Labshake search
Citations for Illumina :
8951 - 9000 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... A total amount of 3μg RNA per sample was used for mRNA purification and library construction via the Truseq™ RNA Sample Prep Kit (Illumina, CA, USA) followed the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... libraries were prepared from 1 ng of cDNA using the Nextera® XT DNA Library Preparation Kit (Illumina Inc., San Diego, CA USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... both ribosome footprinting and total RNA samples were depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (H/M/R) (Illumina, catalog no. MRZG126) with the modification that the 50°C incubation was not performed for the ribosome footprinting samples ...
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bioRxiv - Developmental Biology 2020Quote: ... prior to pooling and sequencing using the Illumina NextSeq 500 platform using a custom primer and the High Output v2 kit (75 cycles) (Illumina, #FC-404-2005). The library loading concentration was 2.2 pM and sequencing configuration as following ...
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bioRxiv - Developmental Biology 2020Quote: ... 500 ng of total RNA was used for each RNA-seq library preparation using a TruSeq Standard mRNA kit (Illumina, San Diego, CA) as described in our previous publications (Khristi et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... This nuclear pellet was used to prepare ATAC-seq libraries using the Nextera DNA Library Preparation Kit (Illumina, San Diego, CA, Cat #FC1211030). The pellet was suspended in 5 μL buffer TD before adding 2.5 μL water and 2.5 μL tagment DNA enzyme ...
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bioRxiv - Developmental Biology 2021Quote: Approximately 50,000 nephron progenitor cells were used to generate each sequencing library for the assay for transposase-accessible chromatin (ATAC-seq) using the Nextera DNA Flex Library Prep Kit (Illumina FC-121-1030) with modifications according to a previously published method (Buenrostro et al. ...
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Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomesbioRxiv - Genomics 2020Quote: ... RNA libraries were then prepared for shotgun transcriptome sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA) as previously described.12,13,14 Libraries were then sequenced using the NovaSeq SP Reagent kit (2 × 150 cycles ...
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bioRxiv - Genomics 2020Quote: ... The remaining 74 paired-end short insert (350 nt) sequencing libraries were constructed using 100 ng sample DNA and the TruSeq DNA Nano kit (Illumina, San Diego, CA) with coverage between 8-40x (Kitchen et al ...
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bioRxiv - Genomics 2020Quote: ... Multiple adapters were used for pooling libraries (KAPA Single-Indexed Adapter Kit KK8700 and NEBNext® Multiplex Oligos for Illumina NEB #E7535S/L). The DNA samples were sequenced using the Illumina NextSeq 500 platform and 150-nucleotide single-end reads were generated ...
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bioRxiv - Genomics 2022Quote: ... One ng of the pooled amplicons was used for library construction with the Nextera XT DNA library preparation kit (#FC-131–1096; Illumina, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were resuspended in 25 μl of 2X TD Buffer and combined with Tn5 enzyme from the Nextera DNA Sample Preparation Kit (Illumina, FC-121-1030) and water to final volume 50 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2.5 µl of each Nextera Index 1 (N7XX) and Nextera Index 2 (N5XX) primers from a Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011). PCR was performed according to the following protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Granulosa cells were lysed and tagmentation was performed on the nuclei using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, USA). DNA purification was performed using the Monarch PCR & DNA Cleanup Kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting library was diluted to 10-pM in 600-μL of HT1 hybridization buffer (Illumina Nextera XT kit Cat#FC-131-1024) and 10-μL was loaded onto a 300-cycle MiSeq Nano v2 flow cell (Illumina Cat#MS-102-2002 ...
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bioRxiv - Molecular Biology 2020Quote: ... Oxford Nanopore Technologies’ Direct RNA sequencing (DRS) libraries were constructed as follows: intracellular RNA was immediately ribo-depleted using Ribo-Zero kits (Illumina, San Diego, CA) upon RNA extraction and used within 24 hours of ribodepletion (stored on ice) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA quality control was performed in a fragment analyzer and sequencing libraries were constructed using a Nextera DNA Flex kit (now renamed to Illumina DNA Prep, Illumina, San Diego, CA) and 2×150 BP paired-end sequencing was performed in an Illumina NextSeq500 ...
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bioRxiv - Molecular Biology 2019Quote: ... Genomic DNA from approximately 15-20 progeny per mutant were pooled and sequencing libraries were prepared with a Nextera kit (Illumina, FC-121-1030). All whole genome sequencing data is available on NCBI SRA (accession #PRJNA526508) ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA was extracted from Bergerac and N2 control lines as previously described (Konrad et al. 2018) with libraries prepared using the Nextera DNAflex library kit (Illumina, San Diego, CA). Libraries were sequenced on the Illumina Novaseq6000 platform (2×150 bp ...
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bioRxiv - Genomics 2019Quote: For the bacterial DNA libraries for sequencing on Illumina platform were prepared applying Nextera XT DNA Library Preparation Kit (Illumina, San Diego, USA) with small modifications (Baym et al. ...
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bioRxiv - Genomics 2020Quote: ... The dissolved DNA was then tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... The 300 ng gDNA of all four samples were used to prepare paired-end libraries with the Nextera ™ DNA Flex Library Preparation kit with an average insert size of 600 bp for all four samples according to the manufacturer’s instructions (Illumina Inc., San Diego, CA).
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bioRxiv - Genomics 2020Quote: ... Approximately 1 μg of amplified DNA was sequenced by our core sequencing facility with a 600-cycle MiSeq Reagent Kit v3 (Illumina, MS-102-3003) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and libraries were constructed by the Lexogen QuantSeq 3’ mRNA-Seq Library Kit FWD (Lexogen, Vienna, Austria).19 All RNA libraries were sequenced by the Illumina HiSeq4000 (Illumina, San Diego, CA). Raw sequencing data were aligned to the reference genome (GRCh37 ...
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bioRxiv - Genomics 2020Quote: ... we first generated pseudo-microarray variant calls from GIAB benchmark variants of HG002 and HG005 in the benchmark regions by extracting the variant sites used by a popular commercial microarray kit (Illumina Infnium OmniExpress-24). We obtained the microarray sites from the CSV manifest file (GRCh38 version ...
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bioRxiv - Molecular Biology 2021Quote: ... The library was prepared with PCR products pooled in equimolar amounts following the manufacture’s protocol and loaded in a Micro MiSeq Reagent Kit v2 (500-cycles) (Illumina, San Diego, CA, USA) on a MiSeq platform (Illumina) ...
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bioRxiv - Microbiology 2019Quote: ... 2 µL of each P7 and P5 of Nextera XT Index Kit v2 index primers (Illumina Catalogue No. FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Genomics 2019Quote: ... Four libraries were paired-end sequenced (75 nt each) with the NextSeq 500 using the Mid Output v2 kit (150 cycles) (Illumina, #FC-404-2001).
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bioRxiv - Genomics 2019Quote: ... prior to pooling and sequencing using the Illumina NextSeq 500 platform using custom ReadOne primer (IDT) and the High Output v2 kit (75 cycles) (Illumina, #FC-404-2005). The library loading concentration was 2.2 nM ...
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bioRxiv - Plant Biology 2019Quote: ... Libraries for RNA expression analysis were prepared in duplicates from 2 μg of total RNA using the TrueSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: 1 µg of total RNA from each sample was used for RNA sequencing library preparation with the TruSeq® Stranded mRNA Library Prep kit (Illumina, catalog # 20020594). The libraries were barcoded by TruSeq® RNA Unique Dual Indexes (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: ... and ∼1 ug of each sample was provided to the Genome Sequencing Core at the University of Kansas for library preparation using the TruSeq stranded mRNA kit (Illumina, San Diego, CA). Single Read 100 (SR100 ...
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bioRxiv - Cancer Biology 2019Quote: ... mRNA sequencing libraries were generated from 50 ng total RNA using the Illumina Neoprep TruSeq stranded mRNA library prep kit (Illumina, NP-202-1001). RNA libraries were sequenced paired-end (2 × 75 bp ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were pooled and sequenced (paired-end 2*75bp) on a NextSeq500 using the NextSeq 500/550 High Output Kit v2 according to the manufacturer’s instructions (Illumina, San Diego, CA, USA). Raw sequencing data generated by the Illumina NextSeq500 instrument were mapped to the mouse reference genome using the hisat2 software(Kim et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... A RIP-seq complementary DNA library was prepared from 1 µg of total RNA using the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... A total of 1 ng of high-quality genomic DNA was used as input material for library construction using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). Libraries were normalized using the bead-based normalization procedure (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... RNA-Seq libraries were prepared with the Illumina standard mRNA-seq library preparation kit and sequenced on a HiSeq 2500 system (Illumina, San Diego, CA) with paired-end mode.
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bioRxiv - Cancer Biology 2019Quote: ... Sequencing libraries prepared from enriched DNA using the ThruPLEX-FD Prep Kit (Rubicon Genomics, Ann Arbor, MI) and were sequenced using 75-base pair single reads on the Illumina platform (Illumina, San Diego, CA) at the Dana-Farber Cancer Institute ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments underwent an NGS library preparation procedure consisting in end repair and methylated adaptor ligation using the TruSeq DNA Sample Preparation kit (Illumina, San Diego, CA). Adapter-ligated fragments ranging from 200 to 300 nucleotides were isolated by agarose gel electrophoresis (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The multiplexed sequencing library for the Illumina platform was prepared using the Nextera kit (Illumina catalog #FC-121-1031 and #FC-121-1012) and a modified version of the Illumina-recommended protocol [68] ...
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bioRxiv - Genetics 2019Quote: ... total RNA from cochleae was reverse transcribed with random primers after ribo-depletion using a TruSeq library kit (15031048 Rev. C, Illumina, San Diego, CA), and 2×93bp sequenced on an Illumina HiSeq1500 instrument ...
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bioRxiv - Genetics 2019Quote: ... and sequencing reagents were used throughout the process using NEBNext Ultra DNA Library Prep kit following the manufacturer’s recommendations (Illumina, San Diego, CA, USA). End repaired adapters were ligated after adenylation of the 3’ends followed by enrichment by limited cycle PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA-seq libraries were constructed using SENSE Total RNA-seq Library Prep Kit v2 (Lexogen, Vienna, Austria) following the manufacturer’s protocol and sequenced on NextSeq 500 (Illumina, San Diego, CA, USA) with 75 nt read length ...
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bioRxiv - Genomics 2019Quote: ... the nuclei pellets were resuspended in transposase Master Mix (1.25 μl 10x TD buffer, 5 μl H2O and 6.5 μl of Tn5: Illumina Nextera Kit; FC-121-1031) and incubated for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... were sequenced at a 300-bp paired-end-read using the Nextera XT library preparation kit at the MiSeq platform (Illumina, San Diego, CA). De novo assembly was achieved using CLC Genomics Workbench 10.1.1 (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... Sequencing were performed on the Illumina HiSeq 4000 single end 125 bp using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Genomics 2020Quote: ... Sequencing was undertaken at UCD Conway Institute of Biomolecular and Biomedical Research on an Illumina NextSeq 500/550 using the high output v2 (75 cycle) reagent kit (Illumina TG-160-2005).
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bioRxiv - Genetics 2021Quote: ... Libraries constructed with the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen, Greenland, NH) were sequenced on an Illumina NextSeq 500 (Illumina, San Diego, CA) at the Genomics Facility of the Cornell Institute of Biotechnology.
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bioRxiv - Genetics 2021Quote: ... homokaryotic mutants were crossed to a genetically polymorphic Mauriceville strain and approximately 15-20 antibiotic-resistant progeny were pooled and prepared for whole genome sequencing using the Nextera kit (Illumina, FC-121-1030). Mapping of the critical mutations was performed as previously described (Hunter 2007 ...
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bioRxiv - Genomics 2021Quote: RNA-Seq library preparation was carried out from 1 µg total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation ...