Labshake search
Citations for Illumina :
8901 - 8950 of 9681 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Pooled cDNA libraries were sequenced paired end using an Illumina NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles Illumina, San Diego, US) on an Illumina NovaSeq 6000 instrument ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 300 ng of RNA was used for library prep (NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina) following standard kit procedure ...
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bioRxiv - Cell Biology 2023Quote: RNA libraries were prepared using 200 ng of total RNA in 20 µl according to the manufacturer’s instructions for the Illumina Stranded mRNA Ligation Sample Prep Kit (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined using an Agilent Bioanalyzer DNA 1000 chip and Qubit fluorometry (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Whole-genome shotgun sequencing was carried out on an Illumina MiSeq platform using Nextera DNA Flex kits (Illumina, San Diego, CA, USA) with a 150-bp paired-end sequencing kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... A library was prepared with 100 ng of total RNA for each sample using the TruSeq RNA Access library prep kit (Illumina, CA, USA). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing was done using Illumina NextSeq 500 sequencer using the Nextseq High Output V2 150 sequencing kit (Illumina, 150 bp, single reads). RNAseq bulk sequencing data (from MARS-seq ...
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bioRxiv - Physiology 2023Quote: ... high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing ...
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bioRxiv - Immunology 2023Quote: ... The 1.2 pM library was sequenced on the NextSeq using the NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) (Illumina, cat. no: 20024905) at UCL genomics.
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries for transcriptome analysis (RNA-Seq) were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The NGS library was prepared with the Nextera XT DNA Library prep kit following manufacturer’s instructions with changes (Illumina #FC-131-1096). Initially ...
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bioRxiv - Microbiology 2023Quote: ... Resultant genomic DNA was treated with RNase A and prepared for sequencing using the Nextera XT kit (Illumina Corp., San Diego, CA). Libraries were uniquely dual-indexed and pooled ...
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bioRxiv - Genomics 2023Quote: ... RNA libraries were created and individually barcoded from 2.7 µg of template total RNA utilizing the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) as described in the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the KAPA RNA HyperPrep Kit and sequenced on an Illumina HiSeq® 4000 sequencer (Illumina, San Diego, CA) at a minimum of 25 million paired end reads (75bp ...
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bioRxiv - Microbiology 2023Quote: ... The Seqwell plexWell LP384 Library Preparation Kit and Native Barcoding Kit 24 V14 (SQK-NBD112.24) were used for barcode-indexed whole genome sequencing with Illumina NovaSeq system (Illumina San Diego, USA) and Oxford Nanopore MinIoN Mk1C device (Oxford Nanopore Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Complementary DNA libraries from total RNA samples were prepared with Illumina TruSeq RNA preparation kits according to the manufacturer’s instructions (Illumina, San Diego, CA). RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Santa Clara ...
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were sequenced with 54 paired-end reads on a NovaSeq-6000 using a Reagent Kit v1.5 (Illumina Inc., San Diego). For CD45+ RNA-seq and the cell line RNA-seq ...
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bioRxiv - Microbiology 2023Quote: ... A second 4 nM pool was generated and sequenced on the Illumina NovaSeq 6000 with the NovaSeq 6000 S4 Reagent v1.5 (300 cycles) kit (20028312, Illumina, San Diego, CA) at the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute (Boston ...
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bioRxiv - Immunology 2023Quote: ... 150 picograms of cDNA was used to make sequencing libraries by Nextera XT DNA Sample Preparation Kit (Illumina catalog#: FC-131-1024). Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific catalog# ...
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bioRxiv - Molecular Biology 2023Quote: We constructed an assay for transposase-accessible chromatin (ATAC) using a Tn5-Nextera DNA library prep kit (Illumina, San Diego, CA, USA), following the manufacturer’s protocol (48) ...
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bioRxiv - Genetics 2024Quote: Libraries were sequenced at between 40,000 and 50,000 fragment reads per cell following Illumina’s standard protocol using the Illumina cBot and HiSeq 3000/4000 PE Cluster Kit (Illumina, San Diego, CA). The flow cells were sequenced as 100 × 2 paired end reads on an Illumina HiSeq 4000 HD using HiSeq 3000 / 4000 sequencing kit and HCS v3.4.0.38 collection software ...
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bioRxiv - Molecular Biology 2024Quote: ... Small RNA libraries were generated using NEXTFlex Small RNA Library Prep Kit v3 (Cat #NOVA-5132–06) and sequenced on the NextSeq 500 system (Illumina, CA, USA) by the Genomic Unit at Genyo ...
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bioRxiv - Microbiology 2024Quote: ... The library preparation and ribosome depletion were carried out using the Illumina Stranded Total RNA prep Ligation with Ribo-Zero Plus kit according to the manufacturer’s protocol (Illumina, San Diego, USA). The libraries were sequenced on the Illumina NextSeq1000 platform (1×100 bp ...
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bioRxiv - Microbiology 2024Quote: ... and 1ng of the cDNA was employed as a template for the construction of the sequencing library using the NexteraXT kit from (Illumina, Cambridge, UK). Subsequently ...
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bioRxiv - Microbiology 2024Quote: Genomic libraries of isolates from the CV region outbreak were prepared using the Illumina DNA Prep kit (Illumina, San Diego, CA, USA) and sequenced on a Nextseq instrument generating 2x150bp paired-end sequencing reads following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit v2 according to the manufacturer’s protocol (Illumina, San Diego, CA, USA). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Next generation sequencing library preparations were constructed following the manufacturer’s protocol (NEBNext® Ultra™ DNA Library Prep Kit for Illumina®). For each sample ...
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bioRxiv - Microbiology 2024Quote: Indexed sequencing libraries were generated from 1 ng genomic DNA using Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the protocol of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... RNA libraries were generated from 200 ng RNA using the Illumina total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina, United States). In brief ...
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bioRxiv - Genomics 2024Quote: ... Libraries with 5% PhiX spike-in were sequenced on an Illumina NextSeq 2000 sequencer with P3 200 cycles kits (Illumina cat. #20040560) as paired-end ...
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STAT5B SH2 variants disrupt mammary enhancers and the stability of genetic programs during pregnancybioRxiv - Genetics 2024Quote: ... Libraries for sequencing were prepared according to the manufacturer’s instructions with TruSeq Stranded Total RNA Library Prep Kit with Ribo- Zero Gold (Illumina, RS-122-2301) and paired-end sequencing was done with a NovaSeq 6000 instrument (Illumina).
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bioRxiv - Genomics 2024Quote: ... Total RNA-seq libraries were generated from 1 μg of DNase-treated RNA using the TruSeq Stranded total RNA library preparation kit (Illumina, Cat# 15031048) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA extracted from these fruits was pooled to create a single sample for the construction of cDNA libraries using the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., USA) (Illumina ...
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bioRxiv - Genomics 2024Quote: ... RNA extracted from these fruits was pooled to create a single sample for the construction of cDNA libraries using the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., USA) (Illumina, Inc., USA). The Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Systems Biology 2024Quote: ... Sequencing library preparation was conducted per the guidelines provided in the MiSeq™ Reagent Kit Preparation Guide (Illumina, San Diego, CA, USA) as outlined by (Caporaso et al. ...
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bioRxiv - Plant Biology 2024Quote: ... RNA libraries for sequencing were prepared using TruSeq Stranded mRNA sample prep kit with 96 dual indexes (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions with the following changes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... resuspended in 25 μL of 2x TD buffer and 2.5 μl of Tn5 transposase from the Nextera DNA Library Prep kit (Illumina, San Diego, CA), and incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... RiboZero: rRNA was removed from 10 ng of total RNA using Ribo-Zero(TM) rRNA Removal Kit (Illumina, San Diego, CA, USA). Stranded cDNA libraries were generated using the Illumina Truseq Stranded mRNA Library Prep kit ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was used to create each sequencing library using a Truseq RNA Sample Preparation Kit (Illumina, RS-122-2001). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... ATAC-seq libraries for murine endothelial cells were processed as previously described (Buenrostro et al., 2015) and libraries were generated using the Nextera DNA Sample Preparation Kit (Illumina, FC-121-1030). The quality of purified DNA libraries was checked by Agilent High Sensitivity DNA kit (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng of total RNA from each sample was used for the RNA-seq library preparation using the TruSeq stranded mRNA kit (Illumina, San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were prepared from HepG2 cell samples using 200–500 ng of total RNA following the manufacturer’s instructions for the TruSeq RNA kit (Illumina, San Diego, CA, USA). With this kit ...
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bioRxiv - Cell Biology 2019Quote: ... The rRNA depleted samples were fragmented and sequencing libraries prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Fragments were then indexed with 10 nucleotide barcode sequences using Nextera XT Index Kit v2 Set D primers (Illumina, San Diego, California, USA). Indexing PCR reactions were run in triplicate with a volume of 25 μL using 12.5 μL NEB Next High-Fidelity Master Mix ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared starting from 1000 ng of total RNA using the RNA Sample Prep Kit v2 (Illumina Inc, San Diego, CA, USA) including a poly-A selection step following the manufacturer’s instructions and sequenced as 2 x 100 nt paired-end reads using an Illumina HiSeq 2500 ...
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bioRxiv - Microbiology 2021Quote: ... Jude Children’s Research Hospital Hartwell Center with DNA libraries prepared using Nextera XT DNA-Seq library prep kits (Illumina, cat#FC-131-1024) with 96 dual-index bar codes and sequenced on an Illumina MiSeq personal genome sequencer ...
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bioRxiv - Genomics 2019Quote: ... We prepared cDNA libraries for the three biological replicates per genotype using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA), and performed single-end sequencing using Illumina HiSeq 2000 at the Penn State Genomics Core Facility to obtain 100 bp reads at an average coverage of 36.0 million aligned reads/sample ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries were generated using Illumina SureCell WTA 3’ Library Prep Kit for the ddSEQ System (6 cartridge version, cat.no. 20014280, Illumina, San Diego, CA, USA). Libraries were assessed for quality ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was isolated by magnetic bead and converted into RNA libraries using the Illumina TruSeq RNA Library preparation kit v2 according to the manufacturer’s instructions (Illumina, San Diego, CA, USA). Libraries were sequenced on a HiSeq 2100 ...
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bioRxiv - Microbiology 2021Quote: ... The library was loaded at 6 pM and sequenced using the Miseq Reagent Kit v3 (600-cycle) (Illumina; catalog no., MS-102-3003).
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bioRxiv - Evolutionary Biology 2021Quote: ... Paired-end libraries were constructed from 100 ng of the total RNAs using TruSeq RNA Library Prep kits v2 (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...