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Citations for Illumina :
1201 - 1250 of 1391 citations for ssc mir 432 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... A paired-end library with an average insert size of 450 bp was constructed with a TruSeq DNA PCR-free Library Prep Kit (Illumina) and two different mate-pair libraries (3 kb and 5 kb ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A paired-end 150 bp library was prepared for each sample using the TruSeq DNA PCR-Free Library Prep Kit (Illumina), and sequenced on the Illumina HiSeq X platform ...
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bioRxiv - Molecular Biology 2021Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... Pools were sent to the University of Geneva for sequencing Illumina (MiSeq) where sequencing libraries were prepared using the reagents of the PCR-free TruSeq kit (Illumina).
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bioRxiv - Microbiology 2020Quote: ... colonies were tested for their sensitivity to streptomycin and mutants were confirmed by PCR across the deleted region and further verified by Illumina sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2020Quote: ... Analysis of the screen is as follows: the sequences flanking retroviral insertion sites were determined using inverse PCR followed by Illumina sequencing ...
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bioRxiv - Immunology 2019Quote: ... adapter ligation and PCR amplification were performed using the TruSeq stranded total RNA with ribo-zero globin sample preparation kit (Illumina). The sequencing of transcriptome libraries was performed on the Illumina HiSeq 1500 platform (Illumina ...
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bioRxiv - Biochemistry 2019Quote: ... Libraries for Illumina Next Generation Sequencing (NGS) were produced with the two-step PCR approach pooled in appropriate ratios and analyzed by Illumina NGS.
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bioRxiv - Bioengineering 2019Quote: ... For library amplification 12 PCR cycles were applied and 45-48 libraries were pooled for sequencing on HiSeq 2000 or HiSeq 2500 instruments (Illumina) using 50 base pair single end reads.
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bioRxiv - Microbiology 2019Quote: ... Library preparation was carried out by the IBEST Genomic Resources Center at the University of Idaho (Moscow, ID): genomic DNA was used to make shotgun libraries using the TruSeq PCR-free Library Prep Kit (Illumina) with the HiSeq-length insert option (short) ...
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bioRxiv - Genetics 2021Quote: ... 700 ng of genomic DNA was sheared to an average size of 400 bp using a Covaris LE220 and was used as input to generate a whole-genome library using the TruSeq PCR-free kit (Illumina). The resulting DNA libraries were sequenced on Illumina Hi-seq X instrument to generate 2×150bp paired-end reads.
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bioRxiv - Evolutionary Biology 2021Quote: ... and subjected to a second PCR to introduce dual index sequences using Nextera XT Index Kits (Illumina, San Diego, CA). In the second PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The sgRNA composition of the population from each replicate was PCR amplified and barcoded followed by next-generation sequencing on a MiSeq (Illumina) using 150-cycle ...
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bioRxiv - Genomics 2021Quote: ... of Korea) for library preparation with 350 bp targeted insert size using TruSeq DNA PCR Free preparation kit (Illumina, USA) and sequencing on HiSeq X device (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Subsequent to second round PCR amplification MiSeq sequencing libraries were generated using Nextera XT DNA Sample Preparation Kits (Illumina, Inc.). Amplicons for each sample were tagged using Nextera XT Index Kits (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products were combined in equimolar concentrations and sequenced on an Illumina MiSeq (Miseq Reagent Kit V2, 50-cycle; Illumina) using primer PLM49 ...
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bioRxiv - Microbiology 2020Quote: ... Samples were processed for WGS using multi-segment PCR [10] and then prepared for deep sequencing using the Nextera XT library preparation reagent (Illumina). Samples were sequenced on the Illumina NextSeq platform ...
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bioRxiv - Microbiology 2019Quote: ... we performed a second PCR step to add unique dual-index combinations to each sample using the Nextera XT index kit (Illumina). Second-step PCR amplifications were performed in a total volume of 25 μl using 2.5 μl of the PCR products ...
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bioRxiv - Immunology 2020Quote: ... The transposed DNA was amplified by PCR for 10-12 cycles by using the Nextera DNA Library Preparation Kit and Nextera XT Indexing Kit (Illumina). The library DNA within the 150-to 500-bp range was enriched by AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: ... First strand cDNA was subjected to limited PCR amplification followed by Tn5 transposon-based fragmentation using the Nextera XT DNA Library Preparation Kit (Illumina). Samples were then PCR amplified for 12 cycles using barcoded primers such that each sample carries a specific combination of eight base Illumina P5 and P7 barcodes for subsequent pooling and sequencing ...
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bioRxiv - Immunology 2020Quote: ... the 2nd SGA env PCR products were cleaved into approximately 300 bp products by tagmentation using the Nextera DNA Library Prep Kit (Illumina). Indices from the Nextera Index Kit (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA library was prepared and sequenced using a TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA).
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bioRxiv - Genomics 2021Quote: ... Direct tagmentation of the mRNA/cDNA hybrids and PCR amplification were performed on beads using Nextera XT DNA Library Prep Kit (Illumina); the Reference Guide was followed exactly for 20K and 10K samples ...
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bioRxiv - Genomics 2020Quote: ... The products were then amplified by 12 cycles of PCR using specific index adapters for Illumina sequencing (Nextera XT Index Kit v2, Illumina) (Supplementary Figure 1A) ...
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bioRxiv - Genetics 2020Quote: ... Two PCR-free libraries with insertion sizes of 350 bp and 550 bp were constructed for each cat using the TruSeq DNA PCR Free library preparation kit (Illumina). The Illumina HiSeq 2000 (Illumina ...
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bioRxiv - Genomics 2021Quote: Final samples for both inverse PCR and UMI-amplicons (concentration 4 nM) were sequenced as paired-end reads on HiSeq2500 and NextSeq500 sequencers (Illumina).
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bioRxiv - Genomics 2022Quote: ... The DNA fragments cut by the enzyme were amplified by PCR and then sequenced with 2X150 bp chemistry on the Illumina HiSeq X Ten platform (Illumina).
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bioRxiv - Genomics 2022Quote: Whole-genome shotgun libraries of 13 Capsicum lines were prepared with the TruSeq DNA PCR-Free Sample Prep Kit (Illumina), in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were indexed using the Nextera XT Index Kit following the manufacturer’s instructions (Illumina, San Diego, CA, USA) and the concentration of the differently indexed samples determined using a KAPA Library Quantification Complete kit (Kapa Biosystems ...
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bioRxiv - Microbiology 2022Quote: Both the PCR products from all the four cohorts were bead purified and subjected to another round of PCR with dual indices and adapters to generate the respective libraries as recommended by Illumina. The cleaned libraries were quantitated on Qubit® flurometer and appropriate dilutions loaded on a D1000 screen tape to determine the size range of the fragments and the average library size ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was then subjected to PCR to target the 16S V3-V4 region and to generate the libraries as recommended by Illumina MiSeq sequencing (Illumina ...
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bioRxiv - Physiology 2022Quote: ... Multiplexing was conducted by ligating an 8-base molecular barcode to sequences before 15 cycles of PCR amplification and HiSeq 2500 (Illumina) Rapid Mode library sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR product was used to construct libraries and sequenced on an Illumina NovaSeq 6,000 (Illumina, San Diego, CA, USA) with 150 bp paired-end reads ...
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bioRxiv - Genomics 2022Quote: ... Sequencing libraries were prepared on 1µg of DNA using the TruSeq DNA PCR-Free Library Preparation Kit (Illumina, cat#20015963), with one additional bead cleanup performed after end-repair and after adapter ligation ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 ng of PCR1 product was subjected to another round of PCR to add unique Illumina indexes (Nextera XT Index Kit, Illumina) with KAPA Hifi Hotstart Ready Mix (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared and indexed by adapter ligation and PCR (8 cycles) using the TruSeq Nano DNA Library Prep Kit (Illumina) and TruSeq DNA Single Indexes Set A or B (Illumina) ...
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bioRxiv - Physiology 2023Quote: ... The subsequent steps of library preparation were carried out on the Agilent NGS Bravo workstation in 96-well plates adapted for the TruSeq PCR-free Sample Preparation protocol by Illumina.
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bioRxiv - Molecular Biology 2023Quote: ... an individually tagged library was prepared from 125 ng of input material using the TruSeq DNA PCR-Free kit (Illumina). Since pools of amplicons do not need to be fragmented ...
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bioRxiv - Immunology 2023Quote: ... The simultaneous antibody binding leads to hybridization of unique DNA oligonucleotides that serve as templates for polymerase-dependent extension (DNA barcoding) followed by PCR amplification and NovaSeq (Illumina) DNA sequencing as published 90 ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... we utilized an Illumina DNA Prep kit with Nextera DNA CD Indexes to create an NGS library from the purified PCR products (Illumina). The libraries were then pooled and transferred onto a MiniSeq sequencer (Illumina).
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bioRxiv - Genetics 2023Quote: ... Purified PCR products were prepared for sequencing using the standard Illumina DNA Prep protocol and reagents (Illumina DNA Prep (M), IPB) ...
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bioRxiv - Immunology 2023Quote: ... Pooled samples were purified again using the Axygen AxyPrep Mag PCR Clean-up Kit and sequenced in an Illumina MiSeq platform using MiSeq Reagent Kit v3 (600 cycle) (Illumina) at the Translational Science Center at University of New Mexico Health Sciences Center.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... We then performed a second PCR step in which unique dual-index combinations were appended to the amplicons using the Nextera XT index kit (Illumina). Second-step PCRs were performed in a 25 μL ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was amplified with 25 cycles of PCR and libraries will be prepared for sequencing with standard NexteraXT Illumina protocol (Illumina).
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bioRxiv - Zoology 2023Quote: ... DNA fragments were used to generate genomic libraries using the TruSeq DNA PCR-free library kit (Illumina, San Diego, CA), which were quality checked on an Agilent 4200 Tapestation and quantitated using a Lightcycler qPCR assay (Roche Life Science ...
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bioRxiv - Plant Biology 2023Quote: ... A paired-end library with insert sizes ranging from 450 to 550 bp was constructed using the TruSeq DNA PCR-Free Library Prep kit (Illumina) and was size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... Libraries for high-throughput sequencing of PCR products were prepared following the “16S Metagenomic Sequencing Library Preparation” protocol by Illumina, using primers with extensions containing the adapters and the MiSeq Reagent Kit v2 (CaBM_D219A_Fw and CaBM_D219A_Rv ...
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bioRxiv - Immunology 2023Quote: ... The V3-V4 region of 16s rDNA was amplified by PCR and sequenced in the MiSeq platform at THSTI (Illumina) using the 2 × 250 bp paired-end protocol yielding pair-end reads that overlap almost completely ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were then performed by PCR with a library preparation kit (Vazyme, TD501) and sequencing was done using the Novaseq system (Illumina) according to the manufacturer’s protocol.