Labshake search
Citations for Illumina :
8101 - 8150 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were processed using the Chromium Next GEM Single Cell 3’ Reagent Kits (10x genomics) and sequenced on a NovaSeq 6000 (Illumina) at the Harvard Medical School Biopolymers Core Facility as 28 (read 1 ...
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bioRxiv - Microbiology 2023Quote: Dual-indexed paired-end libraries for whole genome sequencing were prepared with the Nextera DNA Flex Library Prep Kit from Illumina. Individual libraries were barcoded with Nextera DNA CD Indexes (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... 3-5 µg of each sample was treated with Ribozero® rRNA Removal Kit according to the manufacturer’s instructions (Illumina, USA ...
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bioRxiv - Neuroscience 2023Quote: ... 500 ng of RNA was used as an input and libraries were prepared using the TruSeq Stranded mRNA library preparation kit from Illumina. The samples were sequenced on the NovaSeq 600 platform on the NovaSeq S4 300 cycle flowcell to generate 150 bp paired-end reads ...
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bioRxiv - Cancer Biology 2023Quote: ... Final DNA libraries sequencing was performed in Illumina NovaSeq 6000 platform using the NovaSeq 6000 S1 Reagent Kit 300 cycles (2 x 150 paired-end reads) (Illumina). Sequencing data were analysed with Illumina DRAGEN Bio-IT Platform v4.0 using proprietary pipelines for variant calling ...
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bioRxiv - Microbiology 2023Quote: ... RNA extracts from the microneutralisation and TCID50 experiments were used as input into the RNA Prep with Enrichment kit (Illumina Inc ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of PCR product for each sample were normalized in 30 µl volume for NGS library construction using Illumina DNA Prep (M) Tagmentation kit (Illumina). IDT for Illumina Nextera DNA Unique Dual Indexes were used for pooling multiple samples together ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting libraries were amplified using the NEBNext® Ultra™ DNA library prep kit for Illumina® using NEBNext® multiplex oligos for Illumina® and sequenced on a NextSeq 500 with onboard clustering using a 75-cycle high output v2.5 kit (Illumina) to generate paired-end 41-bp-long reads ...
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bioRxiv - Developmental Biology 2023Quote: ... Illumina sequencing libraries were prepared from the amplified cDNA from each sample group using the Nextra DNA library prep kit (Illumina) and then sequenced on Illumina HiSeq 2500 Rapid flowcells (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... One ng cDNA was used for library preparation using Nextera XT DNA Library Preparation Kit (FC-131-1024 & FC-131- 1096, Illumina). The yield and quality of the amplified libraries were analyzed using Qubit by Thermo Fisher and the Agilent Tapestation System ...
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bioRxiv - Systems Biology 2023Quote: ... the lineage barcode library was sequenced with a NextSeq 500 with a Mid Output Kit v2.5 (150 cycles, Illumina, 20024904) by paired-end sequencing using 8 cycles on each index ...
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bioRxiv - Bioengineering 2023Quote: ... Library was sequenced on an Illumina MiSeq instrument using Illumina MiSeq Reagent Kit v2 Micro (300-cycles) with 50% PhiX spike-in (Illumina). After demultiplexing ...
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bioRxiv - Bioengineering 2023Quote: ... For 4-week and 8-week organoids cDNA library was prepared by using the whole transcriptome Illumina TruSeq Stranded Total RNA Library Prep Kit Gold (Illumina), followed by evaluation on an Agilent 2100 Bioanalyzer by using the DNA 1000 kit ...
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bioRxiv - Bioengineering 2023Quote: ... primers were designed containing a universal linker sequence allowing amplicons for incorporation indexes and sequencing primers by Nextera XT Index kit (ILLUMINA); and 16S rRNA gene universal primers (Klindwoth2013) ...
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bioRxiv - Cancer Biology 2023Quote: ... a total RNA input of 200 ng was used for library preparation using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Kit (Illumina) and IDT for Illumina RNA UD Indexes Set A ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng DNase treated RNA was used to generate ribo-minus treated total RNA stranded libraries using Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina) according to manufactures’ instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 250ng of total RNA from mature pollen and 1000 nanograms of total RNA from inflorescences or leaf tissues were used for RNA-seq library preparation with TruSeq Stranded mRNA kit (Illumina). The final library was sequenced on Illumina NovaSeq 6000 or HiSeq 4000 instruments.
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bioRxiv - Neuroscience 2023Quote: ... The resulting clones were screened by multiplexed amplicon sequencing on an Illumina Mi-Seq platform with the MiSeq Reagent Kit V2 (Illumina). Clones identified with CRISPREsso [22] to be unchanged or to be carrying indels ...
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bioRxiv - Cancer Biology 2023Quote: ... in single-end mode using the NextSeq 500/550 High Output Kit (75 Cycles) (Illumina, cat. no. FC-404-2005) except for scCUTseq libraries from brain and skeletal muscle ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Sequencing Facility at NCI-Leidos using the TruSeq Stranded mRNA Kit (Illumina, San Diego, CA) and sequenced in a paired-end manner on NextSeq (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: Dual indexed FFPE libraries were constructed with a 500 ng input of total RNA utilizing the TruSeq Stranded Total RNA Library Construction riboGold kit (Illumina). Two pools of five samples each were pooled at an equimolar ratio yielding ∼900ng (Pool 1 ...
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bioRxiv - Microbiology 2023Quote: ... and sequenced as 2 x 150 bp reads on the MiSeq instrument using the MiSeq Reagent Micro kit v2 (Illumina). Raw image files were converted to fastq files using bcl2fastq (v2.20.0.422 ...
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bioRxiv - Plant Biology 2023Quote: ... Library preparation and sequencing were carried out according to the instructions with the TruSeq RNA Library Prep Kit v2 (Illumina) and NextSeq 500/550 High Output Kit (Illumina) ...
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bioRxiv - Physiology 2023Quote: ... Libraries were prepared using the Takara SMART-Seq v4 Ultra low input RNA kit and analyzed on a NovaSeq platform (Illumina) using paired-end (PE100/150 ...
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bioRxiv - Developmental Biology 2023Quote: ... The clustering of the index-coded samples was performed by Novogene on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina). Paired-end clean reads were mapped to the reference genome using HISAT2 software and for samples with biological replicates ...
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bioRxiv - Genetics 2023Quote: ... Sequencing was performed on an Illumina NovaSeq 6000 using PE 150 bp High Output kit (Illumina, San Diego, California, USA).
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bioRxiv - Developmental Biology 2023Quote: Oocyte and granulosa cell samples were processed for full-length cytoplasmic RNA amplification using a slightly modified SMART-seq2 protocol.108 Libraries for next generation sequencing were prepared using Nextera XT DNA library Prep Kit (Illumina). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were sequenced on the Illumina NextSeq 500 over two NextSeq 500 High Output v2.5 150 cycle kits (Illumina; 20024907) to generate > 25,000 raw reads per cell for each sample ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We prepared short-insert (350 bp) paired-end (PE) libraries from 100 ng of gDNA using a TruSeq Nano DNA LT Library Preparation Kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then diluted to 150 pM in elution buffer supplied in the library prep kit and loaded to the iSeq100 flow cell for sequencing on iSeq100 (Illumina). After the NGS run ...
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bioRxiv - Cancer Biology 2023Quote: ... The ChIPed and the Input DNA were used to prepare libraries by Kappa Hyperprep kit and sequenced in Nextseq (Illumina).
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bioRxiv - Immunology 2023Quote: ... and DNA amplicon products where generated using Ig-specific variable and constant gene primers.87 Samples were indexed (Nextera Index Kit, Illumina), purified ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were processed according to Chromium 3’ Gene Expression V3 Kit (10X Genomics) using the manufacturer’s guidelines followed by sequencing on an S1 Novaseq chip (Illumina Inc.) at the Genomics Core of Icahn School of Medicine at Mount Sinai.
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bioRxiv - Cancer Biology 2023Quote: The RNA sequencing was performed by the Yale Center for Genome Analysis (YCGA) after the removal of rRNA from RNA samples using the Ribo-Zero rRNA Removal Kit from Illumina. The RNA libraries were created using the TrueSeq Small RNA Library preparation kit from Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... was isolated using PureBind Blood Genomic DNA Isolation Kit (Ocean Nanotech), quantified, library preparation was performed, and quality was assessed (LAB-SOP-018, GeneGoCell) then sequenced (NextSeq2000, Illumina). Raw sequence reads were demultiplexed into sample-specific fastq files (bcl2fastq program v2.20.0.422 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA libraries were prepared for sequencing by the Cancer Genomics Shared Resource (Wake Forest University School of Medicine, Winston-Salem, NC) using the TruSeq-stranded total RNA kit (Illumina), which includes a ribosomal depletion step ...
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bioRxiv - Molecular Biology 2023Quote: ... 11200g for five minutes prior to dividing the lysate for either nuclease foot-printing with RNaseI for generation of the ribosome profiling libraries or total RNA extraction followed by removal of rRNA with RiboZero (plant leaf kit, Illumina) for the corresponding RNA-Seq library.
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bioRxiv - Neuroscience 2023Quote: ... The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... after which onboard clonal clustering of the patterned flow cell was performed using a NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycle) (Illumina 20028318). A 2x101 cycle sequencing profile with dual index reads was completed using the following sequence profile ...
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bioRxiv - Neuroscience 2023Quote: ... Eighteen pM libraries were clustered in a high-output flow cell using HiSeq Cluster Kit v4 on a cBot (Illumina). After cluster generation ...
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bioRxiv - Physiology 2023Quote: ... followed by onboard clonal clustering of the patterned flow cell using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycle) (Illumina 20028318). A 2x101 cycle sequencing profile with dual index reads is completed using the following sequence profile ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA library was then fragmented and indexed using the Nextera DNA Library Preparation kit (Illumina, San Diego, CA, USA). The library quality was checked on a BioAnalyzer high-sensitivity chip and concentration was quantified by Qubit assays (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Pooled libraries were sequenced on the Illumina NextSeq 500 platform using the High Output Kit v2.5 for 150 Cycles (Illumina, 20024907).
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bioRxiv - Molecular Biology 2023Quote: ... Purified multiplex libraries were diluted to 9 nM concentration (calculated based on Qubit dsDNA HS Assay Kit) and sequenced on a NovaSeq 6000 instrument (Illumina) from IntegraGen SA ...
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bioRxiv - Genetics 2023Quote: ... libraries were sequenced as 150-bp single-end reads using the High Output v2 Kit (Illumina, San Diego, CA, USA) in a NextSeq 500 platform (Illumina ...
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bioRxiv - Genomics 2023Quote: 1-2 µg of total RNA was depleted of rRNA and globin mRNA using the Globin-Zero Gold rRNA Removal Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The libraries were sequenced in a 250-bp paired-end run using the MiSeq Reagent Kit v2 (Illumina; 500 cycles).
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the TruSeq® Stranded Total RNA LT - Ribo-Zero Gold kit (Illumina RS-122-2301/2) and sequenced on a NextSeq device.
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bioRxiv - Immunology 2023Quote: ... The single-end sequencing was performed on Illumina NextSeq 500 using NextSeq 500/550 High Output Kit v2.5 (75 cycles) (Illumina #20024906) with final reads having length of 76bp ...
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bioRxiv - Genomics 2023Quote: ... Three RNA extractions passing QC (RIN > 9) were pooled in equimolar concentrations and a single transcriptomic library was constructed using Truseq mRNA kit (Illumina) and sequenced on a Illumina Novaseq 6000 sequencer (200M reads ...