Labshake search
Citations for Illumina :
8301 - 8350 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and paired end read sequencing (2 x150bp) was performed using MiSeq platform and reagent kit v2 (Illumina, San Diego, CA, USA). For sequences obtained by HTS a pre-alignment quality check was performed using the FASTQC program v0.11.8 and the Trim Galore script (52 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg of high-quality total RNA (RIN > 9.9) was processed with Truseq Stranded Total RNA Library Prep Kit (Illumina; Cat#: 20020596) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... cDNA was subsequently tagmented and amplified for 12 cycles by using a Nextera XT DNA Library Preparation Kit (Illumina FC-131). Sequencing libraries were analyzed with Qubit and Agilent Bioanalyzer ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... cDNA libraries were prepared using 1 μg of total RNA with the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, USA). The cDNA library was qualified on the Agilent Technologies 2100 Bioanalyzer to check the library quality and fragment size of the sample ...
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bioRxiv - Plant Biology 2020Quote: ... The gDNA was isolated by using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Sequencing was performed on an Illumina MiSeq platform at the University of Manchester genomics facility using shotgun 2×250 paired-end sequencing methodology (Nextera DNA Preparation Kit, Illumina, USA). The sample used 0.33 of a flow cell and primer design was optimized for use with Platinum Taq DNA polymerase (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... Short read RNA-Seq libraries were also prepared for all nine tissue using TruSeq stranded mRNA LT kits and supplied protocol (Illumina, CA), and sequenced on a NextSeq500 platform using v2 sequencing chemistry to generate 2 x 75 bp paired-end reads.
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bioRxiv - Plant Biology 2021Quote: ... Two 150-bp single-end sequencing libraries were prepared using the NextSeq 500/550 High Output Kit (Illumina, San Diego, USA) and sequenced on a NextSeq 500 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Metatranscriptomic sequencing libraries were also constructed by Genome Quebec using an Illumina TruSeq RNA Library Preparation kit (Illumina, Inc., CA, US) after rRNA depletion ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: ... The strand specific RNAseq library was prepared using the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, Palo Alto, CA, USA) at the Oxford Genomics Center ...
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bioRxiv - Microbiology 2021Quote: ... A total amount of 100 ng total RNA per sample was used to prepare for the rRNA-depleted cDNA library by Stranded Total RNA Prep Ligation with Ribo-Zero Plus Kit (Illumina, USA). The final library size and quality was evaluated using an Agilent High Sensitivity DNA Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... DNase-treated RNA extracts were used for library preparation using the TruSeq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Transposase Enabled (LITE) Illumina compatible libraries were constructed using a modified protocol based on the Illumina Nextera kit (Illumina, California USA). A total of 1ng of DNA was combined with 0.9 μl of Nextera reaction buffer and 0.1 μl Nextera enzyme in a reaction volume of 5 μl and incubated for 10 minutes at 55°C ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... A total of 100 ng of RNA input was then used for cDNA library preparation using the TruSeq® Stranded mRNA NeoPrep kit (Illumina), according to manufacturer instructions ...
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bioRxiv - Pathology 2021Quote: Samples were subjected to mRNA enrichment through eukaryote and prokaryote rRNA removal using the Ribo-Zero rRNA Removal Epidemiology Kit (Illumina®), following the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2020Quote: ... DNA was isolated and used to prepare ChIP-seq libraries with the TruSeq ChIP Library Preparation Kit (Illumina, IP-202-1012). Sequencing was performed on an Illumina NextSeq500 in Georgia Genomics and Bioinformatics Core (GGBC ...
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bioRxiv - Microbiology 2021Quote: RNA samples from three biological replicates of BeWo cells infected with WT or xrRNA2’ mutant ZIKVMR766 or mock-infected were subjected to poly(A) RNA enrichment and library preparation using TruSeq 2 Library Preparation Kit (Illumina, USA). Libraries were sequenced on an Illumina NextSeq 500 instrument using NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared from the genomic DNA using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA, USA) and sequenced using Illumina MiSeq sequencing platforms (2□×□250-bp paired-end reads) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq library preparation was performed with 1ug of total RNA per sample using the TruSeq Stranded mRNA Library prep kit (Illumina, 20020595) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were prepared using the Nextera (summer and fall samples) or TruSeq (spring samples and all metatranscriptomic samples) preparation kits (Illumina Inc.), and sequenced on a 2 × 150 bp paired-end HiSeq 2000 sequencing system ...
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bioRxiv - Molecular Biology 2021Quote: ... Approximately 25 μg of total RNA was used to deplete ribosomal RNA according to the Epicentre Ribo-Zero Gold Kit (MRZG126, Illumina, USA) manual ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2000 copies/cell yeast SUMO to each sample then proceed with rRNA removal Ribo Zero Gold kit (Illumina cat # MRZ11124C) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were cleaned using Sample Purification Beads from the TruSeq Methyl Capture EPIC LT Library Prep Kit (Illumina, #FC-151-1002) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 µg of total RNA was used for library preparation using the TruSeq Stranded mRNA kit (Illumina, cat# RS-122-2103) after rRNA depletion using Ribo-Zero plus (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... RNA libraries for sequencing were generated in triplicate by using the same amount of RNA for each sample according to the Illumina TruSeq Stranded Total RNA kit with an initial ribosomal depletion step using Ribo Zero Gold (Illumina, CA). The libraries were quantified by RT-qPCR and sequenced in paired-end mode (2x75 bp ...
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bioRxiv - Microbiology 2021Quote: ... Illumina shotgun libraries were prepared using the Nextera DNA Sample Preparation kit as recommended by the manufacturer (Illumina, San Diego, USA). To assess quality and size of the libraries ...
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Altered TGFB1 regulated pathways promote accelerated tendon healing in the superhealer MRL/MpJ mousebioRxiv - Cell Biology 2021Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... and strand-specific RNA-seq libraries were constructed using the TruSeq Stranded Total RNA preparation kits (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions before deep sequencing.
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bioRxiv - Genomics 2021Quote: ... the libraries were sequenced as 150-bp single-end reads using the High Output v2 Kit (Illumina, San Diego, CA, USA) for the NextSeq 500 platform (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and ∼ 200 bp insertion size libraries were constructed using the Illumina whole transcriptome analysis kit following the manufacturer’s standard protocol (Illumina, HiSeq system). All libraries were then sequenced on the Illumina HiSeq 2000 at the sequencing core facility at Max Planck Institute for Molecular Genetics (Berlin) ...
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bioRxiv - Cancer Biology 2020Quote: ... Small fraction sequencing libraries were prepared using the Illumina TruSeq Small RNA Library Prep Kit (Illumina, catalog no. RS-200-0012), and poly-A sequencing libraries (HE2.1 and TIVE cells ...
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bioRxiv - Cancer Biology 2021Quote: RNA isolation of three independent biological replicates was performed as described above and RNA-seq libraries prepared using the TruSeq mRNA Library preparation kit (Illumina #20020595). RNA sequencing was performed on an Illumina HiSeq2500 machine (50 bp single-end reads) ...
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bioRxiv - Immunology 2022Quote: ... a multiplexed amplicon-based whole-viral-genome approach using the NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®) was employed (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Short reads were sequenced by the PennCHOP microbiome program sequencing core using the Nextera XT library preparation kit (Illumina, CA, USA) and the Illumina HiSeq 2500 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... ribosomal RNAs of both insect and bacterial origins were removed from the total RNA samples using Ribo-Zero Gold rRNA Removal Kit (Epidemiology) (Illumina, USA). The rRNA-depleted RNAs were converted to paired end libraries using Sure Select Strand Specific RNA Kit (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were sent to BGI Hong Kong for yeast rRNA removal (Ribo-Zero Gold rRNA Removal kit by Illumina (now discontinued)) ...
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bioRxiv - Microbiology 2022Quote: ... Library preparation for Illumina metagenome sequencing was performed using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA). Metagenomes were obtained for 69 samples across two paired-end NextSeq (132–170 bp ...
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bioRxiv - Microbiology 2021Quote: ... Library preparation of amplified SARS-CoV-2 DNA for sequencing was performed using a Nextera XT library prep kit (Illumina Inc.) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Library preparation of amplified SARS-CoV-2 DNA for sequencing was performed using a Nextera XT library prep kit (Illumina Inc.) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and the V(D)J library was sequenced with NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) (Illumina, cat. 20024905).
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were pelleted (500xg, 10 min, 4 °C) and tagmented with the Nextera DNA Library Prep Kit (Illumina, FC-121-1030) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were normalized to 2 nM and sequenced using the MiSeq Reagent Kit v3 (600-cycle) (Illumina, San Diego, CA, USA) following the 2 x 276 bp paired-end read protocol.
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bioRxiv - Genomics 2022Quote: ‘Amplify cDNA’ COVIDSEQ™ PCR Master Mix 1 and 2 were formulated using either the V3 primer pools (provided in the COVIDSEQ™ Library Preparation kit) or an aliquot of the V4 primer pools manufactured by Illumina, Inc for direct comparison of all specimens.
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bioRxiv - Microbiology 2022Quote: Sequencing library was prepared with TruSeq Stranded mRNA Sample Prep Kit and sequenced on NovaSeq 6000 (Illumina, San Diego, CA, USA), yielding more than 6G bases of sequences for each sample ...
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bioRxiv - Genomics 2022Quote: ... Illumina libraries with 300-400 bp inserts were generated using the Illumina Nextera XT kit according to the manufacturer’s protocol (Illumina, CA, USA). Samples were multiplexed with the Illumina Nextera XT Index kit containing 96 indices ...
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bioRxiv - Genomics 2022Quote: ... RNAseq libraries were constructed from all reference tissues at the University of Montana using an Illumina TruSeq Stranded mRNA library kit (Illumina, 20020595), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Successfully amplified samples were prepared for paired end short-read sequencing using the Nextera XT Library Prep kit (Illumina, California, USA), individual libraries being pooled in equimolar amounts at 4nM with up to 12 per pool ...
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bioRxiv - Neuroscience 2022Quote: ... RNA samples were converted into cDNA libraries using the Illumina TruSeq Stranded Total RNA sample preparation kit (Illumina # RS-122-2303). Briefly ...
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bioRxiv - Genomics 2022Quote: ... A 3-kb mate-pair library was created for each butterfly species using the Nextera Mate-pair Library Gel-plus kit (Illumina, Inc.). The libraries were then sequenced on a HiSeq 2000 (2×100 bp reads ...
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bioRxiv - Microbiology 2022Quote: ... and sequenced on an Illumina NovaSeq using single-end 1×100 reads with the Illumina NovaSeq SP reagent kit (Illumina, 20027464). A total of 122,190,150 raw sequence reads (mean of 4,213,453 per sample (n = 29 ...