Labshake search
Citations for Illumina :
6551 - 6600 of 7070 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Genotyping was carried out using the Illumina Infinium Global Screening Array 1.0 with MDD and Psych content (Illumina, San Diego, CA, USA) at the Life & Brain facilities ...
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bioRxiv - Microbiology 2020Quote: ... the clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumina, Inc. USA) according to the manufacturer’s instructions and the library preparations were sequenced on an Illumina Hiseq 2500 platform and paired-end reads were generated.
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bioRxiv - Microbiology 2021Quote: ... The QIAseq FastSelect 5S/16S/23S rRNA removal kit was used to treat samples prior to library prep with the TruSeq Stranded Total RNA Library Prep Kit (Illumina, CA, USA). Sequencing was conducted on the Illumina HiSeq 4000 at 50 bp single-end reads.
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bioRxiv - Developmental Biology 2020Quote: ... The resulting crude nuclear pellet was then resuspended in 50 ul of tagmentation solution (1x TD buffer (Illumina, San Diego, CA 20034197), 33% PBS ...
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bioRxiv - Microbiology 2019Quote: Tag-encoded 16S rRNA gene amplicon sequencing was performed on a Illumina NextSeq using v2 MID output 2×150 cycles chemistry (Illumina, CA, USA). DNA extraction and library building for amplicon sequencing was performed in accordance to Krych et al ...
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bioRxiv - Plant Biology 2019Quote: ... Library preparation was carried out according to manufacturers instructions (NEB Next Ultra DNA Library Prep Kit for Illumina, New England BioLabs Inc) and paired end sequencing was performed on the Illumina platform at the Cold Spring Harbor laboratory (New York ...
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bioRxiv - Genomics 2019Quote: ... Genotyping for the discovery cohort was performed using the Illumina Global Screening Array-24 v1.0 BeadChip (Illumina, Inc., San Diego, CA, USA). Quality control for the SNP and individuals are described in the supplemental methods ...
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bioRxiv - Genetics 2021Quote: WGS was performed at the Health2030 Genome Center in Geneva on the Illumina NovaSeq 6000 instrument (Illumina Inc, San Diego CA, USA), starting from 1 μg of whole blood genomic DNA and using Illumina TruSeq DNA PCR-Free reagents for library preparation and the 150nt paired-end sequencing configuration ...
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bioRxiv - Genetics 2020Quote: ... Sequencing was conducted on NovaSeq6000 using 150-bp paired-end conditions according to the manufacturer’s standard workflow (Illumina, San Diego, CA, USA).
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bioRxiv - Genetics 2020Quote: ... One sequencing experiment (Experiment 1) was performed according to the manufacturer’s directions for the TruSeq Small RNA Sample Preparation Kit (Illumina, RS-200-0012). The protocol was adapted to incorporate a 2S blocking DNA oligo for removal of prevalent Drosophila small ribosomal RNA from the sequencing library [67] ...
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bioRxiv - Genetics 2021Quote: ... 300 ∼ 420 ng of RNA was used for E8.5 cDNA library generation with the KAPA-Stranded mRNA-seq kit (Illumina Platforms, KR0960-v5.17).
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bioRxiv - Genomics 2020Quote: ... The cDNA library was generated from 1 µg of total RNA using TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... RNAseq of whole tendons was conducted by the University of Michigan Sequencing Core using HiSeq 2500 system (Illumina, San Diego, CA, USA) with 50bp single end reads ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cDNA was synthesized using random hexamers and reverse transcriptase (included in the NEB Next® Ultra™ RNA Library Prep Kit for Illumina). After the first-strand synthesis ...
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bioRxiv - Microbiology 2021Quote: Indexing of purified PCR products was performed using the Nextera XT Index kit v2 (Set D) as per manufacturer’s instruction (Illumina Inc, CA, USA). The libraries after indexing were quantified using Qubit dsDNA BR assay kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The Illumina RNA-seq cDNA library was prepared using 1 μg of RNA from each sample and TruSeq V2 kits (Illumina, CA, USA), with reduced RNA fragmentation time (3 minutes ...
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bioRxiv - Immunology 2020Quote: ... One microgram of high quality RNA (RIN>8) was used as a template for library generation using the Illumina TruSeq RNA Sample Prep Kit v2 (Illumina; CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... was prepared into barcoded sequencing libraries using the TruSeq Stranded Total RNA kit with Ribo-Zero Plus rRNA Depletion (Illumina, Cambridge, UK) following manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... 300 pg of each sample was enzymatically fragmented and indexed using a Nextera XT DNA Library Preparation Kit (Illumina FC-131-1024), and Index Kit (Illumina FC-131-1001) ...
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bioRxiv - Genomics 2021Quote: ... ~800 ng of total RNA was depleted of rRNA using the Ribo-Zero rRNA Removal Kit (Illumina, Inc., San Diego, CA, USA), sheared ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell pellet was resuspended in 50 μl transposition mix (25 μl 2X TD buffer, 2.5 μl transposase (Illumina, FC-121-1030), 16.5 μl PBS ...
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bioRxiv - Genetics 2022Quote: ... WGS was performed at the New York Genome Center and Northwest Genomics Center on a HiSeq X system (Illumina, San Diego, CA) using a paired-end read length of 150 base pairs (bp) ...
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bioRxiv - Cancer Biology 2022Quote: ... using MiSeq V2 300 cycle reagent kit after the amplified library was purified with AMPure XP beads (Beckman Coulter, CA, USA) (Illumina, CA, USA) (10).
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bioRxiv - Bioengineering 2022Quote: ... the organoid was then resuspended in a direct permeabilization/tagmentation mastermix (25μl TD buffer (from Illumina Nextera XT DNA Library Prep Kit), 2.5μl Tn5 ...
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bioRxiv - Genomics 2022Quote: ... The construction of libraries was performed with Illumina preparation kits (Table S1) following the manufacturer’s recommendations (Illumina Inc., San Diego, CA, USA). The Nextera DNA Prep kit and Illumina DNA Prep were used for WGS ...
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bioRxiv - Genetics 2022Quote: ... gDNA of 293T cells with or without transfection was tested by WGS using the Illumina NovaSeq platform (Illumina, San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... Amplified (1 ng) cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). The size of the libraries was checked using the Agilent 2100 Bioanalyzer High Sensitivity DNA chip and their concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... They were collected and their genomic DNA was pooled and analyzed on an Illumina MiSeq bench-top sequencer (Illumina, San Diego, CA). Insertion sites (which included 35% of TA sites ...
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bioRxiv - Molecular Biology 2022Quote: ... After quality control and equimolar pooling paired-end sequencing of the libraries was performed on a NextSeq (Illumina, San Diego, CA, USA) instrument with NextSeq 500 High Output v2 Kit to generate 2×150 read lengths according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Whole genome sequencing was performed by the Microbial Genome Sequencing Center (MIGS, Pittsburgh, PA) using Illumina NextSeq 2000 (Illumina, San Diego, CA). Reads were aligned to the B ...
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bioRxiv - Plant Biology 2023Quote: ... The nuclei pellet was then immediately resuspended in 25 μ L of 2x tagmentation buffer containing 2 μ L of TDE1 (Illumina). The reaction was placed at 37C for 30 minutes with gentle mixing three times ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of total RNA was used as input for the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., San Diego, CA) and the manufacturer’s protocol was followed ...
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bioRxiv - Microbiology 2022Quote: The V3–V4 region of the 16S rRNA amplicons was subjected to high-throughput sequencing using a MiSeq system (Illumina, United States) and MiSeq Reagent Kit v2 (500 cycles ...
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bioRxiv - Microbiology 2022Quote: ... Amplified cDNA was fragmented and appended with dual-indexed barcodes using Illumina Nextera XT DNA Library Prep kits (Illumina, San Diego, CA). Libraries were validated on an Agilent 4200 TapeStation (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... enteroids were suspended in RNALater and RNA was isolated using Tru-Seq total RNA Sample Preparation v2 kit (Illumina, Great Abingdon, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 150 pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2024Quote: ... Extracted DNA was PCR amplified with a forward primer binding to the end of the leader sequence (PCR1 Fwd primer with Illumina adapter overhang) and a reverse primer annealing to the intronic region directly 3’ of the J segment (PCR1 Rev primer with Illumina adapter overhang) ...
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bioRxiv - Physiology 2023Quote: ... high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing ...
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bioRxiv - Cancer Biology 2023Quote: ... 4) tagmentation was performed with 2ng input and sequencing library generated using the Nextera XT library prep kit (Illumina, #FC-131-1024). In short ...
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bioRxiv - Physiology 2024Quote: ... Based RNA integrity value above 7 determined by the spectrofluorometric measurement RNA samples was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Library preparation was done for each sample fitted with one of 96 adapters with different 8 base molecular barcode for high level multiplexing and following 15 cycles of PCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... An equal amount of DNA from each library was pooled and sequenced using 150 bp paired-end settings on an NextSeq550 platform (Illumina, CA, USA).
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bioRxiv - Neuroscience 2024Quote: The Total RNA Library was prepared using the Illumina NovaSeq (S4) 300 cycle flow cell and reagent kit (Illumina, San Diego, CA). The high-throughput sequencing strategy included 300 cycles and a read length of paired-end 150 base pairs ...
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The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental developmentbioRxiv - Genetics 2024Quote: ... The Illumina Global Screening Array platform was used (v3.0) (Infinium HTS Assay Reference Guide (# 15045738 v04) (Illumina, Inc. San Diego, CA, USA). Output data were analyzed in Illumina Genome Studio version 2.0 ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA extracted from these samples was used for subsequent library construction using the TruSeq Nano DNA LT Library Preparation Kit (Illumina Inc., USA) whose purity and size were validated using an Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: HMW DNA from 1.5 x 106 cells was used to generate Illumina libraries with the Nextera XT DNA Library Preparation kit (Illumina FC-131-1024) and Illumina DNA PCR-Free Library Prep ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 1 ng/μl and subjected to library preparation using Nextera XT DNA Library Preparation Kit (Illumina, CA, USA) according to the manufacturer’s instructions and sequenced with NextSeq550 using Mid Output Kit v2.5 (300 cycles ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed on an Illumina NovaSeq 6000 instrument using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles) (Illumina, cat. no. 20028317), in a pair-end 2x100 cycle mode ...
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bioRxiv - Immunology 2024Quote: Library preparation was performed starting from 200ng total RNA of each sample using the TruSeq Stranded mRNA Library Kit (Illumina, cat. # 20020595) and the TruSeq RNA UD Indexes (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing was performed on an Illumina NovaSeq 6000 platform with NovaSeq 6000 SP Reagent Kit v1.5 100 cycles (Illumina, cat. no. 20028401). RNA seq experiments were conducted with three independent biological replicates.
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bioRxiv - Microbiology 2023Quote: ... The prepared library was sequenced using a MiSeq sequencing system with a V3 reagent kit (300 × 2 bp; Illumina, 4–6 samples). Lake Biwa viral contigs/genomes (LBVs ...