Labshake search
Citations for Illumina :
9201 - 9250 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The cDNA libraries were then fragmented and amplified for sequencing using the Illumina Nextera XT DNA Library Preparation kit (Illumina, FC-131-1096) using custom primers that enabled the specific amplification of only the 3’ ends ...
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bioRxiv - Microbiology 2024Quote: We whole-genome sequenced each sample with Illumina technology (MiSeq and HiSeq 2500) using Nextera XT library preparation kits as instructed by the manufacturer (Illumina, San Diego, CA). Fastq files (raw sequencing data ...
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bioRxiv - Molecular Biology 2024Quote: ... Paired-end sequencing was carried out on an Illumina MiniSeq system according to the manufacturer’s instructions using a 300-cycle Mid or 300-cycle High kit (Illumina, San Diego, CA, USA), depending on the number of samples in each pool ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation and sequencing was performed at the QUT Central Analytical Research Facility (CARF) using the Illumina TruSeq Stranded mRNA Sample Prep Kit (strand-specific, polyA enriched, Illumina, San Diego, USA) with an input of 1 µg total RNA (RIN>9) ...
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bioRxiv - Microbiology 2023Quote: ... Short-read genome sequencing was provided by MicrobesNG (microbesng.com) where DNA was prepared using the Nextera XT kit (Illumina, San Diego, CA, USA) and sequenced on the Illumina HiSeq platform (Illumina) ...
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bioRxiv - Neuroscience 2024Quote: ... Library preparation and small RNA sequencing of the PVH and ARC samples was performed as described previously (40) using a TruSeq® Small RNA Library Preparation kit (Illumina, Cambridge, UK). Libraries were sequenced using a HiSeq 4,000 platform ...
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bioRxiv - Evolutionary Biology 2024Quote: The transcriptome was sequenced using Illumina HiSeq 2000 2x150bp platform from cDNA libraries prepared using TruSeq RNA sample prep kit v2 (Illumina, San Diego, CA) at Macrogen Inc ...
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bioRxiv - Developmental Biology 2024Quote: ... >7 passed quality control and were used for library preparation with the Illumina Stranded mRNA Library Prep Kit according to manufacturer’s instructions (Cat #20040532, Illumina Inc., San Diego, CA). This procedure includes mRNA purification and fragmentation ...
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bioRxiv - Immunology 2024Quote: ... TRB sequencing was performed on pools of libraries from 11 - 15 tumor or skin samples on the Illumina MiSeq platform using a v3 150-cycle kit (Illumina, San Diego, CA) in the FHCC Genomics Core Facility (Seattle ...
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bioRxiv - Developmental Biology 2024Quote: ... library construction was immediately carried out using a Chromium Single Cell 3’ Reagent Kit (Version 3) and sequenced on an Illumina HiSeq 4000 or an Illumina NextSeq2000 (Illumina, cat no. 20040559) by the Technion Medicine Faculty Azrieli-Technion Genomics Center ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 1 ng of cDNA per cell was transformed into a single-cell library using the Nextera XT DNA Library prep kit (Illumina FC-131-1096) and following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Microbiology 2024Quote: Total RNA of 5 μg was used to construct strand-specific RNA-sequencing libraries using the TruSeq RNA sample preparation Kit from Illumina (San Diego, CA). Briefly ...
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bioRxiv - Microbiology 2024Quote: DNA samples were sequenced at the Center for Quantitative Life Sciences at Oregon State University (Corvallis, OR) on an Illumina HiSeq platform using a Nextera XT library preparation kit (Illumina, San Diego, CA). Primer and adaptor removal and read-trimming were performed with fastP using default settings for Nextera adapters (35) ...
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bioRxiv - Microbiology 2024Quote: ... Paired-end sequencing was performed on an Illumina sequencer according to the manufacturer instructions (PairedEnd DNA sample Prep Kit – Illumina – PE-930-1001). The custom-made adapters were removed from the reads using cut-adapt ...
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bioRxiv - Microbiology 2024Quote: ... DNAse-treated RNA extracts from Large RNA fractions were processed for library preparation using the Truseq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... then the target exon was extracted with B2M exon primers with attached Nextera XT DNA Library Preparation Kit (Illumina, San Diego, Ca, USA) index adapter sequences ...
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bioRxiv - Immunology 2024Quote: ... paired-end libraries were constructed from 1 µg of the initial genomic material using the TruSeq DNA v2 Sample Prep Kit (Illumina, #FC-121-2001) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The equimolar pool of samples was spiked into a NovaSeq6000 run targeting 20M reads per sample on a S1 FlowCell (Novaseq S1 Reagent Kit, 100 cycles, Illumina, Inc, California, USA). Reads from RNA-seq were first preprocessed by Trimmomatic (v0.40 ...
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bioRxiv - Microbiology 2023Quote: ... The V3-V4 region of the 16S ribosomal RNA gene was amplified by PCR with universal bacterial primer sets (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’ and 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3’) and was sequenced using MiSeq Reagent kit v3 (600 cycle) (Illumina Inc., California, US). The sequence data were analyzed using QIIME2 (https://qiime2.org/ ...
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bioRxiv - Immunology 2023Quote: ... Approximately 1 ug of total RNA was used to remove ribosomal RNA according to the manuscript of the Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA). Following purification ...
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bioRxiv - Microbiology 2023Quote: ... the short-read sequencing was done on Illumina NextSeq 550 platform using NextSeq 500/550 Mid-Output kit v2.5 (Illumina Inc, San Diego, USA) at a commercial sequencing facility (SA Pathology ...
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bioRxiv - Microbiology 2023Quote: ... were sequenced by Next-Generation Sequencing (NGS) on the Illumina MiSeq Platform using the Illumina COVIDSeq Kit for library construction (Illumina, San Diego, USA) generating paired-end reads 150 bp long ...
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bioRxiv - Genomics 2022Quote: ... The DNA paired-end libraries were constructed using either a Nextera DNA Flex or KAPA hyper library prep kit (Illumina, San Diego, USA); the RNA paired-end libraries were constructed using a TruSeq Stranded mRNA library prep kit (Illumina ...
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bioRxiv - Immunology 2023Quote: ... 100,000 nuclei per sample were isolated using an iodixanol gradient and ATAC-seq was subsequently performed according to the original protocol (Buenrostro et al, 2013) using Nextera DNA Sample preparation kit (Illumina, FC-121-1030). After library amplification ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA-seq libraries were generated using NEB Ultra II stranded mRNA chemistry (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®), as per the manufacturers protocol with the following settings ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were prepared with a plexWell™ 96 kit (seqWell) and sequenced on an Illumina MiSeq platform (Illumina, Inc., San Diego, CA) using a v3 reagent kit yielding 2 x 301 bp paired-end reads totaling 6.4 Gbp of sequence ...
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bioRxiv - Neuroscience 2022Quote: ... Library preparation was performed with 50ng of RNA using the TruSeq PolyA+ Stranded mRNA Library Prep Kit High Throughput (Illumina, RS-122-2103). Libraries were quality-checked on a Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Molecular Biology 2023Quote: ... The pooled library was sequenced on a MiSeq Illumina platform via 2×150 nt paired-end sequencing using the 300 cycle v2 kit (Illumina, #MS-102-2002).
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bioRxiv - Microbiology 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Microbiology 2023Quote: The sequencing library was prepared using the TruSeq Stranded mRNA Sample Prep Kit and sequenced on NovaSeq 6000 (Illumina, San Diego, CA, USA), yielding more than 6G bases of sequence for each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit v2 (Illumina #RS-122-2001 and #RS-122-2002) and sequenced on NextSeq 500 using paired-end sequencing (2 x 75) ...
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bioRxiv - Genomics 2023Quote: ... The filtered nuclei were pelleted at 500 r.c.f for 5 min in a pre-chilled fixed-angle centrifuge and then resuspended in 25 μl of 1.25x Tagment DNA Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). For cell cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... of libraries were pooled and subjected to sequencing of approximately 20 million reads per sample using the Mid Output v2 kit (Illumina, FC-404-2001) on a Illumina NextSeq550 following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Illumina sequencing libraries with 6-bp single indices were constructed from 1 μg total RNA using the TruSeq Stranded mRNA LT kit (Illumina, RS-122-2101). The resulting library was validated using the Standard Sensitivity NGS Fragment Analysis Kit (AATI ...
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bioRxiv - Plant Biology 2023Quote: ... A paired-end genomic libraries with an insert size of 470 bp was prepared using the Genomic DNA Sample Prep kit (Illumina, San Diego, CA) and sequenced on an Illumina NextSeq 1000 platform ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of RNA was used as starting material for library construction with TruSeq RNA Sample Prep Kit V2 (Illumina, RS-122-2002) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality control and quantification of the resulting dual-indexed barcoded libraries was performed with Agilent TapeStation and by qPCR (NEBNext Library Quant Kit for Illumina, New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... Six hundred pg of pre-amplified cDNAs were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were prepared at the Genomics Technology Core at the University of Missouri using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA). Approximately 35 million pair-end sequences of 100 nucleotides long were produced per sample in a NovaSeq6000 instrument (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Samples with an RNA integrity number of > 9 were used for construction of a complementary DNA library by an Illumina TruSeq kit (Illumina, San Diego, CA). The sequencing strategy was 100 bp paired-end by an Illumina HiSeq 4000 ...
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bioRxiv - Genomics 2023Quote: ... The RNA library was prepared using TruSeq Stranded Total RNA Library Preparation kit with the Ribo-Zero Plant workflow (Illumina Inc., CA, USA). The transcriptome library was sequenced on Illumina NovaSeq 6000 instrument to generate 150 bp paired-end reads.
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bioRxiv - Developmental Biology 2023Quote: ... We amplified cDNA using a modified mcSCRB-seq protocol and produced libraries using the Illumina DNA Prep kit (Illumina, Inc, San Diego, CA) (112 ...
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bioRxiv - Bioengineering 2023Quote: ... Approximately 1 ug of total RNA was used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). Single-end sequencing 50bp was performed on an Illumina Hiseq 2500 at the LC Sciences (Hangzhou ...
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bioRxiv - Molecular Biology 2023Quote: ... instrument at a concentration of 300 pM with 10% PhiX using the NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles) (Illumina, San Diego, CA, USA) (paired-end ...
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bioRxiv - Molecular Biology 2023Quote: ... Healthy donor samples were sequenced using a NextSeq 500 using the NextSeq 500 High Output Kit v2.5 (75 cycles) (Illumina, San Diego, CA, USA). We loaded the library at a concentration of 2.0 pM with 10% PhiX and obtained a total of 268 M reads ...
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bioRxiv - Cancer Biology 2023Quote: ... A-tailing as well as adaptor ligation and library enrichment were performed as described in the TruSeq Stranded mRNA library preparation kit (Illumina: RS-122-2101) according to the manufacturers manual ...
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bioRxiv - Developmental Biology 2023Quote: ... 600 pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) followed by 12 cycles of library amplification ...
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bioRxiv - Genomics 2022Quote: ... 50,000 cells were resuspended in TD buffer for a 1hr incubation at 37C with TDE1 (Nexetra DNA Library Prep Kit; Illumina, San Diego, CA). Remaining steps of library prep were performed as published by Buenrostro et al ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated from 1000 ng of total RNA using TruSeq Stranded Total RNA Library Preparation Kit (Illumina, San Diego, USA, #20020596). The libraries were run on an Illumina NextSeq500 High Output 150 cycles Kit v2.5 (Illumina ...
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bioRxiv - Genomics 2023Quote: Poly-A selected stranded mRNA libraries were constructed from 1 μg total RNA using the Illumina TruSeq Stranded mRNA Sample Prep Kits (Illumina, San Diego, CA) according to manufacturer’s instructions with the following exception ...