Labshake search
Citations for Illumina :
9401 - 9450 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and were sequenced at the University of Washington Northwest Genomics Center with the Illumina NextSeq 500 High Output v2 Kit (Illumina Inc, San Diego, CA). The sequencing generated an average of 75 million base-pair paired-end raw read counts per library.
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bioRxiv - Microbiology 2020Quote: ... Next Generation Sequencing (NGS) was performed on an Illumina MiniSeq® using a Mid Output Kit (2×150-cycles) (Illumina, San Diego, CA, USA) by loading 0.8 pM on the flowcell ...
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bioRxiv - Genomics 2020Quote: ... The pooled library was quality controlled via sequencing at a concentration of 1.7 pM with 35% PhiX on a NextSeq 500 using a mid-output v2 kit (single-end 75 nucleotides, Illumina, San Diego, CA, USA), resulting in an average sequencing depth of 1 million reads ...
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bioRxiv - Genomics 2020Quote: Small RNA libraries were prepared starting from 5 µL DNase treated and spike-in supplemented RNA eluate using a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol with two minor modifications(1) ...
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bioRxiv - Genomics 2020Quote: ... Both re-pooled libraries were then sequenced at a final concentration of 1.7 pM with 25% PhiX on a NextSeq 500 using a high output v2 kit (single-end, 75 nucleotides, Illumina, San Diego, CA, USA), resulting in an average sequencing depth of 9 million reads (range 817 469 – 41.7 million reads).
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bioRxiv - Cancer Biology 2020Quote: ... 534-850ng of total RNA with DV200% varying from 38-74% was used for ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to manufacturer’s instructions with 8 cycles of PCR ...
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bioRxiv - Neuroscience 2020Quote: ... paired-end sequencing was performed on an Illumina NextSeq 500 sequencer using a 150 bp sequencing kit (v2) (Illumina Inc., San Diego, CA, USA) to produce a minimum of 50M paired-reads per library ...
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bioRxiv - Microbiology 2021Quote: ... To prepare 500 bp paired-end libraries of all isolates we used the Nextera XT DNA Library Preparation kit (Illumina, San Diego, CA, USA). Libraries were sequenced on the Illumina MiSeq sequencing platform using v2 sequencing chemistry ...
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bioRxiv - Molecular Biology 2022Quote: ... miRNA libraries were prepared from the isolated EV RNA and a water control using the TruSeq Small RNA Library Preparation Kit (RS-200-0012, Illumina, San Diego, CA, USA) using the manufacturer instructions with the modification of performing the adaptor ligation in the presence of 15% polyethylene glycol in order to reduce bias by driving the ligation reactions towards completion as determined by the Extracellular RNA Communication Consortium (46) ...
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bioRxiv - Genetics 2022Quote: ... We sequenced 80 base pair-end reads using the Illumina MiSeq platform and MiSeq Reagent Kit v3 (150 cycles) (Illumina, San Diego, CA, USA). We skipped detection of the first 17 bases of Reads 1 and 2 using the “DarkCycle” option.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Libraries were prepared using the Illumina TruSeq stranded mRNA sample preparation kit according to the manufacturer’s protocol (Illumina, Inc., Hayward, CA; RS-122-2103). Approximately 30 million 2 x 50 bp paired-end reads were collected per sample using the Illumina HiSeq2000 sequencing platform ...
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bioRxiv - Systems Biology 2022Quote: ... Paired-end sequencing was conducted using a V3 Miseq reagent kit (600 cycles) on an Illumina Miseq instrument (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Evolutionary Biology 2022Quote: Sequencing libraries were constructed from 100 ng of total DNA using the TruSeq Nano DNA LT library preparation kit (Illumina, San Diego, CA, USA) for an insert size of 350 base pairs (bp) ...
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bioRxiv - Microbiology 2022Quote: A cDNA library was prepared from RNA extracted from bat sarbecoviral isolates using the TruSeq Stranded Total RNA LT Sample Prep Kit Gold (Illumina, San Diego, CA, USA) for Rc-mk2 and Rc-kw8 strains or the MGIEasy RNA Directional Library Prep kit (MGI ...
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bioRxiv - Genomics 2022Quote: ... Barcoded libraries were pooled in equimolar amounts and sequenced as 2×300mer reads on an Illumina MiSeq platform using a MiSeq Reagent Kit v3 (Illumina, Cat#: MS-102-3003). 50% PhiX Control v3 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... For cohort I, RNA-seq libraries were generated by GenomeScan (Leiden, the Netherlands) with the TruSeq RNAseq RNA Library Prep Kit (Illumina Inc., Ipswich, MA, USA), and were sequenced using Illumina HiSeq 4000 generating ~20 million 150 bp paired end reads for each sample ...
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bioRxiv - Microbiology 2022Quote: ... Genome sequencing was performed by the Microbial Systems Molecular Biology Laboratory at the University of Michigan using an Illumina Nextera™ sequencing kit and a MiSeq (Illumina, San Diego, CA).
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bioRxiv - Genetics 2020Quote: ... and ligated to index-containing sequence adaptors using the Illumina TruSeq Stranded mRNA Sample Preparation Kit (Cat. #RS-122-2101, Illumina, Inc., San Diego, CA). The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... RNA libraries were constructed by using the RNA devoid of rRNA with the help of the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Libraries were barcoded and sequenced on a NextSeq Series Sequencing System (HUJI Center for Genomic Technologies) using Illumina flow cell (Illumina 500 NextSeq High Output v2 Kit, FC-404-2005; Illumina, San Diego, CA, USA). The sequencing data were uploaded to the usegalaxy.org (Afgan et al. ...
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bioRxiv - Systems Biology 2019Quote: ... 600 pg of each cDNA library was fragmented and amplified for sequencing with the Nextera XT v2 DNA Library Preparation kit (Illumina, San Diego, CA, USA). Single-cell libraries at 1.8 pM (final average insert sizes ranging from 580 to 680 bp ...
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bioRxiv - Systems Biology 2020Quote: ... was used to perform automated preparation of strand-specific RNA-seq libraries using the TruSeq Stranded Total RNA with Ribo-Zero Gold kit (cat# RS-122-2393, Illumina, San Diego, CA, USA). According to manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA was simultaneously fragmented and tagged with sequencing adapters in a single step using Nextera transposome (Nextera XT DNA Library Preparation Kit, Illumina, San Diego, CA, USA). Tagmented DNA was then amplified with a 12-cycle PCR ...
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bioRxiv - Evolutionary Biology 2019Quote: The Illumina TruSeq Stranded total RNA preparation kit was used to make cDNA libraries from the RNA of each individual whitefly as described by the manufacturer (Illumina, San Diego, CA, USA). Libraries were sent to Macrogen ...
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bioRxiv - Microbiology 2019Quote: ... and 1 ng/µl of DNA was used to prepare DNA libraries using the Nextera XT Library Preparation Kit and Nextera XT v2 Indexes (Illumina, San Diego, CA, USA). Multiplexed libraries were sequenced using paired end 150 bp chemistry on the NextSeq 500 NCS v2.0 (Illumina) ...
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bioRxiv - Systems Biology 2020Quote: ... 1µg total RNA was used for library preparation with the TruSeq Stranded mRNA Library Prep Kit High Throughput (Cat# RS-122-2103, Illumina, San Diego, CA, USA). Libraries were quality-checked on the Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total uterine RNA was sent to the NIH Intramural Sequencing Center to create a libarary using the TruSeq RNA Kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1µg total RNA was used for library preparation with the TruSeq Stranded mRNA Library Prep Kit High Throughput (Cat# RS-122-2103, Illumina, San Diego, CA, USA). Libraries were quality-checked on the Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Microbiology 2021Quote: Paired-end libraries were constructed with 0.2 ng/μl of purified DNA using the Nextera XT DNA Library Prep Kit as recommended by the manufacturer (Illumina, San Diego, CA, USA) and were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Shotgun metagenomic libraries were then constructed from a subset of these animals (LDC N = 15, DDC N = 7, CZMD N = 7) using the Nextera XT kit (Illumina, San Diego, CA USA) and sequenced on and Illumina HiSeq 3000 using a 150bp PE sequencing kit (Illumina).
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bioRxiv - Microbiology 2020Quote: ... the PCR amplicons were pooled and subjected to library preparation for Next Generation Sequencing using the Nextera XT library prep kit (Illumina, San Diego, CA, USA). The library was normalized and sequenced using a MiSeq nano v2 2×250 sequencing kit ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina, San Diego, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Shotgun metagenomics and isolates genome sequencing was performed on the NextSeq 500 using NextSeq High Output v2 Kit (300 Cycles) (Illumina, San Diego, CA, USA) by the Sequencing Core Facility at The Novo Nordisk Foundation Center for Biosustainability (Technical University of Denmark ...
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bioRxiv - Microbiology 2021Quote: ... The trimmed DNA fragment sequences were then used to prepare a shotgun paired-end library with an average insert size of 350 bp using a TruSeq DNA Sample Prep kit (Illumina, San Diego, CA, USA). The library was sequenced on an Illumina MiSeq platform (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used 2 μg total RNA for library preparation following the protocol in the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). The samples were sequenced by single-end reads (75 bp ...
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bioRxiv - Genetics 2021Quote: ... The thirty-seven sequencing libraries were created by reverse-transcription from 2 µg of RNA from each sample using the TruSeq Stranded mRNA Library Prep kit (Illumina, San Diego, CA, USA). Index adapters were added to identify sequences for each sample in the final data ...
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bioRxiv - Molecular Biology 2019Quote: Strand-specific RNA-sequencing (ssRNA-seq) libraries were prepared using the illumina Truseq Stranded mRNA Sample Preparation Kit (Illumina, catalogue number RS-122-2101) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... 96 libraries were pooled and sequenced in 4 runs using NextSeq 500 High75 Output kits on an Illumina NextSeq 500 instrument (Illumina, San Diego, CA, USA). Reads were pre-processed and mapped using BaseSpace SecondaryAnalysis (version 2.4.19.6 ...
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bioRxiv - Plant Biology 2019Quote: ... Library preparation was performed on 1 μg of high quality RNA (RNA integrity number equivalent superior or equivalent to 8.5) using the TruSeq Stranded mRNA Library Prep Kit High Throughput (Illumina cat. no. RS-122-2103) for ‘Cristobalina’ ...
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bioRxiv - Developmental Biology 2019Quote: ... Approximately 5 ug of total RNA was used to deplete ribosomal RNA according to the manuscript of the Ribo-Zero™ rRNA Removal Kit (Illumina, San Diego, USA). After removing ribosomal RNAs ...
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bioRxiv - Molecular Biology 2019Quote: ... the libraries were sequenced on a single MiSeq run using an Illumina MiSeq Reagent Kit v2 for 2 × 150 bp PE (Illumina, San Diego, CA, USA). All sequencing reads obtained in the present study were deposited in the DNA Data Bank of Japan (DDBJ ...
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bioRxiv - Genomics 2021Quote: ... 2 × 150 bp reads for each parent were generated on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina, San Diego, California, USA). A total of 539,570,835 reads for the N ...
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bioRxiv - Genomics 2021Quote: ... and 2 × 150 bp reads were produced on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina, San Diego, California, USA). An average of 57,521,215 reads were generated for each of the 4 samples ...
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bioRxiv - Genomics 2021Quote: ... We then added adapters and barcodes onto the ends of the fragmented DNA to generate indexed sequencing libraries using TruSeq Library Preparation Kit (Illumina, San Diego, CA, U.S.). We performed capture-based target enrichment using the SeqCap EZ Hybridization and Wash Kit (Roche NimbleGen ...
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bioRxiv - Microbiology 2021Quote: ... and the pooled DNA was used for the preparation of sequencing libraries using the Nextera XT DNA library prep kit (Illumina, San Diego, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA libraries from all samples were then prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 100-500 ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
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bioRxiv - Genomics 2021Quote: ... For long read sequencing (Oxford Nanopore Technologies) total RNA was extracted as described above and depleted using the Ribo-Zero rRNA Removal Kit Plant Leaves (Illumina, San Diego, CA, USA). RNA was then purified and concentrated on a RNA Clean Concentrator™-5 column (Zymo Research ...
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bioRxiv - Genomics 2021Quote: Six Illumina RNA-Seq libraries (one for each organ) were constructed from 500ng total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows for mRNA strand orientation (the orientation of sequences relative to the antisense strand is recorded) ...
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing was performed using the NextSeq 500/550 High-Output v2 (150 cycle) Kit (# FC-404-2002) on the NextSeq 550 platform (Illumina Inc, #SY-415-1002). Libraries were combined in an equimolar pool based on the library quantification results and run across 5 High-Output Flow Cell v2.5.