Labshake search
Citations for Illumina :
9501 - 9550 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... transposition reaction was done with 350 rpm mixing and using the transposase from the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, San Diego, CA, USA). Libraries were quantified using KAPA Library Quantification kit (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Microbiology 2024Quote: ... The resulting lag phase RNA-sequencing library (fig. S21B) was sequenced on a NextSeq 500 instrument with a 150 cycles Mid Output Kit (Illumina, San Diego, CA, USA) in paired-end mode (Read 1 ...
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bioRxiv - Microbiology 2024Quote: ... The extracted DNA was used to construct libraries for metagenomic shotgun sequencing using the Illumina Nextera XT 1⁄4 kit (Illumina, San Diego, CA, United States). Metagenomic shotgun sequencing libraries were sequenced on an Illumina NovaSeq system (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... were checked using the Agilent-2100 Bioanalyzer System and subjected to library preparation utilizing the TruSeq stranded total RNA Gold library preparation kit with RiboZero Gold treatment and unique dual indexes according to the manufacturer’s instructions (Illumina Inc., San Diego, CA, USA). The paired-end reads of 150 bp were generated in an S4 flowcell with v1.5 sequencing chemistry on a NovaSeq-6 000 platform (Illumina Inc.) ...
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bioRxiv - Immunology 2024Quote: ... China), using Chromium Single Cell 3’ Reagent Kits v2 (from 10x Genomics, Pleasanton, CA, USA) and NovaSeq 6000 System (Illumina, San Diego, CA, USA) respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were pooled and single-end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries for Illumina sequencing were constructed according to standard protocols using the Illumina DNA prep kit and IDT 10 bp UDI indices (Illumina, San Diego, CA, USA) by the sequencing facility at SEQCENTER (PA ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from similar pooled-samples (N = 3) using the ZYMO (Irvine, CA, USA) direct-zol miniprep kit (Cat. # R2050) and sequenced using NovaSeq (Illumina, San Diego, CA, USA) paired-end (150 bp ...
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bioRxiv - Developmental Biology 2023Quote: ... ATAC-seq libraries were generated as described previously (Buenrostro, Giresi et al. 2013) using the Nextera XT DNA Library preparation kit (Illumina catalog: FC-131-1024). Sequencing was performed on an Illumina HiSeq 2500 with 30 - 40 million 75bp paired-end reads ...
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bioRxiv - Cell Biology 2023Quote: Libraries were pooled and paired-end sequenced (2X75) on the Illumina NextSeq 500 using the High output V2.5 kit (Illumina Inc., San Diego, CA, USA). Average read count for w1118 and CRTC mutant transcripts was approximately 15.3 million reads per sample ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the HMW DNA was prepared for sequencing using a Chromium Genome Linked Reads Kit (10X Genomics Inc., Pleasanton, CA) and sequenced on a HiSeq 2500 DNA Sequencing Platform (Illumina Inc., San Diego, CA). The linked-read output data were assembled using the Supernova assembler (Weisenfeld et al. ...
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bioRxiv - Genomics 2023Quote: ... Approximately one microgram of RNA from each tissue sample was utilized to prepare libraries using the TruSeq Stranded mRNA Library Preparation Kit with polyA selection (Illumina Inc., San Diego, USA). The libraries were paired-end sequenced in two separate batches ...
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bioRxiv - Synthetic Biology 2023Quote: ... libraries with insert sizes of 250 bp or greater were sequenced using 2×300 paired-end reads on an Illumina MiSeq using a MiSeq Reagent Kit v3 (Illumina Inc, MS-102-3003). Libraries with insert sizes of less than 250 bp were sequenced using 2×150 paired-end reads ...
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bioRxiv - Molecular Biology 2022Quote: ... normalised and pooled before 150 paired-end sequencing on an Illumina MiniSeq sequencing system with a Mid Output Kit (Illumina, San Diego, CA, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... and 0.5ng of each fragment mixed and used to construct barcoded sequencing libraries with the Nextera XT DNA Library Preparation Kit (Illumina, cat. no.FC-131-1096). Libraries were multiplexed and sequences were determined using Miseq 2 × 250 Illumina sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... The RNA sequencing library was generated from 300 ng total RNA using the TruSeq Stranded mRNA Library Prep kit (#20020595, Illumina, San Diego, CA, USA) with oligo-dT beads for capture of poly-A-mRNA according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA libraries were prepared for whole genome sequencing using the Tagmentation-based library prep kit according to the manufacturer’s instructions (Illumina Inc, San Diego, CA, USA). Next ...
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bioRxiv - Microbiology 2023Quote: ... The libraries were prepared using Nextera XT DNA Sample Preparation Kit and sequenced on MiSeq or NovaSeq 6000 platform (Illumina, San Diego, CA, USA). Raw reads were quality- and adaptor-trimmed using Trimmomatic v0.39 (Bolger at al. ...
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bioRxiv - Molecular Biology 2023Quote: ... rRNA subtraction was performed to deplete ribosomal RNAs from each sample using the Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria; Illumina, San Diego, CA, USA), and a TruSeq Kit mRNA Library Prep Kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were generated by Genomescan using the NEBNext Low Input RNA Library Prep Kit from Illumina (New England Biolabs, cat#E6420S/L). In short ...
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bioRxiv - Systems Biology 2022Quote: The sequencing library preparation was done using 200 ng of total RNA input with the TrueSeq RNA Sample Prep Kit v3-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275 bp fragment including adapters in average size ...
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bioRxiv - Systems Biology 2022Quote: ... single reads and 7 bases index read on an Illumina HiSeq300 instrument using the TruSeq SBS Kit HS-v3 (50-cycle) (FC-401-3002, Illumina Inc, San Diego, CA).
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bioRxiv - Genomics 2023Quote: ... The cDNA library was constructed with 50 μg RNA from each sample using the VAHTS mRNA-seq v2 Library Prep Kit (Illumina, San Diego, CA, USA). The libraries were sequenced to generate 150 bp paired-end RNA-seq reads by Biomarker Technologies Corporation (Beijing ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were pooled and sequenced single-end (1X75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).
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bioRxiv - Biochemistry 2023Quote: ... The library was prepared for sequencing from 300 ng RNA of each sample using the Illumina Truseq standard total RNA library preparation kit (Illumina, San Diego, CA, USA). The quantity and quality of amplified libraries were evaluated using Qubit 4.0 and Agilent TapeStation high sensitivity D1000 Screen Tape ...
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bioRxiv - Developmental Biology 2023Quote: ... The libraries for transcriptome analyses were prepared from 100 ng of total RNA using the TruSeq RNA Sample Preparation v2 kit (Illumina, San Diego, CA, USA) following the manufacturer’s protocols with minor modifications (Yatsu et al. ...
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bioRxiv - Genomics 2023Quote: ... The DNA sequencing libraries were constructed following the procedures provided by the Nextera XT DNA Library Prep Kit (Illumina, Inc., San Diego, CA, USA). The insert size of each library was verified by the Agilent Bioanalyzer 2100 system ...
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bioRxiv - Microbiology 2023Quote: ... 7pM of the pooled library with 30% PhiX phage as a control was sequenced using a 300-cycle Illumina MiSeq Kit (Illumina, Inc., San Diego, CA), generating 150bp paired-end reads ...
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bioRxiv - Microbiology 2023Quote: ... The RNA extracts were enriched for bacteria by depleting coral host rRNA using a Ribo-Zero Gold Yeast kit (Illumina, San Diego, CA, USA) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... Libraries were pooled and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).
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bioRxiv - Cell Biology 2022Quote: ... 75bp) on a NextSeq500 using the NextSeq 500/550 High Output Kit v2 according to the manufacturer’s instructions (Illumina Inc., San Diego, CA, USA). Analysis of RNA-sequence reads ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sequencing library with a peak insert size of 535 bp was constructed using 200 ng of genomic DNA with a TruSeq Nano LT library preparation kit (Cat # FC-121-4002, Illumina, San Diego, CA, USA) according to the kit supplier’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library with a peak span size of 1450 bp and a mean span size of 1027 bp was prepared from 570 ng of genomic DNA by tagmentation using an MP library preparation kit (Cat # FC-132-1001, Illumina, San Diego, CA, USA), without size selection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries with an insert size of 350 bp were constructed using a TruSeq Nano DNA HT Sample Preparation Kit (Illumina, San Diego, California, U.S.A.) following manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA samples were then diluted to 0.2-0.3 ng per uL and 1 ng of cDNA was carried over into library preparation using the Nextera XT DNA Library Preparation Kit (Illumina, cat. # FC-131-1096) following the manufacturer’s protocol ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... purified input RNAs and immunoprecipitated RNA fragments were sent for library construction with the VAHTS Total RNA-Seq (H/M/R) Library Prep Kit for Illumina (Vazyme, Nanjing, China) and sequencing using the Illumina HiSeq 2500 (Illumina, San Diego, CA, USA). Quality control and raw sequencing data filtration were achieved with the FastQC and fastp software ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were prepared from 1 µg of input DNA using a TruSeq DNA Sample Prep Kit according to the manufacturer’s instructions (Illumina, Inc., San Diego, CA, USA). The sheared DNA fragments underwent end-repair ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA from eukaryotic organisms was enriched using oligo(dT) beads and then rRNA was removed using the Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA, USA). First ...
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bioRxiv - Plant Biology 2023Quote: ... The final 9pM library was sequenced on an Illumina MiSeq platform using Reagent Kit v3 (2 x 300 cycles; Illumina, San Diego, CA, USA), following manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... A second round of PCR was performed with 50 ng DNA input for 8 cycles to attach Nextera XT indices and adapters using the Illumina Nextera XT Index Kit (Illumina, Catalog #FC-131-200x) and Phusion HF PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: High-quality RNA samples were used for cDNA library preparation with the Illumina Stranded mRNA Prep kit following a ligation protocol (Illumina, San Diego, CA, USA). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... and libraries were constructed by using 600 ng of total RNA from each sample and utilizing a TruSeqV2 kit from Illumina (Illumina, San Diego, CA) following the manufacturer’s suggested protocol ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from root and bud tissue using a ZYMO (Irvine, CA, USA) direct-zol miniprep kit (Cat. # R2050) and sequenced using NovaSeq (Illumina, San Diego, CA, USA) paired-end (150bp ...
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bioRxiv - Microbiology 2023Quote: ... Illumina TruSeq SBS v4 reagent kit (300 cycles) was used to generate paired-end 150 bp reads using the Illumina HiSeq platform (Illumina, San Diego CA, USA). Initial quality check was done with FastQC (v0.11.6 ...
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bioRxiv - Microbiology 2023Quote: ... a sequencing library was generated from 1 ng of the resulting SAG DNA with the NexteraXT library preparation kit (Illumina, San Diego, CA, USA) and the library was then sequenced on an Illumina MiSeq instrument (2×300 bp) ...
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bioRxiv - Microbiology 2023Quote: Phage DNA was subjected to Illumina sequencing for dsDNA by paired-end library preparation with the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... were used to initiate the TruSeq ChIP Sample Prep Kit library preparation protocol with modifications for CUT&RUN sample input (Illumina Cat# IP-202-1012). The fragmented chromatin underwent end repair and 3’ adenylation prior to Illumina indexed adapter ligation ...
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bioRxiv - Cancer Biology 2023Quote: Purified RNA was used for preparation of the sequencing library by using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions ...