Labshake search
Citations for Illumina :
8151 - 8200 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Libraries were sequenced on the Illumina NextSeq500 platform with paired ends for 2 x 150-base pair reads using a Mid-output cartridge Kit v2.5 (Illumina, 20024905). bcl2fastq software was used to demultiplex and compress bcl files into fastq (gz ...
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bioRxiv - Microbiology 2023Quote: ... M9-glycerol or at distinct phases of infection) was subjected to tagmentation using the Nextera DNA Library Prep Kit (Illumina). The DNA was mixed with 10 μL of 2x TD Buffer and 5 μL of Tagment DNA Enzyme TDE1 (diluted 1/50 in 0.5x TE buffer and 50 % (v/v ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated by directly lysing the cells with RLT buffer and using RNeasy Mini kits and used to prepare TruSeq Stranded mRNA (Illumina) sequencing libraries ...
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bioRxiv - Microbiology 2023Quote: ... paired-end cDNA libraries were prepared from cell or EV total RNA by polyA selection using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Whole-genome sequencing (WGS) was performed using the Illumina NovaSeq 6000 with the S4 reagent kit (300 cycles) (Illumina, USA) with 2 × 150 bp paired-end reads ...
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bioRxiv - Genomics 2023Quote: ... we prepared stranded cDNA libraries for 909 samples from 695 sepsis patients using NEB Ultra II LIbrary Prep kits (Illumina) with NEBNext Poly(A ...
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bioRxiv - Genomics 2023Quote: ... 1 ng of the purified fragment was then used to prepare sequencing libraries with the Nextera XT DNA Library Preparation Kit (Illumina, Cat # FC-131-2002 or Cat # FC-131-1024 ...
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bioRxiv - Genomics 2023Quote: ... The sequencing libraries were generated on the streptavidin C1 beads using Illumina Tagment DNA Enzyme and Buffer kit (Illumina, 20034198) and run on the Illumina Novaseq S4 flowcell.
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bioRxiv - Molecular Biology 2023Quote: ... Purified libraries were controlled by capillary electrophoresis (LabChip - Perkin) and quantitated by qPCR (KAPA Library Quantification Kits Illumina Platforms - Roche). Pooled libraries (12 pM ...
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bioRxiv - Developmental Biology 2023Quote: ... Barcoded cDNA libraries were multiplexed onto a TruSeq paired-end flow cell and sequenced (100-bp paired-end reads) with a TruSeq 200-cycle SBS kit (Illumina). Samples were run on an Illumina NovaSeq 6000 sequencer at the KUMC Genome Sequencing Facility ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA samples that passed quality control were used to generate sequencing libraries using the Nextera XT DNA Library Preparation Kit (Illumina), following the user guide ...
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bioRxiv - Cancer Biology 2023Quote: Bead-purified cDNA pools were used for tagmentation-based library preparation with a Nextera XT DNA Library Preparation Kit (Illumina) using a custom PCR amplification strategy to generate 3’ biased libraries containing oligodT cell barcodes as previously published,60 with some modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Sequencing Facility at NCI-Leidos using the TruSeq Stranded mRNA Kit (Illumina, San Diego, CA) and sequenced paired-end on NextSeq (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... The 100-cycle paired end sequencing run was performed using a NovaSeq 6000 S1 Reagent Kit v1.5 200 cycle (Illumina 20028318). Sequence data was converted to fastq files ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 3) DNA Sequencing and Genomics Laboratory (BIDGEN): Libraries were prepared using the Nextera™ DNA Flex Library Preparation Kit (Illumina) and sequenced on a NovaSeq6000 to generate 2 x 150 bp reads ...
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bioRxiv - Evolutionary Biology 2023Quote: Complementary DNA (cDNA) libraries were prepared using the Illumina TruSeq v2 mRNA sample prep kit (Illumina, San Diego, CA, USA), following a standard protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA samples were sequenced using Truseq Stranded Total RNA Library Prep Kit and Illumina HiSeq4000 PE100 (Illumina, San Diego, CA) by the Genome Quebec Innovation Centre ...
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bioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq RNA Library Prep Kit v2 (Illumina) with poly(A ...
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bioRxiv - Bioengineering 2023Quote: ... pair-end single index sequencing parameters were adjusted to 101/6/0/86 (as opposed to 74/6/0/86) to effectively utilize the capabilities of the NovaSeq 200 cycle reagent kit (Illumina). To demultiplex each sample and to generate the matrix of transcript counts in each cell ...
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bioRxiv - Neuroscience 2023Quote: ... Preparation of the RNA sample library and RNA-seq were performed by the Genomics Core Laboratory at Weill Cornell Medicine using Illumina Stranded mRNA Sample Library Preparation kit (Illumina), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared from purified RNA for two or three biological replicates using TruSeq RNA Library Prep kit v2 (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 or Illumina NovaSeq.
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bioRxiv - Physiology 2023Quote: ... mRNA-Seq libraries were then prepared using the Illumina Nextera XT DNA Library Preparation kit (Illumina Cat # FC-131-1024). 1 ng of cDNA was used as input and 12 amplification cycles were used during PCR enrichment ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were constructed using the Illumina Stranded Total RNA library preparation protocol and rRNA was removed using the Ribo-Zero Plus rRNA depletion kit (Illumina). Libraries were sequenced on the Illumina NovaSeq 6000 platform (paired-end ...
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bioRxiv - Plant Biology 2023Quote: ... The RNAseq library for each sample was prepared with a 1250 ng of total RNA using the TruSeq RNA Sample Preparation kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... tuberculosis gDNA was fragmented with a Covaris S220 ultrasonicator and a whole genome library was prepared using the TruSeq Nano library preparation kit (Illumina). Library fragments containing Tn junctions were PCR-amplified from the whole genome library using the Tn-specific primer Mariner_1R_TnSeq_noMm and Illumina p7 primer (Table S4) ...
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bioRxiv - Systems Biology 2024Quote: ... The sample libraries were pooled and sequenced on an Illumina Next Seq 500 (single end 36 bases) using TruSeq SBS kit v3 (Illumina) according to the manufacturer’s protocols.
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were made according to the manufacturer’s directions for the TruSeq Stranded mRNA Library Prep Kit (Illumina, Cat. No. 20020594), and TruSeq RNA Single Indexes Sets A and B (Illumina Cat ...
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bioRxiv - Genomics 2024Quote: ... or fully concentrated samples were processed into a short-read libraries using the TruSeq Stranded mRNA Sample Prep Kit from Illumina, Inc (Cat# ...
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bioRxiv - Biochemistry 2024Quote: ... 800 ng of total RNA was used for preparing poly A libraries with an Illumina Stranded mRNA Prep Ligation kit (Illumina), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The pooled RNA-seq libraries were subjected to two rounds of 50 bp paired end sequencing on a NextSeq 550 platform using a P2 100-cycle kit (Illumina).
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bioRxiv - Molecular Biology 2024Quote: TPR-MET and TPR-METΔEx14 libraries were then prepared for deep sequencing using the Nextera XT DNA Library Prep kit in a 96-well plate format (Illumina). Manufacturer’s instructions were followed for each step ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted RNAs were ribodepleted by using the rRNA removal section of a TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat kit (Illumina), with the supernatant from the magneticbead separation cleaned up by using a Zymo RNA Clean & Concentrator kit with 8X ethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... paired-end cDNA libraries were prepared from 10 μg of total RNA by polyA selection using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... isolated RNA samples were deprived of ribosomal RNA with Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, San Diego, CA) and processed into a cDNA library for Illumina with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... Jude Hartwell Center Genome Sequencing Facility provided NGS sequencing using single end 100 cycle kit on a NovaSeq 6000 (Illumina). NGS data were analyzed using MAGeCK-VISPR/0.5.7 (29) ...
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bioRxiv - Microbiology 2024Quote: ... The RNA was dephosphorylated in 3’ and then phosphorylated in 5’ to generate cDNA libraries using the NebNext Small RNA Sample Prep kit with 3’ sRNA Adapter (Illumina) according to the manufacturer’s protocol with 12 cycles of PCR amplification in the last step followed by DNA purification with Monarch PCR DNA cleanup kit (NEB) ...
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bioRxiv - Genomics 2024Quote: ... Sequencing was performed using the massively parallel sequencing on the Novaseq 6000 platform using Novaseq S4 PE 100 kits (Illumina) to capture the expression of ∼200-5,000 genes/nuclei.
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bioRxiv - Plant Biology 2024Quote: ... 1 ug of total RNA was used for preparing each stranded library by mRNA enrichment following manufacturer indications (TrueSeq kit from Illumina). Libraries were sequenced on a Novaseq 6000 device ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were pooled and sequenced (single-end 75-bp reads) on Illumina NextSeq 500 using the High Output V2 kit (Illumina). Sequencing data were analyzed as described previously76 (additional details are provided in Supplementary Methods).
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared with the TruSeq Stranded Total RNA LT or TruSeq Stranded Total RNA-seq Sample Prep Kit from Illumina and sequenced paired-end for 6-hour and 72-hr ...
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bioRxiv - Neuroscience 2024Quote: ... Single nuclei were isolated from frozen brain tissue using iodixanol-based density gradient centrifugation and submitted to UCLA Technology Center for Genomics and Bioinformatics for library preparation (via Chromium Single Cell 3’ v3 kit from 10x Genomics) and sequencing (via NovaSeq 6000 S2 platform from Illumina). Cell Ranger (10x Genomics ...
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bioRxiv - Neuroscience 2024Quote: ... DNA libraries suitable for sequencing were prepared from 300-500 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer’s instructions (1/2 volume) ...
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bioRxiv - Cancer Biology 2024Quote: ... and the 10x Genomics recommended sequencing parameters were used to run on the NovaSeq 6000 with S4 PE 100 kits (Illumina).
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bioRxiv - Cell Biology 2024Quote: ... Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/88 run using the NovaSeq 6000 SP Reagent Kit (100 cycles) (Illumina). FASTQ files from sequencing (NovaSeq ...
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bioRxiv - Biochemistry 2024Quote: ... San Francisco Genomics CoLab using a Miniseq high output 150 cycles kit for paired end reads (Illumina FC-420-1002). Samples were demultiplexed and analysis performed using the University of California ...
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bioRxiv - Microbiology 2024Quote: ... RNA-Seq libraries were prepared using the Illumina stranded total RNA prep ligation with Ribo-Zero plus kit (Illumina, 20040529) and IDT for Illumina RNA UD Indexes (illumina ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted with a bead-beating + enzymatic lysis manual extraction method and sequencing libraries were prepared using ¼ volume reactions of the Illumina DNA Prep kit and Nextera Unique Dual Indexes (Illumina), as previously described (20) ...
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bioRxiv - Microbiology 2024Quote: Extracted nucleic acids were used for library preparation using the Nextera XT DNA library preparation kit with 15 amplification cycles (Illumina) and subjected to paired-end sequencing in a NextSeq 550 device with the read length of 150 bp at each end ...
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bioRxiv - Neuroscience 2024Quote: ... DNA libraries suitable for sequencing were prepared from 500 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer’s instructions (optimized for ½ volume reactions) ...
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bioRxiv - Microbiology 2024Quote: ... The library pool was diluted and denatured according to the standard NovaSeq protocol and sequenced to generate paired-end 152 bp reads using a NovaSeq 6000 SP reagent kit v1.5 (300 cycles) (Illumina, 20028400). After sequencing ...