Labshake search
Citations for Illumina :
8701 - 8750 of 9681 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... A total of 2 ng of DNA from each sample was used for library preparation using the Nextera reagent kit (Illumina Inc., USA). All libraries were checked for quality using the Bioanalyzer system prior to sequencing ...
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bioRxiv - Genomics 2019Quote: ... according to the manufacturer’s instructions and sequenced on the Illumina NextSeq 550 using the High Output v2 kit (Illumina, TG-160-2004).
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bioRxiv - Microbiology 2020Quote: ... Paired-end 150 bp sequencing was performed for each library on a MiniSeq with the 300-cycle mid-output reagent kit (Illumina, https://www.illumina.com), multiplexed with targeted sampling of ∼40,000 clusters per library ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were sequenced using a 150 bp paired-end NextSeq High Output V2 reagent kit (Illumina, FC-404-2004, San Diego, CA). Illumina short reads were assembled into contigs in two steps ...
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bioRxiv - Molecular Biology 2019Quote: ... 2.5 µL Tn5 transposase and 22.5 µL nuclease-free H2O) using reagents from the Nextera DNA library Preparation Kit (Illumina #FC-121-103). Samples were then incubated at 37°C for 30min ...
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bioRxiv - Cancer Biology 2019Quote: ... and 513ng-1µg of total RNA with an RNA integrity number ranging from 1.3 to 8.3 underwent ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to instructions provided by the manufacturer with 8 cycles of PCR ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted from blood leukocytes for each member of the family trio and genomic libraries were prepared using 1.2 μg of DNA with the TruSeq DNA sample preparation and Exome Enrichment kit (62Mb; Illumina, San Diego, CA). Sequencing was performed by paired-end sequencing on a HiSeq2000 instrument (Illumina Inc ...
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bioRxiv - Genetics 2021Quote: ... Diluted libraries were then sequenced on High or Mid capacity 150 bp sequencing kits on the Nextseq 500 system following manufacturer’s recommendations (Illumina Inc. 20024907, 20024904). For greater sequencing effort ...
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bioRxiv - Genomics 2021Quote: ... Library preparation was performed with 100 ng of RNA using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, USA) according to the manufacturer’s instructions (Part #15031047 Rev ...
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bioRxiv - Microbiology 2020Quote: ... Library preparation with 1 ng of extracted DNA was done by applying the Nextera XT DNA Library Prep Kit according to the manufacturer’s instructions (Illumina, San Diego, CA). Libraries were subjected to whole-genome sequencing using an Illumina® MiSeq benchtop devise (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: The M-RTPCR amplicon libraries were prepared using the Nextera XT DNA library preparation kit and protocol (Illumina, San Diego, California, USA), cleaned using the 0.8× AMPure XP beads ...
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bioRxiv - Genomics 2021Quote: ... Separate libraries were prepared from the two RNA pools using the TruSeq Stranded mRNA LT sample prep kit (Illumina, San Diego, CA). Libraries were run in paired-end 150-base format on both lanes of a dual flow cell in Rapid Run mode on an Illumina HiSeq 2500 System ...
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bioRxiv - Genomics 2020Quote: ... 2000 and underwent a 50 cycle single read sequence run with TruSeq SBS Kit v3-HS reagents (Illumina, Cat. FC-401-3002). The raw sequence reads were aligned to the reference genome using STAR (version 2.7.3a) ...
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bioRxiv - Microbiology 2021Quote: ... cDNA libraries were prepared using 100 ng of total RNA according to the manufacturer’s instructions for the Illumina TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero Gold (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Microbiology 2021Quote: ... a unique indexed sequencing adapter was added to each sample using the Nextera XT Index kit v2 (Illumina, San Diego, California, USA). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... 1000ng total RNAs from each sample were used to make library according to the product guide of Truseq stranded mRNA library kit (Illumina, Cat# 20020595). In short ...
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bioRxiv - Neuroscience 2020Quote: ... and 40 from each of the 10 donors) were prepared with the TruSeq Stranded mRNA kit from Illumina (San Diego, CA, USA) and sequenced on a HiSeq 4000 sequencer (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 140 pg of the amplified cDNA was fragmented using Nextera XT DNA sample preparation kit (Illumina FC-131-1096) and amplified with Nextera XT indexes (Illumina FC-131-1001) ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA libraries were prepared using 200 ng of good quality total RNA according to the manufacturer’s instructions for the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA), employing poly-A mRNA enrichment using oligo dT magnetic beads ...
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bioRxiv - Genomics 2020Quote: Total RNA obtained from plasma was desiccated to a volume of 2.5μl for library preparation with the TruSeq Small RNA Library Prep kit (Illumina, Inc., San Diego, CA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ul H2O) at 37◻°C for 30◻min (TruePrep® DNA Library Prep Kit V2 for Illumina, Vazyme, China). After the tagmentation ...
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bioRxiv - Microbiology 2020Quote: ... as previously described.145,146 Deep-sequencing libraries were prepared using the Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on MiSeq (Illumina).
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bioRxiv - Plant Biology 2020Quote: 42 RNA samples from experiment 1 were used for mRNA-Seq library preparation using the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA). Libraries were multiplexed and single-end 100 nt RNA-Seq was performed on a Novaseq 6000 at the GIGA Center (University of Liege ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared separately from each of the three biological replicates of each tissue type using the TruSeqTM RNA Sample Preparation Kits (v2) and sequenced using the Illumina HiSeq 2500 instrument (Illumina Inc., USA) at the Center for Genomic Research and Biocomputing ...
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bioRxiv - Genomics 2020Quote: ... Complementary DNA (cDNA) libraries were constructed for each snail individual using TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Libraries for sequencing on the Illumina platform were prepared to apply the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, USA) with modifications according to Kishony et al ...
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bioRxiv - Plant Biology 2021Quote: ... The pooled library was sequenced on an Illumina MiSeq platform using a 2× 300-bp MiSeq Reagent Nano Kit v2 (Illumina, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... The final purified product was quantified using qPCR according to the qPCR Quantification Proto-col Guide (KAPA Library Quantification kits for Illumina Sequencing platforms) and qualified using the LabChip GX HT DNA High Sensitivity Kit (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... 2.5 µg of confirmed DNA-free total RNA was treated with Ribo-Zero rRNA Removal Kit for Bacteria according to the manufacturer’s instructions (Illumina, San Diego, CA). Following rRNA removal ...
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bioRxiv - Microbiology 2019Quote: ... The rRNA genes were removed from the total RNA using the Ribo-Zero rRNA removal kit (Illumina, Inc., San Diego, CA, USA) and the remaining mRNA was reverse-transcribed ...
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bioRxiv - Molecular Biology 2021Quote: ... All single cell sequencing libraries were generated using 10x Chromium Next GEM v3.1 kits (10x Genomics) and sequencing was performed on a Novaseq6000 system (Illumina, San Diego, California), using an S2 v1.5 100 cycles flow cell (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... and submitted for DNA sequencing using the MiSeq Reagent kit v3 with the 2 × 300 bp paired-end sequencing protocol (Illumina Inc., USA).
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bioRxiv - Microbiology 2019Quote: ... Dual barcoded Illumina libraries were prepared with the Nextera XT DNA kit according to the manufacturer’s protocol (Illumina, San Diego, CA, USA). Library size distribution was determined using a High-Sensitivity DNA Kit run on a Bioanalyzer 2100 instrument (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Metagenomic shotgun libraries were prepared for each sample using the Nextera XT DNA Sample Preparation Kit (Illumina Inc., San Diego, CA, USA) and sequencing was performed on an Illumina NextSeq500 platform with a 2 × 150 bp High Output run ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF10A libraries from three biological replicates of each cell type were prepared using NEBNext® Ultra(tm) RNA Library Prep Kit (Illumina®) and sequenced on HiSeq X by Annoroad Gene Technology Ltd ...
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bioRxiv - Cancer Biology 2021Quote: ... In brief, RNA-seq libraries (non-strand-specific, paired end) were prepared with the NEBNext® Ultra™ RNA Library Prep Kit (Illumina) and were sequenced according to the paired-end 150bp protocol using NovaSeq 6000 ...
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bioRxiv - Cell Biology 2020Quote: ... The remainder of the ATAC-seq library preparation was performed by following manufacturer’s instructions (Nextera DNA Flex Library Prep kit, Illumina, cat. no. 20018704) and the sample quality was validated by BioAnalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2019Quote: RNA-Seq libraries were constructed from 200 ng of total RNA using Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold sample prep kits (Illumina, Carlsbad, CA). Constructed libraries contained RNAs >200 nt (both unpolyadenylated and polyadenylated ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were processed on the Illumina HiSeq 550 sequencing system with the NextSeq 500/550 High Output kit v2.5 (Illumina, Cat. No. 20024906). The generated fastq files were loaded into the DolphinNext platform (https://dolphinnext.umassmed.edu/ ...
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bioRxiv - Physiology 2022Quote: ... high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial 16S rRNA V4 region was amplified during library preparation via Illumina’s Nextera XT Index Kit v2 (Illumina, Inc.; San Diego, CA) (primers ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of purified RNA in each replicate and condition was used for strand-specific RNA-seq library preparation using the Illumina Stranded mRNA Prep kit (Illumina, Cat. 20040534) and IDT Illumina RNA UD Indexes Set B (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... were pooled and RNA extraction/purification was performed for the preparation of sequencing libraries using the Illumina Stranded mRNA Prep kit (Illumina, Cat. 20040534) and IDT Illumina RNA UD Indexes Set B (Illumina ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared with 1 μg of total RNA for each sample using a TruSeq Stranded mRNA Sample Prep Kit (Illumina, CA, USA). Paired-end sequencing (151 bp ...
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bioRxiv - Cancer Biology 2022Quote: We prepared samples for RNA-seq using the TruSeq Stranded Total RNA Sample Preparation kit with RiboZero Gold (Illumina, San Diego, CA). We performed RNA sequencing for each sample to ∼ 80 million paired-end 150 bp reads on an Illumina NextSeq 500 (Princess Margaret Genomics Centre ...
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bioRxiv - Cell Biology 2022Quote: ... Next generation sequencing library preparations were constructed according to the manufacturer’s protocol (NEBNext® Ultra™ RNA Library Prep Kit for Illumina®). The sequences were processed and analyzed by GENEWIZ (Suzhou ...
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bioRxiv - Cell Biology 2022Quote: ... and stranded total RNA-seq libraries were prepared using the Illumina TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA, USA) implemented on the liquid handling robot Beckman FXP2 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA libraries were prepared using approximately 100 ng of total RNA according to the manufacturer’s instructions for the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA), employing poly-A mRNA enrichment using oligo dT magnetic beads ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were diluted and 150 pg input cDNA was used with the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) to generate indexed libraries ...
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bioRxiv - Immunology 2020Quote: RNA libraries were prepared using 100 ng of good quality total RNA according to the manufacturer’s instructions for the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA) employing poly-A mRNA enrichment using oligo dT magnetic beads ...