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Citations for Illumina :
1351 - 1391 of 1391 citations for ssc mir 432 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The PCR products (4 nM from every sample) were treated with the MiSeq Reagent Kit v2 (50 cycle format; Illumina KK, Tokyo, Japan) and sequenced using the MiSeq Sequencer (26 bp ...
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bioRxiv - Microbiology 2022Quote: Sequencing libraries were prepared from 1 ng of each VSG PCR product using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) following the manufacturer’s protocol except for the final cleanup step ...
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bioRxiv - Neuroscience 2022Quote: ... at the HIHG or The American Genome Center at Uniformed Services University of the Health Sciences (USUHS) using the TruSeq DNA PCR-Free library preparation kit and sequencing on the Novaseq 6000 (Illumina, San Diego, CA). WGS was analyzed through a bioinformatics pipeline including alignment to the GRCh38 genome build with BWA mem ...
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bioRxiv - Genomics 2022Quote: ... Libraries were created from DNA of the same HzStark_Cry1AcR male using the TruSeq DNA PCR-Free Low Throughput Library Prep Kit with TruSeq DNA UD Indexes (Illumina, San Diego, CA, USA) to yield a standard paired end library with a 350±50 bp insert size ...
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bioRxiv - Microbiology 2022Quote: ... China) for PCR amplification of the V4 region of the 16S rRNA gene and sequencing using an Illumina NovaSeq (Illumina, San Diego, CA) in two sequencing runs ...
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bioRxiv - Cell Biology 2023Quote: ... Library preparation is performed with or without amplification (Nextera® DNA Flex Library Prep kit or TruSeq® DNA PCR-Free kit, ILLUMINA®), either through automation (MICROLAB Star® ...
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bioRxiv - Genomics 2023Quote: ... The tagmentation and library preparation was performed with the Nextera DNA Library Preparation Kit with 8 cycles of PCR amplification (Illumina #FC-121-1030).
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bioRxiv - Cell Biology 2023Quote: ... Editing frequencies in samples collected following transplantation were assessed via deep sequencing of PCR amplified libraries of the CD33 gRNA target region (Table S1) on an Illumina Miseq (Illumina, San Diego, CA) via 300bp paired end reads and a median sequencing depth of 100,000 reads/gRNA target site ...
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries of individual DNA samples were prepared using the Illumina TruSeq DNA PCR-free LP kit (Illumina, San Diego, California, United States) in combination with the IDT for Illumina TruSeq DNA UD indexes kit (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... Sequencing of the PCR amplicons spanning the MHC loci was performed on the Illumina MiSeq platform using a v2 500-cycle kit (Illumina, San Diego, CA) in the FHCC Genomics Core Facility or the UCI-Fred Hutch Cancer Centre Laboratory.
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bioRxiv - Genomics 2020Quote: ... fragment libraries (average fragment sizes of ∼560 bp) were constructed using TruSeq DNA PCR-Free LT Library Prep Kit (Illumina, San Diego, CA, USA). Paired-end sequences of 150 bp long were determined using HiSeq X (Illumina) ...
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bioRxiv - Genomics 2020Quote: A total of 2 μg of intact genomic DNA was fragmented and used to construct short-insert PCR-free libraries (300 and 500 bp) following the manufacturer’s protocol (Illumina Inc., San Diego, CA, USA). The DNA libraries were used in paired-end sequencing (∼265 million paired-end reads ...
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bioRxiv - Molecular Biology 2019Quote: The extracted genomic DNA from a laboratory-reared individual was used in constructing a high-throughput sequencing library with 500-bp insert size using the Illumina TruSeq DNA PCR-Free HT Library Prep Kit (Illumina, San Diego, CA, USA). The prepared library was sequenced on an Illumina Hiseq4000 Sequencer using the Hiseq Reagent Kit v3 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... An average 350-bp paired-end libraries were prepared from extracted genomic DNA by TruSeq DNA PCR-Free High Throughput Library Prep Kit (Illumina, San Diego, CA, USA). Pair-end sequencing (2× 150-bp ...
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bioRxiv - Plant Biology 2021Quote: Libraries for PCR-free short read sequencing were prepared from MN106-Ref genomic DNA using the TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina, San Diego, CA, USA) in combination with TruSeq DNA Single Indexes Set A (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... All the libraries were prepared following the two-step PCR Illumina protocol and these were subsequently sequenced on an Illumina MiSeq instrument (Illumina, San Diego, CA, USA) using 2×251 paired-end reads for the MICROWINE samples and 2×301 paired-end reads for the Biome Makers samples.
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bioRxiv - Genomics 2021Quote: ... Eight whole genome sequencing libraries with a median insert size of 550 bp were constructed using the Illumina TruSeq DNA PCR-free sample preparation kit (Illumina, San Diego, CA, USA), according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... a paired-end metagenomic library was prepared from 100 ng of DNA using the DNA PCR free Library Prep Kit (Illumina, San Diego, CA, USA) and size selected at about 400 bp ...
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bioRxiv - Molecular Biology 2019Quote: ... and 16 PCR positive controls) was sequenced on an Illumina MiSeq® using a MiSeq Reagent Kit v3 (600-cycle) (Illumina, Inc, CA, USA). Raw sequence reads were demultiplexed using a custom Python script ...
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bioRxiv - Microbiology 2019Quote: ... the amplicon libraries underwent a second PCR reaction to attach dual indices and Illumina sequencing adapters using the Nextera XT index kit (Illumina, San Diego, CA, USA). Following purification (as described above ...
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bioRxiv - Genomics 2021Quote: ... libraries with an average insert size of 450 bp were prepared by the University of Utah’s Huntsman Cancer Institute High-Throughput Genomics Core Facility using the Illumina TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, California, USA). 2 × 150 bp reads for each parent were generated on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using a TruSeq DNA PCR-Free kit (Illumina, San Diego, CA, USA) according to Illumina library preparation protocols ...
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bioRxiv - Immunology 2022Quote: ... variable regions V3 to V4 of the bacterial 16S ribosomal RNA gene were amplified by limited cycle PCR and subjected to the Illumina 16S metagenomic sequencing library preparation workflow according to the manufacturer’s recommendations (Illumina Inc., San Diego, CA, USA). The obtained DNA libraries were quantified ...
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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bioRxiv - Genomics 2022Quote: ... 2 μg of Rohu-1 genomic DNA was used with an Illumina TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA, USA) to create an Illumina sequencing library ...
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bioRxiv - Plant Biology 2022Quote: Pair-end library preparation for MiSeq sequencing was conducted using the two-step tailed PCR method described on Illumina (Illumina, San Diego, CA, USA). We used the following primer pairs to amplify partial sequences of the 16S rRNA gene:
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... A second short-run PCR was performed in order to ligate the Illumina i7 and i5 barcodes (Nextera XT, Illumina, San Diego, CA, USA), and amplicons were purified again as above ...
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bioRxiv - Molecular Biology 2022Quote: ... A second PCR to insert Illumina adaptors (no further library preparation required) and amplicon sequencing was performed at Genewiz (Illumina-based Amplicon-EZ service).
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bioRxiv - Genomics 2023Quote: ... by the University of Wisconsin-Madison Biotechnology Center (Madison, WI, USA) as specified in the TruSeq DNA PCR-Free Reference Guide (Illumina, San Diego, CA, USA) [17] ...
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bioRxiv - Plant Biology 2023Quote: ... as per manufacturer’s instructions except that reaction volumes were scaled by one-half and 12 PCR cycles were used for library amplification (Illumina, San Diego, California, USA). Paired-end sequencing (2 × 150 bp ...
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bioRxiv - Genomics 2020Quote: ... After A-tailing and adaptor ligation fragments that carry adaptors on both ends were enriched by 8 cycles of PCR (Illumina TruSeq Nano DNA Sample Kit). The final library was quantified using PicoGreen (Quant-iT ...
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bioRxiv - Microbiology 2021Quote: ... Libraries for shotgun metagenomic sequencing were prepared using a PCR-free method at Novogene (Nanjing, China) on a HiSeq instrument (Illumina Inc. San Diego, CA, USA) with 150 bp paired-end reads and 6 Gb data/sample ...
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bioRxiv - Immunology 2020Quote: ... before fragmenting the samples to 450bp fragments and processing with the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA). Over the three-year duration of the sequencing phase of the project ...
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bioRxiv - Microbiology 2021Quote: ... and gDNA mocks were included in each PCR plate and sequenced alongside the samples sequenced with an Illumina MiSeq instrument (Illumina Inc., San Diego, CA, US) following manufacturer’s recommendations.
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bioRxiv - Microbiology 2022Quote: ... Selected samples from MPX virus-positive patients were directly sequenced without prior PCR amplification by the Illumina technology with the Nextera XT paired-end strategy on a MiSeq instrument (Illumina Inc., San Diego, CA, USA) or ...
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bioRxiv - Microbiology 2020Quote: ... A second PCR was performed to attach dual indices and Illumina® sequencing adapters using the Nextera XT Index kit (Illumina®; San Diego, CA, USA). The conditions of this PCR included ...
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bioRxiv - Microbiology 2023Quote: ... the purified PCR product was used to prepare 16S rRNA gene libraries which were then sequenced on an Illumina MiSeq instrument (Illumina Inc., San Diego, CA, United States) using the MiSeq reagent kit v3 (2 × 300 bp ...
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bioRxiv - Genomics 2024Quote: ... A pre-index anchor was ligated and a PCR amplification step with 15 cycles was done to add a 10bp unique dual index adapter sequences (IDT® for Illumina® RNA UD Indexes, Ligation). All libraries were quantified by Qubit dsDNA HS Assay measurement ...
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bioRxiv - Microbiology 2024Quote: Sequencing of genomic DNA of 21 evolved clones and the parental strain was performed on Illumina HiSeq X10 platform (Macrogen Korea, Geumcheon-gu, Seoul, Korea) with libraries prepared with Illumina-Shotgun library (Illumina TruSeq DNA PCR-free (350 bp insert)) ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR-amplified cDNA was purified using AMPure XP beads and processed with the Nextera XT DNA Library Preparation Kits (Illumina, Cat. # FC-131-1024 and FC-131-1096) as per the protocol provided by the manufacturer.