Labshake search
Citations for Illumina :
9001 - 9050 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... High-quality sequencing libraries were constructed by following the protocol of IlluminaTruSeq™ (cat. no. FC-121-2003) DNA preparation kit (Illumina, CA, USA), then were sequenced on Hiseq2000 platform (Novogene ...
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bioRxiv - Immunology 2021Quote: ... 384 purified samples were pooled into one library and then subjected to paired-end sequencing using Illumina MiSeq Nano 300 V2 cycle Kits (Illumina, #MS-103-1001) at a concentration of 12 pM.
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bioRxiv - Immunology 2021Quote: ... Six hundreds pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification ...
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bioRxiv - Immunology 2021Quote: ... 62 samples including the mock community were pooled at equimolar concentrations and the library was sequenced using a MiSeq sequencer with 2 x 250 bp paired-end run (Illumina MiSeq, v2 kit). The resulting sequence data was processed using ‘Quantitative insights into microbial ecology 2’ (QIIME2 version 2020.2 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once with PBS before DNA transposition was performed with the Nextera DNA Library Prep Kit (Illumina, Cat. #FC-121-1031). Per sample ...
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bioRxiv - Immunology 2021Quote: ... greater than 8.0 were used to generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep Kit with TruSeq Unique Dual Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Two sex-specific DNA Pool-Seq libraries were prepared for each species using the Illumina TruSeq Nano DNA HT Library Prep Kit (Illumina, San Diego, CA) with the same protocol as for the draft genome sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... RNA library from homecage and fear conditioned mice were prepared at the PGFI sequencing core at the University of Pennsylvania using the TruSeq sample preparation kit (Illumina San Diego, CA) according to the manufacturer’s instructions with polyA selection ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The DNA library was prepared using the Nextera XT library preparation kit following the recommendations of the manufacturer (Illumina, San Diego, CA, USA). DNA library was sequenced on the Illumina Miseq platform using a paired-end approach of 75 bases with Reagent Kit V3 of 150 cycles.
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bioRxiv - Bioengineering 2021Quote: Three replicates of rat skin wounds in each group were detected for the assay of Bulk RNA sequencing (NEBNext® Ultra™ RNA Library Prep Kit for Illumina®), and the results were analyzed for Gene Ontology (GO ...
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bioRxiv - Neuroscience 2020Quote: ... was used for cluster generation and sequencing was performed on a Illumina HiSeq 2500 single end 50 bp using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Microbiology 2021Quote: Bacterial 16S V3-V4 regions were amplified by PCR using universal primers derived from DBact-0341-b-S-17 and S-D-Bact-0785-a-A-21 (24) containing unique adapters for the Nextera XT indexing kit according to the manufacturer’s instructions (Illumina Inc., San Diego, CA). Fungal ITS2 regions were amplified using separate unique adapter primers derived from IST3_KYO1 and IST4_KYO1 (25) ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... Samples were confirmed to have sufficient quality with Agilent Bioanalyzer before undergoing library preparation by Tufts University Genomics Core using the TruSeq stranded mRNA kit (20020594; Illumina; San Diego, CA). Samples were run on a HiSeq2500 platform at a depth of 50 million single-end 50 base pair reads to allow for transcript quantitation64 ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 0.4 - 1 μg of total RNA were used to prepare small RNA cDNA libraries utilizing the specified protocol for the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, USA). Single-read sequencing of 50 bp was performed on an Illumina Hiseq 2500 at LC Sciences LLC (Houston ...
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bioRxiv - Cell Biology 2021Quote: ... The libraries were added in equal molecular amounts and were sequenced on an Illumina Next-seq DNA sequencer with a 75-bp pair-end cycle sequencing kit (Illumina, San Diego, CA). The detected reads were analyzed using CLC Genomics Workbench software (ver.8.01 ...
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bioRxiv - Neuroscience 2020Quote: ... Library preparation for RNA-Seq was performed using the TruSeq™ RNA Sample Prep Kit v2 (Illumina, Cat. N°RS-122-2002, USA) starting from 50 ng of total RNA ...
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bioRxiv - Neuroscience 2020Quote: ... Library preparation was performed by capture of sequence-specific coding RNA using 40 ng of total RNA and the TruSeq RNA Access Library Prep Kit (Cat No RS-301-2001, Illumina, San Diego, USA). Libraries were sequenced as paired-end 68 bp reads on a HiSeq2000 (Illumina ...
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bioRxiv - Neuroscience 2019Quote: ... One ng of the pooled amplicons was used for library construction with the Nextera XT DNA library preparation kit (#FC-131-1096; Illumina, San Diego, CA), according to the manufacturer’s guidelines ...
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bioRxiv - Genomics 2020Quote: ... Approximately 500 μg of total RNA was used as input for the TruSeq Stranded mRNA Library Prep kit (Illumina, Inc., San Diego, CA); and library preparation was carried out following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA from infected barley leaves at 6 days post-inoculation (dpi) was isolated by using the MasterPure™ Complete DNA&RNA purification Kit (Epicentre®, Illumina®) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... and libraries were constructed using 600 ng of total RNA from each sample and the TruSeqV2 kit from Illumina (Illumina, San Diego, CA) following manufacturers suggested protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were prepared from single frond samples using 1μg starting RNA using the TruSeq RNA library prep kit V2 (Illumina Systems, San Diego, USA) and quantified by KAPA library quantification (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... One μg of DNase-treated total RNA was used for library construction using TruSeq Stranded mRNA Library Prep Kit (Illumina Inc, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The consortium metagenomic library was prepared with 200 ng of starting material using the TruSeq DNA Nano kit (Illumina, San Diego, CA, USA) and paired-end sequenced (2×100 bp ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA library preparation was performed using 1μg of total RNA with a TruSeq Stranded mRNA Library Preparation Kit High Throughput (Illumina, San Diego, California, USA) according to manufacturer’s instructions (Catalog # RS-122-9004DOC ...
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bioRxiv - Neuroscience 2020Quote: ... indexing and amplification of the transposed DNA samples was performed by combining 10 µL of transposed DNA with the following: 5 µL of the Nextera i5 and i7 indexed amplification primers (Nextera Index Kit, Illumina, FC-121-1011), 25 μl of the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Goe13 and lysogens and sequenced with the MiSeq system and reagent kit V.3 (2 x 300 bp) (Illumina, San Diego, CA, USA) and the NovaSeq system (2x 150bp ...
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bioRxiv - Microbiology 2021Quote: ... The sequencing amplicon pools were diluted to 0.2 ng/µl and tagmented with Nextera XT library prep kit (Illumina, Cat#FC-131-1024). Nextera libraries were dual-barcoded and sequenced on an Illumina NextSeq1000 instrument ...
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bioRxiv - Microbiology 2021Quote: Paired-end DNA and cDNA libraries (2 × 150 bp) were constructed using TruSeqTM DNA Sample prep kit (Illumina Inc., San Diego, CA, USA) and TruSeqTM RNA Sample prep kit (Illumina Inc. ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was sent to Beijing Genomics Institute for library prep with the TruSeq small RNA sample preparation kit (Illumina, San Diego, California) and sequencing by 50 bp single end sequencing with the BGISEQ-500 platform ...
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bioRxiv - Microbiology 2021Quote: ... Preparation of cDNA libraries was performed according to the manufacturer’s instructions for the TruSeq stranded mRNA kit (Illumina, San Diego, CA, United States). Subsequently ...
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bioRxiv - Microbiology 2021Quote: Total RNA from tissues was converted to cDNA with SuperScript IV and sequencing libraries prepared with the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024). Sequencing was performed using the Illumina NextSeq platform with 150bp paired-end reads ...
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bioRxiv - Microbiology 2019Quote: ... Tagged libraries were pooled and sequenced (300 cycles, paired-end sequencing) in the Illumina HiSeq 4000 instrument using a HiSeq 4000 SBS Kit (Illumina, FC-410-1003). Raw reads were preprocessed using the standard Illumina pipeline to segregate multiplexed reads.
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bioRxiv - Microbiology 2020Quote: ... For all 2018 samples sequencing was performed using an Illumina MiSeq and the MiSeq Reagent Kit v2 (Illumina, Inc., San Diego, CA, USA) for 2 sets of 250 cycles ...
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bioRxiv - Microbiology 2020Quote: ... One µg total RNA from each sample was used to generate a paired-end RNA-seq library by polyA mRNA workflow using TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA). Each library was subjected to sequence in Illumina Novaseq 6000 (2 × 150bp read length ...
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bioRxiv - Cancer Biology 2020Quote: ... All the cell line mRNA libraries for sequencing were prepared according to the TruSeq Stranded Total RNA Library Prep Kit (Illumina, #RS-122-2201). Sequencing (75 bp ...
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bioRxiv - Cancer Biology 2020Quote: ... University of Pittsburgh sample libraries were manually prepared via standard protocols using the Illumina TruSeq RNA Exome Library Prep Kit (Illumina, San Diego, CA) and sequenced on an Illumina NextSeq sequencing system using NextSeq 500 Mid- and High-Output 150 cycle kits (75 bp ...
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bioRxiv - Cancer Biology 2020Quote: ... and sequenced on an Illumina NextSeq sequencing system using NextSeq 500 Mid- and High-Output 150 cycle kits (75 bp, paired-end; Illumina, San Diego, CA). Quality control was performed on the raw reads using RNA-SeQC (v1.1.7) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.6 ng of the amplified cDNA was converted into the sequencing library with the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024), according to the protocol supplied ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing were performed on the Illumina HiSeq 4000 paired end at 2x 100 bp (2x at least 40 million reads) using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Microbiology 2021Quote: ... Total RNA was normalized to 100 ng prior to random hexamer priming and libraries generated using the TruSeq Stranded Total RNA - Globin kit (Illumina, San Diego, USA). Libraries were assessed on the Fragment Analyzer using the High Sense Large Fragment kit and quantified using a Qubit 3.0 fluorometer (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted libraries were quantified using a Qubit dsDNA HS Assay Kit and sequenced on a NextSeq 500 or Hi-Seq 4000 (Illumina, San Diego, California). A list of the RNA-Seq samples can be found in Supplementary Table 5.
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were converted to RNA-seq libraries with the TruSeq Stranded Total RNA Library Preparation Gold kit (Illumina, San Diego, CA, USA). This kit was reported to reliably and reproducibly generate libraries from 1-2 ng input RNA (Schuierer et al. ...
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bioRxiv - Cell Biology 2021Quote: ... After chemical fragmentation by incubating for 15 min at 94°C the sample was directly subjected to the workflow for strand specific RNA-Seq library preparation (NEBNext® Ultra™ RNA Library Prep Kit for Illumina®). For ligation custom adaptors were used (Adaptor-Oligo 1 ...
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bioRxiv - Cell Biology 2021Quote: ... The mRNA was subsequently fragmented and used for cDNA synthesis and library preparation using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, USA) according to the manufacturer’s instructions ...
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bioRxiv - Paleontology 2022Quote: ... DNA extracted from mammoth and sloth boluses were sequenced on an Illumina MiSeq using the 600-cycle v3 kit (Illumina, MS-102-3003), whereas the elephant and modern and paleontological bison samples were sequenced on an Illumina NextSeq 500 system ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... RNA-seq libraries were prepared from total RNA (≥200 nucleotides in length) using the TruSeq Stranded Total RNA Ribo-Zero H/M/R kit (Illumina, RS-122-2201). Libraries were paired-end sequenced on a NextSeq 500 (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were sequenced using a custom sequencing primer (GCGACCACCGAGATCTACACACTGACTGCAGTCTGAGTCTGACAG) and the NextSeq® 500/550 Mid Output Kit v2 - 150 cycles (FC-404-2001, Illumina, CA, USA) on the Illumina NextSeq® platform.
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bioRxiv - Cancer Biology 2022Quote: Total RNA libraries (COR-L279) and mRNA libraries (H446 and LX22) were constructed using a TruSeq Stranded Total RNA Kit (Illumina, RS-122-2201) and an mRNA Library Prep kit V2 (Illumina ...