Labshake search
Citations for Bioline :
301 - 350 of 377 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... 800 ng of RNA were used for reverse transcription with the SensiFASTTM cDNA Synthesis kit (Bioline) according to the standard protocol.
-
bioRxiv - Cell Biology 2021Quote: ... The cDNA (100 ng) was analysed by quantitative PCR using SensiFASTTM SYBR Lo-ROX Kit (Bioline) in the 7500 Fast Real-Time PCR System (Applied Biosystems) ...
-
Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... 500 ng of RNA was used for reverse transcription with a Sensifast cDNA synthesis kit (Bioline). RT-qPCR was performed with LightCycler 480 SYBR Green I Master reagent with the primers listed below.
-
bioRxiv - Cancer Biology 2022Quote: ... Isolated RNA was used (1 μg) to make cDNA using SensiFASTTM Kit (Bioline Cat. Bio-65053). qPCR master mix was prepared using iTaq universal SYBR green supermix (Bio-Rad Cat ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1μL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020). For sequencing library preparation ...
-
bioRxiv - Genomics 2023Quote: ... Two microliters of cDNA was used for subsequent amplification using SensiFAST SYBR No-Rox Kit (Bioline) on an Eco™ Real-Time PCR System (Ilumina) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020). cFos primers were from9 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 ug RNA per sample was used as input to the SensiFAST cDNA Synthesis Kit (Bioline). RT-qPCR was performed in technical quadruplicate with TaqMan Fast Advanced Master Mix (Applied Biosystems ...
-
bioRxiv - Plant Biology 2024Quote: ... 200ng of total RNA was reverse transcribed with Superscript SentiFAST cDNA synthesis Kit (Bioline, London, UK). Real-time PCR reactions were performed in BIO-RAD HARD SHELL 96 well PCR Plates ...
-
Investigating the effects of prednisolone on behavior in mouse models of Duchenne muscular dystrophybioRxiv - Animal Behavior and Cognition 2024Quote: ... RNA was isolated from the cortex, hippocampus and cerebellum (n=6 for group A, all brains for group B and C) using TRIsure (Bioline, London, United Kingdom). Tissue was disrupted using tubes filled with small beads (OPS Diagnostics ...
-
bioRxiv - Neuroscience 2020Quote: ... and 10-50 ng of RNA were used for reverse transcription with SensiFAST cDNA synthesis kit (Bioline) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... and 10-50 ng of RNA was reverse transcribed with Sensifast cDNA synthesis kit (Bioline, London, UK). DRG axonal purity was assessed by RT-PCR ...
-
bioRxiv - Neuroscience 2020Quote: ... qPCR was performed with 500ng of cDNA and the SensiFast™ SYBR® No-ROX kit (Bioline) and a Lightcycler® 480/1536 (Roche ...
-
bioRxiv - Immunology 2020Quote: ... (Aurogene, Rome, Italy) according to the manifacturer’s instructions and reverse transcripted to cDNA through SuperMix kit (Bioline). qPCR was carried out on a Rotor-Gene 6000 (Corbett ...
-
bioRxiv - Cell Biology 2022Quote: ... cDNA was diluted 30-fold and 5μl were used for qPCR using SensiMix SYBR low-ROX (Bioline) with 150nM Forward and Reverse primers (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2024Quote: ... qRT-PCR analysis was performed on 50 ng of cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) with a Roche Lightcycler 96 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA per sample was used as input to the SensiFAST cDNA Synthesis Kit (Bioline), including -RT controls ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... For each gene a total of 8 clones from each plant were isolated from overnight LB cultures using an ISOLATE II Plasmid Mini Kit (Bioline) prior to sequencing with universal M13F and M13R primers by GATC Biotech (Konstanz ...
-
bioRxiv - Developmental Biology 2021Quote: ... qPCR reactions were performed in triplicate on 5ng of cDNA using SensiFAST SYBR No-ROX (Bioline #BIO-98005) on a LightCycler 96 (Roche Life Science) ...
-
bioRxiv - Plant Biology 2020Quote: ... RNA was used for reverse transcription according to the protocol of the SensiFASTtm cDNA synthesis kit from Bioline. PCR reactions were carried out with 5 μL of 100x diluted cDNA solution ...
-
bioRxiv - Microbiology 2020Quote: ... qPCR was performed with 50 ng of cDNA in each well and SensiFAST probe no-ROX mix (Bioline) using a CFX Connect system (Bio-Rad ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was synthesised using SuperScript III reverse transcriptase from RNA extracted using ISOLATE II RNA Mini Kit (Bioline) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... using 3 ng of cDNA per reaction and Sensifast SYBR No-ROX mix (Bioline Corporation, Alvinston, ON, Canada). Complementary DNA of mRNA was amplified using a pair of specific forward and reverse primers (Supplementary Table 2) ...
-
bioRxiv - Genetics 2021Quote: ... 1 µg of total RNA was reverse transcribed using SensiFAST cDNA Synthesis Kit following the manufacturer’s protocol (Bioline).
-
bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was performed in 20mL with 1mg of total RNA using the Tetro cDNA synthesis kit (Bioline). RT-negative (no enzyme ...
-
bioRxiv - Microbiology 2021Quote: ... Two micrograms of total RNA was reverse-transcribed with Sensi-FAST™ cDNA Synthesis Kit (Bioline, Memphis, USA). The amounts of p16 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 µg RNA (fractionation) or 11µl RNA (RNA immunoprecipitation) was converted to cDNA using BioScript Reverse Transcriptase (Bioline). Primers used in this study are provided in Supplementary Table 3 ...
-
bioRxiv - Developmental Biology 2022Quote: Isolation of total RNA and cDNA synthesis was performed by using the Isolate II RNA Mini Kit (Bioline) and SensiFAST cDNA Synthesis Kit (Bioline) ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was reverse-transcribed (1 mg each experimental point) by using SensiFAST cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed as described (18 ...
-
Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: ... Total RNA (1000 ng) was used in a reverse transcriptase reaction with the SensiFAST cDNA Synthesis Kit (Bioline). Quantitative PCR included 3 replicates per cDNA sample ...
-
bioRxiv - Cell Biology 2020Quote: ... Then 5 μg of the extracted RNA was reversely transcribed by utilizing Tetro cDNA Synthesis Kit (Bioline, BIO-65043) and random Hexamer Primer according to the manufacturer’s protocols ...
-
bioRxiv - Genetics 2021Quote: ... RNA was reverse-transcribed (1 µg each experimental point) by using SensiFAST™ cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed using SensiFast Sybr Lo-Rox Mix (BIO-94020 ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was made from 1 ug total RNA using Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted into 100 ul and 2 ul of cDNA was used to perform qRT-PCR ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA using Oligo (dT) and Super Script Reverse Transcriptase III (Bioline). GoTaq qPCR Master Mix (Thermofischer ...
-
bioRxiv - Microbiology 2021Quote: For quantification of cellular TMPRSS2 and GAPDH expression cDNA synthesis and qPCR were performed according to the manufacturer’s instructions using the SensiFast cDNA kit and SensiFAST SYBR qPCR kit (both from Bioline). The qPCR was run on a StepOnePlus realtime PCR cycler (Thermo ...
-
bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was made from 2 μg of RNA using the Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted 1 to 5 into RNAse free water to a total of 100 μl ...
-
bioRxiv - Genetics 2020Quote: ... cDNA was diluted 16-fold with deionized water and amplified using the SensiFAST SYBR Lo-ROX kit (Bioline, USA) for qRT-PCR analysis ...
-
bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s instructions and first strand cDNA synthesis was performed with oligo dT primers using BioScript reverse transcriptase (Bioline). GdMYBSG6 sequences have been deposited in GenBank under the following accession numbers ...
-
bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed with 1/10 diluted cDNA with the SensiFAST SYBR no-rox kit (Bioline, London, UK) and Lightcycler® 480 (Roche Molecular Biochemicals ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized utilizing a gene-specific primer designed to target the 10xSunTag socRNA and Tetro Reverse Transcriptase (Bioline). The resulting cDNA was isolated via column-based purification using the GeneJet Gel Extraction Kit (Thermo Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... qRT-PCR analysis was performed in triplicate on 50 ng of cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) with a Roche Lightcycler 96 ...
-
bioRxiv - Biochemistry 2023Quote: ... 1μg of RNA was used in a 20-μl reaction mixture by using a SensiFast cDNA synthesis kit (Bioline). For real-time PCR analysis ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline) and 400 nM of forward and reverse primers in a 15 µl final volume ...
-
bioRxiv - Plant Biology 2024Quote: ... Real-time RT-PCR was performed on 1:10 diluted cDNA using the SensiFAST SYBR No-ROX Kit (Bioline). Three biological and technical replicates were analyzed per genotype ...
-
bioRxiv - Neuroscience 2024Quote: ... 96-well plates were loaded with 15μl reaction mixture (1:25 primers, 1:3 cDNA and 1:2 SYBR-Green SensiMix (Bioline)) ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA was used to analyse differences in gene expression by qRT-PCR employing SensiMix SYBR low-Rox KIT (Bioline, Germany) along with gene specific forward and reverse primers (250 nM ...
-
Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... 1 µg RNA (iNeurons samples) or 2 µg RNA (Drosophila samples) was converted to cDNA using BioScript Reverse Transcriptase (Bioline). qRT–PCR primers were designed using Origene or Primer-BLAST and validated using a 1 in 4 serial template dilution series (standard curve with R2>0.97) ...
-
bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time PCR reactions using cDNA samples as template were carried out using a SensiFAST SYBR No-ROX Kit (Bioline) in a CFX96 Touch (BIO-RAD ...
-
bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR (qPCR) was carried out using the cDNA generated previously employing the SensiFAST SYBR mix No-Rox Kit (Bioline, UK in a Rotor-Gene 6000 real-time PCR cycler (Corbett Research ...
-
bioRxiv - Microbiology 2021Quote: ... Quantitative real time-PCR (qRT-PCR) analysis was performed in triplicate on 50 ng cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) and a Roche Lightcycler 96 ...