads.
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bioRxiv - Genomics 2020Quote: ... the nuclei were resuspended in the transposase reaction mix (25 μL 2x TD buffer, 2.5 μL Transposase TDE1 (Nextera Tn5 Transposase, Illumina, Cambridge, UK) in a total volume of 50 μL and incubated for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated nuclei were resuspended in 50 μL of Transposition mix (Illumina Tagment DNA Enzyme 100 nM, TD-Buffer 2X (kit from Illumina, 20034197), PBS 0.33% ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the cells were precipitated and kept on ice and subsequently resuspended in 25μl 2X Tagment DNA Buffer (Illumina Nextera kit), 2.5μl Transposase enzyme (Illumina Nextera kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Then the cells were precipitated and kept on ice and subsequently resuspended in 25 μl 2X TD Buffer (Illumina Nextera kit), 2.5 μl Transposase enzyme (Illumina Nextera kit ...
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bioRxiv - Neuroscience 2024Quote: ... containing approximately 40,000 nuclei were re-suspended in the transposition mix (25μL 2x TD Buffer (Illumina Cat no. FC-121-1030), 2.5μL Tn5 Transposes (Illumina Cat no ...
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bioRxiv - Genetics 2021Quote: ... 50,000 nuclei were incubated in the transposition reaction mix (20μl nuclease-free water; 25μl 2X Tagment DNA Buffer, Illumina Cat#FC-121-1030; 5μl Tn5 Transposase, Illumina Cat#FC-121-1030) at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was discarded and the pellet (cell nuclei) was resuspended in 50 uL transposition mix (50% v/v 2X Tagment DNA (TD) Buffer (Illumina, Cambridge, UK), 32% v/v PBS ...