Labshake search
Citations for Illumina :
6301 - 6350 of 7070 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Tagmentation by Tn5 was performed using reagents from the Nextera DNA Sample Preparation Kit (FC-121-1030, Illumina; San Diego, CA) as previously described [5] ...
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bioRxiv - Genomics 2021Quote: ... Sequencing was performed using paired end 150bp (average short-insert about 350 bp) on Illumina 2500 (Illumina, San Diego, California, USA) at Beijing Novogene bio Mdt InfoTech Ltd (Beijing ...
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bioRxiv - Immunology 2021Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented by the Nextera XT transposome ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1.1 micrograms of extracted DNA was utilized as starting material for TruSeq DNA PCR-Free library preparation kit (Illumina Inc., USA). The DNA was fragmented using focused ultrasonicator (Covaris M220 ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon sequencing was performed on an Illumina MiSeq platform using Reagent Kit v2 [2 x 300 cycles] (Illumina Inc., CA, US). Demultiplexing and trimming of Illumina adapters and barcodes was done with Illumina MiSeq Reporter software (version 2.5.1.3).
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bioRxiv - Microbiology 2020Quote: ... Pair-end sequencing (2 × 150bp) was performed using an Illumina NovaSeq or Illumina NextSeq sequencing systems (Illumina, San Diego, CA, USA). The pipeline used to separate the sequencing output into 150-base-pair pair-end read FASTQ files by library and to load files onto an Amazon Web Service (AWS ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing of the resulting library was carried out from both ends (2×300 bp) with the 600-cycle MiSeq Reagent Kit v3 on MiSeq (Illumina, USA) and the adapters trimmed from the resulting reads at the facility.
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bioRxiv - Microbiology 2020Quote: ... 5 μg of DNase-treated total RNA was first subjected to ribosomal RNA removal (RiboZero™ rRNA Removal for Gram-positive bacteria, Illumina). rRNA depleted samples were then subjected to RNA fragmentation using the Ambion RNA fragmentation kit (AM8740) ...
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bioRxiv - Microbiology 2020Quote: ... The preparation of DNA libraries was performed using the Nextera® TM DNA Library PrepKit (Illumina Inc., San Diego, CA, USA) according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... Equimolar pooled library was sequenced on an Illumina MiSeq platform using 2×300bp reagent kit (Miseq reagent kit v3; Illumina Inc.) for paired-end sequencing ...
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bioRxiv - Immunology 2020Quote: ... Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented ...
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bioRxiv - Immunology 2020Quote: ... Final loading concentration of 1.7 pM and 1% (v/v) PhiX was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina Samples were sequenced paired-end with 75 cycles of each read and single index of 6 cycles.
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bioRxiv - Microbiology 2021Quote: ... Whole-genome sequencing was performed by GATC Biotech (Konstanz, Germany) with paired-end libraries on an Illumina HiSeq platform (Illumina, USA) generating reads of 151bp in length.
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bioRxiv - Genomics 2021Quote: ... and 750 ng per sample of bisulfite (BS)- or oxidative bisulfite (oxBS)-converted DNA was run on Infinium HumanMethylationEPIC (EPIC) BeadChips (Illumina Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 10 nM of the cDNA library from every single cell was treated with the NovaSeq 6000 S4 reagent kit and sequenced by Nova-Seq 6000 (151 bp, paired-end) (Illumina KK).
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bioRxiv - Genomics 2021Quote: ... Purified DNA was subjected to both long-read (Nanopore GridION-X5 sequencer, Oxford Nanopore Technologies, Oxford, UK) and short-read (Illumina sequencer) sequencing.
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bioRxiv - Genomics 2021Quote: For miRNA expression analysis 2 µg of the small RNA was polyadenylated using the Poly(A) Tailing kit (epicenter-An Illumina company) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... value of the samples used for library preparation was ≥8 and cDNA libraries were prepared using IlluminaTruSeq Stranded mRNA Sample Prep kit (Illumina, USA) from 4μg of total RNA according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... The total RNA was transformed into a library of template molecules using TruSeq® Stranded mRNA Sample Preparation Kits (Illumina®) and the EpMotion 5075 (Eppendorf ...
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bioRxiv - Immunology 2022Quote: ... The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina, San Diego, CA 92122 USA). HLA alleles and genotypes were called using the Omixon HLA Explore (version 2.0.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction (1 ng for each sample) was used for whole-transcriptome library preparation using ScriptSeq v2 RNA-Seq kit (Illumina, USA). Libraries amplified in 14 PCR cycles were purified using Agencourt AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Bioengineering 2022Quote: ... 200 ng of total RNA was used to make RNA sequencing libraries using the TruSeq Stranded mRNA Prep Kit (Illumina 20020595). Libraries were run on a 51-cycle NovaSeq (SP flowcell ...
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bioRxiv - Bioengineering 2022Quote: ... The primers T7_Endo_Lib_LONG_FOR GCCCTCTGTGTGAATTCT and T7_Endo_Lib_LONG_REV GTCACCGACACAAGCTTA were used and a second round of PCR was carried out with the IDT for Illumina UD indexes (Illumina Corp.) to add adapter tags ...
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bioRxiv - Cell Biology 2022Quote: ... Transcriptome sequencing was then performed using an Illumina NextSeq 500 following library preparation with a Stranded mRNA Library Prep kit (Illumina, #20020594) with TruSeq RNA Single Indexes Set A (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... The ribo-zero treated RNA was used to create multiplexed RNA-Seq libraries using the NEBNext Ultra RNA Library Prep Kit (Illumina E7530) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: To augment WES variant calling an amplicon approach was also applied on 54 genes using TruSight myeloid sequencing panel[41] (Illumina, CA) and paired-end sequencing runs performed on a MiSeq (Illumina ...
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bioRxiv - Genomics 2022Quote: ... was submitted to Novogene (www.en.novogene.com) for two lanes of PE150 on an Illumina HiSeq X-Ten (Illumina, San Diego, CA, USA) sequencer.
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bioRxiv - Genomics 2022Quote: ... The final Hi-C DNA-Seq library was submitted to Novogene (www.en.novogene.com) for one lane of PE150 Illumina HiSeq X-Ten (Illumina, San Diego, CA, USA) sequencing.
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bioRxiv - Genomics 2022Quote: ... After purifying the Sample Tag PCR1 product indexing PCR was done following instructions of the MULTI-seq protocol where small RNA TrueSeq indexing primers (Illumina #15004197) were used for i7 and the Forward Primer from the BD WTA kit was used for i5.
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bioRxiv - Immunology 2022Quote: ... multiplex PCR was used to amplify rearranged VDJ sequences followed by high throughput sequencing using Illumina technologies (Illumina, San Diego, CA). PCR amplification bias was minimized by internal controls in the ImmunoSEQ assay29 ...
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bioRxiv - Genomics 2022Quote: ... The size of Nextera libraries after being purified with AMPure XP Beads was determined using a 4200 TapeStation System and quantified with Qubit dsDNA HS Assay.1.8 pM of pooled libraries was sequenced on Illumina NextSeq 550 sequencer using Nextseq 550 High Output v2 kit (75 cycles) (Illumina, #20024906) in paired-end mode ...
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bioRxiv - Genetics 2022Quote: ... DNAm in both of these groups of trios was measured using the Illumina Infinium Methylation EPIC BeadChip (Illumina, San Diego, USA) which houses ~850 000 CpG sites ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA-seq analysis was performed by single-end sequencing (66 bp) using a HiSeq 2500 instrument (Illumina, San Diego, CA, USA). Three replicates (i.e. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each sample was sequenced using 150 bp paired-end reads on an Illumina Novaseq 6000 S4 flowcell (Illumina, San Diego, CA).
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bioRxiv - Evolutionary Biology 2022Quote: ... of the gDNA was performed at the Vanderbilt Technologies for Advanced Genomics (VANTAGE) facility using the NovaSeq 6000 platform (Illumina, Inc.) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... Total DNA was used to construct Illumina paired-end sequencing library according to the manufacturer’s standard protocol (Illumina, San Diego, CA). Then ...
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bioRxiv - Microbiology 2022Quote: ... The Nextera XT DNA Library Preparation Kit was used for the library preparation according to the manufacturer’s instructions (Illumina, Cambridge, UK). Salmonella genomes were sequenced as short reads using an Illumina MiSeq 300 bp paired-end sequencer (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Its DNA was extracted from a liquid culture as explained above and sequenced using both short (paired-end Illumina HiSeq 2500) and long (Nanopore MinION ...
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bioRxiv - Immunology 2022Quote: ... was used to generate full-length cDNA prior to library preparation with the Nextera XT DNA Library Prep Kit (Illumina, Inc.). After pooling ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of total RNA was used to make libraries with the TruSeq Stranded Total RNA Gold sequencing kit (Illumina 20020598). Two modifications to the manufacturer’s protocol were made ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA was sent for library construction with TruSeq DNA PCR-free library prep kit with 350-bp insert (Illumina, USA) prior to sequencing with Illumina HiSeq 2500 platform on rapid run mode generating 2×250 bp (Macrogen Inc ...
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bioRxiv - Neuroscience 2022Quote: ... Tagmentation by Tn5 was performed using reagents from the Nextera DNA Sample Preparation Kit (FC-121-1030, Illumina; San Diego, CA) as previously described (Buenrostro et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Single-end sequencing (i.e, 1×75bp) was performed in a single run on an Illumina NextSeq 500 (Illumina, San Diego, CA). Three independent experimental replicates were run for the Cebpg shRNA-mediated knockdown and shRNA control transgenic lines for a total of 6 libraries.
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bioRxiv - Neuroscience 2022Quote: ... High-throughput sequencing was performed as single-end 75 sequencings using a NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA). SENSE 3′ mRNA-Seq reads were aligned using Bowtie2 version 2.1.0 (41) ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from the final Δrv3645 RLC12 library stock and library quality was validated by deep sequencing (see Genomic DNA extraction and library preparation for Illumina sequencing).
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bioRxiv - Microbiology 2022Quote: ... the index PCR step was performed as in the protocol except using IDT for Illumina DNA/RNA UD Indexes Set A (Illumina, 20027213), MyFi Mix (BIOLINE ...
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bioRxiv - Microbiology 2022Quote: ... and the cleaned pool was sequenced on the MiSeq using v2 2×250 base pair kit (Illumina, Inc, San Diego, CA).
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bioRxiv - Molecular Biology 2022Quote: ... 1-2 μg of total RNA was depleted of rRNA and globin mRNA using the Globin-Zero Gold rRNA Removal Kit (Illumina: GZG1224) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... A HiSeqX system was applied and 2 × 150 base read pairs were generated using standard settings (Illumina, San Diego, CA, USA). Preprocessing was performed as described by Priestley et al (26) ...