Labshake search
Citations for Illumina :
551 - 600 of 775 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... ScRNA-seq libraries were prepared using the Next GEM Chromium single cell 3′ reagent kits V3.1 with feature barcode technology for cell surface protein (10x genomics) and sequenced using NextSeq 500 high output kits and Novaseq S4 PE100 kits (Illumina). scRNA-Seq data after standard quality control was aligned to the reference genome (mm10 ...
-
bioRxiv - Cell Biology 2019Quote: ... Libraries were barcoded and sequenced on a NextSeq Series Sequencing System (HUJI Center for Genomic Technologies) using Illumina flow cell (Illumina 500 NextSeq High Output v2 Kit ...
-
bioRxiv - Immunology 2020Quote: ... Libraries were pooled and sequenced using the recommended 10X protocol for Illumina on the Mid Output flow cell for NextSeq 500 (Illumina).
-
bioRxiv - Molecular Biology 2020Quote: Small RNAs from Aag2 cells (input) or PIWI immunoprecipitations were cloned with the TruSeq small RNA sample preparation kit (Illumina) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... nuclei were extracted from 50,000 cells per sample and chromatin was tagmented using the TDE1 transposase provided in the Nextera DNA Library Preparation Kit (Illumina). While the original protocol recommended 2.5µL of enzyme ...
-
bioRxiv - Genomics 2019Quote: ... Control and NF-YA KD cells were used as input material for the TruSeq Ribo Profile Mammalian Library Prep Kit (Illumina) following the manufacture’s protocol.
-
bioRxiv - Genomics 2019Quote: Single cell calling card library preparation was performed using the Nextera Mate Pair Sample Prep Kit (Illumina #FC-132-1001) with modifications to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... the post-PCR whole transcriptome amplification (WTA) products from each single cell were transformed into sequencing libraries using a Nextera XT kit (Illumina) and unique 8-bp DNA barcodes ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... incognita isolates by 2×150 bp paired-end Illumina NextSeq 500 sequencing with High Output Flow Cell Cartridge V2 (#15065973, Illumina) and High Output Reagent Cartridge V2 300 cycles (#15057929 ...
-
bioRxiv - Neuroscience 2020Quote: ... Libraries were multiplexed on two high-output flow cells and sequenced (75 bp reads) on a NextSeq 500 device (Illumina). The mean ± standard deviation number of reads per sample meeting the Illumina quality criterion was 23 ± 6 million.
-
bioRxiv - Immunology 2021Quote: ... The cDNA was enriched for V(D)J cDNA using the Human B cell Chromium Single Cell V(D)J Enrichment Kit (Cat#1000016) followed by library construction to add the priming sites used by Illumina sequencers ...
-
bioRxiv - Immunology 2020Quote: ... and sequenced across 75 base pairs (bp) using a paired-end strategy with a 150-cycle high-output flow cell on a NextSeq 550 (Illumina). Three biological replicates were sequenced per experiment ...
-
bioRxiv - Microbiology 2021Quote: ... The pooled SynCom amplicon libraries were spiked with 25% PhiX control DNA and paired-end sequenced on an Illumina MiniSeq instrument with the mid–output flow cell (Illumina). NatCom libraries and a sample of the SynCom starting inoculum were sequenced on a MiSeq platform with 300 cycles MiSeq v3 paired-end sequencing at the Lausanne Genomic Technologies Facility ...
-
bioRxiv - Immunology 2020Quote: ... Single cell RNA libraries were prepared according to the 10x Genomics Chromium Single Cell 3’ Reagent Kits v2 User Guide and sequenced on a HiSeq4000 (Illumina).
-
bioRxiv - Genomics 2021Quote: ... cells and CD14+ monocytes were generated using the SureCell WTA 3′ Library Prep Kit for the ddSEQ System (20014280, Illumina) with the following modifications ...
-
bioRxiv - Cell Biology 2020Quote: ... The pool of libraries was loaded onto one lane of an Illumina HiSeq 4000 flow cell and sequencing was performed in a 2 × 150 bp paired end format using HiSeq 4000 SBS reagents (Illumina) and approximately 312 million acceptable quality reads were achieved ...
-
bioRxiv - Physiology 2020Quote: ... single-cell barcoded cDNA libraries were generated with 10X Chromium preparation system using the Chromium Single Cell 3’ Library & Gel Bead Kit v3 and sequenced by Illumina HiSeq ...
-
bioRxiv - Genomics 2020Quote: ... Replicas of each 384 sample pools were loaded onto the S4-flow cell following NovaSeq-XP workflow as per the manufacturer’s instructions (Illumina Inc). Dual indexed single end sequencing with 36bp read length was carried out on NovaSeq 6000 platform.
-
Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... 4 μg of total RNA from A549 cells was subjected to rRNA removal using the Ribo-Zero Gold kit (Illumina). cDNA sequencing libraries were then prepared using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies ...
-
bioRxiv - Neuroscience 2021Quote: Tagmentation and library preparation using 600 pg of cDNA from each plate of cells was then performed with a modified Nextera XT (Illumina) protocol ...
-
bioRxiv - Immunology 2021Quote: ... and duplicate compression was performed using the Cell Ranger software package (10x Genomics, CA, v2.1.0) and bcl2fastq2 (Illumina, CA, v2.20) according to the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2021Quote: ... and duplicate compression was performed using the Cell Ranger software package (10× Genomics, CA, v2.1.0) and bcl2fastq2 (Illumina, CA, v2.20) according to the manufacturer’s recommendations ...
-
bioRxiv - Developmental Biology 2021Quote: Extracted total RNA from GFP+ and IP cells from three different replicates were sent to Heidelberg genomic platform EMBL for high-throughput mRNA sequencing (NextSeq 500/Illumina). They generated mRNA libraries with NEB RNA Ultra kit (New England Biolabs E7770L ...
-
bioRxiv - Immunology 2020Quote: ... Libraries were sequenced to obtain more than 20 million 100 × 100 bp paired-end reads (S4 P200 flow cell and sequencing Kit; Illumina) mapping uniquely to mouse mm10 mRNA reference.
-
bioRxiv - Immunology 2021Quote: VH libraries from sorted B cells were subjected to NGS on the MiSeq platform with the reagent kit V3 2 × 300 bp paired-end (Illumina), using an input concentration of 16 pM with 5% PhiX ...
-
bioRxiv - Immunology 2020Quote: ... The two enriched libraries from direct flow cytometric cell sorting were combined into one library pool and sequenced on a NovaSeq sequencer (Illumina) with a NovaSeq 6000 S1 Reagent Kit (300 cycles ...
-
bioRxiv - Immunology 2020Quote: ... Single cell libraries from sorted samples were generated using a UMI-based droplet-partitioning platform with the Chromium single cell 3’ library and gel bead kit v2 and v3 (10X Genomics) and then sequenced using a NovaSeq S2 (Illumina). Technical information relevant to the scRNA-Seq samples is found in Table S3.
-
bioRxiv - Microbiology 2021Quote: ... The library pool was then loaded into flow cell of MiSeq Reagent Kit V2 (2 × 150 cycles) and sequenced in a MiSeq platform (Illumina), according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... libraries were also prepared using 50 ng of gDNA isolated from NaR P5 and 3D-RA DD25 cells by following the Nextera DNA Sample Preparation Guide (Illumina). The libraries were purified using the MinElute PCR purification kit (Qiagen ...
-
bioRxiv - Genomics 2021Quote: ... The final libraries containing barcoded single-cell transcriptomes were sequenced at 100 cycles using the S2 flowcell on the Novoseq 6000 system (Illumina).
-
bioRxiv - Genomics 2021Quote: ... Sequencing libraries were pooled on equimolar amounts and sequenced using 150-bp paired-end runs on the Illumina NovaSeq 6000 using S4 flow-cells (Illumina).
-
bioRxiv - Genomics 2021Quote: Single-cell libraries were prepared from 12,000 cells per time point (Figure 1A) using the SureCell WTA 3’ Library Prep Kit for the ddSEQ System (Illumina). Cells with viability >90% were collected by detaching cells from plates using Trypsin-EDTA 0.25% ...
-
bioRxiv - Genetics 2020Quote: ... Single-cell library was pooled with equal volumes and sequenced using Nextseq500/550 High Output Kit v2.5 (Illumina, cat# 20024906) on Nextseq500/550 sequencer (Illumina ...
-
bioRxiv - Genomics 2021Quote: The DNA methylome data of HLA-DR+/CD3- and CD3+ cells was analyzed using the HumanMethylation450K (450K) BeadChip (Illumina, USA) array as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... equal amount of RNA from each sample or cell types were used to generate the library using the TruSeq RNA Sample Prep Kit v.2 (Illumina). Paired-end sequencing was performed using TruSeq 3000 4000 SBS Kit v.3 (Illumina ...
-
bioRxiv - Microbiology 2022Quote: ... Passage 1 virus stocks were further produced in Vero TMPRSS2 cells and the presence of mutations was confirmed by Illumina sequencing ...
-
bioRxiv - Cell Biology 2022Quote: ... RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3’ v3 reagent kit (10x Genomics) and sequenced with the NovaSeq system (Illumina).
-
bioRxiv - Genomics 2022Quote: ... Libraries were pooled on equimolar amounts and sequenced using 150-bp paired-end runs on the Illumina NovaSeq 6000 using S4 flow-cells (Illumina).
-
bioRxiv - Evolutionary Biology 2022Quote: ... Each pool was sequenced 100 bp single-end on two flow cells of a HiSeq2500 sequencer using the HiSeq SBS sequencing kit version 4 (Illumina). Sequencing was conducted in two separate HiSeq runs to yield enough coverage per sample and later combined ...
-
bioRxiv - Developmental Biology 2022Quote: ... Barcoded cDNA libraries were multiplexed onto a TruSeq paired-end flow cell and sequenced (100-bp paired-end reads) with a TruSeq 200-cycle SBS kit (Illumina). Samples were run on an Illumina NovaSeq 6000 sequencer at the KUMC Genome Sequencing Facility ...
-
bioRxiv - Microbiology 2022Quote: ... ATAC-seq libraries were prepared from 50,000 cells per replicate using Illumina Tagment DNA Enzyme and Buffer Kit (Illumina 20034197) according to the Omi-ATAC-seq protocol (Corces et al. ...
-
bioRxiv - Systems Biology 2022Quote: We sequenced all our single-cell libraries using a NextSeq 500 with the High Output Kit v2.5 (75 cycles, Illumina, 20024906). For samples sequenced with the Single Cell 3’ Reagent Kit V3 (single index) ...
-
bioRxiv - Immunology 2022Quote: ... Sequencing was performed in paired-end mode with a S1 and S2 flow cell (100 cycles) using NovaSeq 6000 sequencer (Illumina) at NCCT ...
-
bioRxiv - Developmental Biology 2022Quote: ... Gene expression and hashtag libraries were sequenced on a NextSeq500 or NovaSeq 6000 S4 flow cell using v1 Chemistry (Illumina) and FASTQ files were generated using Cell Ranger mkfastq (10x Genomics).
-
bioRxiv - Developmental Biology 2022Quote: ... Indexed libraries were prepared using the Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10X Genomics; PN-120237) and sequenced on a NovaSeq 6000 (Illumina) to obtain a sequencing depth of 1.50E+08 paired-end reads per library ...
-
bioRxiv - Microbiology 2022Quote: ... The amplified libraries were hybridized to the Illumina flow cell and sequenced using the NovaSeq6000 sequencer (Illumina, San Diego, CA). Single end reads of 100nt were generated for each sample.
-
bioRxiv - Immunology 2022Quote: ... Sequencing was performed to generate paired-end reads (2 × 100 bp) with a 200-cycle S1 flow cell on a NovaSeq 6000 sequencing system (Illumina).
-
bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries for yeast (n = 3 per tested condition) and neuroblastoma cells (n = 3 per tested condition) were prepared according to the TruSeq stranded mRNA protocol (Illumina) and 101-bp single-end reads produced on an Illumina NovaSeq 6000 instrument ...
-
bioRxiv - Microbiology 2023Quote: ... Eighteen pM libraries were clustered in a high-output flow cell using HiSeq Cluster Kit v4 on a cBot (Illumina). After cluster generation ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting purified cDNA libraries were applied to an Illumina flow cell for cluster generation and sequenced on a NextSeq550 (Illumina) instrument in a single read 85 base format by following the manufacturer’s protocols ...